DOCSLIB.ORG

Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil a Food Science and Technology ISSN 0101-2061 DDOI http://dx.doi.org/10.1590/1678-457X.29116 Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity Huizhen LI1, Zhijun ZHANG1*, Dongliang HE1, Yaoyao XIA1, Qingye LIU1, Xiaojun LI1 Abstract Response surface methodology (RSM) was used to optimize ultrasound-assisted aqueous enzymatic extraction (UAAEE) conditions for perilla seed oil. Under the optimum conditions—which were a liquid-to-solid ratio of 4.4:1, a hydrolysis time of 2.66 h, a hydrolysis temperature of 50.87 °C and an ultrasound treatment time of 24.74 min—an oil yield of 31.34% was obtained. Comparisons of the physicochemical characteristics of oil obtained using UAAEE with those of oil obtained by solvent extraction (SE oil) and cold pressing extraction (CPE oil) revealed similar refractive indices and saponification values, but UAAEE oil had a higher iodine value and better stability against oxidation, with a low peroxide value. UAAEE oil contained higher levels of beneficial α-linolenic acid and phenolics than SE oil or CPE oil, and it also had superior efficiency in scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Keywords: perilla seed oil; ultrasound-assisted aqueous enzymatic extraction; physical and chemical characteristics; fatty acid composition; antioxidant activities. Practical Application: Based on its physical and chemical properties, perilla oil obtained using UAAEE would be suitable as high quality edible oil and could also have applications in the cosmetic and pharmaceutical industries. 1 Introduction Perilla (Perilla frutescens) is an annual herb that is widely transmission effect, which is mainly caused by cavitational effects used for cooking and medicinal purposes in several Asian (Chemat et al., 2011), could be used to enhance the extraction countries (Igarashi & Miyazaki, 2013), including China, efficiency of AEE for edible oils. Ultrasound-assisted extraction Japan and Korea. Perilla seed oil is rich in omega-3 fatty acids (UAE) has many advantages, including high extraction yields, (53.6%-64%), especially α-linolenic acid (ALA, 18:3, n-3, high reproducibility, low solvent use, short extraction times, low 52.58%-61.98%) (Zhou et al., 2014). ALA is metabolized to running costs, limited environment impact and easy adaptation long-chain polyunsaturated fatty acids (LC-PUFA) such as to industrial scale use (Vuong et al., 2014). eicosapentaenoic acid (EPA, 20:5, n-3) and docosahexaenoic acid (DHA, 22:6, n-3), which are especially important during In the present study, optimal conditions for ultrasound-assisted human brain development (Lee & Song, 2012). Because of its aqueous enzymatic extraction (UAAEE) of oil from perilla seeds high omega-3 fatty acid content, perilla seed oil also has the were investigated using response surface methodology (RSM). potential to lower the risk of many chronic diseases. It may The fatty acid composition, physicochemical properties and reduce inflammation, prevent abnormal clotting, relax blood antioxidant activities of perilla seed oil extracted by UAAEE were vessels, and have anticancer properties (Asif, 2011; Li et al., 2014). evaluated and compared with those of mechanically pressed oil and oil extracted using organic solvent. Perilla seed oil is typically obtained either by mechanical pressing or by extraction using organic solvents. However, the yield