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1 Supplementary Tables 1 2 Fine-Scale Adaptations To 1 SUPPLEMENTARY TABLES 2 3 FINE-SCALE ADAPTATIONS TO ENVIRONMENTAL VARIATION AND GROWTH 4 STRATEGIES DRIVE PHYLLOSPHERE METHYLOBACTERIUM DIVERSITY. 5 6 Jean-Baptiste Leducq1,2,3, Émilie Seyer-Lamontagne1, Domitille Condrain-Morel2, Geneviève 7 Bourret2, David Sneddon3, James A. Foster3, Christopher J. Marx3, Jack M. Sullivan3, B. Jesse 8 Shapiro1,4 & Steven W. Kembel2 9 10 1 - Université de Montréal 11 2 - Université du Québec à Montréal 12 3 – University of Idaho 13 4 – McGill University 14 1 15 Table S1 - List of reference Methylobacteriaceae genomes from which the complete rpoB 16 and sucA nucleotide sequences were available. Following information is shown: strain name, 17 given genus name (Methylorubrum considered as Methylobacterium in this study), given species 18 name (when available), biome where the strain was isolated from (when available; only indicated 19 for Methylobacterium), groups (B,C) or clade (A1-A9) assignation, and references or source 20 (NCBI bioproject) when unpublished. Complete and draft Methylobacteriaceae genomes publicly 21 available in September 2020, including 153 Methylobacteria, 30 Microvirga and 2 Enterovirga, 22 using blast of the rpoB complete sequence from the M. extorquens strain TK001 against NCBI 23 databases refseq_genomes and refseq_rna (1) available for Methylobacteriaceae 24 (Uncultured/environmental samples excluded) STRAIN Genus Species ENVIRONMENT Group/ Reference/source Clade BTF04 Methylobacterium sp. Water A1 (2) Gh-105 Methylobacterium gossipiicola Phyllosphere A1 (3) GV094 Methylobacterium sp. Rhizhosphere A1 https://www.ncbi.nlm.nih.gov/bioproject /443353 GV104 Methylobacterium sp. Rhizhosphere A1 https://www.ncbi.nlm.nih.gov/bioproject /443354 Leaf100 Methylobacterium sp. Phyllosphere A1 (4) Leaf102 Methylobacterium sp. Phyllosphere A1 (4) Leaf104 Methylobacterium sp. Phyllosphere A1 (4) Leaf111 Methylobacterium sp. Phyllosphere A1 (4) Leaf112 Methylobacterium sp. Phyllosphere A1 (4) Leaf113 Methylobacterium sp. Phyllosphere A1 (4) Leaf117 Methylobacterium sp. Phyllosphere A1 (4) Leaf125 Methylobacterium sp. Phyllosphere A1 (4) Leaf465 Methylobacterium sp. Phyllosphere A1 (4) Leaf469 Methylobacterium sp. Phyllosphere A1 (4) Leaf87 Methylobacterium sp. Phyllosphere A1 (4) Leaf88 Methylobacterium sp. Phyllosphere A1 (4) Leaf89 Methylobacterium sp. Phyllosphere A1 (4) Leaf94 Methylobacterium sp. Phyllosphere A1 (4) Leaf99 Methylobacterium sp. Phyllosphere A1 (4) V23 Methylobacterium sp. Water A1 (5) WL69 Methylobacterium sp. Phyllosphere A1 (6) 10 Methylobacterium sp. Water A2 https://www.ncbi.nlm.nih.gov/bioproject /195835 77 Methylobacterium sp. Water A2 https://www.ncbi.nlm.nih.gov/bioproject /165569 88A Methylobacterium sp. Water A2 https://www.ncbi.nlm.nih.gov/bioproject /165571 Leaf106 Methylobacterium sp. Phyllosphere A2 (4) Leaf85 Methylobacterium sp. Phyllosphere A2 (4) Leaf86 Methylobacterium sp. Phyllosphere A2 (4) Leaf91 Methylobacterium sp. Phyllosphere A2 (4) Leaf93 Methylobacterium sp. Phyllosphere A2 (4) WL19 Methylobacterium sp. Phyllosphere A2 (6) Leaf108 Methylobacterium sp. Phyllosphere