Proteomics B.101 Solute retention of Spectrum UF membranes membranes, ideal for standard pplications Membranes 200 MWCO most molecules with MW >200 Membranes 200 MWCO most 200 Da MWCO (cut-off, sizes...), special applications dialysis membranes Hollow fibers easily and quickly directly in the dialysis tubing! dialysis device quality/price implants Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting microvolumes sterile applications Combines Dialysis and SPE phase affinity ! Comments Highest Largest choice ultra cleaned membranes,...) Ready-to-use For Reusable Equilibrium dialysis Concentrate treated EDTA Biotech RC/CE ligands, cytokines... molecules Biomolecule of interest proteins carbon hydrates tubings ! Separation techniques (Proteins) techniques Separation SpectraPor Sheets, Discs in vivo dialysis follow filters Cell UptiTip Chromalys Products CelluSep dialysis SpectraPor dialysis tubings Float-A-lyser DispoDialyserMicroDialyser FastDialyser inquire GebaFlex units and systems For B108 of molecules discrimination are affected near the Area biochemistry (synthesis, pharmacology drugs, selectivity Page B103 CelluSep H1 and the ,... (see the table for nucleic acids with CelluSep). For effective dialysis, effective ,... (see the table for nucleic acids with CelluSep). For 000 da B104 e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] à 1 000 dialysis rate studies Also, the MWCO is defined for globular proteins (90% of molecules with MW=MWCO are retained), in given MWCO is defined for globular proteins (90% of molecules on the temperature,...). So, the membrane choice may depend conditions (buffer, be favored with MWCO far above MW of MWCO. For quick desalting, the dialysis speed may with small difference of MWs or with salts (closer to biomolecules), while for applications may be privileged by choosing a MWCO populations of dispersed molecules, the selectivity relatively close to small molecules to be removed. shape, hydrophilicity passed should be superior to 25. the ratio (MW of molecules to retain/MW of salt to remove) Applications desalting (removing salts) exchangebuffer fractionationdrug binding studies analysis) labeling, production (purification,...)acids nucleic lipids drug release in cell models or organisms drug release in cell models ! ! ! ! ! ! ! Selection guide Multiple smal sample (1µl) B185 In vivo dialysis experimentationElectro-elution (S dialysis)Concentration B115 B112 S/P B115 SpectraGel 10-100 µl / 3 500 à 60 000 Da0.1-3 ml / membrane choiceIn vitro binding B111 B112 0.5 ml to Liters / 100 (reinforced,0.3-10 ml / 100 Da to 300 000 Da0.5-3 ml / 100 Da to 300 000 Da B110 B111 DNA/RNA dialysis and Application / sample / MWCO 0.5 ml to Liters B102 Dialysis is performed by conventional tools as tubings, or flat sheets using a suitable Dialysis is performed by conventional tools as tubings, or flat sheets using a multiple support, or with specialized devices dedicated to applications for small samples, samples, in vivo applications, equilibrium studies… Dialysis is driven by a differential concentration gradient, discriminating biomolecules concentration gradient, a differential Dialysis is driven by be considered to display membrane. The membrane can across a "semi-permeable" size, and is permeable to small molecules. pores that retain molecules above a given Weight Cut-Off (MWCO) between the Membrane should be chosen with a Molecular compartment, and the MW of the molecules MW of molecules to recover in the sample reaction,...). See technical tip. to remove (usually salts, by-products of Dialysis is an easy and efficient technique to separate biomolecules according to separate biomolecules technique to an easy and efficient Dialysis is release or bioactive compounds also usefull for small weight, but their molecular include : Applications binding studies. Dialysis and Ultrafiltration Dialysis B.102 Proteomics Separation techniques(Proteins) Desalting /Dialysis,Ultrafiltration ! ! CelluSep CelluSep ! ! ! ! ! ! ! with adessiccanttocontrolhumidity. glycerol topreventbrittleness,andarepackaged CelluSep (bovine serumalbumin) Protein adsorption Vitamin B-12(1355Da)59% Cytochrome C(12,3KDa)97% (T =10hours,withT2Membranes) Retention Lead 0,75-0,8ppm Zinc 6,24-12,9ppm Nickel <0,1ppm Iron 13,6ppm Copper <0,1ppm Chromium <0,1ppm Cadmium <0,1ppm ® ® ® T1,T2,T3etT4membranescontain membranesPerformances membranestechnicaldatas Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 ≤ 1mg/cm 2 T1, T2,T3andT4CelluSep manufactured withhighqualityprocessforoptimalandreproducibleresults. applications, forproteinsandnucleicacids. They areregeneratedcellulosemembranes CelluSep Dialysis Tubings -CelluSep 0m 55m 5.10 3.27 1.67 25.5mm 20.4mm mm 15.9 14.6mm 40 mm 32 mm 25 mm 23 mm mm 29.3 mm 12.1 CelluSep Ø dry 46 mm 19 mm Flat width CelluSep ltwdhØdyV mm 28.6 mm 21.0 mm 15.9 mm 6.37 45 mm Ø dry 33 mm 25 mm 10 mm mm 48.4 mm 28.6 Flat width mm 21.0 Standard grade(withoutaccessories) mm 15.9 CelluSep mm 6.37 76 mm 45 mm Ø dry 33 mm 0.32 25 mm mm 31.8 10 mm Flat width CelluSep 6.37mm 50 mm Ø dry 10 mm Flat width CelluSep You [email protected] CelluSep membranesareprovidedwithacompletedocumentation. Accessories includeCelluSepmembraneclosureclampsandMacroFillerfunnels. packages), andDNAdesalting. suit mostexigentapplications(minimalcontaminantscontent,ready-to-useindividual levels, goodcompatibilitieandeconomiccost.H1CelluSep especially whenattentionispaidtoverylowadsorptionofproteins,impurities !! ! ! ! ! ! ! CelluSep and two clamps Each packageincludesadetailedinstructionsmanual Economic Nominal Filterratings:3500,6000and12Daltons Pure regeneratedcelluloseforlowimpuritieslevels Very lowadsorptionsofproteins ® ® ® ® T1Membranes - 3500 Da Nominal Filter rating : 4000-6000 MWCO 4000-6000 : rating Filter Nominal T1Membranes-3500Da T3Membranes-12000Da T2Membranes-6000Da T4Membranes-12000Da/standardNominalFilterrating:12-14 000MWCO) ® ® T1,T2,T3andT4tubulardialysismembranes Dialysistubingsarethebestchoicefordialysis,notablyinBiology ® T1,T2,T3etT4membranessuitmostdialysisapplications How canIconcentratedialyzedsamples? Do Ineed/howcancleanmembranes? How manytimesshouldIchangebuffer? How canIsterilizemembranes? Which volumeofdialysisbuffer? Which MWCOshouldIchoose? How longshouldIdialyze? ® Dialysis Membranes suit classical dialysisapplications, DialysisMembranes 1.98 6.74 1.15 Vol/cm Nominal Filterrating:6-8000MWCO 6.42 3.46 1.98 0.32 18.4 6.42 3.46 1.98 0.32 Vol/cm Nominal Filterrating:12-14000MWCO 7.94 Vol/cm ol/cm ... T2-25-15 T1-46-15 T1-19-15 Cat.# T2-40-15 T2-32-15 T2-23-15 T4-45-30 T4-33-30 T4-25-30 T4-10-30 Cat.# T3-76-5 T3-45-15 T3-33-15 T3-25-15 T3-10-15 Cat.# T2-50-15 Cat.# T2-10-15 ® DialysisMembranes 15 m 15 m 15 m Length 15 m 15 m 15 m 30 m 30 m 30 m 30 m Length 5 m 15 m 15 m 15 m 15 m Length 15 m Length 15 m Proteomics B.103 117-mer 117-mer 15-mer 35-mer 15-mer 35-mer DNA analysis with CelluSep H1 membrane MWCO of 2 000 and 5 000 allow desalting of any fragment size (ss or ds). 