obtained by mechanical pressing is quite low and the use 2 Materials and methods of organic solvents causes unacceptable contamination of the 2.1 Plant materials and reagents environment and residues in the oil may be harmful to human health (Zhang et al., 2010). Aqueous enzymatic extraction P. frutescens seeds (oil content 37.8%) were collected in (AEE) has been successfully used for the extraction of oils from Dctober 2014 from the North University of China (Taiyuan, a variety of oil-bearing seeds, including olive (Najafian et al., Shanxi Province, China). The dried seeds were milled to 2009) and peanut (Jiang et al., 2010). Ultrasound has been approximately 250 µm and stored in airtight containers at 4 °C shown to accelerate heat and mass transfer and is a powerful until use. Chemical reagents were supplied by Beijing Chemical alternative to conventional extraction techniques. The strong mass Reagents Co. (Beijing, China). Received 21 Nov., 2016 Accepted 30 Apr., 2017 1School of Chemical Engineering and Environment, North University of China, Taiyuan, China *Corresponding author: [email protected] Food Sci. Technol, Campinas, 37(Suppl. 1): 71-77, Dec. 2017 71 Extraction and determination of perilla seed oils 2.2 Soxhlet extraction (SE) and cold pressing extraction and 0.5 M HCl. After the extraction process, the mixture was (CPE) centrifuged at 8000 g for 20 min at room temperature, giving three layers (oil, cream and skim). The uppermost oil layer was Perilla seeds (dried and milled, 5 g) were extracted with carefully removed using a micropipette. The oil yield per seed n-hexane (250 mL) in a Soxhlet extractor at 90 °C for 7 h. sample (100 g), on a dry-weight basis, was determined using The n-hexane was removed at 50 °C under reduced pressure the Equation 1: using a rotary evaporator (SHZ-95B, Yuhua Ltd., Gongyi, Henan, China). SE gave an extraction yield of 35.2% (w/w). An oil sample Oil yield (%) = ( weight of extracted oil/ weight of seeds)×100% (1) was also prepared by CPE. The oil samples were stored in glass vials at 4 °C prior to physical and chemical characterization. 2.4 RSM design and statistical analysis 2.3 Ultrasound-assisted aqueous enzymatic extraction Based on the results of a preliminary mono-factor test (data not shown), a Box-Behnken design (BBD) was used to determine (UAAEE) the effects of four independent variables, i.e., liquid/solid ratio X( 1), Powdered perilla seeds (15 g) were pretreated with water hydrolysis time (X2), hydrolysis temperature (X3) and ultrasound (water/seed ratio, 3:1 to 7:1 mL/g) in a tunable ultrasonic bath treatment time (X4), on perilla oil yield (Y). The independent (TH-400BQG, 50Hz; Tianhua Ultrasonic Electronic Equipment variables were coded at three levels (–1, 0, and 1). The complete Co., Ltd., Jining, Shandong, China) at 30 °C for 20 min-30 min at design consisted of 29 experimental points, including five a power of 400 W. An enzyme cocktail (cellulase (5.5%) / neutral replicates of the central points (all variables were coded as zero). proteinase (4.5%) / pectinase (7.5%)) was then added and A randomized experimental order was used to reduce the effect the mixture was incubated in a water bath at 45 °C-55 °C for of unexplained variability on the observed responses. The run 2 h-3 h with constant stirring. Before adding the enzymes, order, variable conditions, and experimental and predicted the pH of the mixture was adjusted to 7.0 