Journal of Patna Science College Vol. 1, 1 - 10 [2013] ISSN 2347 - 9604 IMPACT OF TWO PESTICIDES ON SERUM FREE AMINO ACID POOL OF MICE: A COMPARATIVE STUDY USING THIN LAYER CHROMATOGRAPHY. #Bipin Bihari Mishra, *Prakriti verma , #S.R.Padmadeo and #Kumud Ranjan Thakur #Post Graduate Department of Biochemistry Patna University, Patna- 800005 *Post graduate Department of Zoology, Patna University, Patna- 800005
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[email protected] Abstract : An attempt has been made to identify and quantify the serum free amino acids in normal and pesticide exposed mice. The laboratory mice (Mus musculus) were exposed to Dimethoate (o,o- dimethyl,S-methyl-carbamoyl-methyl phosphorodithioate),an organophosphate commonly known as rogor, with a dose of 20 mg/kg body weight and an organochlorine, Endosulfan (6,7,8,9,10,10hexachloro- 1,5,5a,6,9,9-hexahydro-6,9-Methano-2,4,3- benzodixathiepin-3-oxide) with a dose of 2 mg/kg body weight for 21 days. Every week blood were collected, centrifuged and serum were separated to estimate the free amino acids by Thin layer chromatography. Amino acids were located on the chromatogram with a 0.01% ninhydrin solution in acetone. Each chromatogram reveals 4-5 fractions with a wide range of amino acid such as aspartic acid, glutamic acid, serine, tyrosine, alanine, valine. The quantity and presence of each amino acid depends on the doses and the exposure of pesticides. Other amino acids were present in lower concentration and quantitative estimation was not possible. Finding indicates that the exposure of pesticide brings aiteration of free amino acids content in blood.