10, 15 and 20 000 MWCO membranes However, provide a faster alternative when dialysing double- stranded nucleic acids. Technical tip Technical % of retention - Single stranded DNA MWCO 0002 0005 1 00010 0.95 00015 0.50 00025 0.50 0.93 0.95 0.82 0.39 0.82 0.90 0.93 0.74 0.82 0.82 0.68 2 0002 0005 0.94 00010 0.93 00015 0.91 1 00025 0.90 1 1 0.82 0.95 1 0.86 1 1 1 1 % of retention - Double stranded DNA MWCO Facilitates tubing handling with closures, openers See accessories for Dialysis page B116 H1 ® Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting R00171 R00141 ! R00320R00330R00340R00350 R00321 R00360 R00331 R00370 R00341 R00380 R00351 R00390 R00361 R00400 R00371 R00381 R00391 R00401 Cat.# (5 m) Cat.# (10 m) R00180R00190R00200R00210 R00181 R00310 R00191 R00201 R00211 R00311 Cat.# (5 u)H1-18-5H1-38-5u) (10 Cat.# H1-45-5H2-18-5 H1-18-10 H2-38-5 H1-38-10 H2-45-5 H1-45-10 H5-18-5 H2-18-10 H5-20-5 H2-38-10 H5-32-5 H2-45-10 H5-40-5 H5-18-10 H10-10-5 H5-20-10 H10-25-5 H5-32-10 H10-32-5 H5-40-10 H10-10-10 H10-40-5 H10-25-10 H15-10-5 H10-32-10 H15-25-5 H10-40-10 H15-32-5 H15-10-10 H15-45-5 H15-25-10 H25-12-5 H15-32-10 H25-25-5 H15-45-10 H25-35-5 H25-12-10 H25-40-5 H25-25-10 H25-35-10 H25-40-10 Separation techniques (Proteins) techniques Separation ol/cm ol/cm 1.27 0.46 1.67 3.27 6.42 7.94 0.46 1.98 3.46 6.42 6.74 0.46 3.27 5.10 1.15 1.67 7.95 1.04 2.86 5.10 1.98 3.27 0.32 1.98 6.42 0.46 3.91 5.10 1.04 4.59 6.42 1.04 4.59 6.42 0.32 5.10 3.27 1.98 1.5 mm 1.5 mm 1.5 mm 19.1 mm 25.5 mm 15.9 mm 20.4 mm 6.37 mm 15.9 mm 28.6 mm 7.62 mm 22.3 mm 25.5 mm 24.2 mm 28.6 mm 24.2 mm 28.6 mm 12.7 mm 6.37 mm 25.5 mm 20.4 mm 15.9 mm e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] Flat width V H1 Membranes H1 provides an efficient tool for DNA and RNA purification, allowing both purification, allowing and RNA tool for DNA H1 provides an efficient H1 is ideal for sensitive applications where glycerol, sulfur compounds, or applications where glycerol, sulfur H1 is ideal for sensitive ® ® ® Suits protein applications, and especially DNA/RNA applications especially DNA/RNA and applications,Suits protein content for minimal contaminants Pre-treated packaged Ready-to-use individual-use 25 000 Daltons MWCO from 1 000 to ! ! ! ! High Grade 100001000015000 32 mm 1500015000 50 mm 15000 12 mm 25 mm 34 mm 45 mm 100001000010000 12 mm 24 mm 45 mm 80008000 32 mm 40 mm 35008000 46 mm 12 mm CelluSep tubings H1 in rolls MWCO 35008000 19 mm 8000 24 mm 50 mm With the important expansion of Molecular Biology and the subsequent need for nucleic of Molecular Biology and the With the important expansion dialysis membranes behavior and their high acids and proteins samples desalting, size and the three-dimensional shape of a cleanness has become important. The or not through a membrane. molecule determine whether it passes has become a (MWCO) Cut-Off globular ones, Molecular Weight For proteins, notably membrane to use. convenient guide for determining which by the length in base (single-stranded : ss) or Nucleic acids are routinely characterized proteins. (double-stranded : ds), and they display far less tertiary structure than pairs to the length of DNA provides a superior Choosing the appropriate membrane according The table below compares the nominal alternative to the MW of the DNA fragment. lengths of DNA (ss and ds). membrane MWCO for retention of various dialysis and concentration (see SpectraGel). dialysis and concentration small amounts of heavy metals will interfere with subsequent steps. CelluSep with subsequent steps. will interfere of heavy metals small amounts CelluSep membranes are EDTA pretreated and packaged wet in a preservative solution. Individual- wet pretreated and packaged membranes are EDTA and restorage concerns. avoid waste, contamination use 50 cm packages CelluSep CelluSep 2500025000 35 mm 40 mm 1500025000 45 mm 12 mm 100001500015000 32 mm 10 mm 25 mm 5000500010000 32 mm 40 mm 25 mm 20005000 45 mm 18 mm 1 10001000100020002000 18 mm 38 mm 45 mm 5000 18 mm 1 38 mm 10000 1 20 mm 10000 10 mm 15000 40 mm 25000 32 mm 25 mm CelluSep H1 tubings in Length of 50 cm MWCO Flat widthdry Ø V B.104 Proteomics Separation techniques(Proteins) Desalting /Dialysis,Ultrafiltration C1 o5k2t 260°C 12 to 2 60°C 3.5kto 50k to 1k 2 to 12 Type F 60°C RC 1k to 50k or resistancetoheat.Howeverprotein adsorptionishigher. CE RC Spectra/Por 12kto14k Biotech RC 12 to 2 Biotech CE RC They areproducedwithsharp,rigidly controlledMWCOs,andprovidedwetready-to-use. 12kto14k 60°C Biotech RCandCEmembranes arethemostpuremembranesavailabletoday. RC 7 3.5k (i.e. beforeMSanalysis,forenzymeactivities,crystallizations…).Biotech membranesareanalternativechoice. 12 to 2 Spectra/Por RC 12kto14k 6 Spectra/Por RC 8k to 6k 5 Spectra/Por RC 4 Spectra/Por RC 3 Spectra/Por 2 Spectra/Por 1 Spectra/Por Membrane Type higher. resistance toheat.Howeverproteinadsorptionis acids andbasesorganicsolvents,or have awidecompatibilitywithvariousaqueous ! CE membranes) radiation (availableinBiotechRCand ! found instandarddialysismembranes. and metalcontaminantswhicharecommonly exceptional freedomfromimpuritiessuchassulfide No specialprecleaningisrequired,thanksto eliminates theuseofmetalsaltsinmanufacturing. cations. Theyareobtainedbyaprocessthat membranes ! use, exceptfortheBiotechgrade. RC membranescouldbecleanedbefore benefit oftheirnarrowerporesize.Itisbetter may bechoseninfirstintentionwiththe ! molecular porousdialysismembranes. CE membranesexhibithydrophilicanduniform solvents atconcentrationsexceeding5%inwater. The membraneshouldnotbeexposedtoorganic damage, butwithpossiblealterationofMWCO. ethanol, andisopropanol)withoutapparent tolerate exposuretoloweralcohols(methanol, damage themembrane.Thismembranewill solvents suchasacetone,MEKordioxanewill are sensitivetoorganicsolvents.Strongpolar have tobecleanedbeforeuse.CEmembranes ! Overview ofSpectra/Por PVDF Sterilized membranes Biotech RCmembranes Regenerated cellulose Cellulose acetate ® ® ® ® ® ® membranes(Spectra/Por® Type F) 5membranesarereinforcedtowithstandhighpressuredifferentials, butdialysisisslowerthaneitherSpectra/Por ® ® Type FDialysisMembranesarerecommended whenawidecompatibilityisdesiredwithvariousaqueousacidsand basesandorgani 6membranesarerecommendedwhenconvenienceofuse,astheyin avarietyofMWCOsfrom1to50KDa. 4membranesaresimilartoSpectra/Por 3membranesarerecommendedforsmallbiomolecules(MWCO3.5KDa) 2membranesarerecommendedforclassicusesproteinsabove20KDa(MWCO12-14KDa)orquickerdialysiskinetics arededicatedtomostexigentappli- 1arerecommendedforclassicuseswithbiomoleculesabove10kD(MWCO6-8KDa) 7membranesarerecommendedforapplicationswhereimpurities (metal,disulfides)maybecrucial Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 VF8kt ,0k1t 4130°C 1 to 14 80kto2,000k PVDF Material (CE)membranesdon’t areobtainedbyir- (RC)membranes and BiotechCE 0 o30 2 to 9 100 to300k pH MWCO ® 0 o1 60°C 12 to 2 60k Membranes These membranesexist: Spectra/Por Dialysis Membranes-Spectra/Por ® !! 