Early Decrease in Serum Amphiregulin Or Vascular Endothelial Growth Factor Levels Predicts Sorafenib Efficacy in Hepatocellular Carcinoma

Total Page:16

File Type:pdf, Size:1020Kb

Early Decrease in Serum Amphiregulin Or Vascular Endothelial Growth Factor Levels Predicts Sorafenib Efficacy in Hepatocellular Carcinoma ONCOLOGY REPORTS 41: 2041-2050, 2019 Early decrease in serum amphiregulin or vascular endothelial growth factor levels predicts sorafenib efficacy in hepatocellular carcinoma CORINNE GODIN1,2*, SANDRA BODEAU2,3*, ZUZANA SAIDAK1,4, CHRISTOPHE LOUANDRE2, CATHERINE FRANÇOIS5, JEAN-CLAUDE BARBARE6, ROMAIN CORIAT7,8, ANTOINE GALMICHE1,2 and CHLOÉ SAUZAY1,2 1Equipe CHIMERE, EA 7516, Université de Picardie Jules Verne; 2Laboratoire de Biochimie; 3Laboratoire de Pharmacologie; 4Service d'Oncobiologie Moléculaire, Centre de Biologie Humaine, CHU Sud; 5EA4294, Université de Picardie Jules Verne; 6Délégation à la Recherche Clinique et à l'Innovation, CHU Sud, 80054 Amiens; 7Gastroenterology and Digestive Oncology Unit, Cochin University Hospital, APHP; 8Unité Inserm U1016, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France Received June 29, 2018; Accepted November 15, 2018 DOI: 10.3892/or.2018.6922 Abstract. Sorafenib is the standard of care for the treatment at the transcriptional and post-transcriptional levels. All HCC of advanced hepatocellular carcinoma (HCC). However, patients in our cohort had detectable concentrations of AREG identifying secreted biomarkers that predict sorafenib efficacy and VEGF both at baseline and after sorafenib treatment. The in all HCC patients remains challenging. It was recently decreased serum levels of AREG and VEGF after 15 days of reported that sorafenib interferes with protein homeostasis sorafenib treatment were significantly associated with better and inhibits global translation in tumour cells. A likely overall and progression-free survival. The results of the consequence of this inhibition would be the interruption multivariate analysis demonstrated that a decrease in AREG of autocrine loops. The aim of the present study was to was an independent prognostic indicator of overall survival investigate the effect of sorafenib on two growth factors (hazard ratio, 0.208; 95% confidence interval, 0.173‑0.673; implicated in autocrine loops and HCC tumour invasion: P=0.0003). These results suggest that sorafenib inhibits auto‑ amphiregulin (AREG) and vascular endothelial growth crine loops and that early decrease in serum AREG or VEGF factor (VEGF). ELISA, quantitative polymerase chain levels predicts sorafenib efficacy in HCC patients. reaction analysis, western blotting and a cytokine array were performed on HCC cell lines and the prognostic role of these Introduction two biomarkers in HCC patients was evaluated. Serum AREG and VEGF levels were assayed by ELISA in 55 patients with Liver cancer is the fifth most prevalent cancer and the second advanced HCC treated with sorafenib. It was observed that most frequent cause of cancer-related mortality worldwide (1). sorafenib decreased AREG, VEGF and cytokine expression Hepatocellular carcinoma (HCC) is the most frequent type of primary liver tumour. The majority of patients are diagnosed at an advanced stage, which explains the poor prognosis of this cancer. Sorafenib is the standard of care for the treat- Correspondence to: Dr Chloé Sauzay, Laboratoire de Biochimie, ment of advanced HCC. Sorafenib is a multikinase inhibitor Centre de Biologie Humaine, Avenue René Laennec, Centre de directed against the RAF kinases and several receptor tyrosine Biologie Humaine, CHU Sud, 80054 Amiens, France kinases, such as vascular endothelial growth factor receptor E-mail: [email protected] (VEGFR) (2). The efficacy of sorafenib was demonstrated * a decade ago in two comparative placebo-controlled trials Contributed equally that showed a benefit in terms