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Co-Expressed Subunits of Dual Genetic Origin Define a Conserved Supercomplex Mediating Essential Protein Import Into Chloroplasts
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.04.188128. this version posted July 6, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Ramundo et al. Co-expressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts Silvia Ramundo (1,2), Yukari Asakura (3), Patrice A. Salomé (4), Daniela Strenkert (4,5), Morgane Boone (1), Luke C. M. Mackinder (6), Kazuaki Takafuji (7), Emine Dinc (8), Michèle Rahire(8), Michèle Crèvecoeur (8), Leonardo Magneschi (9), Olivier Schaad (10), Michael Hippler (9,11), Martin C. Jonikas (12,2), Sabeeha Merchant (4,5), Masato Nakai (3,13), Jean-David Rochaix (8,13) and Peter Walter (1,2.13) (1) Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, USA (2) Howard Hughes Medical Institute (3) Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka, Japan (4) Department of Chemistry and Biochemistry, UCLA, Los Angeles, USA (5) Present address: QB3, University of California, Berkeley, USA (6) Department of Biology, University of York, UK (7) Graduate School of Medicine, Osaka University, Osaka, Japan (8) Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland (9) Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany (10) Department of Biochemistry, University of Geneva, Geneva, Switzerland (11) Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan (12) Department of Molecular Biology, Princeton University, USA (13) Corresponding authors: [email protected]; Jean- [email protected]; [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.04.188128. -
Plant Biology '99
THE NEWSLETTER OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS Volume 26, Number 1 January/February 1999 Plant Biology '99 III Plant lobs Grab Crabs: Chesapeake Boy Crabs Await You 01 Plant Biology '99! Photo courtesy of Baltimore Areo Convention &Visitors Association. Join your colleagues in celebrating ASPP's SYMPOSIA 75th Anniversary at Plant Biology '99 in Baltimore, Maryland, on July 24-28! Auxin Biology Organizers: Mark Estelle and Ottaline Leyser ASPP's 75th Anniversary Meeting Events: Cell Cycle Regulation Organizer: Tom Jacobs Special President's Symposium functional Plant Genomics Banquet Honoring Past Officers, Award Winners, & Organizer: JeffBennetzen ASPP Traditions Biochemical Genetics Organizer: Dean Della Penna Chesapeake Bay Crab & Chicken Feast President's Symposium: Global Issues in Plant Biology ASPP Family Tree Organizer: Brian Larkins Speakers: Peter Raven, Lester Brown, and Extended Poster Sessions Robert Fraley INSIDE ... i T ASPP-Sponsored Workshop in Greece . ' '~. I' T Novartis, UC-Berkeley Plant Scientists Reach $25 Million Research Collaboration T Call for Nominations for Officers and Awards ASPP Future ASPP Annual Meetings OFFICERS & STAFF .CONTENTS flresident Brian A. Larkins .".""""""" """.. 520·621·9958 1 flresident·Elect .·Plant Biology '99 Deborah Delmer """"""" .."""""""""""""""",,, 530·752·7561 1999 Immediate flost flresident ·3 '" . Ken Keegstra .."""""""""""" ..""""""""""""",517·353·2770 Saturday, July 24, through Secretory . ASPP-Sponsored Workshop in Greece Daniel R. Bush ""......,.."""""""""", ..,..""""""", 217·333·6109 Wednesday, July 28 Treasurer ;MbnagingEditor of Plant Physiology Terri Lomax ."""" .."........."""""""""""."""""", 541·737·5278 .Resigns ' Baltimore, Maryland Choir, Boord of Trustees Douglas D. Randall .........".""""""....."..."""""" 573·882·4847 ASPP's 75th anniversary Choir, flublications Committee 4 . Rebella Chasan .."""""""..."......."""""""""""" 202·628·1500 Public Affairs .' '. meeting Choir, Committee on the -NSF's $85 Millibn in Awards Status of Women in fllont flhysio/ogy Ann M. -
Electron-Transfer Reactivity of Metalloproteins in Folded, Partially Unfolded, and Completely Unfolded Forms Scott Ichm Ael Tremain Iowa State University
Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 2002 Electron-transfer reactivity of metalloproteins in folded, partially unfolded, and completely unfolded forms Scott ichM ael Tremain Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Inorganic Chemistry Commons Recommended Citation Tremain, Scott ichM ael, "Electron-transfer reactivity of metalloproteins in folded, partially unfolded, and completely unfolded forms " (2002). Retrospective Theses and Dissertations. 487. https://lib.dr.iastate.edu/rtd/487 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. -
Synthase of Chlamydomonas Reinhardtii: Import and Cleavage of the Precursor Protein (Chloroplast Coupling Factor 1/Nuclear Encoded/Transcription/Translation) LLOYD M