2,butwithlowerporedensityanddialysiskinetic Selection guide o1 60°C 60°C 12 to 2 12 to 2 60°C 12 to 2 ! ! ! ag T Range In regeneratedcellulose(RC),madeoflinters(Spectra/Por In PVDF(polyvinyldifluoride)(Spectra/Por the highqualitygradeforBiotechCEmembranes. In celluloseacetate (CE),madefromsinglecompoundsofpurecellulose,with grade BiotechRCmembranes. or naturalcelluloseandpaper (Spectra/Por trlt ihprt SpectraPorBiotechsterile SpectraPorF organic solventcontainingbuffers Sulfide crystallography studies sterility /highpurity convenience ofuse Specific applications/requirements molecules 3.5kDa- molecules 10kDa- choice intention First molecules >=20kDa Classic applicationsofdialysis Spectra/Por ® isthemosttrustedandusednameinlaboratorydialysis. ® 7CN Yes No 37°C membranes: emperature limit 0D SpectraPor3 10kDa 0D SpectraPor1 20kDa Solvent Resistance Organic otO oHighest No Most OK Mode No No Many OK Many OK No Many OK ayO e Highes Yes No Many OK No Many OK Many OK No Many OK No Many OK SpectraPor 7/minimaldisulfideandmetaltraces,Metal SpectraPor 1.2.3.4ready-to-usedialysissacks Dispodialyzers SpectraPor 6/providedwetandreadytouse SpectraPor 5forreinforcedmembranes SpectraPor 4forlowerporedensityanddialysiskinetic SpectraPor 2 removal solutions,BiotechCEandRCmembranes ® ® TypeF) ® tubings,sheets&discs 5.6.7),andwiththehighquality vial raitdDialysisrate (sterile) Available Irradiated ® 2or4. High High High High High High High ® 1.2.3.4), c solvents, rate Proteomics B.105 Facilitates tubing handling with closures, openers See accessories for Dialysis page B116 Related product : CelluSepT2. Need to clean your dialysis membranes ? heavy metal and Sulfide removal -> see page B115 Related product : CelluSepT1. Related product : CelluSepT3. Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting (100) (100) (100) (50) Package Length Package Length Package Length ! Separation techniques (Proteins) techniques Separation olume/Length Diameter V Diameter Volume/Length Diameter Volume/Length e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] 184554Flat sheets 108 mm x 108 mm, package of 25 Flat discs, 33 mm diameter, package of 50 Flat discs, 47 mm diameter, package of 50 11.5Flat discs, 100 mm diameter, package of 50 29Ready-to-use dialysis sacks for 1 to 40 mL samples, package of 10 34Ready-to-use dialysis sacks for 5 to 400 mL samples, package of 10 1.0 6.4 9.3 15 (50) 15 (50) 15 (50) Flat Width mm mm mL/cm m (ft) Ready-to-use dialysis sacks for 5 to 400 mL samples, package of 10 Ready-to-use dialysis sacks for 5 to 400 mL samples, Flat sheets 240 mm x 240 mm, package of 25 Flat sheets 240 mm x 240 package of 50 Flat discs, 33 mm diameter, package of 50 Flat discs, 47 mm diameter, package of 50 Flat discs, 100 mm diameter, for 1 to 40 mL samples, package of 10 Ready-to-use dialysis sacks Flat Width mm1023324050 mm100 6.4120 14.6 20.4 25.5 mL/cm 32 64 76 0.32 1.7 3.3 m (ft) 5.1 8.0 32 15 (50) 46 30 30 30 30 (100) 15 (50) 15 (50) 25 16 2.0 15 (50) Ready-to-use dialysis sacks for 1 to 40 mL samples, package of 10 Ready-to-use dialysis sacks for 5 to 400 mL samples, package of 10 mm61045105 mm120Flat sheets 200 mm x 200 mm, package of 25 3.8 6.4Flat discs, 33 mm diameter, package of 50 Flat discs, 47 mm diameter, package of 50 29Flat discs, 100 mm diameter, package of 50 67 mL/cm 76 0.1 0.32 m (ft) 6.4 34 46 15 (50) 15 15 (50) 15 (50) 15 (50) Flat Width 3 RC Dialysis Membranes - MWCO 3.5 kDa 1 Dialysis Membranes - MWCO 6-8 kDa Membranes - MWCO 1 Dialysis 2 RC Dialysis Membranes - MWCO 12-14 kDa 2 RC Dialysis Membranes - MWCO 12-14 2 Regenerated Cellulose (RC) membranes are recommended for 2 Regenerated Cellulose (RC) membranes 3 Regenerated Cellulose (RC) membranes are recommended for small 1 Regenerated Cellulose (RC) are recommended for classic uses are recommended Cellulose (RC) 1 Regenerated ® ® ® ® ® ® Spectra/Por 284290 180510 467660 O31460 169590 O31450 O31590 183290 195610 Cat.# Spectra/Por biomolecules. Spectra/Por 802890 834650 O31430 O31420 O31410 382700 229660 367890 O31550 409920 458210 265670 666210 with biomolecules above 10kDa. with biomolecules Cat.# Spectra/Por Spectra/Por 229900 O31560 O31570 904940 O31440 983580 220450 236260 25221R 982760 369160 216710 classic uses for proteins above 20KDa or for quicker dialysis kinetics. classic uses for proteins above 20KDa Cat.# Spectra/Por B.106 Proteomics Separation techniques(Proteins) Desalting /Dialysis,Ultrafiltration -> seepageB115 Spectrogel#292600 your dialyzedsample? You searchaneasyandquickwaytoconcentrate Related product:CelluSep T4. Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 O31470 319480 22983R 18868R Cat.# Spectra/Por 358320 O31580 O31480 548160 390530 19112R 217740 but withlowerdialysiskinetic. Spectra/Por 314250 729580 310780 834710 O31500 O31510 128118 19921R 128106 O31530 O31520 923650 750020 128058 758730 128056 758720 546530 257730 381580 265110 189840 22991R 338240 263820 Cat.# Spectra/Por 401110 314251 O31490 220340 128224 128218 128206 220330 203450 128158 128156 800370 746980 MWCOs from1to50KDa.Spectra/Por convenience ofuse,becausetheyareprovidedwetandready-to-useinavariety Spectra/Por O31650 726880 O31640 Cat.# Por to withstandhighpressuredifferentials,butdialysisisslowerthaneitherSpectra/ Spectra/Por Spectra/Por !! ® 2orSpectra/Por ® ® ® ® ® ® 4RegeneratedCellulose(RC)membranesaresimilartoSpectra/Por 5RegeneratedCellulose(RC)membranesarereinforced 4 RCDialysisMembranes-MWCO12-14kDa 6RegeneratedCellulose(RC)membranesarerecommendedfor 6 RCDialysisMembranes 5 RCDialysisMembranes-MWCO12-14kDa 54 815(50) 30(100) 30(100) 30(100) 30 m(ft) 18 6.4 3.3 2.0 0.32 Ready-to-use dialysissacksfor5to400mLsamples,packageof10 48 mL/cm Ready-to-use dialysissacksfor1to40mLsamples,packageof10 29 Flat discs,100mmdiameter, packageof50 20 Flat discs,47mmdiameter, packageof50 16 Flat discs,33mmdiameter, packageof50 6.4 Flat sheets150mmxmm,packageof25 mm 75 45 32 25 10 mm Flat Width 0 42 . 