of overall survival (OS) (3,4). Recently, lenvatinib demonstrated a survival advantage Abbreviations: AREG, amphiregulin; EGFR, epidermal growth factor receptor; OS, overall survival; PFS, progression-free survival, that was non-inferior to that of sorafenib in an open-label, UPR, unfolded protein response; VEGF, vascular endothelial growth multicentre, non-inferiority, randomised trial (5). However, factor sorafenib currently remains the only first-line treatment approved by the Food and Drug Administration for patients Key words: amphiregulin, vascular endothelial growth factor, with advanced HCC. sorafenib, hepatocellular carcinoma, prognostic biomarker, There is currently a lack of biomarkers able to predict the proteostasis efficacy of sorafenib in HCC patients. α-Fetoprotein (AFP) is the biomarker most widely used by clinicians for the follow-up of HCC patients treated by sorafenib. However, 2042 GODIN et al: DECREASE OF AREG OR VEGF PREDICTS SORAFENIB EFFICACY IN HCC AFP is secreted in only ~50% of HCC patients due to high were taken into consideration. This cut-off was based on the intratumoural variability (6,7). Among these patients, only study of Personeni et al (8). 37% exhibit a significant decrease in their AFP levels following sorafenib treatment (8). Recently, the latest recommendations Determination of AREG and VEGF concentrations. AREG of the European Association for the Study of the Liver or VEGF concentrations in cell supernatant or serum have underlined the suboptimal performance of AFP as samples were determined using a sandwich-based ELISA kit a serological test in the surveillance of HCC patients (1). (R&D Systems, Inc., Minneapolis, MN, USA) according to the Identifying a biomarker secreted in all patients with HCC that manufacturer's instructions. Only >5% decreases in the serum is directly regulated by sorafenib remains a challenge. In a level of AREG or VEGF were taken into consideration This recent study exploring the impact of sorafenib on proteostasis, cut-off was selected as it corresponded to the smallest varia- we reported that sorafenib inhibits global protein biosynthesis tion observed in our cohort. and initiation of translation (9). Proteostasis may be defined as the process regulating the production, folding, trafficking Reverse transcription‑quantitative polymerase chain reac‑ and degradation of proteins within the cell, in order to tion (RT‑qPCR) analysis. Total RNA was extracted using maintain its homeostasis. In HCC cells exposed to sorafenib, RNeasy Mini kit (ref 74104; Qiagen, Courtaboeuf, France) this inhibition of translation is expected to largely prevent the and reverse-transcribed to cDNA using High Capacity cDNA synthesis of several biologically active peptides and proteins. Reverse Transcription kit (ref 4368814; Applied Biosystems; Although this putative effect has not been formally examined, Thermo Fisher Scientific, Inc., Waltham, MA, USA) a likely consequence of protein synthesis inhibition would be according to the manufacturer's instructions. Amplification interruption of autocrine loops (10). was performed with the TaqMan Universal PCR master Mix Proliferation of HCC cells is dependent on two well-docu- (ref 4304437; Applied Biosystems; Thermo Fisher Scientific, mented autocrine loops composed of VEGF/VEGFR and Inc.) on an ABI 7900HT Sequence Detection System amphiregulin (AREG)/epidermal growth factor receptor (Applied Biosystems; Thermo Fisher Scientific, Inc.) using (EGFR) ligands and receptors. VEGFR is a direct target of primers and probe sets for AREG (ref Hs00950669_m1, cat. sorafenib and is known to be an important factor in HCC no. 433182; Applied Biosystems; Thermo Fisher Scientific, growth (11). VEGF is a potent angiogenic factor and is Inc.) and glyceraldehyde phosphate dehydrogenase (GAPDH; upregulated in several human tumours (12). This autocrine loop ref