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June 09 Mon Posters POSTERS WEDNESDAY, JUNE 12 POSTER SESSION HALLS 11.1, 11.2 10.00 - 12.00 WEDNESDAY Basic Science Inorganic ions W001-W034 Protein sorting / Epithelial polarity W035-W042 Extracellular mediators (cytokines, prostanoids, nucleotides, radicals, etc.) W043-W086 Extracellular matrix, fibrosis W087-W143 Renal haemodynamics, vascular physiology, vascular pathology, experimental hypertension W144-W188 Miscellaneous - Tubular cell function W189-W203 Miscellaneous - Arrays and proteonomics W204-W209 General Nephrology Primary glomerular diseases: clinical W210-W235 Vasculitides, systemic diseases: clinical W236-W252 Hypertension: clinical W253-W264 Hereditary renal diseases W265-W296 Clinical nephrology: miscellaneous W297-W331 Acute renal failure, toxic nephropathy W332-W389 Chronic renal failure: miscellaneous W390-W412 Calcium, magnesium, phosphate and phosphate binders W413-W439 Anaemia: miscellaneous W440-W457 Dialysis Cardiov. risk in ESRD, homocysteine W458-W483 Cardiov. risk in ESRD, cardiac hypertrophy and atherosclerosis W484-W515 Cardiovascular morbidity and mortality; lipids, inflammation and other predictors W516-W566 Vascular access W567-W622 ESRD treatment comparisons W623-W636 Haemodialysis: infections, cancer W637-W648 Haemofiltration, Haemodiafiltration and other modalities W649-W670 PD: Cardiov. morbidity and mortality W671-W679 PD: inflammation, nutrition W680-W697 PD: miscellaneous W698-W720 Transplantation Immunosuppression - clinical trials W721-W750 Immunosuppression – other W751-W763 Outcomes, cardiov. morbidity and mortality W764-W789 Miscellaneous W790-W805 Paediatric Nephrology Physiology W806-W814 Pathology W815-W817 Pharmacology W818-W822 Paediatric clinical nephrology, acute and chronic renal failure, bone and mineral metabolism, anaemia Miscellaneous W823-W846 Acute renal failure W847-W850 Paediatric dialysis Peritoneal dialysis W851-W866 Paediatric transplantation Infections W867-W873 355 WEDNESDAY, JUNE 12 POSTERS BASIC SCIENCE INORGANIC IONS W001 THE cAMP-INDUCED INCREASE IN Na,K-ATPase CELL SURFACE EXPRESSION IS INDEPENDENT OF THE PHOSPHORYLATION OF ITS ALPHA-SUBUNIT ON Ser-943 IN CULTURED COLLECTING DUCT PRINCIPAL CELLS David Mordasini,1 Marcelle Bens,2 Pierre-Yves Martin,1 Alain Vandewalle,2 Eric Feraille.1 1Division of Nephrology, University of Geneva, Geneva, Switzerland; 2INSERM U478, Faculté de Médecine Xavier Bichat, Paris, France W002 EXPLORATION OF THE CATION PATHWAY THROUGH THE Na,K-ATPASE BY CYSTEINE SCANNING MUTAGENESIS: CONTRIBUTION OF THE 4TH TRANSMEMBRANE SEGMENT OF THE a SUBUNIT Jean-Daniel Horisberger,1 Solange Kharoubi-Hess,1 Saida Guennoun.2 1Institute of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland; 2Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, Newark, NJ, United States W003 THE SKIN IS AN OSMOTICALLY- INACTIVE NA+ RESERVOIR THAT IS DIMINISHED IN DAHL RATS Jens Titze,1 Peter Dietsch,2 Karl-Heinz Schwind,3 Karl A. Kirsch,4 Karl F. Hilgers.1 1Nephrology, Friedrich-Alexander-Univ. Erlangen-Nürnberg, Erlangen, Germany; 2Biochemistry, Free Univ. Berlin, Germany; 3Chemistry and Physics, Federal Center for