10(33) 10(33) 10 10 10(33) 3.7 10(33) 2.5 10(33) 0.45 10(33) 0.32 10 10(33) 3.7 22 2.5 10(33) 18 1.8 10 7.5 10(33) 1.0 6.4 0.45 10(33) 0.1 22 10 10(33) 18 6.4 34 15 10(33) 28 3.3 11.5 1.8 12 10 7.5 10(33) 10 3.8 1.0 0.45 34 10(33) 0.1 29 10 28 50k 10(33) 20.4 6.4 24 50k 10(33) 15 18 50k 3.3 11.5 1.8 10 12 50k 7.5 10 6 3.8 1.0 10(33) 25k 0.45 45 25k 0.1 29 10(33) 32 25k 8.0 20.4 24 25k 10(33) 15 10 5.1 18 25k 11.5 10 3.3 12 25k 1.8 7.5 10 6 3.8 15k 1.0 10 45 15k 32 10(33) 0.45y 32 25.5 15k 0.1 10(33) 24 20.4 15k 9.3 18 10(33) 15k 15 6.4 10(33) 12 15k 11.5 10(33) 6 1.0 10k 7.5 6.4 50 3.8 10(33) 10k 4.6 40 10k 32 34 1.0 10k 24 29 6.4 10k 18 11.5 4.6 10k 29 12 1.0 8k 24 6 8k 11.5 8k 54 29 8k 45 24 8k 18 11.5 45 8k 38 8k 18 3.5k 45 3.5k 38 3.5k 18 2k 2k 2k 1k 1k 1k MWCO mm Lc m(ft) mL/cm mm mm 4 96 15(50) 15(50) 62 13 89 41 Flat sheets275mmxmm,packageof25 140 65 Flat Width ® 4. imtrV Diameter mm Lc Lengthm(ft) mL/cm mm mm Flat Width imtrV Diameter ® 6 Tubings 6 : imtrV Diameter olume/Length olume/Length olume/Length Package Length Package Length (100) Package (33) (33) (33) (33) (33) (33) (33) (33) (33) (33) (33) (33) (33) ® 2, Proteomics B.107 (16) (16) (16) (16) (16) (16) (16) (16) (16) (16) (16) (16) Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting Package Lengthm (ft) olume/Length ! Separation techniques (Proteins) techniques Separation Diameter V Flat Width mm rmm mL/cm e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] 50k 34 22 3.7 5 (16) 15k15k15k15k25k25k 1825k 2425k 3225k 4525k 650k 12 11.550k 18 15 24 20.4 28 29 34 1.0 12 3.8 7.5 28 1.8 11.5 3.3 15 6.4 18 22 0.1 5 (16) 0.45 7.5 1.0 18 5 (16) 5 1.8 2.5 5 (16) 3.7 5 (16) 0.45 5 5 (16) 2.5 5 (16) 5 (16) 5 (16) 5 5 (16) 10k10k10k15k15k 24 32 45 6 12 15 20.4 29 3.8 7.5 1.8 3.3 6.4 0.1 0.45 5 (16) 5 5 (16) 5 (16) 5 8k8k8k8k 68k 1210k10k 2410k 32 50 3.8 7.5 6 12 15 18 20.4 0.1 32 0.45 3.8 7.5 1.8 3.3 11.5 5 (16) 8.0 5 0.1 0.45 1.0 5 (16) 5 5 (16) 5 (16) 5 5 (16) 2k2k3.5k3.5k 38 45 18 45 24 29 11.5 29 4.6 6.4 1.0 6.4 5 (16) 5 (16) 5 5 MWCO 1k1k1k2k 18 38 453.5k 18 11.58k 24 29 54 11.58k 1.0 4.6 18 6.4 1.0 34 40 5 (16) 5 (16) 11.5 5 (16) 5 (16) 9.3 25.5 1.0 5 5.1 5 (16) 5 7 Regenerated Cellulose (RC) membranes are recommended for are recommended membranes Cellulose (RC) 7 Regenerated 7 RC Dialysis Membranes 7 RC Dialysis 7 Tubings : 7 Tubings ® ® ® Spectra/Por applications where impurities (metal, disulfides) may be crucial (i.e. before MS analysis, may be crucial disulfides) (metal, where impurities applications alternative choice. membranes are an Biotech activities, crystallizations…). for enzyme Spectra/Por O30660 128524 O30650 607910 317290 O30630 128506 O30640 128518 412560 340830 128458 O30620 679180 756710 756720 128456 O30610 128406 128418 847770 O30590 O30670 BI1900 128356 O30580 O30560 O30570 728110 130080 O30530 O30550 Cat.# 714160 O30540 920160 128358 O30600 Spectra/Por B.108 Proteomics Separation techniques(Proteins) 100k 50k 25k 10k 8k 3.5k 2k 1k 500 2 mL V 100 mm 8 (Da) MWCO Sterile BiotechCEcatalognumbers 100k 50k 25k 15k 10k 8k 5k 3.5k 5 mm 2k mm 1k 8 500 100 (Da) MWCO Biotech CEcatalognumbers Desalting /Dialysis,Ultrafiltration 310O05 310O07 O30180 O30130 O30080 O29980 O29930 O29830 O29780 O30170 O29730 O30120 O29680 O29630 O30070 O29970 O29920 O29820 O29770 O30160 O29720 O30110 O29670 O29620 O30060 O29960 O29910 O29810 O29760 O30150 O29710 O30100 O29660 O29610 O30050 O29950 O29900 O29800 O29750 O30140 O29700 O30090 O29650 7.5 O30040 O29600 O29940 O29890 O29790 6.4mmDiameter O29740 O29690 O29640 O29590 5 mmDiameter ltWdh1 mFa it 2m ltWdh1 mFa it 24mmFlatWidth 16mmFlatWidth 12mmFlatWidth 10mmFlat Width Flat Width olume 4 mL Volume 5 mL Volume 10 mL Volume 20 mL 20 Volume mL 10 Volume 5mL Volume 4mL Volume olume Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 840466 850390 040332760 661070 203440 O30330 192600 557000 329200 348120 988440 986620 190540 660010 960260 181590 962470 963060 189670 171840 406860 824520 O30320 560020 834720 445190 187880 342700 556950 683250 683460 O30310 210900 O30400 O30260 965380 O30380 187540 O30340 352620 3.1mL/cmlength 875440 851370 404250 1.8mL/cmlength 824100 881700 821950 181150 0.81mL/cmlength 828430 871000 0.45mL/cmlength 828410 0.32mL/cmlength 824090 0.20 mL/cmlength ltWdh1 mFa it 2m ltWdh1 mFa it 4m ltWdh31mmFlatWidth 24mmFlatWidth 16mmFlatWidth 12mmFlatWidth 10mmFlatWidth Flat Width imtr64m imtr75m imtr1 mDaee 5m imtr20mmDiameter 15mmDiameter 10mmDiameter 7.5mmDiameter 6.4mmDiameter Diameter CE Biotechmembranesaresuppliedwetin1%NaN length (33ft). Sterile BiotechCEMembranes These state-of-art Spectra/Por length (33ft). CE Biotechmembranesaresuppliedwetin1%NaN water. membrane shouldnotbeexposedtoorganicsolventsatconcentrationsexceeding5%in and isopropanol)withoutapparentdamage,butwithpossiblealterationofMWCO.The membrane. Thismembranewilltolerateexposuretoloweralcohols(methanol,ethanol, solvents. Strong polarsolvents suchasacetone,MEK or dioxanewilldamagethe sample recovery. The Spectra/Por with anextremelyhighpurityandfeaturesabiologicallyinertsurfacetoyieldoptimum macromolecules. TheadvancedmanufacturingprocessprovidesCEdialysismembranes Molecular WeightCutOff(MWCO)selectivityforseparationofionicspeciesaswell Spectra/Por !! ! ! ! ! ! ! ! ! 25 pre-cutmembranetubing,14cmlengthsforusewithSpectra/Por Irradiated membrane,individuallypackagedindeionizedwater 2, 4,5,10,and20mLsamplevolumesizes 6 diametersavailable(5forsterilemembranes) No specialchemicalpre-cleaningrequired Hydrophilic anduniformmolecularporousdialysismembranes Fourteen preciseMWCOsrangingfrom100Dato1000K An UltraHighPurityandPreciseSeparationDialysisMembrane 320O08 320O30300 959760 967070 O30290 822510 O30250 181480 O30280 835200 32450 816870 O30270 O30240 ® BiotechCEDialysisMembranes mDaee 0m imtr15mmDiameter 10mmDiameter mm Diameter 330O06 O30370 O30360 O30350 O30230 824530 ® CelluloseEster(CE)dialysismembranesoffer anexcellent ® BiotechCEmembranesaresensitivetoorganic 340560030 O30390 O30410 962480 3 3 preservative,andin10m preservative,andin10m 691350 ® Closures 50k 25k 100k 15k 10k 8k 5k 3.5k 2k 1k 500 100 MWCO MWCO 100k 50k 25k 10k 8k 3.5k 2k 1k 500 100 Proteomics B.109 Related products : CelluSep T1.2.3. Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting ! preservative, in 10 m length 3 Separation techniques (Proteins) techniques Separation O29410 989290 923340 e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] closures 2, 5, 10, and 20 mL sample volume sizes. ® Flat WidthFlat 12 mm Flat Width 19 mm Flat Width 25 mm Flat Width flat width 12 mm flat width 16 mm flat width 24mm flat width Flat Width 8 mm Flat Width 10 mm Flat Width 16 mm Flat Width type F (PVDF) Dialysis Membranes Biotech RC Dialysis Membranes Biotech RC Type F Dialysis Membranes are recommended when a wide compatibility Type Biotech Regenerated Cellulose (RC) dialysis membranes are designed Cellulose (RC) dialysis membranes Biotech Regenerated Biotech Regenerated Cellulose (RC) dialysis membranes are available Cellulose (RC) dialysis membranes Biotech Regenerated ® ® membranes are supplied wet in 0.1% NaN ® ® ® ® O33080U35530 O30480 U35510 O33100 U35570 O33090 U35550 U35520 U35500 U35560 U35540 5 mm diameter0.2 mL/cm lenght 7.5 mm diameter 0.45 mL/cm lenght 0.81 mL/cm lenght 10 mm diameter 1.8 mL/cm lenght 15 mm diameter O29380 O29400O29460O29520 909510 O29470 O29530 O29390 O29480 O29540 985570 829870 3.8 mm Diameter0.1 mL/cm lengthO32500 5.7 mm Diameter27750 0.25 mL/cm lengthO29420 7.5 mm Diameter 0.45 mL/cm length O32510 14 mm Diameter 1.5 mL/cm length O31540 O325202 mL volume 623250 5 mL volume O32530 624660 10 mL volume 20 mL volume O29360 O29370 O29350 923100 Regenerated Cellulose (RC) dialysis Membrane Tubing dialysis Membrane Cellulose (RC) Regenerated purity Ultra high precleaning required No special Precise MWCO ! ! ! ! Spectra/Por 