Hs99999905_m1, cat. no. 4333764; Applied Biosystems; plays a crucial role in tumour invasion and aggressiveness (12). Thermo Fisher Scientific, Inc.). The cycling conditions were Activation of EGFR during hepatocarcinogenesis is not initial denaturation at 95˚C for 10 min, followed by 45 cycles dependent on activating mutations, but rather on autocrine of denaturation at 95˚C for 15 sec and annealing at 60˚C loops involving natural ligands of EGFR, such as AREG (13). for 1 min. Data were normalized to the endogenous control AREG is upregulated in HCC and acts as a pro-oncogenic GAPDH to obtain ΔCq. The fold change in genes of interest factor (14). This autocrine loop was recently identified as a key relative to untreated samples was determined using the determinant of resistance to sorafenib (15,16). The aim of the 2-ΔΔCq method (17). present study was to evaluate the effect of sorafenib on AREG and VEGF production in two HCC cell lines. In order to study Western blotting. For each experimental condition, complete a potential link between AREG and VEGF production and the cell extracts were prepared in RIPA buffer. Following protein efficacy of sorafenib, the prognostic impact of each of these concentration determination with a BCA kit (Thermo Fisher factors was investigated in HCC patients. Scientific, Inc.), a total of 50 µg protein was precipitated with methanol and chloroform. The samples were then denatured Materials and methods in Laemmli sample buffer, loaded on 10% SDS-PAGE, and transferred to nitrocellulose membranes using standard proce- Cell culture and reagents. The human HCC cell lines used in dures. The membranes were saturated for 1 h in 5% milk in this study (Huh7 and Hep3B, passages between 6 and 8) were TTBS [Tween 0.05%, NaCl 200 mM, Tris-HCl (pH 8.0)], then obtained from Dr Wychowski (Institut de Biologie de Lille, rinsed and incubated overnight with each primary antibody at Lille, France) and were authenticated by profiling short tandem a 1:1,000 dilution (AREG, mouse, Santa Cruz Biotechnology, repeats at 16 loci (LGC Standards, Strasbourg,
Recommended publications
  • ARTICLES Fibroblast Growth Factors 1, 2, 17, and 19 Are The
    0031-3998/07/6103-0267 PEDIATRIC RESEARCH Vol. 61, No. 3, 2007 Copyright © 2007 International Pediatric Research Foundation, Inc. Printed in U.S.A. ARTICLES Fibroblast Growth Factors 1, 2, 17, and 19 Are the Predominant FGF Ligands Expressed in Human Fetal Growth Plate Cartilage PAVEL KREJCI, DEBORAH KRAKOW, PERTCHOUI B. MEKIKIAN, AND WILLIAM R. WILCOX Medical Genetics Institute [P.K., D.K., P.B.M., W.R.W.], Cedars-Sinai Medical Center, Los Angeles, California 90048; Department of Obstetrics and Gynecology [D.K.] and Department of Pediatrics [W.R.W.], UCLA School of Medicine, Los Angeles, California 90095 ABSTRACT: Fibroblast growth factors (FGF) regulate bone growth, (G380R) or TD (K650E) mutations (4–6). When expressed at but their expression in human cartilage is unclear. Here, we deter- physiologic levels, FGFR3-G380R required, like its wild-type mined the expression of entire FGF family in human fetal growth counterpart, ligand for activation (7). Similarly, in vitro cul- plate cartilage. Using reverse transcriptase PCR, the transcripts for tivated human TD chondrocytes as well as chondrocytes FGF1, 2, 5, 8–14, 16–19, and 21 were found. However, only FGF1, isolated from Fgfr3-K644M mice had an identical time course 2, 17, and 19 were detectable at the protein level. By immunohisto- of Fgfr3 activation compared with wild-type chondrocytes and chemistry, FGF17 and 19 were uniformly expressed within the showed no receptor activation in the absence of ligand (8,9). growth plate. In contrast, FGF1 was found only in proliferating and hypertrophic chondrocytes whereas FGF2 localized predominantly to Despite the importance of the FGF ligand for activation of the resting and proliferating cartilage.
