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(12) Patent Application Publication (10) Pub. No.: US 2003/0211987 A1 Labat Et Al US 2003O21, 1987A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2003/0211987 A1 Labat et al. (43) Pub. Date: Nov. 13, 2003 (54) METHODS AND MATERIALS RELATING TO Apr. 7, 2000 (US)........................................... O9545,714 STEM CELL GROWTH FACTOR-LIKE Apr. 11, 2000 (US)........................................... O9547358 POLYPEPTIDES AND POLYNUCLEOTDES Publication Classification (76) Inventors: Ivan Labat, Mountain View, CA (US); Y Tom Tang, San Jose, CA (US); Radoje T. Drmanac, Palo Alto, CA (51) Int. Cl." ....................... A61K 38/18; CO7K 14/475; (US); Chenghua Liu, San Jose, CA C12O 1/68; CO7H 21/04; (US); Juhi Lee, Fremont, CA (US); C12M 1/34; C12P 21/02; Nancy K Mize, Mountain View, CA C12N 5/08 (US); John Childs, Sunnyvale, CA (52) U.S. Cl. ......... 514/12; 435/69.1; 435/6; 435/320.1; (US); Cheng-Chi Chao, Cupertino, CA 435/366; 530/399; 536/23.5; (US) 435/287.2 Correspondence Address: MARSHALL, GERSTEIN & BORUN LLP (57) ABSTRACT 6300 SEARS TOWER 233 S. WACKER DRIVE The invention provides novel polynucleotides and polypep CHICAGO, IL 60606 (US) tides encoded by Such polynucleotides and mutants or variants thereof that correspond to a novel human Secreted (21) Appl. No.: 10/168,365 Stem cell growth factor-like polypeptide. These polynucle otides comprise nucleic acid Sequences isolated from cDNA (22) PCT Filed: Dec. 23, 2000 libraries prepared from human fetal liver Spleen, ovary, adult (86) PCT No.: PCT/US00/35260 brain, lung tumor, Spinal cord, cervix, ovary, endothelial cells, umbilical cord, lymphocyte, lung fibroblast, fetal (30) Foreign Application Priority Data brain, and testis. Other aspects of the invention include vectors containing processes for producing novel human Dec. 23, 1999 (US)........................................... O9471275 Secreted Stem cell growth factor-like polypeptides, and anti Jan. 21, 2000 (US)........................................... O9488725 bodies specific for Such polypeptides. US 2003/0211987 A1 =)----~--~~~~~~- Patent Application Publication Nov. 13, 2003 Sheet 3 of 6 US 2003/0211987 A1 BLASTN 2 . Oal 9MP-WashU (O5-Feb-l998 Build sol2.5 - ultra Ol: 47; 25 05-Feb-1998) Reference: Gish, Warren l994 -l997) . unpublished. Altschul, Stephen F. , Warren Gish, Webb Miller, Eugene W. Myers, and David J. Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215: 403-10. Notice: this program and its default parameter settings are optimized to find nearly identical sequences rapidly. To identify weak similarities encoded in nucleic acid, use BLASTX, TBLASTN or TBLASTX. Query= temporary sequence (2668 letters) Database: temp/seqfile. 64767. 4560.546875 1 sequences; 3095 total letters. Searching done Smallest STT High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N temporary sequence l3220 0-0 >temporary sequence Length = 3095 Minus Strand HSPs: Score s lis220 (1983.5 bits), Expect = 0 . O, P = 0 ... O Identities s 264.4/2644 (loot), Positives = 2644/2644 (look), Strand = Minus / Plus Query: 2644 TACAGACCACAATTACTATATATCTCGAATATATGGTccATCTGATTCTGCCAGCCGGGA 2585 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 452 TACAGACCACAATTACTATATATCTCGAATATATGGTCCATCTGATTCTGCCAGCCGGGA sill Query: 2584 TTTATGGGTGAACATAGACCAAATGGAAAAAGATAAAGTGAAGATTCATGGAATATTGTC 2525 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: S2 TATGGGTGAACATAGACCAAATGGAAAAAGAAAAGTGAAGATTCATGGAATATTGTC 57 Query: 2524 CAATACTCATCGGCAAGCTGCAAGAGTGAATCTGTCCTTCGATTTTCCATTTTATGGCCA 2465 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 572 CAATACTCATCGGCAAGCTGCAAGAGTGAATCTGTccTTCGATTTTCCATTTTATGGCCA 631 Query: 2464 CTTCCTACGTGAAATCACTGTGGCAACCGGGGGTTTCATATACACTGGAGAAGTCGTACA 2405 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbj ct: 632 CTTCCTACGTGAAATCACGTGGCA ACCGGGGGTTTCATATACACTGGAGAAGTCGTACA 69 Query: 2404 TCGAATGCTAACAGCCACACAGTACATAGCACCTTTAATGGCAAATTTCGATCCCAGTGT 2345 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbj ct: 692 TCGAATGCTAACAGCCACACAGTACATAGCACCTTAATGGAAATTCGATCCCAGTGT 75 Query: 2344 ATCCAGAAATTCAACTGTCAGATATTTTGATAATGGCACAGCACTTGTGGTCCAGTGGGA 