1000 k 500 k 250 k (33ft). They should be refrigerated and not allowed to dry out. They should be refrigerated (33ft). MWCO 8 mm Spectra/Por 8k 15k 25k (pre-cutted) Biotech RC Dialysis Tubings Sterile water 25 pre-cut membrane tubing 14 cm lengths for Irradiated membrane, individually packaged in deionized use with Spectra/Por MWCO mm 8 3.5k 8k 15k MWCO 4 mm 10k Spectra/Por SpectraPor is desired with various aqueous acids or bases and organic solvents, or resistance to heat. Chemical compatibility is available on inquire. They can be steam autoclaved protein without affecting their performances, and can be heat sealed. However adsorption is higher. Biotech RC Dialysis Tubings with a strictly controlled MWCO and offer exceptional freedom from impurities such as MWCO and offer exceptional freedom with a strictly controlled found in standard dialysis contaminants which are commonly sulfide and metal required for Biotech RC are no special pre-cleaning treatments membranes. There Membranes. glycerol as a humectant in 15 m (soft) rolls. Supplied as dry membrane tubings with Spectra/Por in a variety of style for different applications. Rolls of dialysis tubing are available for applications. Rolls for different in a variety of style are also available. applications. Irradiated tubing pieces most general dialysis Spectra/Por B.110 Proteomics Separation techniques(Proteins) e C25000 15000 RC 000 10 000 8 RC 500 3 RC Red RC RC Orange Yellow 50000 25000 CE Natural Black CE 15000 CE 000 10 Spectra/Por Blue 8 000 5 CE 000 CE Green 500 3 Red CE CE Orange CE 1 000 Yellow 2 000 500 Natural CE Pink CE 100 CE Black Dark Green Violet CE Amber Light Blue Spectra/Por references(10unitsperbox) Catalog Versatile : Large choiceofsizes,cut-offsandmembranetypes: Excellent andeasyrecovery: Rapid dialysis: Very easytouseandsure: Ultra-convenient uniquefloatabledialysisdeviceforsamplesfrom0.3to10mL Float-A-Lyzer (Ready-to-use/small volume/Electro-elution) Specialized devicesDialysis a b Cut-off Mbr color Cap Desalting /Dialysis,Ultrafiltration Diameter 5 mm 5 mm 5 mm 10 mm 10 mm 10 mm 10 cm 16 mm 10 cm 10 mm 10 9 cm 5 mm 9 cm 5 mm 8 cm 5 mm 7 cm (cap diametre:36mm) Diameter Totallength Volume Dimensions ! ! ! ! ! ! Replaced accesstosample,concentrationispossible Exists in6volumes,13cutoffs,2membranequalities Open, pipetwiththeprovidedpipette Optimal sample/membrane/buffer contact No riskofleakage,orpuncturingwithsyringeneedles No wash,nonods,accessorytoloadorfloat ® ® Float-A-LyserwithBiotechRegeneratedCellulosemembranes Float-A-LyserwithBiotechCelluloseEstermembranes yeMC 0 L50µ 1 500µL 300µL MWCO type ® Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 100 000 860846 860841 870884760 884590 884720 884530 884580 884460 884710 884510 884340 884570 884450 884670 884500 884330 884560 884440 884190 884660 884490 884310 884090 884550 884180 884430 884020 884480 884640 884080 884300 883950 884420 884160 884540 884010 874530 884290 884070 884470 883940 884150 884000 884390 874510 883880 884050 883930 884280 883780 883720 884140 883870 883990 874490 884040 883770 883920 883710 883330 883860 884100 883980 874470 883240 883760 884030 883910 883320 883700 883830 883600 883960 874300 883230 883750 883500 883310 883900 883820 883690 883590 883210 874280 883490 883740 883290 883570 883800 883680 883180 883480 883730 883260 883530 883670 883170 883470 883250 O34290 883160 883460 883510 883450 Max. samplevolume 300 µL 500 µL 1 mL 3 mL 5 mL 10 mL 10 5 mL 3 mL 1 mL µL 500 µL 300 !! mL 3 mL 5 mL 10 mL 10 5mL 3mL mL or otherreadytousedevices. performing thanyoudowithconventionaltubings que devicesmakingthedialysiseasierandmore All thesefeaturestogether, Float-A-Lyzer areuni- trifugation inatube. maximal recoverymaybeobtainedbyashortcen- sample, usetheprovidedpipette. Alternatively, cap, andpipette.Now, whenitistimetorecoverthe never knewwithotherdialysissystems:openthe rapid test,ortoaddareagent,that’ssimpleasyou check fordialysiscompleteness,ortoperforma or supports!Whenyouneedtotakeanaliquot together: youdonotneedsyringes,floats,weighing cap andputinabuffertank,aloneorseveral the sampleinsideasyoudoinanytube,close bel yoursampleonthecap.Opencap,pipet thus ready-to-use.RinsebrieflytheRCunits.La- Float-A-Lyzer arepackagedindividually, wetand your Float-A-Lyzer: Then, let’sappreciate #292600 page115). changing ofrecipient!(SeeSpectraGel convenient concentrationstep they canbe or purificationpolishingaremadeeasy, while reactions, bufferexchangebeforeanalysis, Desalting proteinornucleicacidslabeling applications ofthelab. 10Kda with500µland3mlallowcoveringmost closest toyoursample.Forexample,theMWCO choice inthemarket!Thenchoosevolume range from100Daltonsto000,thegreatest removed andkept.MWCOareveryprecise, regarding themolecularweightofmoleculestobe (MWCO), codedbyacolorforconvenience, Now, choosethe result isexcellenceindialysisefficiencyandrate. the bufferisagitatecorrectly(magneticstirrer), face tubing whileits to move, The tubevolume,withoptimalproportions,isfree contaminants arecritical. preferred whenheavymetalsorsulfide PVDF membranes). higher resistancetosolvents(up5%. Above see applications, providingminimalproteinadsorption, Cellulose 2 membranetypesareavailable: Just brieflyrinse. preservative. Donotboilorsoakpriortouse. individually packagedwetin.1%sodiumazide devices. Float-A-LyzerDialysisTubeis that arenoticeableincompetitordialysis lower tominimalthecontentincontaminants RC orCE),toavoidtheneedwash,and The use. dialysis efficiency, aswellconvenienceof Float-A-Lyzer devicesadesignedtooptimize Technical tip istotallysweptbybuffermovements.Provided membrane quality maximizing samplemixing ester(RC)isrecommendedformost membrane exchangelargesur- combined toafastand Molecular WeightCut-Off Acetate Cellulose how simpletouse isstateofart(Biotech Regenerated insidethe without maybe is Proteomics B.111 Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting ! 2 mL2 500 µL420940 mL 1 mL 2 mL 5 Separation techniques (Proteins) techniques Separation 15kD 25kD Length Length Exposed MembraneDialyzer Total 2.0 cm 6.0 cm 58370 mm 4.5 cm cm 8.5 Membrane 820240O32720O32730O32740 374710568100 213240 O32790 914490 438170 404880 987520 957650 219280 219360 759770 O32650 O32660 759800 654110 O32550 759810 O32570 O32580 O32600 O32610 O32620 Cellulose Ester MembranesSample Volume 820190820200820220O32680 408300O32690 974620820230 168610 Regenerated Cellulose Membranes O32710 O32760 O32800 342890 987330 168640 O32780 222680 322940 974830 O32640 673220 673230 673390 e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] ® Volume Diameter Maximum Volume 761920761850 761930 761900 762000 761910 905300 905320 Molecular Weight Cut-Off 3.5KD905120 8kD 874540 905280 905290 ordering info (12 units per box) ® ® Dispodialyzer are sterile disposable dialysis devices. are sterile disposable Dispodialyzer ® Wide Selection of MWCO : 100 to 100 000 Da Wide Selection of MWCO Color coded caps to 5 mL : 500 µL Sample Volumes upright without pontoons Dispodialyzers float and unloading are included Pipettes for loading individually pouched Irradiated (sterile) and ! ! ! ! ! ! 