    [Show full text]
  • And Insulin-Like Growth Factor-I (IGF-I) in Regulating Human Erythropoiesis
    Leukemia (1998) 12, 371–381 1998 Stockton Press All rights reserved 0887-6924/98 $12.00 The role of insulin (INS) and insulin-like growth factor-I (IGF-I) in regulating human erythropoiesis. Studies in vitro under serum-free conditions – comparison to other cytokines and growth factors J Ratajczak, Q Zhang, E Pertusini, BS Wojczyk, MA Wasik and MZ Ratajczak Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA The role of insulin (INS), and insulin-like growth factor-I (IGF- has been difficult to assess. The fact that EpO alone fails to I) in the regulation of human erythropoiesis is not completely stimulate BFU-E in serum-free conditions, but does do in understood. To address this issue we employed several comp- lementary strategies including: serum free cloning of CD34؉ serum containing cultures indicates that serum contains some cells, RT-PCR, FACS analysis, and mRNA perturbation with oli- crucial growth factors necessary for the BFU-E development. godeoxynucleotides (ODN). In a serum-free culture model, both In previous studies from our laboratory, we examined the ؉ INS and IGF-I enhanced survival of CD34 cells, but neither of role of IGF-I12 and KL9,11,13 in the regulation of early human these growth factors stimulated their proliferation. The influ- erythropoiesis. Both of these growth factors are considered to ence of INS and IGF-I on erythroid colony development was be crucial for the BFU-E growth.3,6,8,14 Unexpectedly, that dependent on a combination of growth factors used for stimul- + ating BFU-E growth.
    [Show full text]
  • Factor-The Controlling Growth Factor-At a Sufficiently Low Value, While the Cell Population Has Ceased to Grow and Is in a Sort
    708 GENETICS: NO VICK A ND SZILARD PROC. N. A. S. EXPERIMENTS WITH THE CHEMOSTAT ON SPONTANEOUS MUTATIONS OF BACTERIA By AARON NOVICK AND LEO SZILARD INSTITUTRE OF RADIOBIOLOGY AND BIOPHYSICS, UNIVERSITY OF CHICAGO Communicated by H. J. Muller, October 18, 1950 Introduction.-All bacteria require for growth the presence of certain inorganic chemical components in the nutrient, such as potassium, phos- phorus, sulphur, etc., and with a few exceptions all bacteria require an energy-yielding carbon source, such as, for instance, glucose or lactate, etc. In addition to these elements or simple compounds, certain bacteria require more complex compounds, for instance an amino acid, which they are not capable of synthesizing. For the purposes of this presentation, any of the chemical compounds which a given strain of bacteria requires for its growth will be called a "growth factor." In general, the growth rate of a bacterial strain may be within very wide limits independent of the concentration of a given growth factor; but since at zero concentration the growth rate is zero, there must of necessity exist, at sufficiently low concentrations of the growth factor, a region in which the growth rate falls with falling concentration of the growth factor. It therefore should be possible to maintain a bacterial population over an indefinite period of time growing at a rate considerably lower than normal simply by maintaining the concentration of one growth factor-the controlling growth factor-at a sufficiently low value, while the concentrations of all other growth factors may at the same time be main- tained at high values.