2285 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbct: 752 ATCCAGAAATTCAACTGTCAGATATTTTGATAATGGCACAGCACTGTGGTCCAGTGGGA 8ll Query: 2284 CCATGTACATCTCCAGGATAATTATAACCTGGGAAGCTTCACATTCCAGGCAAcccTGCT 2225 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | shjct: 812 CCATGTACATCTCCAGGATAATTATAACCTGGGAAGCTTCACATTCCAGGCAAccCTGCT 871 Query: 2224 CATGGATGGACGAATCATCTTTGGATACAAAGAAATTCCTGTCTTGGTCACACAGATAAG 21.65 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | || Sbjct: 872 CATGGATGGACGAATCATCTTTGGATAcAAAGAAATTCCTGTCTTGGTCACACAGATAAG 931 Query: 264 TTCAACCAATCATCCAGTGAAAGTCGGAcTGTccGATGCATTTGTCGTTGTCCACAGGAT 21-05 Patent Application Publication Nov. 13, 2003 Sheet S of 6 US 2003/0211987 A1 Query: lil44 TAGTTCACTCGGAACATCTCCCGTGGACTTATCTGAAGTATGACAAGATTATAATGCTTT| | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | loss Sbct: 1952 TAGTTCACTCGGAACATCTCCCGTGGACTTATCTGAAGTATGACAAGATTATAATGCTTT 2011 Query: 1084 TGGCTTAGGTGCAGGGTTGCAAAGGGATCAGAAAAAAAAAATCATAATAAAGCTTTAGTT 1025 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbct: 2012 TGGCTTAGGTGCAGGGTTGcAAAGGGATCAGAAAAAAAAAATCATAATAAAGCTTTAGTT 2071 Query: lo24 cATGAGGGATCGACACCTTTGGTTCAAATGTTCTCTGATGTCTCAAAGATAACTGTTTTc 965 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | sbict: 2072 CATGAGGGATCGACACCTTTGGTTCAAATGTTCTCTGATGTCTCAAAGATAACTGTTTTC 2131 Query: 964 CAAAGCCGAACCCTTCACTCAAAAGAGCAATGATGAATGTCTCAAGATTGCTAAGAAA 9 OS | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | || Sbict: 2132 cAAAGCCTGAACCCTTTCACTCAAAAGAGCAATGATGAATGTCTCAAGATTGCTAAGAAA 2191 Query: 904 AACAGCCCATGCAAGAGTGAGAACAAACACAAAATAAGAGATTCACATTTTCAAAAC 84.5 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 2192 AACAGCCCATGCAAGAGTGAGAACAAACACAAAATAAGAGATTTTCTACATTTTCAAAAC 2251 Query: 844 AGATGTGTGGCAAAAGGATGTTGTTTTTCTGGTCTAGATCCATCTGTACCAACAAGTTCA 785 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | sbict: 2252 AGATGTGTGGCAAAAGGATGTTGTTTTTCTGGTCTAGATCCATCTGTAccAACAAGTTCA 2311 Query: 734 TCACTTACAGAACGAATCTTTTTATCCGTACAGGAGGTTCAAACCATGTCTGCCTCTTC 725 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbict: 2312 TcACTTTACAGAACGAATCTTTTTATCCGTACAGGAGGTTCAAAccATGTCTGccTCTTC 23.71 Query: 724. CTTGTAATGAATGACCCAGAGCTGTGACAAAATTTCCGAACAATTAGAAGGA 665 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbject: 2372 cTTTGTAATGAATGACCTTTCTATGAGCTGTGACAAAATTTCCGAACAATTAGCTAAGGA 2431 Query: 66.4 TTTGGGAAGAGGGGGTGGOAAACGGGGCTTTCTGTTTCCTGCCTCAGCATGAAAACATC 6 OS | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbct: 2432 TTTGGGAAGAGGGGGTGGCAAACGGGGCTTTCTGTTTTCCTGCCTCAGCATGAAAACATC 2491 Query: 604 TGATTTATGCTTTATGGAAGCCTTACCTCCAATCCCCAACTGTTAAGTCCCATGAAACCA 545 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | sbjct: 2492 TGATTTATGCTTTATGGAAGCCTTACCTCCAATCCCCAACTGTTAAGTcccATGAAAccA 2551. Query: 544 CAGTTGCTCTGGGCTGATGGAAACAAAAGGAAACAGTATGAAGAGTTCCTTAATCATTTT 485 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 2552 CAGTTGCTCTGGGCTGATGGAAACAAAAGGAAACAGTATGAAGAGTTCCTTAATCATTTT 25ll Query: 484 TGAAACAAAAATGTTAAGGGATAAACATATGATTATTTTTAATTTATGCCITTCA 425 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 2612 TGAAAcAAAAATGTTAAGGGATTTTAAACATATGATTATTTTTAATTTTATGCCTTTTCA 2671. Query: 424 GTACTAAACACCCATTTCATTGCTGATTCCTGTCTAAGAAGCCATTCACGTCAGCATGGC 365 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | sbict: 2672 GTACTAAACACCCATTTCATTGCTGATTCCTGTCTAAGAAGCCATTCACGTCAGCATGGC 2731 Query: 364 GATASAAAGAATGAAAAAACCCTGCSAACATACAGTAATTTTCTTAAAGCACATAG 305 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 2732 GATAGAAAGAATGAAAAAACCCTGCTGAATCATACAGTAATTTTCTTTAAAGCACATAGT 279 Query: 3O4 AGTACATAAATATATATATATAAATATATTTTTGTTTATAACTAACACAAGGCAGGATC 245 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbjct: 2792 AGTTACATAAATATATATATATAAATATATTTTTGTTTATAACTAACACAAGGCAGGATC 2B5. Query : 244 TGGACTCAAGAGTGCGTTTGCATCAAGACAAAACAGATGOAAGATGOATCACTGC 85 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Sbict: 2852 TTGTGACTCTAAGAGTGCGTTTTGTCATCAAGACAAAACAGATGCAAGATGCATCACTGC 29ll Query: 184 ATTACTTCCATAGAGTTGTAAAATAATCCTTAATATTAGAATATTTTTCTGTCACTTAGC 125 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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