1 mL1 mL2 mL5 1.5 mL 2.2 mL 6.5 mL 5 5 mm 5 mm 8.5 cm 8.5 cm cm 13 cm 12 Dispodialyzer dimensions Sample Volume 500 µL 600 µL 5 mm 50 µL 100 µL MicroDispodialyzer 10 µL Total Length : 2.9 cm Total Cap Diameter : 3.6 cm Volume The membrane, made of Biotech Regenerated Cellulose, forms a well glued to the upper The membrane, made of Biotech Regenerated Cellulose, forms a well glued to the volume, plastic holder/floater/cap, which maximizes the membrane surface to sample and thus the dialysis rate. MicroDispodialyzer but This device is the micro-brother of the Float-A-Lyzer : as convenient and performing, for dialyzing micro-volumes of 100 down to 10 µL ! OrangeBlue 15k 100k YellowRedGreen 10k 25k 50k Dark GreenBlack 2k Clear 3.5k 8k Light BlueAmber 100 500 Dispodialyzers : ordering info (10 units per box) Packaged 10 per box CapColorViolet MWCO Pink 1k 500 µL 5k mL 1 Spectra/Por but comes as sterile devices, the features of Float-A-Lyzer shares most of Dispodialyzer water). in distilled sterile preservatives (wet units without Dispodialyzer Just open the easy-to-use dialysis tube, rinse, load your sample and screw the leak- dialysis tube, rinse, load your Just open the easy-to-use floats will ensure the Dispodialyzer head space Allowing a small proof cap in place. completely eliminate fully immersed during dialysis. Dispodialyzer vertically and remains knot tying and tubing closures. the need for membrane preparation, B.112 Proteomics Separation techniques(Proteins) Desalting /Dialysis,Ultrafiltration Related products:PBS(PowderPack,or Tabs) beaker, dependingonthesizeofbeaker. Note :MultipleFastDialyzercanbeusedinthesame 1 4 3 2 Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 during dialysis. that helptomixthesample should containabubble the model.Thechamber maximum dependingon between 50µlandthe Sample volumemustbe the bottomwel 1. Apply thesamplein Fill wellwithsample micropipette thoughatip. membrane), ora needle (puncturethe with asyringethrough removed byaspiration The samplecanbe 3. Recoverthesample and aspiratesample Perforate membrane sample typeandsize. more dependingonthe at least30min(0.1µl)or Allow thedialysistooccur under constantagitation. Put thedeviceinbuffer 3. Dialysis Stir &tumble brane top parttofixthemem- chamber, andpushthe membrane onthebottom Set a2.5xcm2square 2. Assembly Push toassemble while bigmoleculesofinterest areretainedinsidetheGeBAflexchamber. hemipermeable membraneallows undesiredsmallmoleculestoberemovedfromsample, is placedinadialysistankor an electrophoresischamber. That’s easy! The highquality acid, extract,reactionmixtureorelectrophoresisgelpiece)is pipettedintothedevicethat dialysis ofsmall-volumesampleaslow50µlupto3ml.The sample(proteinornucleic Protein, RNA,DNAandtheircomplexesfromanygelmatrix, andforconvenientrapid GeBAflex-tube isbasedonasingleoneusedeppendorf-like tube,forrapidextractionof rinse indistilledwaterisrecommendedbeforeuse. 1 ml,0.5and0.1mlFastDialyzers(eachsquaremaybecutin4pieces).Abrief FastDialyzers. Onesquaresuitsto5mlFastDialyzer(1dialysisprocedure),and FastDialyzer areconvenientre-usabledialysisunits: FastDialyzer™ :dialysis&desalting The GeBAflexgenerationtechnologycombineshighperformance in: 2 applicationsinonedevice! GeBaFlex DialysisandElectro-elutionsystem 55 mmx55 55mmx F1 Membrane,3.5KMWCO 55mm x55mm F2 Membrane,6-8KMWCO F3 Membrane,12-14KMWCO Note :OnealsomayusedCelluSepdialysistubings25-50mmflatwidth)thatcanbecutadequately. CelluSep detergents, andwithUltrasoniccleaners. Qty FastDialyzers units canbecleanedbyglasswarewashingmachinesandlaboratory (1 uniteachof0.1ml,0.5mland1ml) Mix ofFastDialyzers Cat.# FastDialyzer 5ml FastDialyzer 1ml FastDialyzer 0.5ml FastDialyzer 0.1ml Designation CelluSep !! Technical tip ! ! ! ! ! ! ! need frameorjointstofit)makingelectrophoresisgelpiecessoeasyandefficient. are themostconvenientdialysisdevicessincetheyready-to-useinstandardelectrophoresissystem(donot Most dialysismembranesdescribedabovecanbeusedinseveralelectro-dialysissystems.Now, GebaFlexmodules separating electrolytefromcationsandanions,oranions. driving force.Applicationsincluderemovingsaltsfromwaterorproteinaqueoussolutions,butalso Electro-Dialysis Available inarangeofconvenientvolumes:0.1mL,0.51.0mLand5mL. Reusable andrugged,whichreducescostminimizesbio-hazardouswaste. efficient, andhelpspreventsampleloss. Easy touse: The FastDialyzermethodofloadingandunloadingsample iseasy, with aFastDialyzer. You maychooseyourownmembranes(andMWCO)"rightoff theshelf"touse buffer exchange. work fast:mostequilibrationtimesaverage1hourwithsimpledialysisofsaltsor Dialysis ofsmall-volumesamples. Electro-elution ofbiologicmacromoleculesfrompolyacrylamide oragarosegels ® ® membranesareavailablepre-cuttedin55xmmsquaresforusingwith MembraneSquares isthetransportofionsthroughasemi-permeablemembraneasresultanelectrical FD1000-3 FD500-3 FD100-3 AA9890 FD1000-1 FD500-1 FD100-1 FD999-3 3 u 3 u 3 u 1 u 1 u 1 u 1 u 1 u 10/pk 10/pk 10/pk BA0911 BA0901 BA0891 Proteomics B.113 Nucleic acids 3 volumes (50 µl to 3 mL) 3 volumes (loading and unloading) Easy to use cleaning or autoclaving no need for needed no additional accessories ! ! ! ! Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting RNA or DNA > 95%. ! Proteins Separation techniques (Proteins) techniques Separation small-volume sample as low as 50-800 µL small-volume sample as low as 50-800 High Recovery : > 97% Easy to use : no need accessories, quick Convenient loading and un bading of sample Repeated access to sample Cost effective 3 MWCOs Biology grade Molecular Cost effective Scalable / High Throughput Suits low starting material : 1 to 25 µg of proteins and nucleic acids. Suits low starting material : 1 to 25 µg of High Recovery : Protein > 90% No residual salts (suits MALDI-MS)... ElectroElution : compatible with any horizontal electrophoresis system Suits DNA and oligonucleotides >20 bp, RNA, Proteins >3500 MW Dialysis membranes are made of ultra-clean Regenerated Cellulose to (6-8000 and 12-14000 MWCO), or cellulose ester (3 500 MWCO) : there is no need they are wash them, even for most demanding applications (i.e. cristallography) because sulfure and metal free. tray and GeBAflex-tubes are provided with floating rack (Dialysis kits), or with supporting floating rack (Extraction kits) sizes GeBAflex-tubes suits for volumes ranging from 50 µl to 3 ml. 3 dialysis chamber cap) are available : MINI [10-250 µl], MIDI [50-800 µl], MAXI [100 µl-1 ml (with an adaptator or 2-3 ml (standard cap)] ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Applications DIALYSIS Desalting , Buffer exchange,... Benefits : General Benefits : General Benefits EXTRACTION Concentration... ElectroElution, Molecular Size exclusion, Benefits : Product