A3 (4) Leaf399 Methylobacterium sp. Phyllosphere A3 (4) Leaf466 Methylobacterium sp. Phyllosphere A3 (4) DSM24105 Methylobacterium brachythecii Phyllosphere A4 (7) 2 NBRC107714 Methylobacterium haplocladii Phyllosphere A4 (7) NBRC107716 Methylobacterium gnaphalii Phyllosphere A4 (8) WL9 Methylobacterium sp. Phyllosphere A4 (6) 17J42-1 Methylobacterium segetis Soil A5 (9) 17SD2-17 Methylobacterium durans Anthropo/Soil A5 (10) NBRC107715 Methylobacterium oxalidis Phyllosphere A5 (11) YIM132548 Methylobacterium planium Lichen A5 (12) YIM48816 Methylobacterium soli Soil A5 (13) WL103 Methylobacterium sp. Phyllosphere A6 (6) WL116 Methylobacterium sp. Phyllosphere A6 (6) WL119 Methylobacterium sp. Phyllosphere A6 (6) WL12 Methylobacterium sp. Phyllosphere A6 (6) WL120 Methylobacterium sp. Phyllosphere A6 (6) WL30 Methylobacterium sp. Phyllosphere A6 (6) WL6 Methylobacterium sp. Phyllosphere A6 (6) WL8 Methylobacterium sp. Phyllosphere A6 (6) WL93 Methylobacterium sp. Phyllosphere A6 (6) 17Sr1-43 Methylobacterium sp. Anthropo/Soil A7 (14) CCH5-D2 Methylobacterium sp. Anthropo/Water A7 (15) SB0023/3 Methylobacterium symbioticum Fungus A8 (16) SW08-7 Methylobacterium dankookense Anthropo/Water A8 (17) 111MFTsu3.1M4 Methylobacterium brachiatum Plant A9 (18) 13MFTsu3.1M2 Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /351394 190mf Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /350406 275MFSha3.1 Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /347448 285MFTsu5.1 Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /187943 2A Methylobacterium sp. Rhizhosphere A9 (19) ARG-1 Methylobacterium sp. Fungus A9 (20) B1 Methylobacterium sp. Phyllosphere A9 (21) B34 Methylobacterium sp. Phyllosphere A9 (21) BK227 Methylobacterium sp. Endophyte A9 https://www.ncbi.nlm.nih.gov/bioproject /520059 BL36 Methylobacterium pseudosasicola Phyllosphere A9 (22) BL47 Methylobacterium phyllostachyos Phyllosphere A9 (22) C1 Methylobacterium sp. Anthropo/Water A9 (23) CBMB20 Methylobacterium oryzae Phyllosphere A9 (24) CBMB27 Methylobacterium phyllosphaerae Phyllosphere A9 (25) DSM5686 Methylobacterium fujisawaense Unknown A9 (26, 27) DSM760 Methylobacterium organophilum Water A9 (27, 28) ES_PA-B5 Methylobacterium radiotolerans Rhizhosphere A9 https://www.ncbi.nlm.nih.gov/bioproject /PRJNA460942 GXF4 Methylobacterium sp. Endophyte A9 (29) GXS13 Methylobacterium sp. Endophyte A9 (30) JCM2831 Methylobacterium radiotolerans Seed A9 (31) = NBRC15690 Leaf361 Methylobacterium sp. Phyllosphere A9 (4) MAMP4754 Methylobacterium radiotolerans Endophyte A9 (32) ME94 Methylobacterium radiotolerans Endophyte A9 (33) P1-11 Methylobacterium sp. Rhizhosphere A9 (34) RE1.2 Methylobacterium radiotolerans Seed A9 https://www.ncbi.nlm.nih.gov/bioproject /278124 SR1.6/6 Methylobacterium mesophilicum Phyllosphere A9 (35) TX0642 Methylobacterium brachiatum Anthropo A9 (36) UNC300MFChir4. Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject 1 /351052 3 UNC378MF Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /248486 UNCCL110 Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /234870 UNCCL125 Methylobacterium sp. Plant A9 https://www.ncbi.nlm.nih.gov/bioproject /PRJNA248500 WL1 Methylobacterium sp. Phyllosphere A9 (6) WL18 Methylobacterium sp. Phyllosphere A9 (6) WL2 Methylobacterium sp. Phyllosphere A9 (6) WL64 Methylobacterium sp. Phyllosphere A9 (6) WL7 Methylobacterium sp. Phyllosphere A9 (6) XJLW Methylobacterium sp. Anthropo/Water A9 (37) YL-MPn5-2016 Methylobacterium sp. Water A9 https://www.ncbi.nlm.nih.gov/bioproject /618225 YL-MPn6-2016 Methylobacterium sp. Water A9 https://www.ncbi.nlm.nih.gov/bioproject /618226 yr668 Methylobacterium sp. Rhizhosphere A9 https://www.ncbi.nlm.nih.gov/bioproject /303324 AM1 Methylobacterium extorquens Anthropo B (38) AMS5 Methylobacterium sp. Phyllosphere B (39) B4 Methylobacterium sp. Anthropo/Water B https://www.ncbi.nlm.nih.gov/bioproject /368293 BJ001 Methylobacterium populi Endophyte B (31) CD11_7 Methylobacterium populi Anthropo/Microbio B (40) me CGMCC1.6474 Methylobacterium salsuginis Ocean B (41) CLZ Methylobacterium sp. Soil B (42) CM4 Methylobacterium extorquens Anthropo/Soil B (31) CP3 Methylobacterium extorquens Seed B (43) DB1607 Methylobacterium sp. Anthropo B https://www.ncbi.nlm.nih.gov/bioproject /495120 DM1 Methylobacterium sp. Anthropo B (44) DM4 Methylobacterium extorquens Anthropo/Water B (38) DSM11490 Methylobacterium thiocyanatum Rhizhosphere B (27, 45) DSM13060 Methylobacterium extorquens Phyllosphere B (46) DSM2163 Methylobacterium rhodinum Soil B (27, 47) DSM5687 Methylobacterium rhodesianum Anthropo B (26, 27) L1A1 Methylobacterium sp. Water B (48) Leaf123 Methylobacterium sp. Phyllosphere B (4) Leaf456 Methylobacterium sp. Phyllosphere B (4) MB200 Methylobacterium sp. Anthropo B (49) NBRC15911 Methylobacterium extorquens Anthropo B https://www.ncbi.nlm.nih.gov/bioproject /PRJDB6075 NI91 Methylobacterium sp. Soil B (42) P-1M Methylobacterium populi Anthropo/Water B (50) PA1 Methylobacterium extorquens Phyllosphere B (31) PinkelPinkel_01 Methylobacterium populi Anthropo/Soil B (51) PSBB040 Methylobacterium extorquens Water B https://www.ncbi.nlm.nih.gov/biosampl e/SAMN06210717 PSBB041 Methylobacterium zatmanii Water B https://www.ncbi.nlm.nih.gov/bioproject /PRJNA376590 Q1 Methylobacterium sp. Endophyte B https://www.ncbi.nlm.nih.gov/bioproject /529698 R2-1 Methylobacterium sp. Unknown B https://www.ncbi.nlm.nih.gov/biosampl e/SAMN12024188/ RAS18 Methylobacterium sp. Phyllosphere B (52) TK0001 Methylobacterium extorquens Soil B (53) YC-XJ1 Methylobacterium populi Unknown B (54) 174MFSha1.1 Methylobacterium sp. Plant C https://www.ncbi.nlm.nih.gov/bioproject /248487 17Sr1-1 Methylobacterium sp. Soil C https://www.ncbi.nlm.nih.gov/nuccore/1 393188502 17Sr1-28 Methylobacterium terrae Anthropo/Soil C (55) 4 17Sr1-39 Methylobacterium terricola Soil C (56) 4-46 Methylobacterium sp. Rhizhosphere C (31) 6HR-1 Methylobacterium nonmethylotrophicum Anthropo/Soil C (57) ap11 Methylobacterium sp. Rhizhosphere C https://www.ncbi.nlm.nih.gov/bioproject /303350 DB0501 Methylobacterium sp. Anthropo C https://www.ncbi.nlm.nih.gov/bioproject /606480 DSM16371 Methylobacterium aquaticum Anthropo/Water C (58) =GR16 DSM16961 Methylobacterium variabile Anthropo/Water C (59) DSM25844 Methylobacterium tarhaniae Soil C (60) JCM14648 Methylobacterium
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