Proc. Nail. Acad. Sci. USA Vol. 85, pp. 1369-1373, March 5, 1988 Biochemistry Isolation of a cDNA clone for the y subunit of the chloroplast ATP synthase of Chlamydomonas reinhardtii: Import and cleavage of the precursor protein (chloroplast coupling factor 1/nuclear encoded/transcription/translation) LLOYD M. YU*t, SABEEHA MERCHANT*§, STEVEN M. THEG*, AND BRUCE R. SELMAN* *Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706; and tThe Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138 Communicated by Henry Lardy, October 19, 1987 ABSTRACT A cDNA library from Chlamydomonas rein- enzyme (11), and the mechanism of protein import into the hardti, constructed in the phage expression vector Agtll, was chloroplast. probed with antiserum directed against the nuclear-encoded y Chlamydomonas reinhardtii is a genetically malleable subunit of the chloroplast H+-transporting ATP synthase green alga (12) that contains one large chloroplast per cell. It [ATP phosphohydrolase (H+-transporting) or chloroplast is possible to isolate intact chloroplasts (13-15) that can coupling factors 0 and 1, EC 3.6.1.34] of C. reinhardtii. A import precursor proteins (16). Precursors of the nuclear- cDNA was isolated and transcribed in vitro. The transcript was encoded subunits are difficult to detect in vivo since their translated in vitro and immunoprecipitated with anti-y- half-lives are very short (9, 10). The study of the import of subunit serum to yield a product that coelectrophoresed with these subunits and their assembly into the complex is most the immunoprecipitated product from in vitro-translated poly- easily accomplished by using isolated chloroplasts; thus, it adenylylated RNA. -
2013 Photosynthetic Systems Research Meeting
2013 Photosynthetic Systems Research Meeting Westin Annapolis Hotel Annapolis, MD November 3-6, 2013 Office of Basic Energy Sciences Chemical Sciences, Geosciences & Biosciences Division 2013 Photosynthetic Systems Research Meeting Program and Abstracts Westin Annapolis Hotel Annapolis, MD November 3-6, 2013 Chemical Sciences, Geosciences, and Biosciences Division Office of Basic Energy Sciences Office of Science U.S. Department of Energy i Cover art is taken from the public domain and can be found at: http://all-free-download.com/free-photos/leaves_green_back_light_230944.html The research grants and contracts described in this document are, unless specifically labeled otherwise, supported by the U.S. DOE Office of Science, Office of Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division. DISCLAIMER This report is a compilation of accounts of work sponsored by an agency of the United States Government. Neither the United States government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. ii Foreword This volume provides a record of the third biennial meeting of the Principal Investigators (PIs) funded by the Photosynthetic Systems program and is sponsored by the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences (BES) in the U.S. -
Trace Metal Utilization in Chloroplasts
Chapter 10 Trace Metal Utilization in Chloroplasts SSabeehaabeeha S. Merchant Merchant*∗ Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, U.S.A. Summary ............................................................................................................................ 200 I. Introduction ................................................................................................................... 200 A. Transition Metals Function as Redox Catalysts ........................................................... 200 B. Trace Metal Deficiency Impacts the Chloroplast ......................................................... 201 C. Metalloprotein Assembly—Thermodynamics vs. Kinetics ............................................. 201 D. Fe, Cu and Mn ................................................................................................... 201 II. Fe ................................................................................................................................ 202 A. Ferritin .............................................................................................................. 202 B. Heme, FeS and Fe Cofactor Synthesis ..................................................................... 203 1. Heme ....................................................................................................... 203 2. FeS .......................................................................................................... 204 a. Discovery and Function of Prototypical -
The Many Legacies of ASPB by SALLY MACKENZIE University of Nebraska–Lincoln