    [Show full text]
  • Updated November 2019 KIT Mutation the KIT Gene Is Associated With
    Updated November 2019 KIT Mutation The KIT gene is associated with autosomal dominant piebaldism, gastrointestinal stromal tumors (GISTs), and familial mastocytosis.1-3 The type of mutation identified in the KIT gene determines the symptoms/cancer risks that the individual may have. Pathogenic loss-of-function (LOF) mutations in the KIT gene are generally only associated with Piebaldism. However, pathogenic gain of function mutations in the KIT gene are associated with systemic mastocytosis and gastrointestinal stromal tumors.4,5 Piebaldism: This is a rare condition characterized by the patchy absence of melanocytes in certain areas of the skin and hair. Melanocytes produce the pigment melanin, which contributes to hair, eye, and skin color. The absence of melanocytes leads to patches of skin and hair that are lighter than normal. These unpigmented areas are typically present at birth and do not increase in size or number.6-8 Gastrointestinal stromal tumors (GIST): GISTs are tumors that occur in the gastrointestinal tract, most commonly in the stomach or small intestine. Small tumors may cause no signs or symptoms. However, some people with GISTs may experience pain or swelling in the abdomen, nausea, vomiting, loss of appetite, or weight loss. These tumors can be cancerous (malignant) or noncancerous (benign). Mastocytosis: This is a blood disorder that occurs when white blood cells called mast cells accumulate in one or more tissues (most commonly in the bone marrow). Mast cells normally trigger inflammation during an allergic reaction. When an environmental trigger activates mast cells, they release proteins that signal an immune response. In systemic mastocytosis, excess mast cells mean more proteins are being released in the tissues where the cells accumulate, leading to an increased immune response.
    [Show full text]
  • B-Cell Growth Factor
    Proc. Natl Acad. Sci. USA Vol. 79, pp. 7455-7459, December 1982 Immunology B-cell growth factor: Distinction from T-cell growth factor and B-cell maturation factor (B lymphocytes/T-cell hybridomas) TOMAS LEANDERSON*, ERIK LUNDGRENt, ERIK RUUTH*, HAKAN BORGt, HAKAN PERSSONt, AND ANTONIO COUTINHOt tLaboratory for Cell and Tissue Culture Research, *Institute of Pathology, and +Department of Immunology, UmeA University, S-901 87 UmeA, Sweden Communicated by Niels K. Jerne, July 12, 1982 ABSTRACT A T-cell hybridoma was derived by somatic cell We have initiated these studies by systematically screening hybridization between concanavalin A-activated BALB/c spleen hybridoma activities in different assays that measure either cells and the AKR thymoma BW 5147. Media conditioned by hy- growth or maturation ofall B lymphocytes, regardless ofclonal bridoma cells, even at high dilutions (1:1,000) support the growth specificity. We chose to study activated rather than resting B oflipopolysaccharide-stimulated B-cell blasts but not that ofT-cell cells because of the-overwhelming evidence that initiation of growth factor (TCGF)-reactive T-cells. This activity, herein des- cooperative B-cell responses requires direct cellular interaction ignated B-cell growth factor (BCGF), has a Mr of =20,000 and it (13, 14). In this report we describe a hybridoma clone secreting can readily be separated from TCGF (Mr "30,000) by gel filtra- a factor that supports growth but not maturation of lipopoly- tion. BCGF is constitutively produced by the hybridoma cells, it saccharide (LPS)-activated B-cell blasts and is devoid of is removed from conditioned media by incubation with target cells T-cell at +4°C, and it is equally effective on B-cell blasts carrying dif- growthfactor(TCGF) andT-cellreplacingfactor(TRF) activities.
