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Liposomes As Versatile Tools: Nanoreactors, Membrane Models and Drug Delivery Carriers

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Liposomes As Versatile Tools: Nanoreactors, Membrane Models and Drug Delivery Carriers LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Liposomes as versatile tools: nanoreactors, membrane models and drug delivery carriers Gael Clergeaud Veiga Doctoral Thesis Department of Chemical Engineering 2014 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Liposomes as versatile tools: nanoreactors, membrane models and drug delivery carriers Doctoral Thesis supervised by Dr. Mayreli Ortiz and Dr. Ciara O’Sullivan Department of Chemical Engineering University Rovira i Virgili Tarragona Spain 2014 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Liposomes as versatile tools: nanoreactors, membrane models and drug delivery carriers Tribunal members: Prof. Pau Ballester (Institute of Chemical Research of Catalonia, University of Rovira i Virgili) Prof. Daniel Maspoch Comamala (Catalan Institute of Nanoscience and Nanotechnology, Autonomous University of Barcelona) Prof. Fatouros Dimitrios (Aristotle University of Thessaloniki) Prof. Alex Fragoso (University of Rovira i Virgili) Dr. César Fernández Sánchez (Barcelona Microelectronics Institute, CNM-CSIC, Autonomous University of Barcelona) Dr. Rükan Genç (University of Mersin) External examiners: Prof. Thomas Lars Andresen (Technical University of Denmark) Dr. Neil Keegan (Institute of Cellular Medicine, University of Newcastle) Department of Chemical Engineering University Rovira i Virgili Tarragona Spain 2014 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Department of Chemical Engineering Universitat Rovira i Virgili Av. Països Catalans 26 43007 Tarragona, Spain Tel: 977 55 96 58 Fax: 977 55 96 67 I STATE that the present study, entitled “Liposomes as versatile tools: nanoreactors, membrane models and drug delivery carriers”, presented by Gael Clergeaud Veiga for the award of the degree of Doctor, has been carried out under my supervision at the Department of Chemical Engineering of this university, and that it fulfils all the requirements to be eligible for the International Doctorate Award. Tarragona, 15th October 2014 Doctoral Thesis Supervisor/s Dr. Ciara O’Sullivan Dr. Mayreli Ortiz UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Acknowledgments The present doctoral thesis covers the work carried out through my three years as a Ph.D. student in the Nanobiotechnology & Bioanalysis group at the Department of Chemical Engineering of the University Rovira i Virgili. First of all I would like to thank my supervisors Dr. Ciara K. O’Sullivan and Dr. Mayreli Ortiz for their inspirational talks, great guidance and enormous support during my Master and Ph.D. thesis. They offered me the tools to construct what I am now. I would especially render my gratitude to Dr. Ciara K. O’Sullivan for believing in me for pursuing the Ph.D. within the group. I would also want to express my gratitude to Prof. Thomas L. Andresen for giving me the chance to work in his lab, for the valuable discussions and for the priceless experiences garnered. I would also like to thank all the colleagues from the Colloids & Biological Interface group at the Technical University of Denmark for the constructive and friendly environment rendered up during my stay in Denmark, especially to my colleague Houman. I could not forget to give huge thanks to all the past and present members from the Nanobiotechnology & Bioanalysis group I have met along this “trip” for the enthusiastic research and friendly atmosphere rendered. To all the technicians, administratives and postdocs for their kindly help, and to all the Ph.D. students that we shared this great experience… Special mention goes to Jonathan for those irreplaceable and insanely good moments laughing out loud. I would also like to thank all the people from the URV microscopy unit for their helpful collaboration. In general, to all those people that have added something during this stage... you know who you are. I would also want to appreciate the great support received from all my family, for always being so generous and open-minded, especially from my father Francis and my mother Teresa, whom have always believed on me and pushed me forward to achieve my goals. Infinitely grateful… Last but not least, I want to thank immensely my girlfriend Alba, for her huge support, her wise advices and her unconditional love. She has been and is the accomplice of my life, and throughout this Ph.D. trip has made even finish a thesis possible. UNIVERSITAT ROVIRA I VIRGILI LIPOSOMES AS VERSATILE TOOLS: NANOREACTORS, MEMBRANE MODELS AND DRUG DELIVERY CARRIERS. Gael Clergeaud Veiga Dipòsit Legal: T 154-2015 Agradecimientos La presente tesis doctoral abarca el trabajo llevado a cabo durante mis tres años como estudiante de doctorado en el grupo Nanobiotechnology & Bioanalysis en el Departamento de Ingeniería Química de la Universidad Rovira i Virgili. En primer lugar me gustaría dar las gracias a mis supervisores Dr. Ciara K. O'Sullivan y Dr. Mayreli Ortiz por sus charlas inspiradoras, su gran orientación y su enorme apoyo durante mi tesis de master y doctorado. Me ofrecieron las herramientas para construir este proyecto. Me gustaría agradecer especialmente a la Dr. Ciara K. O'Sullivan por creer en mí para continuar con el doctorado dentro del grupo. También me gustaría expresar mi gratitud al Prof. Thomas L. Andresen por darme la oportunidad de trabajar en su laboratorio, por las valiosas discusiones y las impagables experiencias recolectadas. También me gustaría dar las gracias a todos los miembros de su grupo Colloids & Biological Interface en la Universidad Técnica de Dinamarca por el amigable y constructivo ambiente creado durante mi estancia en Dinamarca, especialmente a mi colega Houman. No podría olvidar agradecer enormemente a todos los colegas, pasados y presentes del grupo Nanobiotechnology & Bioanalysis que he conocido a lo largo de este "viaje", por crear un ambiente agradable y mostrar una entusiasta actividad investigadora. Gracias a todos los técnicos, personal administrativo y postdocs por su enorme y amable ayuda, y a todos los estudiantes de doctorado que hemos compartido esta gran experiencia... mención especial va para Jonathan, por esos momentos irremplazables e increíblemente buenos compartiendo risas y carcajadas.
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