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Safinya Laboratories UCSB Lab-specific Chemical Hygiene Plan Lab-Specific Chemical Hygiene Plan (CHP) General Information & Standard Operating Procedures (SOPs) for the Safinya Laboratories (MRL rooms 1012, 1012B, 1016, 1024, 1032) Chemical Hygiene Officer (CHO): David Vandenberg ([email protected]) KE, Rev. 2/22/18 UCSB Lab-specific Chemical Hygiene Plan This page intentionally left blank 2 KE, Rev. 2/22/18 UCSB Lab-specific Chemical Hygiene Plan Table of Contents Preface ________________________________________________________________9 Introduction ___________________________________________________________10 Twelve Commandments for Lab Safety ____________________________________11 UCSB Laboratory Worker Responsibilities _________________________________12 General Laboratory Information _________________________________________13 Laboratory Supervisor (PI) ___________________________________________________________ 13 Laboratory Locations (Building /Rooms) ________________________________________________ 13 Laboratory Safety Coordinators (Safety Czars) ____________________________________________ 13 Department Information (MRL) __________________________________________14 Department Safety Representative / Hazard Communication Coordinator _______________________ 14 Location of the Department Safety Bulletin Board _________________________________________ 14 Location of MRL Building Emergency Assembly Point (EAP) _______________________________ 14 Emergency Information _________________________________________________15 Emergency procedures ___________________________________________________15 Evacuation procedures ___________________________________________________15 First-aid kit ____________________________________________________________15 Spill cleanup materials ___________________________________________________15 Laboratory monitors and alarms ____________________________________________16 Fires __________________________________________________________________16 Fire alarm and Evacuation Guidelines ___________________________________________________ 16 Reporting a fire ____________________________________________________________________ 16 Fire Extinguishers __________________________________________________________________ 16 In the Event of an Injury __________________________________________________17 Serious Injuries ____________________________________________________________________ 17 Other Injuries ______________________________________________________________________ 17 Health & Safety References ______________________________________________18 NOTE: Safety Data Sheets (SDS) (formerly known as Material Safety Data Sheets/MSDS): ____ 18 Written Safety Resources & References ______________________________________18 Electronic Safety Resources & References ____________________________________19 Important Phone Numbers and Contact List ________________________________22 Emergency phone numbers ___________________________________________________________ 22 Other important phone numbers ________________________________________________________ 22 Contact information for Lab members and Amanda Strom ___________________________________ 25 Safinya Lab Chores ____________________________________________________26 Earthquake Safety _____________________________________________________26 Lab Safety ____________________________________________________________26 3 KE, Rev. 2/22/18 UCSB Lab-specific Chemical Hygiene Plan Introduction ____________________________________________________________26 Safety Training Requirements______________________________________________27 Fundamental Lab Safety Rules and Precautions ________________________________28 General ___________________________________________________________________________ 28 Lab Equipment _____________________________________________________________________ 29 Electrical Safety ____________________________________________________________________ 29 Gas cylinder handling _______________________________________________________________ 29 Centrifuge Safety ___________________________________________________________________ 30 Pipetting Safety/Ergonomics __________________________________________________________ 30 Chemical Safety ________________________________________________________30 A Humorous View of Chemical Safety OR What to Look Out For _____________________________ 31 Transporting Chemicals ______________________________________________________________ 31 Fume Hood Usage __________________________________________________________________ 31 Identifying Chemical Hazards ______________________________________________32 Chemical safety labels _______________________________________________________________ 32 Communicating Safety and other Lab Issues __________________________________34 Personal Protective Equipment (PPE) _______________________________________34 Safety Glasses and Other Eye Protection _________________________________________________ 34 Clothing requirements _______________________________________________________________ 35 Closed-Toe Footwear ________________________________________________________________ 35 Lab coats _________________________________________________________________________ 35 Communal PPE ____________________________________________________________________ 36 Gloves ___________________________________________________________________________ 37 Glove Selection Chart _______________________________________________________________ 39 The Laboratory Hazard Assessment Tool (“LHAT”) ____________________________41 Main MRL Labs ____________________________________________________________________ 42 Cell Lab __________________________________________________________________________ 43 LOM area _________________________________________________________________________ 43 Fridges and Freezers _____________________________________________________44 Some Best Lab Practices __________________________________________________44 Using Eppendorf-type pipettes: ________________________________________________________ 44 Labeling of Samples, Solutions etc. _________________________________________45 Lab Safety Equipment ____________________________________________________46 Emergency Showers and Eyewash Stations _______________________________________________ 46 Spills and Exposure to Hazardous Chemicals _________________________________46 Emergency procedure _______________________________________________________________ 46 First Aid After Exposure to Hazardous Chemicals _________________________________________ 46 Spill Cleanup ______________________________________________________________________ 47 Disposal of Hazardous Waste ______________________________________________47 Chemical Waste Disposal ____________________________________________________________ 47 Proper Hazardous Waste Segregation ___________________________________________________ 47 Collecting and Storing Hazardous Waste ________________________________________________ 48 Labeling Hazardous Waste ___________________________________________________________ 48 Proper Waste Disposal / EH&S Pickup __________________________________________________ 49 4 KE, Rev. 2/22/18 UCSB Lab-specific Chemical Hygiene Plan Sharps disposal _________________________________________________________49 Glass Disposal _____________________________________________________________________ 49 Disposal of Razor Blades, Needles, etc. _________________________________________________ 50 Completing Work in the Lab – A Checklist _________________________________50 Get Started ________________________________________________________________________ 50 Get Clear _________________________________________________________________________ 50 Stay Connected ____________________________________________________________________ 51 Standard Operating Procedures (SOPs) ____________________________________52 Background: Standard Operating Procedures __________________________________52 SOP: Use of Acrylonitrile ________________________________________________53 Date of last revision to SOP: Aug. 2014 – Kai Ewert _______________________________________ 53 PI approval of SOP: _________________________________________________________________ 53 Scope of SOP ______________________________________________________________________ 53 Compound information ______________________________________________________________ 53 Approval Required __________________________________________________________________ 53 General precautions _________________________________________________________________ 53 Personal Protective Equipment ________________________________________________________ 54 Engineering/Ventilation Controls ______________________________________________________ 54 Handling, Storage, Cleanup, First Aid, and Disposal Requirements ____________________________ 54 Additional Information ______________________________________________________________ 55 LCSS for ACRYLONITRILE ______________________________________________56 Documentation of Training – SOP Use of Acrylonitrile _________________________58 SOP: Use of Benzene ____________________________________________________59 Date of last revision to SOP: Aug. 2014 – Kai Ewert _______________________________________ 59 PI approval of SOP: _________________________________________________________________ 59 Scope of SOP ______________________________________________________________________ 59 Compound information ______________________________________________________________ 59
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