    [Show full text]
  • Human Transforming Growth Factor Α (TGF-Α)
    787 STOMACH Gut: first published as 10.1136/gut.51.6.787 on 1 December 2002. Downloaded from Human transforming growth factor α (TGF-α) is digested to a smaller (1–43), less biologically active, form in acidic gastric juice T Marchbank, R Boulton, H Hansen, R J Playford ............................................................................................................................. Gut 2002;51:787–792 Background: Transforming growth factor α (TGF-α) is a 50 amino acid peptide with potent prolifera- tive and cytoprotective activity present in gastric mucosa and juice. Aims: To determine the forms and biological activity of natural and recombinant TGF-α following incu- bation with acid pepsin. Patients: Human gastric juice was obtained under basal conditions from patients taking acid suppres- sants and from volunteers undergoing intragastric neutralisation. See end of article for Methods: Samples were analysed using mass spectroscopy and/or high pressure liquid chromatogra- authors’ affiliations phy with radioimmunoassay. Biological activity was determined using thymidine incorporation into rat ....................... hepatocytes and an indomethacin/restraint induced gastric damage rat model. α α Correspondence to: Results: TGF- 1–50 is cleaved to TGF- 1–43 by acid pepsin and this is the predominant form in normal Professor R J Playford, gastric juice. However, intragastric neutralisation or taking acid suppressants caused the predominant Gastroenterology Section, α α 3 form to be TGF- 1–50.TGF- 1–43 had only half of the ability to maximally stimulate [ H]thymidine incorpo- Imperial College School of α ration into primary rat hepatocytes (28 177 (1130) DPM/well for 2.16 nM TGF- 1–43 v 63 184 (3536) Medicine, Hammersmith α Hospital Campus, Du Cane DPM/well for TGF- 1–50; p<0.001).
    [Show full text]
  • Insulin As a Growth Factor
    003 1-3998/85/1909-0879$02.00/0 PEDIATRIC RESEARCH Vol. 19, No. 9, 1985 Copyright O 1985 International Pediatric Research Foundation, Inc Printed in U.S. A. Insulin as a Growth Factor D. J. HILL AND R. D. G. MILNER Departrnenl c!j'Pucdiutricc, Unive,:sitj. of Sl~effield,Cliildren :s Hospital, Shefic~ld,England ABSTRACT. Insulin is a potent mitogen for many cell attention than its well known, acute metabolic actions. Insulin types in vitro. During tissue culture, supraphysiological also can influence growth in vivo. The poor growth of a chilld concentrations of insulin are necessary to promote cell with diabetes (1) contrasts with the overgrowth of the hyperin- replication in connective or musculoskeletal tissues. Insulin sulinemic infant of a diabetic mother (2). The growth-promoting promotes the growth of these cells by binding, with low effect of insulin in vivo was demonstrated experimentally by affinity, to the type I insulin-like growth factor (IGF) Salter and Best in 1953 (3); these investigators restored growth receptor, not through the high affinity insulin receptor. In to hypophysectomized rats by treatment with insulin and a high other cell types, such as hepatocytes, embryonal carcinoma carbohydrate diet. Rats given insulin grew as well as those given cells, or mammary tumor cells, the type I IGF receptor is growth hormone but consumed substantially more food. Any virtually absent, and insulin stimulates the growth of these analysis of the action of insulin in promoting growth must clearly cells at physiological concentrations by binding to the high separate those effects which are due to anabolism resulting frc~m affinity insulin receptor.
    [Show full text]
  • Natural Human Interleukin-2 (IL-2) / T-Cell Growth Factor (TCGF) Catalog #: 0801017
    Natural Human Interleukin-2 (IL-2) / T-Cell Growth Factor (TCGF) Catalog #: 0801017 PRODUCT CHARACTERISTICS CONTENTS Each lot of IL-2/TCGF is analyzed for its ability to Each 50 ml bottle contains approximately 25,000 stimulate proliferation of seven to ten day old BRMP (Biological Response Modifier Program) PHA-transformed human T- lymphocytes. When units of IL-2/TCGF at a concentration of used at a final concentration of approximately approximately 500 BRMP units/ml. It is supplied 10% (v/v), IL-2/TCGF will induce a minimum five- as a sterile solution in 25mM HEPES buffered fold increase in cell concentration of such cells RPMI 1640 culture medium which is free of when seeded at a density of 2.0 x 105/ ml. IL- serum. IL-2/TCGF protein concentration ranges 2/TCGF, a glycoprotein with a molecular weight from 8-22 µg/ml. of approximately 15,000 kDa, is produced from pooled human PHA-stimulated T-lymphocytes. IL-2/TCGF is purified by several chromatographic STORAGE AND STABILITY steps to remove PHA and interferon. The solution is stable at -10ºC or below. The material may be re-frozen after thawing. RECOMMENDED USAGE Repetitive freezing and thawing is not recommended (aliquot if necessary). Thawed This preparation will support growth of most IL- material may be stored at 4 C for short-term 2/TCGF dependent lymphocyte cultures at a final usage. concentration of 10% (v/v). The initial cell density should be approximately 2.0 x 105/ ml and the cell culture should be adjusted to this density CAUTION: DO NOT USE IN HUMANS.
