Calumenin (4C6) Mouse mAb www.cellsignal.com - 20ºC Store at Support: 877-678-TECH (8324) [email protected]

100 µl (10 western blots) Orders: 877-616-CELL (2355) [email protected]

Entrez-Gene ID #813

#11991 rev. 01/05/15 UniProt ID #O43852

For Research Use Only. Not For Use In Diagnostic Procedures.

Applications Species Cross-Reactivity* Molecular Wt. Isotype Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% W, IF-IC H, M, R, Mk 47 kDa Mouse IgG1** Endogenous sodium azide. Store at –20°C. Do not aliquot the antibody. *Species cross-reactivity is determined by western blot. **Anti-mouse secondary antibodies must be used to Background: Calumenin belongs to the CREC family of detect this antibody. that also contains reticulocalbin, ERC-55/TCBP-49/ kDa Huh7 COS-7 C6 NIH/3T3 Recommended Antibody Dilutions: E6BP, Cab45, and crocalbin/CBP-50. These EF-hand proteins Western blotting 1:1000 localize to the (ER) and are involved in 200 140 Immunofluorescence (IF-IC) 1:50 the secretory pathway in mammalian cells (1). Calumenin exists 100 as two isoforms corresponding to alternativly spliced variants of 80 For product specific protocols and a complete listing the CALU (2). Calumenin has been shown to localize to the of recommended companion products please see the 60 ER via a carboxy terminal HDEF ER retention signal (3), and un- product web page at www.cellsignal.com 50 Calumenin dergoes secretion after trafficking through the secretory pathway 40 (4). Secreted calumenin plays a role in amyloidosis by interact- 30 ing with serum amyloid P component (5). Calumenin is also a chaperone for mutants of the cystic fibrosis transmembrane 20 conductance regulator (CFTR) (6). Researchers performing a proteomics analysis of endometrial cancer identified calumenin as a potential target for endometrial carcinoma (7). 10

Specificity/Sensitivity: Calumenin (4C6) Mouse mAb recog- Western blot analysis of extracts from various cell lines using nizes endogenous levels of total calumenin . Calumenin (4C6) Mouse mAb. Source/Purification: Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human calumenin protein. Background References: (1) Honoré, B. and Vorum, H. (2000) FEBS Lett 466, 11-8. (2) Jung, D.H. et al. (2006) Biochem Biophys Res Commun 343, 34-42. (3) Yabe, D. et al. (1997) J Biol Chem 272, 18232-9. (4) Vorum, H. et al. (1999) Exp Cell Res 248, 473-81. (5) Vorum, H. et al. (2000) FEBS Lett 465, 129-34. (6) Teng, L. et al. (2012) PLoS One 7, e40173. (7) Voisin, S.N. et al. (2011) PLoS One 6, e16352. Confocal immunofluorescent analysis of HeLa cells using Calumenin (4C6) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

DRAQ5 is a registered trademark of Biostatus Limited. DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Tween is a registered trademark of ICI Americas, Inc.

Thank you for your recent purchase. If you would like to provide a review visit www.cellsignal.com/comments. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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Applications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.