using 0.5 M NaDH values are shown in Table 1. A regression analysis was carried Table 1. Box-Behnken design and observed responses of perilla oil yield using UAAEE. Hydrolysis Ultrasonic Dil yield (%) Liquid-solid ratio Hydrolysis ttime Run temperature time X (mL:g) X (h) Experimental 1 2 X (°C) X (min) predicted values 3 4 values 1 5:1 2.0 50 30 27.53 26.67 2 5:1 2.5 55 30 28.33 28.42 3 7:1 2.5 50 20 25.13 25.32 4 7:1 2.0 50 25 24.67 24.48 5 7:1 2.5 45 25 25.67 25.74 6 5:1 3.0 45 25 28.53 28.03 7 5:1 3.0 50 30 27.93 27.28 8 5:1 2.0 55 25 26.07 26.36 9 5:1 3.0 50 20 28.13 28.75 10 3:1 2.5 45 25 28.47 28.42 11 5:1 2.5 50 25 31.33 30.96 12 5:1 2.5 50 25 31.27 30.96 13 5:1 2.5 50 25 29.33 30.96 14 5:1 2.5 50 25 31.40 30.96 15 5:1 2.5 45 20 27.73 28.09 16 5:1 2.0 45 25 27.93 27.09 17 3:1 2.0 50 25 24.47 25.66 18 7:1 2.5 50 30 24.13 24.99 19 5:1 3.0 55 25 28.87 29.50 20 5:1 2.5 45 30 26.93 27.89 21 5:1 2.5 55 20 28.80 28.29 22 3:1 3.0 50 25 28.60 29.23 23 3:1 2.5 50 30 28.40 28.00 24 7:1 3.0 50 25 25.73 24.99 25 5:1 2.5 50 25 31.47 30.96 26 7:1 2.5 55 25 26.27 26.08 27 3:1 2.5 55 25 29.13 28.82 28 5:1 2.0 50 20 24.87 25.28 29 3:1 2.5 50 20 28.80 27.74 72 Food Sci. Technol, Campinas, 37(Suppl. 1): 71-77, Dec. 2017 Li et al. out to evaluate the response function as a quadratic polynomial 2.7 Determination of total tocopherols (TT) and total (Equation 2): phenolics (TP) 4 4 34 The TT contents of perilla seed oils were determined using 2 YXX=++ββ0 ∑i i ∑ β ii i + ∑∑ βijXX i j (2) the colorimetric method described by Wong et al. (1988). The TP i=11 i= i=1 ji = +1 contents were determined using the Folin-Ciocalteu method (Capannesi et al., 2000). where Y is the response variable (oil yield); β0 is the offset term, βi is the linear effect, iiβ is the squared effect, ijβ is the interaction 2.8 DPPH scavenging activity effect and Xi, and Xj are independent variables.
Load more

Recommended publications
  • Perilla Frutescens) and Sesame (Sesamum Indicum) Seeds
    foods Article Metabolite Profiling and Chemometric Study for the Discrimination Analyses of Geographic Origin of Perilla (Perilla frutescens) and Sesame (Sesamum indicum) Seeds 1, 1, 2 3 4 Tae Jin Kim y, Jeong Gon Park y, Hyun Young Kim , Sun-Hwa Ha , Bumkyu Lee , Sang Un Park 5 , Woo Duck Seo 2,* and Jae Kwang Kim 1,* 1 Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Korea; [email protected] (T.J.K.); [email protected] (J.G.P.) 2 Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju, Jeonbuk 55365, Korea; [email protected] 3 Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea; [email protected] 4 Department of Environment Science & Biotechnology, Jeonju University, Jeonju 55069, Korea; [email protected] 5 Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea; [email protected] * Correspondence: [email protected] (W.D.S.); [email protected] (J.K.K.); Tel.: +82-63-238-5305 (W.D.S.); +82-32-835-8241 (J.K.K.) These authors contributed equally to this work. y Received: 26 June 2020; Accepted: 21 July 2020; Published: 24 July 2020 Abstract: Perilla and sesame are traditional sources of edible oils in Asian and African countries. In addition, perilla and sesame seeds are rich sources of health-promoting compounds, such as fatty acids, tocopherols, phytosterols and policosanols. Thus, developing a method to determine the geographic origin of these seeds is important for ensuring authenticity, safety and traceability and to prevent cheating.