Meat Research, Kulmbach, Germany; 4Physiology, Free Univ.Berlin, Berlin, Germany W004 OVARIECTOMY (OVX) LEADS TO AQUIRED INCAPACITY OF OSMOTICALLY-INACTIVE NA+ STORAGE IN SPRAGUE DAWLEY RATS Jens Titze,1 Mehdi Shakibaei,2 Peter Dietsch,3 Gundula Schulze- Tanzil,2 Karl A. Kirsch,4 Karl-Heinz Schwind,5 Karl F. Hilgers.1 1Nephrology, Friedrich Alexander University Erlangen-Nürnberg, Erlangen, Germany; 2Anatomy, Free University Berlin, Berlin, Germany; 3Biochemistry, Free University Berlin, Berlin, Germany; 4Physiology, Free University Berlin, Berlin, Germany; 5Chemistry & Physics, Federal Center for Meat Research, Kulmbach, Germany W005 A SINGLE NUCLEOTIDE POLYMORPHISM THAT ALTERS THE ACTIVITY OF THE RENAL Na+:Cl- COTRANSPORTER Erika Moreno,1,2 Patricia Meade,1,2 Norma Vazquez,1,2 Gerardo Gamba.1,2 1Nephrology and Mineral Metabolism, Instituto Nacional de Ciencias Medicas y Nutricion SZ, Tlalpan, Mexico City, Mexico; 2Molecular Physiology Unit, Instituto de Investigaciones Biomedicas, UNAM, Tlalpan, Mexico City, Mexico W006 NKCC1, THE SECRETORY Na+-K+-Cl- COTRANSPORTER, MEDIATES NH4+ AND Cl- UPTAKE DURING AMMONIUM- INDUCED FLUID SECRETION IN THE RAT INNER MEDULLARY COLLECTING DUCT Darren Wallace, Gail Reif, Anne-Marie Hedge, Jared Grantham. Kidney Institute, Univ. of Kansas Medical Center, Kansas City, KS, USA W007 STIMULATION OF NEDD4-2 PHOSPHORYLATION AND TRANSEPITHELIAL NA-TRANSPORT BY ALDOSTERONE IN MPKCCDcl4 CELLS Sandra Y. Flores,1 Johannes Loffing,2 Olivier Staub.1 1Institute of Pharmacology & Toxicology, Univ.of Lausanne, Lausanne, Switzerland; 2Institute of Anatomy, Univ. of Zurich, Zurich, Switzerland 356 POSTERS WEDNESDAY, JUNE 12 W008 INACTIVATION OF ALPHA ENAC SUBUNIT FUNCTION IN THE COLLECTING DUCT Isabelle Rubera,1 Jan Loffing,2 Larry Palmer,3 Tom Carrol,4 Andy McMahon,4 Bernard Rossier,1 Edith Hummler.1 WEDNESDAY 1Fac Med, Inst Pharmacol & Toxicologie, Lausanne, Switzerland; 2Univ of Zurich, Inst.of Anatomy, Zurich, Switzerland; 3Dept of Physiology and Biophysics, New York, USA; 4Dept of Molecular and Cellular Biology, Cambridge, USA W009 MUTATIONS IN THE OUTER PORE OF THE EPITHELIAL NA CHANNEL DISRUPT AMILORIDE BLOCK BY INCREASING THE OFF-RATE Stephan Kellenberger, Ivan Gautschi, Laurent Schild. Inst. de Pharmacologie et de Toxicologie, Univ. de Lausanne, Lausanne, VD, Switzerland W010 TUBULAR SECRETION OF PHOSPHATE IN THE NORMAL RAT: EFFECT OF ANGIOTENSIN CONVERTING ENZYME INHIBITION Isabelle M. Renaud,1,2 Angele Chainey,2 Jacques Chevalier,1 Gerard E. Plante.2 1INSERM U430, Broussais Hospital, Paris, France; 2Pharmacology Institute, Sherbrooke University, Sherbrooke, QC, Canada W011 APICAL TRANSLOCATION OF ROMK AND ENaC IN MOUSE DISTAL NEPHRON IN RESPONSE TO HIGH K+ DIET Dominique Loffing-Cueni,1 Martin Schlaepfer,1 May Bloch-Faure,3 Edith Hummler,2 Bernard Rossier,2 Brigitte Kaissling,1 Pierre Meneton,3 Johannes Loffing.1 1Institute of Anatomy, University of Zurich, Zurich, Switzerland; 2Institute of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland; 3INSERM U367, Paris, France W012 DYSFUNCTION OF THE EPITHELIAL SODIUM CHANNEL (ENaC) EXPRESSED IN THE KIDNEY OF LIDDLE MICE Sylvain Pradervand,1 Alain Vandewalle,2 