    [Show full text]
  • Transforming Growth Factor-β in Stem Cells and Tissue Homeostasis
    Bone Research www.nature.com/boneres REVIEW ARTICLE OPEN Transforming growth factor-β in stem cells and tissue homeostasis Xin Xu1, Liwei Zheng2, Quan Yuan3, Gehua Zhen4, Janet L. Crane4,5, Xuedong Zhou1 and Xu Cao4 TGF-β 1–3 are unique multi-functional growth factors that are only expressed in mammals, and mainly secreted and stored as a latent complex in the extracellular matrix (ECM). The biological functions of TGF-β in adults can only be delivered after ligand activation, mostly in response to environmental perturbations. Although involved in multiple biological and pathological processes of the human body, the exact roles of TGF-β in maintaining stem cells and tissue homeostasis have not been well-documented until recent advances, which delineate their functions in a given context. Our recent findings, along with data reported by others, have clearly shown that temporal and spatial activation of TGF-β is involved in the recruitment of stem/progenitor cell participation in tissue regeneration/remodeling process, whereas sustained abnormalities in TGF-β ligand activation, regardless of genetic or environmental origin, will inevitably disrupt the normal physiology and lead to pathobiology of major diseases. Modulation of TGF- β signaling with different approaches has proven effective pre-clinically in the treatment of multiple pathologies such as sclerosis/ fibrosis, tumor metastasis, osteoarthritis, and immune disorders. Thus, further elucidation of the mechanisms by which TGF-β is activated in different tissues/organs and how targeted cells respond in a context-dependent way can likely be translated with clinical benefits in the management of a broad range of diseases with the involvement of TGF-β.
    [Show full text]
  • Erythropoietin and Growth Factors Exhibit Differential Angiogenic Potential in Mouse Heart
    in vivo 22 : 587-592 (2008) Erythropoietin and Growth Factors Exhibit Differential Angiogenic Potential in Mouse Heart OLGA PAGONOPOULOU 1, ANNA EFTHIMIADOU 1, MARIA LAMBROPOULOU 2, NIKOS PAPADOPOULOS 2 and NIKOS K. NIKOLETTOS 1 Departments of 1Physiology and 2Histology – Embryology, Democritus University of Thrace, Medical School, Alexandroupolis, 68100, Greece Abstract. Background: The possible angiogenic effect of functions of EPO, angiogenesis, the process through which recombinant human erythropoietin (rHuEpo) and several new blood vessels arise from preexisting ones, has been possibly angiogenic cytokines such as basic fibroblast growth indicated (5). The potential role of EPO in angiogenesis may factor (bFGF), acidic fibroblast growth factor (aFGF) and be considered as a subset of its possible function in improving vascular endothelial growth factor (VEGF) was investigated overall tissue oxygenation and its antiapoptotic role (5). in mouse heart. Materials and Methods: Mice were divided Recombinant human erythropoietin (rHuEPO) induces a into five groups (n=7/group): A, control; B, rHuEpo-treated; proangiogenic phenotype in human endothelial cells, including C, (aFGF-treated); D, (VEGF-treated); E, (bFGF-treated). both early ( i.e. increase in cell proliferation and matrix The antibody mouse anti-human CD31 was used to evaluate metalloproteinase-2 production) and late (differentiation into the vessels present in histological preparations. Results: The vascular tubes) angiogenic events. In vivo , in the chick embryo results show a significant increase of the vessel number per CAM assay, the angiogenic activity of rHuEPO was similar to optical field in the rHuEpo-treated, the bFGF-treated and that exerted by basic fibroblast growth factor (bFGF), a well- the VEGF-treated animals compared to controls whereas known angiogenic cytokine and endothelial cells of the CAM aFGF did not show a ny significant angiogenic activity.