    [Show full text]
  • Herbs, Spices and Essential Oils
    Printed in Austria V.05-91153—March 2006—300 Herbs, spices and essential oils Post-harvest operations in developing countries UNITED NATIONS INDUSTRIAL DEVELOPMENT ORGANIZATION Vienna International Centre, P.O. Box 300, 1400 Vienna, Austria Telephone: (+43-1) 26026-0, Fax: (+43-1) 26926-69 UNITED NATIONS FOOD AND AGRICULTURE E-mail: [email protected], Internet: http://www.unido.org INDUSTRIAL DEVELOPMENT ORGANIZATION OF THE ORGANIZATION UNITED NATIONS © UNIDO and FAO 2005 — First published 2005 All rights reserved. Reproduction and dissemination of material in this information product for educational or other non-commercial purposes are authorized without any prior written permission from the copyright holders provided the source is fully acknowledged. Reproduction of material in this information product for resale or other commercial purposes is prohibited without written permission of the copyright holders. Applications for such permission should be addressed to: - the Director, Agro-Industries and Sectoral Support Branch, UNIDO, Vienna International Centre, P.O. Box 300, 1400 Vienna, Austria or by e-mail to [email protected] - the Chief, Publishing Management Service, Information Division, FAO, Viale delle Terme di Caracalla, 00100 Rome, Italy or by e-mail to [email protected] The designations employed and the presentation of material in this information product do not imply the expression of any opinion whatsoever on the part of the United Nations Industrial Development Organization or of the Food and Agriculture Organization of the United Nations concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.
    [Show full text]
  • Product Value Sheet Dietary Supplement
    Product Value Sheet Dietary Supplement D•I® Helps to Support a Balanced Digestive System* TARGETED BODY SYSTEMS: DIGESTIVE SYSTEM, VEGAN FRIENDLY* PE F Your digestive system is a complex maze of organs, functions, and metabolic RILLA LEA processes. These are affected by multiple elements such as: foods, physical or mental stress, hormone imbalance, lack of sleep or physical activity, age, or other issues which may contribute to digestive imbalance. The complex C H E IN AT synergistic botanical Digestive Blend found in D•I® contains perilla, Chinese red ESE RED D date, licorice, tangerine peel, ginger and Chinese quince. The digestive system receives and processes these wholesome food ingredients and uses them to support the function and balance of the full digestive tract. These ingredients L have been traditionally used for generations for supporting digestive health. ICORICE Daily, consistent use of D•I® helps to promote digestive health.* TA L N EE GERINE P GINGER C H E IN C ESE QUIN Primary Structure Function Support: 1. Supports digestive function* 2. Supports the stomach and gastrointestinal tract* 3. Supports overall health* Plant Fiber Capsules 100 Capsules | Code: 20030 **This product information is approved for USA Markets. E. EXCEL USA, LLC www.eexcel.net V.01 E. EXCEL North America – USA Product Value Sheet Dietary Supplement D•I® Helps to Support a Balanced Digestive System* THE SCIENCE BEHIND THE INGREDIENTS Perilla is a branching, tender plant with white to lavender flowers. When mature, the perilla plant grows to about four feet high. It is native to India, Burma, Japan, Korea and China.
    [Show full text]
  • Anti-Stress Effects of the Hydroalcoholic Extract of Rosa Gallica Officinalis in Mice
    Heliyon 5 (2019) e01945 Contents lists available at ScienceDirect Heliyon journal homepage: www.heliyon.com Anti-stress effects of the hydroalcoholic extract of Rosa gallica officinalis in mice Hiroshi Ueno a,*, Atsumi Shimada b, Shunsuke Suemitsu c, Shinji Murakami c, Naoya Kitamura c, Kenta Wani c, Yosuke Matsumoto d, Motoi Okamoto e, Yuko Fujiwara f, Takeshi Ishihara c a Department of Medical Technology, Kawasaki University of Medical Welfare, Okayama, 701-0193, Japan b Division of Food and Nutrition, Nakamura Gakuen University Junior College, 5-7-1 Befu, Jounan-ku, Fukuoka, 814-0198, Japan c Department of Psychiatry, Kawasaki Medical School, 577, Matsushima, Kurashiki, Okayama, 701-0192, Japan d Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8558, Japan e Department of Medical Technology, Graduate School of Health Sciences, Okayama University, Okayama, 700-8558, Japan f Department of Health and Sports Science, Kawasaki University of Medical Welfare, Okayama, 701-0193, Japan ARTICLE INFO ABSTRACT Keywords: Rosa gallica, a plant of the Rosa genus, has been used widely since the 13th century and is cultivated in many areas Neuroscience as a medicinal plant for the preparation of herbal medicines. However, details of the neuropsychological effects of Physiology R. gallica remain unclear; therefore we aimed to investigate the neuropsychological effects of a water-soluble extract of R. gallica in male C57BL/6N mice under normal conditions and under chronic stress. We adminis- tered a water-soluble extract of R. gallica to mice and performed a series of behavioral experiments to compare the treated animals with the untreated controls.