Marcelle Bens,2 Ivan Gautschi,1 Johannes Loffing,3 Edith Hummler,1 Laurent Schild,1 Bernard Rossier.1 1Inst. de Pharmacologie et de Toxicologie, Univ. de Lausanne, Lausanne, Vaud, Switzerland; 2U478, Fac. de Medecine Xavier Bichat, Paris, France; 3Anatomisches Inst., Univ. Zurich-Irchel, Zurich, Switzerland W013 REGULATION OF EPITHELIAL NA+ CHANNELS IN A MOUSE MODEL OF LIDDLE’ S SYNDROM Anke Dahlmann,1 Edith Hummler,2 Sylvain Pradervand,2 Bernard Rossier,2 Gustavo Frindt,1 Lawrence G. Palmer.1 1Dept of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY, United States; 2Institut de Pharmacologie et de Toxicologie, Université de Lausanne, Lausanne, Switzerland W014 THE EFFECT OF NACL ON LINGUAL SURFACE POTENTIALS AND THE ROLE OF AMILORIDE George Feldman,1 Gerard Heck,2 Vijay Lyall,2 Cecily Santos,1 Rebeca Clary,1 John DeSimone,2 Andras Mogyorosi.1 1Medicine, VCU School of Medicine and McGuire VAMC, Richmond, VA, USA; 2Physiology, VCU School of Medicine, Richmond, VA, USA W015 MECHANISMS UNDERLYING THE REGULATION OF THE RENAL OUTER MEDULLARY K+ CHANNEL ROMK1 BY THE SERUM AND GLUCOCORTICOID INDUCIBLE KINASE SGK1 AND THE NA+/H+ EXCHANGER REGULATING FACTOR NHERF2 Monica Palmada,1 Hamdy M. Embark,1 Chris Yun,2 Edward J. Weinman,3 David Pearce,4 Christoph Boehmer,1 Florian Lang.1 1Dept Physiology, Eberhard-Karls-Univ, Tuebingen, Germany; 2Dept Medicine, Emory Univ, Atlanta, GA, USA; 3Dept Medicine, Univ Maryland, Baltimore, MD, USA; 4Dept Medicine, Univ California, San Francisco, CA, USA 357 WEDNESDAY, JUNE 12 POSTERS W016 CONTRIBUTION OF THE INTRACELLULAR N-TERMINUS OF aENaC IN ITS FUNCTION AND STIMULATION BY ALDOSTERONE IN A CORTICAL COLLECTING DUCT CELL LINE Muriel Auberson, Laurent Schild. Institut de Pharmacologie et Toxicologie, Université de Lausanne, Lausanne, Vaud, Switzerland W017 RENAL PHENOTYPE OF THE TWIK1 POTASSIUM CHANNEL KNOCKOUT MOUSE Richard Warth,1 Isabelle Arrighi,2 Raymond Mengual,2 Elisa Romeo,1 Francois Verrey,1 Jacques Barhanin.2 1Institute of Physiology, University of Zurich, Zurich, Switzerland; 2IPMC, CNRS, Sophia-Antipolis, France W018 RENAL POTASSIUM EXCRETION IS IMPAIRED IN SGK1 DEFICIENT MICE Danyang Huang,1 Peer Wulff,2 Dietmar Kuhl,3 Florian Lang,4 Volker Vallon.1 1Institute of Pharmacology and Toxicology, University of Tuebingen, Tuebingen, Baden-Wuerttemberg, Germany; 2Clinic of Neurology, University of Heidelberg, Heidelberg, Baden-Wuerttemberg, Germany; 3Institute of Molecular Neurobiology, Free University of Berlin, Berlin, Germany; 4Institute of Physiology, University of Tuebingen, Tuebingen, Baden-Wuerttemberg, Germany W019 ABSENCE OF INTERMEDIATE-CONDUCTANCE K CHANNEL (IK) IN THE APICAL MEMBRANE OF THICK ASCENDING LIMB (TAL) IN ROMK KNOCKOUT MICE Ming Lu, Qingshang Yan, Tong Wang, Steven Hebert, Gerhard Giebisch. Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, United States W020 CHARACTERIZATION OF THE FUNCTION OF TWO NATURALLY OCCURRING MUTATIONS IN THE HUMAN SODIUM- PHOSPHATE COTRANSPORTER TYPE IIa Leila Virkki, Ian Forster, Jürg Biber, Heini Murer. Institute of Physiology, University of Zürich, Zürich, Switzerland W021 ONTOGENY, PROCESSING
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