    [Show full text]
  • Immunocytochemical Study of Transforming Growth Factor Expression in Benign and Malignant Gliomas
    AmericanJournal ofPathology, Vol. 134, No. 4, April 1989 Copyright © American Association ofPathologists Immunocytochemical Study of Transforming Growth Factor Expression in Benign and Malignant Gliomas V. Samuels, J. M. Barrett, S. Bockman, between oncogenes and growth factors has been estab- C. G. Pantazis, and M. B. Allen, Jr. lished for many polypeptide growth factors.4 The autono- From the Departments ofNeurosurgery, Anatomy, and mous production of growth factors has led to the auto- Pathology Medical College ofGeorgia, Augusta, Georgia, crine mechanism for neoplastic conversion. and CoreSCAN, Santa Rosa, California To obtain a more thorough understanding of the ubiq- uitous patterns of polypeptide growth factor production, we performed an immunocytochemical analysis in 20 Immunocytochemical studies usingpolyclonal an- cases of benign and malignant human glial tumors. Glial tibodies to epidermal growth factor (EGF) and tumors were chosen as a model of study because of their transforming growthfactor (TGF) alpha and beta precise morphologic correlation relating to malignant phe- wereperformed on 20 cases ofhuman gliomas. EGF notypes.5 immunoreactive material was detected in both be- nign and malignant glial tumors. In addition, EGF immunoreactive material was detected in normal Materials and Methods brain. TGF-beta was detected in both benign and malignant tumors, but was not detected in normal Twenty human glioma biopsies obtained from archival brain. In contrast, TGF-alpha was highly conserved cases within the Department of Pathology at the Medical in its expression, occurring predominantly in ma- College of Georgia from 1981 to 1984 were examined and lignant compared with benign or normal brain tis- classified by a neuropathologist. Of the 20 specimens, 8 sue (P < 0.0001).
    [Show full text]
  • Loss of Growth Factor Dependence and Conversion of Transforming Growth Factor-/?I Inhibition to Stimulation in Metastatic H-Ras-Transformed Murine Fibroblasts1
    (CANCER RESEARCH 48. 6999-7003. December IS, 1988] Loss of Growth Factor Dependence and Conversion of Transforming Growth Factor-/?i Inhibition to Stimulation in Metastatic H-ras-transformed Murine Fibroblasts1 Lois C. Schwarz, Marie-Claude Gingras,2 Grant Goldberg, Arnold H. Greenberg,3 and Jim A. Wright4 Manitoba institute of Cell Biology, and Departments of Biochemistry [L. C. S., J. A. W.] and Immunology [M. C. G., A. H. G.J, The University of Manitoba, 100 Olivia Street, Winnipeg, Manitoba K3E OV9, Canada ABSTRACT transfected cells are hypersensitive to TGF-0 (12), EGF, and bFGF (13). Furthermore, the effects of TGF-/3 on cells trans Cell lines with varying tumorigenic and metastatic potentials have been fected with the myc-oncogene are dependent on the entire set obtained by transformation of KIT1•¿fibroblastsusing radiation or trans- of GFs present at a given time (14). In contrast, ros-transfected fection with 1-24 H-ras. We have observed an inverse relationship cells can become insensitive to the effects of PDGF, EGF, between metastatic potential and dependence on serum for growth. The TGF-0 (12, 15), and bFGF (13), but are hypersensitive to the effects of basic fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, and transforming growth factor-/?i (TGF-0i) on effects of insulin-like GFs (13). It was postulated that ras- these lines were then examined to determine if the changes in the serum transfected cells secrete their own GFs leading to autocrine dependence of metastatic cells may be due to altered responsiveness to stimulation of growth (16).
    [Show full text]