    [Show full text]
  • Title Oxalate Content in Common Japanese Foods Author(S)
    Title Oxalate content in common Japanese foods OGAWA, Yoshihide; TAKAHASHI, Shigeki; KITAGAWA, Author(s) Ryuichi Citation 泌尿器科紀要 (1984), 30(3): 305-310 Issue Date 1984-03 URL http://hdl.handle.net/2433/118142 Right Type Departmental Bulletin Paper Textversion publisher Kyoto University 305 Acta Urol. Jpn. Vol. 30l [ No.3, March 1984 ..J OXALATE CONTENT IN COMMON JAPANESE FOODS Yoshihide OGAWA, Shigeki TAKAHASHI and Ryuichi KITAGAWA From the Department of Urology, Juntendo University School of Medicine (Director.' Prof. R. Kitagawa, M.D.) The radioenzymatic method was used to determine the oxalate contents in common Ja­ panese foods. High concentrations of oxalate were detected in spinach, Perilla frutescens, pepper, sesame, chocolate, and tea. Beer and coffee also contained some oxalate. Fruit and meat were low in oxalate content. Therefore, among the common Japanese foods, the most important foods to be avoided to restrict oxalate intake are chocolate, tea, and spinach. Key words: Oxalate, Ca, Mg, P, Japanese foods are of interest in understanding stone­ INTRODUCTION formation and in treating stone-formers. Oxalate is well known to exert a very Therefore, we measured the oxalate, Ca, important effect on urinary-oxalate super­ Mg, and P in common Japanese foods. saturation. There are a variety of factors MATERIALS AND METHODS which influence the urinary concentration of oxalate. The quantity of oxalate in The standard solutions of Ca and Mg the diet is one factor; absorption increases for atomic absorption spectroscopy were urinary excretionl ,2). The calcium eCa) purchased from Kanto Chemicals, Ltd. present in food interferes with the absorp­ while the standard solution of P was tion of oxalate, and vice versa.
    [Show full text]
  • Perilla: Ancient Medicine
    Perilla: ancient medicine Perilla frutescens Perilla, Shiso, Wild basil, Wild red basil, Chinese basil, Purple mint, Rattlesnake weed, Summer coleus There are many scientifically proven medicinal uses for Perilla herb. It is a pungent, aromatic, warming herb. An infusion of the plant is useful in the treatment of asthma, colds, cough and lung afflictions, influenza prevention, nausea, vomiting, abdominal pain, constipation, food poisoning and allergic reactions (especially from seafood), and to restore health and balance. The stems are a traditional Chinese remedy for morning sickness and restless fetus in pregnancy, though some say the herb should be avoided by pregnant women. Perilla has been used for centuries in Oriental medicine as an anti-asthmatic, anti-bacterial, antidote, antimicrobial, anti-pyretic, antiseptic, antispasmodic, antitussive, aromatic, carminative, diaphoretic, emollient, expectorant, pectoral, restorative, stomachic and tonic. The plant constituents confirm these uses in alternative medicine and ongoing studies have revealed that this plant is useful in curing many cancers as well as various other diseases and disorders. Perilla Tea Recipe To 1 cup fresh herbs add 4 cups of boiling water, allow to steep 3 minutes. Add sweetener to taste. time, it was one of the main ingredients Perilla Herb Constituents in sarsaparilla. The entire plant is very Research has isolated such constituents nutritious, packed with vitamins and as apigenin, Ascorbic-acid, minerals, and one of the aldehyde beta-carotene, caffeic-acid, citral, isomers found in Perilla is 2,000 times dillapiol, elemicin, limonene, luteolin, as sweet as sugar. myristicin, perillaldehyde, protocatechuic-acid, quercetin, Other Uses for Perilla Plant rosmarinic-acid, and more, to0 Perilla seed oil has been used in paints, numerous to mention.