characteristics : e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] B.114 Proteomics Separation techniques(Proteins) Desalting /Dialysis,Ultrafiltration µL. dialysis volumebetween 100-2000and100-3000 are providedwiththekit)onecaneasily adjust the § BychangingthemaxiGeBAflex-tubecap (twocaps 800MWCO, 30tubes) (MIDI, 3500MWCO,30tubes)and#U2708D (MIDI,6- packaging (withoutboxandfloatingrack): #U2707D Items U2707BandU2708Bareavailableas economic * Includefloatingrack,handbook. Scale upsystemforHTS. volume between100-2000and100-3µL. are providedwiththekit)onecaneasilyadjustdialysis § BychangingthemaxiGeBAflex-tubecap(twocaps * Includebuffers,supportingtray, floatingrack,handbook Protein recoveryfromgelslices GeBAflex yieldssuperiorrecoveryinDNAor Technical tip Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 electrophoresis unit! of 24)areextractedsimultaneously, typicallyin80min/50kDaproteins,one tanks. Highthroughputelectroelutionispossible:forexample,24samples(ormultiples assembled onatraytosupportit(them)adequatelyinstandardelectrophoresishorizontal 10-250 µlsampleor0.4x1.1cmgelslice(miniGeBA).TheGeBAflextube(s)canbe samples (i.e.includingparticles)withGeBAflextubes.Thedialysischamberaccommodates MacroBiomolecules canberapidlyextractedfromelectrophoresisgels,aswellcomplex GeBAflex-tube kitsforEXTRACTIONbyElectroelution Applications :upanddownstreamapplicationsofdialysiswithGeBAflexinclude GeBaflex tubesinondialysistank. recovered withhighyield.HighThroughputdialysisispossible,usingtraystosupport and desaltingofsmallsamplesvolume.MacroBiomoleculesarerapidlydialyzed GeBAflex-tube provideseasytohandleandperformingsystemfordialysis,bufferexchange GeBAflex-tube kits forDIALYSIS 12 000-14000 GeBAflex-tubeExtractionkits* 6 000-8000 3 500 MWCO Downstream applicationsofelectroelutionwithGeBAflexfromelectrophoresisgelsinclude: MS Buffer MS Buffer Extraction Buffer(Kac) Precipitation Buffer(TCA) Floating rackMidi(for1-4GeBAflex-tubes) Supporting trayMidi(for1-4GeBAflex-tubes) Accessories 12 000-14 GeBAflex-tube Dialysiskits* 6 000-8 3 500 MWCO Removal ofethidiumbromidefromnucleicacidsamples Labeling Desalting DNA orRNA Recombinant proteins,proteinsextracts(SDSremoval) Removal ofureafromrecombinantproteins Buffer exchangebeforebiochemistry Desalting ofmodifiedproteins(i.e.aminoterminide-blockage,enzymecleavage,Biotinylation,labeling,conjugations) Proteins Removal ofRNAcontaminationfrominvitrotranscription Purification ofRNAtranscripts RNA structuremapping DNA orRNA Recover proteinsrunonisoelectricfocusinggelsandsimultaneouslyremoveampholytes Recovery ofgel-purifiedproteinsforimmunizationlaboratoryanimals Purification ofverysmallProteinquantities Peptide mapping HPLC analysisforpeptidesequencing Mass spectrometryanalysis(MWdetermination,structuralcharacterizationandidentification) Proteins Protein-nucleic acidscomplexesformationandanalysis Radioactive andfluorescentsequencing !! Applications Applications AZ390B AZ389B MAXI ( 100-3000 µL ) AZ3901 AZ3900 AZ3891 ) µL 50-800 ( MIDI AZ3890 MINI (10-250µL) II(1-5 L)MD 080µ MAXI ( 100-3000 µL ) AZ390A MIDI (50-800µL) AZ389A U2707A MINI (10-250µL) 30 u 10 u 30 u 10 u 10 u 30 u 10 u 30 u 10 u U27072 U27070 U27083 U27080 U2708B * U2708A U2707B * AA740A BI2927 BI2926 BI2943 BI2941 BI2931 BI2921 20 u 10 u 30 u 10 u 30 u 10 u 30 u 5 u 5 mL 20 x3mL 20 x3mL 20 x21mL 20 units 20 units AA7401 AA7400 AA7421 AA7420 AA742B AA742A AA740B AA741B AA741A AA7411 AA7410 15 u 5 u 15 u 5 u 15 u 5 u 15 u 5 u 15 u 5 u 15 u § § Proteomics B.115 Cleaning procedure : 1/ wash with cleaning reagent diluted 1:9 with water 2/ 5min soaking 3/wash with water for several minutes Note : Trace elements found in Spectra/Por® RC series 1 through 6 are (ppm) : Calcium < 0.02, Chromium 0.1–0.2, Copper 0.8–1.2, Iron 20–60, Lead 2.0–6.0, Magnesium 0.1–0.3, Nickel 1.3–1.7, and Zinc 1.5–5.0. Note : For ease of use, we recommend Spectra/Por® 7 membranes, which are already treated to remove the membranes which are made contaminants, or BioTech from chemically pure cellulose, or type F (PVDF) mem- brane which are devoided of sulfides and heavy metals. (1:2 in water) A Cleaning procedure : 1/ wash with solution for 1min at 80°C, 2/ wash in water for 2 min at 60°C, 3/ wash with solution B (4 mL/100 mL water). of fluid). Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting 3 ! Separation techniques (Proteins) techniques Separation 250 ml 1 kit 500 g 905700 292600 26530R Microdialysis Hollow Fibers, MWCO 13 kDa Microdialysis Hollow Fibers, 20 u vivo in vivo Microdialysis Hollow Fibers, MWCO 18 kDa in vivo Microdialysis Hollow 20 u in ® ® e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] Heavy Metals Cleaning solution can be used to remove the heavy metal Heavy Metals ® Spectra/Por Spectra/Por in vivo Microdialysis Absorbent series 1 through 6 contain about 0.1% sulfur remaining from the series 1 through 6 contain ® ® ® In vivo delivery (i.e. localized delivery in brain for the evaluation of neuropeptides in brain for the (i.e. localized delivery In vivo delivery transmitters) in combination (i.e. by using radioisotope techniques Metabolic experiments with analytical chemistry) within the extracellular space Analysis of compounds Highly efficient concentration Easy to use, quick No additional recipient or step Do not stick to dialysis bag and is thus easier to handle, greatly facilitating the Do not stick to dialysis bag and is thus easier interfere with following analysis (MS, Do not contaminate the sample, that may with low molecular weight compounds. Crystallography,...) ! ! ! ! ! ! ! sample recovery from dialysis bag, ! manufacturing process. This may be removed if its presence might interfere with the manufacturing process. This may be removed if its presence might interfere sulfide removal kit allows to clean several A subsequent use of the dialysate or retentate. hundred feets of tubing. contamination found in traditional regenerated cellulose membranes. This chelating wash contamination found in traditional regenerated cellulose membranes. This chelating with can be used to remove these heavy metals in situations where they would interfere with later analysis of the dialysate or the retentate, especially where they would interfere kit allows to clean several hundred feets of tubing. cleaning A protein function. Perform in vivo localized recovery or dispensing micro quantities (down cubic mm quantities (down or dispensing micro vivo localized recovery Perform in (solutions A and B) A (solutions ProductSulfide Removal reagent Cat.# Qty ProductHeavy Metals Cleaning reagent Sulfide Removal Spectra/Por Cat.# Qty Heavy Metals Cleaning The Spectra/Por ProductSpectra/Gel Absorbent Cat.# Qty Accessory products for Dialysis Accessory products Membrane cleaning) (Concentration & dialysis, Spectra-Gel O30860 964120 Cat.