May/June 2017 • Volume 44, Number 3 p. 4 p. 14 p. 21 ASPB Announces Jenna Gallegos ASPB Welcomes 2017 Award Awarded ASPB/AAAS Winnie Nham as Winners Mass Media New Education Fellowship Coordinator THE NEWSLETTER OF THE AMERICAN SOCIETY OF PLANT BIOLOGISTS President’s Letter The Many Legacies of ASPB BY SALLY MACKENZIE University of Nebraska–Lincoln FEBRUARY 14–17, 2018 t ASPB, so much has If ASPB is to continue its FEBRUARY 14–17, 2018 TUCSON,TUCSON, AZ AZ changed over the leadership role in plant biology, past few years. Our additional financial resources HILTON EL CONQUISTADOR RESORT A HILT ON EL CONQUISTADOR RESORT flagship journals are now will be needed to sustain these online-only and offer open activities. Library subscriptions access options; we welcome continue to be the largest reve- the initiation of our new jour- nue source for ASPB. But pres- nal, Plant Direct; and ASPB sure from governments around is set to better interconnect the world to implement open its members with the larger access publication of publicly plant science community funded research, coupled with by way of the Plantae digital declining congressional support ecosystem. The Society stands Sally Mackenzie for basic research and state behind a new annual meeting support for public universities, in phenomics, and it is playing a growing role has influenced those revenues, compelling in helping support meetings for a number the Society to seek other ways to ensure it of plant science groups. ASPB has recently remains fiscally sound. Consequently, ASPB expanded its outreach activities to represent is creating a mix of revenue sources that will and inform plant scientists, sponsoring the allow it to continue its many good works. -
Plant Biology 2015
May/June 2015 • Volume 42, Number 3 p. 5 p. 26 p. 29 ASPB 2015 Award SURF 2015 Obituary Winners A new wave of Albert W. Frenkel ASPB honors plant undergraduate 1919–2015 scientists for their research coming exellence in research, this summer education, outreach, and service THE NEWSLETTER OF THE AMERICAN SOCIETY OF PLANT BIOLOGISTS President’s Letter Plant Biology Time to PhD—Time to Publish 2015 JULIAN SCHROEDER University of California, San Diego See You in Minneapolis! n this letter I would like necessary to generate a PhD to address two interlinked thesis in the life sciences. This Iproblems and offer possible very long training period and solutions to both of them: (1) the limited number of academic July 26–30! the relatively long duration of job openings can discour- PhD graduate studies in many age talented young scientists countries and (2) the increasing from pursuing PhDs and their time it can take from research careers in the plant sciences. advance to publication. In the On the upside, recent analy- latter case, I will also address ses have highlighted the need innovations that are being for trained PhD plant scientists Julian Schroeder implemented toward reducing (http://bit.ly/ZsHLrj), and the time to publication by The Plant Cell’s a report from the STEM Connector Food new editor-in-chief, Sabeeha Merchant, and Ag Council (http://bit.ly/1EMiGEo) and her editorial team. Additionally, recent projects substantial job growth in plant- and procedures in Plant Physiology are geared to agriculture-related industries, with a combi- shortening time to publication as well. -
1 Molecular and Environmental Plant Sciences Texas A&M University
MEPS academic program review 2014 Molecular and Environmental Plant Sciences Texas A&M University External Review Molecular and Environmental Plant Sciences Graduate Program Self Study March 2014 Molecular and Environmental Plant Sciences 1 MEPS academic program review 2014 Molecular and Environmental Plant Sciences Texas A&M University Molecular and Environmental Plant Sciences Graduate Program Self Study March 2014 College of Agriculture and Life Sciences College of Geosciences College of Science Molecular and Environmental Plant Sciences 2 MEPS academic program review 2014 TABLE OF CONTENTS EXECUTIVE SUMMARY 5 1. INTRODUCTION 8 1.1 Welcome 1.2 Charge to the Review Team 2. TEXAS A&M UNIVERSITY SYSTEM 10 2.1 Texas A&M University 2.2 The College of Agriculture and Life Sciences (COALS) 2.3 Aggie Traditions 2.4 Graduate Interdisciplinary Programs 3. MEPS PROGRAM STRUCTURE 18 3.1 Program History 3.2 Program Description 3.3 University Administration of MEPS Program 3.4 Administrative Structure of the MEPS Program 3.4.1 Executive Committee and Chair 3.4.2 Program Coordinator 3.4.3 Admissions Committee 3.4.4 Symposium Committee 3.4.5 Nomination and Awards Committee 3.5 Budget Allocations and Program Expenditures 3.5.1 Funding for current graduate students 4. THE MEPS GRADUATE PROGRAM 31 4.1 Admission Requirements and Procedures 4.2 The Curriculum 4.2.1 Master of Molecular and Environmental Plant Sciences 4.2.2 Doctoral Degree 4.2.3 Degree Plan 4.2.4 Advisory Committee 4.2.5 Designated MEPS Courses 4.2.6 MEPS funded Assistantships 4.2.7 MEPS Graduate Student Engagement 5. -
Program and Abstracts Book