    [Show full text]
  • Asian Vegetables & Herbs Easy to Grow in Southern California
    Florence Nishida lagreengrounds.org March 2020 Asian Vegetables & Herbs Easy to Grow in Southern California Name Planting Need Pot Comments time support ok Legumes Snow Peas (Pisum sativum, var. macrocarpon Cool y/n y Bush and pole varieties ​ ​ ​ Spr/Fall Pea shoots – various Spr/Fall y y Pick young, not tendrils Soya Bean (Glycine max) Spr n n Pull up plant for dried ​ ​ Yard Long Bean (Vigna unguiculata ssp. Sesquipedalis) Warm y y Long harvest, keep picked ​ ​ ​ ​ spr Lab Lab Beans (Lab Lab purpureus, Dolichos lab lab), Warm y/n n Can be a perennial if no ​ ​ Hyacinth Bean spr, frost. Contains cyanogenic summer glycosides, eat young or boil mature beans. Winged Bean/Pea (Tetragonolobus purpureus) Cool n y Fast maturing, pretty ​ ​ Brassicas, Headed Chinese cabbage, Napa cabbage (Brassica rapa var. Late n n Fresh – mild sweet flavor, ​ ​ pekinensis) summer, sev. cultivars ​ fall Pak choi/ Bok choy (Brassica rapa var. chinensis), Spring n y Fast-grower, good for ​ ​ ​ ​ celery cabbage inter-cropping, harvest whole or lvs Rosette pak choi (Brassica chinensis var. narinosa), Mid n y Neat, compact, harvest ​ ​ ​ ​ ‘Tatsoi’, ‘Taisai’ summer, leaves or whole fall Flowering Stalk Brassicas Chinese broccoli (Brassica oleracea var. alboglabra) Late spr- n n Young flowering stems and ​ ​ ​ ​ ‘Gai lan’ fall buds Komatsuna (Brassica rapa var. komatsuna) Mustard Late n n Flavor bet. cabbage and ​ ​ ​ ​ spinach spr-fall mustard; eat whole or leaves Mustard Greens Mizuna (Brassica rapa var. japonica), green, red Fall- n y Mild flavored, pretty, pick ​ ​ ​ ​ varieties spring leaves Japanese Giant Red Mustard (Brassica juncea), Osaka Fall-Sprin n y/n Gorgeous, very spicy-hot ​ ​ Purple Mustard (B.
    [Show full text]
  • Perilla Shiso Leaves Stuffed with Rice and Pinenuts
    Purple Shiso - Perilla Perilla frutescens 'Crispa rubra' Height: 60cm plus (2ft) Suitable for: Rich well drained soil. Warm and sunny site. Summary: Purple Shiso is an annual herb that originates from S.E. Asia. It is reasonably hardy with purple crinkled leaves and pale pink flowers. Whilst it is extremely ornamental it is also brilliant to use in the kitchen. Delivery May onward (weather dependant). More information: Shiso can be planted outside in late spring, once the danger of frost has passed. It is similar in appearance to a tall purple coleus. The leaves are strongly scented and are reminiscent of cumin in flavour, the flavour increases in strength as the plant grows larger. It can grow quite tall in a warm sheltered position and it is a good thing to pinch out the tip of the plant at about 60cm, so that it bushes out. It makes a fine specimen plant in the centre of containers, or within planting schemes in the flower borders. Both the leaves and flowers can be eaten. The leaves are used as wraps in Japanese cuisine in a similar way to vine leaves in Greece, and they are given various fillings generally based on rice, chicken or pork. The leaves can also be used raw in salads and with raw fish, bean curd and dipped in batter to be cooked as tempura. The flowers make a pretty edible decoration to any dish. Shiso leaves stuffed with rice and pinenuts. 10 large shiso leaves 25g butter 1 onion – very finely chopped 3 tbls chives or lovage – chopped 125g cooled, cooked rice 1 beaten egg seasoning 40g pine nuts 1.