# Product Qty Applications : Applications Spectra/Por Concentrate easily your dialyzed samples ! Concentrate easily your dialyzed samples is often a hassle, loose time and material, Concentration, when required after dialysis, with proteins), or precipitation (i.e. with DNAs). whatever it is performed by ultrafiltration (i.e. alternative ! a convenient and efficient offers SpectraGel Now, This gel, based on polyacrylate/polyalcohol, absorbs water through the dialysis membrane, This gel, based on polyacrylate/polyalcohol, the sample in its dialysis bag. It is compatible up to 1000 times its weight, concentrating MWCO 15Kda, cut off is. Used with FloatA-Lyzer with all dialysis membranes, whatever the within 90 min with 10 g of SpectraGel. to 0.5 mL is reduced a volume of 5mL over PEG based alternatives : several advantages offers SpectraGel SpectraGel also compares favorably with ultrafiltration (no pressure of centrifuge needed, favorably with also compares SpectraGel time, no additional recipient and transfer improving sample recovery) or precipitation (gain no additional step, better yield,...). B.116 Proteomics Separation techniques(Proteins) Spectra/Por For RapidDialysisofMicro-Volume Samples. notched universalhangerthatfitsovertheedgeofcommonvessels. Open snapcap,fillwithsample,andhangincontainer. Funnelsaresupportedbyathree- end ontothepolypropylenefunnelandsecurewithsiliconeloopfastener. an optimaldialysis.Cuttubularmembranetolengthandclampatoneend,sliptheopen The clampsaremadeofresistantandinertpolyamide6.6(NYLON),donotfloat,for risk ofloosingsample. until theuseropensit.Theyavoidhassleofmakingandcheckingknots,reducing These clampssealthetubingbyasimplepressureandremainlockedduringdialysis hmeVlm m)3 23 112 112 34 96 24 34 560 150 32 16 24 34 230 150 30 10 65 32 620 500 10 24 ChamberVolume (mL) 230 Dialyzer Dimensions LxWxH(cm) 150 Reservoir Volume (mL) Mbr Surf. Area / Minimum SampleVolume (µL) Maximum SampleVolume (µL) Nominal SampleVolume (µL) Number ofSamples Cat.# MicroDialyzers inMWCOrangingfrom100DatoKDa. membrane. Framedmembranesareavailabletoaccommodate allsizesof MicroDialyzers worksbyseparationofthesampletobedialyzed byasemi-permeable Similar toconventionalmethods,the Spectra/Porkey featuresofthesesystems. maximize samplerecovery. Speed andprecisecontrolofthedialysisprocessare applications from24to620µL.Eachunitisdesignedincrease dialysisefficiencyand dialysis methodsofmicrovolumes. They areexcellentforsimultaneous microsample Spectra/Por Micro Dialysissystems 966180 O30510 Cat.# Dialysis Reservoir Silicone loops For 10mmand19flatwidths.Packageoffivefunnelstwosiliconeloops Mini fillerfunnel For 23mm,253240mmand45flatwidths.Packageoffivefunnelstwosiliconeloops Macro fillerfunnels Description Filler funnels Cellu.Sep Cellu.Sep Cellu.Sep Description -maxmembranewidth Tubing standard closures:cellu.Sep 822500 822480 821970 804330 Cat.# wcomposed witharegular, non-weightedClosure Magnetic WeightedClosures Closures forTubings Desalting /Dialysis,Ultrafiltration ! ! ! Not forusewithCEorPVDFtubing continuous motionofthedialysistubing with amagneticstirplate.Noadditionalbarisrequiredinordertomaintain A slowspinmovementofthedialysistubingoccurswhenusedinconjunction Complete submersionofthedialysistubingintobufferchamber ® ® ® Lockingclamp-105mm Lcigcap-6 m90 mm 65 mm Lockingclamp-65mm Lockingclamp-46mm ecito o m)Ht Vol(mL) Dialysis ReservoirwithStirBar Dialysis ReservoirwithStirBar Description ape(qaem)6 52 24 24 65 65 Sample (squaremm) ® ® MicroDialyzersoffer a wide rangeoffeaturestoenhanceconventional MicroDialyzerssystems antcWihe lsrs75 mm 55 mm 35 mm 23 mm Magnetic WeightedClosures Magnetic WeightedClosures Magnetic WeightedClosures Magnetic WeightedClosures Description Tel 33 (0)4 70038855 Hot line 33(0)470 037306 Fax33(0)4 70038260 P58500 Qty P58490 P58480 Cat.# 6.4 x 6.4 x 4.0 12.7 x 6.4 x 4.0 12.7 x 6.4 x 4.0 15.2 x 13.3 x 4.0 x 13.3 x 15.2 12.7 x6.44.0 12.7x6.44.0 4.0 x 6.4 x 6.4 272750 O30920 0x20µ 0x60µ 6x20µ 96x560 µl 16x230µl 10x620µl 10 x230µl Spectra/Por 130 mm Length ® clamps ® Microdialysers 10 u 5 u 5 u ,0 *3 11.5 11.5 12.5 30 1,800 ** 600 * Sealing Width !! a. Qty CB-10100 CB-1070 CB-1050 Cat.# c)Dia(cm) (cm) 390O30960 O30940 10 u 10 u 10 u ® Tubing standardClosures 75 mm. less than80mmandsealingclosurewidth ** accommodatemembranetubingswiththeflatwidthof than 16mmandsealingclosurewidthless75mm. * accommodatemembranetubingswithaflatwidthofless ! ! ! ! ! ! ! Features : Labeled wellsforeasysample identification available forallsystems Pre-cut, framedSpectra/Por Units completelydisassemblyforeasycleaning small membranesurfacearea Negligible samplelossanddilutioneffectsdueto and precisesamplehandling Compatibility withmultichannelpipettorsforquick Easy-to-use snapclampstopreventleakage continuous dialysisbufferexchange Maximum concentrationdifferentialdueto ® membranesare Proteomics B.117 Please inquire for other dialysis systems ! i.e. MacroDialyzer system is equivalent to MicroDialyzer but for larger volumes (1 to 100 ml), suiting especially to equilibrium dialysis Desalting / Dialysis, Ultrafiltration Dialysis, / Desalting Cat.# O30970 O30980 O30990 O31000 O31010 O31020 974800 dialyzers ! Separation techniques (Proteins) techniques Separation and 16 wells dialyzers 150 µL/500 µL MicroDialyzer: 2 Luer Fittings, ® e-mail [email protected] Visit our website : www.interchim.com our Visit e-mail [email protected] O31690O31700O31710O31720O31730O31740O31750 O31820O31760 O31830O31770 O31840O31780 O31850O31790 O31860 O31870 O31880 O31890 O31950 O31900 O31960 O31910 831230 O31920 O31970 O31980 O31990 O32000 O32010 O32020 O32030 O32040 Cat.# dialyzers O31680 O31810 O31940 CE Membrane Frames For MicroDialyzers Frames For CE Membrane ® Desalting of macromolecular solutions Sanitary and multiple micro-dialysis Buffer and pH change Viruses purification after a sucrose gradient Binding studies of fatty acids on proteins or PEG for an approximate Preparation of diluted protein for electrophoresis ten-fold concentration Dialysis of toxic or radioactive samples ! ! ! ! ! ! ! Cellulose Ester (CE) membranes are bound to frames according to the size of the frames according are bound to Ester (CE) membranes Cellulose MicroDialyzers. sodium azide. packed in 1% each being individually frames/package, 10 membranes Gel filtration is an alternative method to dialysis for desalting rapidly samples page B132 (Desalting columns). Gel filtration Ultrafiltration Ultrafiltration uses the same principle as dialysis, but drives by an outer-force Cells). B131 (UF Stirred page 16-Sample Well Plate, 150 µL Sample O-ring (for 500 µL), 20/pkg Applications : Sample O-ring (for 150 µL), 20/pkg Replacement Kit for Spectra/Por Chamber O-rings O-rings, and 2 Dialysis 10 of 500 µL O-rings, 20 of 150 µL Three-way Valves, 2 10-Sample Well Plate, 150 µl 10-Sample Well Plate, 500 µl 96-Sample Well Plate, 150 µL Microdialyser accessories Description 25K 50K 1K 5K 8K 10K 15K 100K 500 2K 3.5K MWCO (Da)100 Use with 10 wells Use with 5, 12, Use with 96 wells Spectra/Por