16th International Conference on the Cell and Molecular Biology of Chlamydomonas June 8-13, 2014 Asilomar Conference Center, Pacific Grove, CA, USA Program and Abstracts 16th International Conference on the Cell and Molecular Biology of Chlamydomonas June 8-13, 2014 Asilomar Conference Grounds Pacific Grove, California Program and Abstracts Organizers: Kris Niyogi, University of California, Berkeley Winfield Sale, Emory University Marilyn Kobayashi, University of California, Berkeley Advisory Committee: José Luis Crespo, CSIC - Universidad de Sevilla Susan Dutcher, Washington University School of Medicine Arthur Grossman, Carnegie Institution for Science Sabeeha Merchant, University of California, Los Angeles Jun Minagawa, National Institute for Basic Biology David Mitchell, SUNY Upstate Medical University Rachael Morgan-Kiss, Miami University Michael Schroda, University of Kaiserslautern Carolyn Silflow, University of Minnesota James Umen, Donald Danforth Plant Science Center Chia-Lin Wei, DOE Joint Genome Institute William Zerges, Concordia University 1 2 TABLE OF CONTENTS General Information ............................................................................................................................ 4 Exhibitors ............................................................................................................................................. 4 Schedule of Events ............................................................................................................................... 5 Plenary Session Listings -
Ournal of Botany 93(7): 942-952
American Journal of Botany 93(7): 942-952. 2006. Jub ONE HUNDRED YEARS OF AMERICAN BOTANY: A SHORT HISTORY anc Del OF THE BOTANICAL SOCIETY OF AMERICA 1 the for VASSIUK] B ETTY S MOCOVITIS Ew fiel ThE Department of Zoology and Department of History, University of FIOIi da, Gainesville, Florida 326 1I USA wa: ThE This paper offers highlights from the 100 (plus) years of the Botanical Society of America (BSA) and draws extensively on the rest archives of the BSA, In addit ion to examining the foundin g of the society and the attempt to " professionalize" botany in late 19th century America, the paper also explores the comp lex relations between the BSA and a number of related societies in the United apr States, the Society's struggle to create a coherent identity for itself, the place of botany as a who le in the context of the burgeoni ng rig! biological sciences in the 20lh centu ry, and the changing role of the BSA in an international context. The paper assesses both the em achievements and the challenges facing the BSA. It closes by offering some historical reflections on the status of "botany" as bot a science and the historical significance of terms like " plant biology" and "plant science." eXI Ge Key words: American botany; Botanical Society of America; history of botany; scientific society. for the elit ".. " the number of real botanists is increasing in this country by year." itself to shifting conditions, some of which led to the collapse - F. C. Newcombe 10 Erwin Frink Smith, 18 August /895 of other less adaptab le societies, It has, in short, demonstrated be! the kind of evolution ary history well known to students of plant ,,, Botanical' is good! It fits a downsized world because it has primary An producers in its portfolio." evo lutionary biology. -
1 Running Title: Manganese Deficiency in Chlamydomonas Corresponding Author: Sabeeha Merchant UCLA Department of Chemistry
Plant Physiology Preview. Published on December 8, 2006, as DOI:10.1104/pp.106.088609 Running title: Manganese Deficiency in Chlamydomonas Corresponding Author: Sabeeha Merchant UCLA Department of Chemistry and Biochemistry Box 951569 (post) 607 Charles E. Young Drive East (courier) Los Angeles, CA 90095-1569 Phone: +1 (310) 825-8300 Fax: +1 (310) 206-1035 Email: [email protected] Research Category: Environmental Stress and Adaptation 1 Copyright 2006 by the American Society of Plant Biologists Manganese deficiency in Chlamydomonas results in loss of PSII and MnSOD function, sensitivity to peroxides, and secondary phosphorus- and iron-deficiency Michael D. Allen1, Janette Kropat1, Stephen Tottey1, 2, José A. Del Campo1, 3, and Sabeeha S. Merchant1, * 2 This work was supported by the Department of Energy (DE-FG02-04ER15529) and the National Institutes of Health (GM42143). MDA was supported in part by Institutional and Individual Kirschstein Fellowships (GM07185 and GM077066). JdC was supported in part by a postdoctoral fellowship from the Spanish Ministry for Education. ST was supported in part by the UC Toxic Substances Research and Teaching Program. 1 Department of Chemistry and Biochemistry, UCLA, Box 951569, Los Angeles, CA 90095- 1569 2 Present address: Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom 3 Present address: Instituto de Bioquímica Vegetal y Fotosíntesis (Univ. de Sevilla-CSIC). Centro de Investigaciones Científicas Isla de la Cartuja. Avda. Americo Vespucio s/n 41092 Sevilla, Spain * corresponding author, +1 310 825-8300 (tel), +1 310 206-1035 (fax), [email protected] Note to Copy Editor: Mn, Mn2+ and manganese are used with specific meaning throughout the manuscript.