    [Show full text]
  • Growing Herbs for the Home Gardener
    Department of Horticultural Science Horticulture Information Leaflet 8110 2/98 GROWING HERBS FOR THE HOME GARDENER Erv Evans, Extension Associate, Horticultural Science Jeanine Davis, Extension Specialist, Horticultural Science An herb is any plant used whole or in part as • Plant herbs in average garden soil with an ingredient for health, flavor, or fragrance. organic matter added to improve texture Herbs can be used to make teas; perk up and drainage. cooked foods such as meats, vegetables, • Choose a site that receives at least 6 sauces, and soups; or to add flavor to hours of direct sun each day. vinegars, butters, dips, or mustards. Many herbs are grown for their fragrance and are • Avoid ground where water stands or used in potpourris, sachets, and nosegays; runs during heavy rains. or to scent bath water, candles, oils, or • Compensate for poor drainage with raised perfumes. More than 25% of our modern beds amended with compost. drugs contain plant extracts as active ingredients, and researchers continue to • Apply balanced fertilizers sparingly to isolate valuable new medicines from plants leafy, fast growing herbs. Heavy and confirm the benefits of those used in applications of fertilizer, especially those traditional folk medicine. containing large amounts of nitrogen, will decrease the concentration of essential Herbs as a group are relatively easy to grow. oils in the lush green growth. Begin your herb garden with the herbs you enjoy using the most. For example, choose Plan your herb garden by grouping herbs basil, oregano, and fennel for Italian cooking; according to light, irrigation, and soil lavender and lemon verbena for making requirements.
    [Show full text]
  • Perilla Frutescens
    Perilla frutescens Perilla frutescens Perilla Introduction Native to Eastern Asia, the genus Perilla contains only one species and three varieties. Perilla frutescens is well-known in Chinese medicine and has a long history of cultivation in China[172]. Species and Related Varieties of Perilla in China Scientific Name Perilla frutescens (L.) Britt. Perilla frutescens var. auriculato- Leaves and flowers of Perilla frutescens. dentata C.Y. Wu et Hsuan ex glandular dotted on the surface, and (Photo by Jil M. Swearingen, USDI-NPS.) H.W.Li pilose near the inside base, arranged December[172]. Perilla frutescens var. acuta (Thunb.) in a ring. The calyx is initially about Kudo 3 mm long, but expands to 1.1 cm, spreading or drooping and swollen at Habitat Perilla frutescens var. crispa (Thunb.) the base when fruited. The labiate calyx P. frutescens grows along roadsides, Hand.-Mazz. has a broad upper sepal that is three- ditches, forest margins, and on lobed and slightly longer than the lower hillsides. sepal, which has two lanceolate lobes. Distribution Taxonomy The corolla is campanulate, white to P. frutescens occurs naturally and is Family: Labiatae (Lamiaceae) magenta, 3-4 mm long, glabrous, and also cultivated in almost all provinces Genus: Perilla L. slightly pilose outside but less so inside. The limb of the corolla is also labiate, of China[172]. Description and composed of a slightly lobed upper Economic Importance Perilla frutescens is an erect, annual petal and three-lobed lower petal. The P. frutescens is widely cultivated as a herb that grows 0.3 to 2 m tall. The sub-globose fruits are reticulate nutlets, source of medicine and spices.
