Selection and demographic history shape the molecular evolution of the gamete compatibility protein bindin in Pisaster sea stars Iva Popovic1, Peter B. Marko2, John P. Wares3 & Michael W. Hart1 1Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada 2Department of Biology, University of Hawai’i, Manoa, Hawaii 3Department of Genetics, University of Georgia, Athens, Georgia Keywords Abstract Bindin, concerted evolution, gamete recognition, positive selection, sexual conflict. Reproductive compatibility proteins have been shown to evolve rapidly under positive selection leading to reproductive isolation, despite the potential Correspondence homogenizing effects of gene flow. This process has been implicated in both Iva Popovic, Department of Biological primary divergence among conspecific populations and reinforcement during Sciences, Simon Fraser University, Burnaby, secondary contact; however, these two selective regimes can be difficult to dis- BC V5A 1S6, Canada. Tel: +604 551 8950; criminate from each other. Here, we describe the gene that encodes the gamete Fax: +778 782 3496; E-mail:
[email protected] compatibility protein bindin for three sea star species in the genus Pisaster. Funding Information First, we compare the full-length bindin-coding sequence among all three spe- This work was supported by the Natural cies and analyze the evolutionary relationships between the repetitive domains Sciences and engineering Research Council of the variable second bindin exon. The comparison suggests that concerted of Canada and Simon Fraser University. evolution of repetitive domains has an effect on bindin divergence among spe- cies and bindin variation within species. Second, we characterize population Received: 19 September 2013; Revised: 15 variation in the second bindin exon of two species: We show that positive selec- February 2014; Accepted: 26 February 2014 tion acts on bindin variation in Pisaster ochraceus but not in Pisaster brevispi- nus, which is consistent with higher polyspermy risk in P.