    [Show full text]
  • Beneficial Health Effects of Fennel Seeds (Shamar) on Male Rats
    Med. J. Cairo Univ., Vol. 80, No. 2, March: 101-113, 2012 www.medicaljournalofcairouniversity.com Beneficial Health Effects of Fennel Seeds (Shamar) on Male Rats Feeding High Fat-Diet AMR A. REZQ, Ph.D. The Department of Nutrition and Food Science, Faculty of Home Economics, Helwan University, Cairo, Egypt Abstract and low density lipoprotein cholesterol, and decrease the risk of chronic heart diseases by increasing level of HDL-C as Fennel has been known since antiquity as a medicinal well as enhancing activities of antioxidant enzymes. and aromatic herb, it commonly used in household remedy for various medicinal purposes. The present study was con- Key Words: Fennel – Hyperlipidemia – Hypercholestermia – ducted to investigate the effect of beneficial health effects of Liver functions – Antioxidant system. fennel seeds on feeding rats with high fat-diet. Histopatho- logical changes of aorta, heart and liver were examined. Thirty Introduction five male albino rats of Sprague-Dawley strain weighing 200±5g were used. Animals were divided into five groups of HYPERLIPIDEMIA is a heterogeneous disorder seven rats each: group (1) fed on the basal diet and kept as a negative control group; group (2) fed on high fat–diet and involving multiple etiologies. It is characterized kept as positive control group; groups (3), (4) and (5) fed on by the elevated in serum levels of free fatty acids, high fat diets supplemented with three different levels of triglycerides (TG), total lipid (TL), total cholesterol fennel seeds (5, 10 and 15%, respectively. The present results (TC), low-density lipoprotein-cholesterol (LDL- revealed that rats fed on high fat-diet only (positive control C) and Apolipoprotein B, and reduced serum high- group) had significant (at p<0.05) increase in serum TL, TG, TC, LDL-C, VLDL-C, AST, ALT, ALP and MDA concentra- density lipoprotein cholesterol (HDL-C) concen- tions, and significant decrease in serum HDL-C level as well tration [1] .
    [Show full text]
  • Extracts of Thai Perilla Frutescens Nutlets Attenuate Tumour Necrosis Factor-Α-Activated Generation of Microparticles, ICAM-1 and IL-6 in Human
    Bioscience Reports (2020) 40 BSR20192110 https://doi.org/10.1042/BSR20192110 Research Article Extracts of Thai Perilla frutescens nutlets attenuate tumour necrosis factor-α-activated generation of microparticles, ICAM-1 and IL-6 in human endothelial cells Downloaded from https://portlandpress.com/bioscirep/article-pdf/40/5/BSR20192110/882495/bsr-2019-2110.pdf by UK user on 15 June 2020 Narisara Paradee1, Niramon Utama-ang2, Chairat Uthaipibull3, John B. Porter4, Maciej W. Garbowski4 and Somdet Srichairatanakool1 1Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; 2Department of Product Development Technology, Faculty of Agro-industry, Chiang Mai University, Chiang Mai 50200, Thailand; 3National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand; 4Department of Haematology, University College London, Cancer Institute, London WC1E 6BT, United Kingdom Correspondence: Somdet Srichairatanakool ([email protected]) Elevation of endothelial microparticles (EMPs) play an important role in the progression of inflammation-related vascular diseases such as cardiovascular diseases (CVDs). Thai perilla (Perilla frutescens) nutlets are rich in phenolic compounds and flavonoids that exert potent antioxidant and anti-inflammatory effects. We found that the ethyl acetate (EA) and ethanol (Eth) extracts of Thai perilla nutlets contain phenolic compounds such as luteolin, apigenin, chryseoriol and their glycosides, which exhibit antioxidant activity. The goal of the present study was to investigate the effects of the extracts on endothelial activation and EMPs gener- ation in tumour necrosis factor-α (TNF-α)-induced EA.hy926 cells. We found that TNF-α (10 ng/ml) activated EA.hy926 cells and subsequently generated EMPs.
    [Show full text]