DOCSLIB.ORG

Long Ranger® 50% Gel Solutions ® Accugene TBE Buffers

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Long Ranger® 50% Gel Solutions ® Accugene TBE Buffers Lonza Rockland, Inc. www.lonza.com [email protected] Tech Service: 800-521-0390 Customer Service: 800-638-8174 Document # 18693-1007-05 Rockland, ME 04841 USA Long Ranger® 50% Gel Solutions ® AccuGENE TBE Buffers Complete Solutions for DNA Sequencing and Genotyping Specifications Let Lonza partner with you to meet your sequencing Components: 5X 10X and STR analysis research goals. We are committed 0.445 M Tris Base 0.89 M Tris Base to offering you a family of products and services 0.445 M Boric Acid 0.89 M Boric Acid designed to increase your throughput, efficiency and 0.01 M 0.02 M 2 2 2 2 quality. We continue to work with many world class Na EDTA• H2O Na EDTA• H2O genome facilities and core laboratories offering them 18 MΩ water 18 MΩ water the best products for their specific research needs Filtered: 2 µm 2 µm through our varied product line, technical support and Conductivity: 3.14-3.50 mS/cm 3.8-5.06 mS/cm application development. Allow us to contribute to your pH: 8.25-8.38 8.0-8.5 research success. Storage: 18°C-24°C 18°C-24°C Long Ranger® Gel Solution - Long Ranger® Gel Storage Conditions Solution is the core of our sequencing and genotyping TBE buffers should be stored at room temperature and product line. Due to its patented gel formulation, Long out of direct sunlight. TBE buffers are stable for up to 3 Ranger® gels produce longer, accurate reads. months from date of manufacture. AccuGENE TBE Buffer - Our buffer is prepared from the same qualified reagents used to produce our gel Preparing Diluted Solutions solutions. Liquid concentrates are quality tested to Dilute the liquid concentrates with deionized water. Do ensure lot-to-lot consistency. not store diluted solutions for longer than 24 hours. Precautions Buffer Preparation Handling and use of Long Ranger® Gel Solution are 10X TBE identical to handling conventional acrylamide solutions. Concentration grams/L Wear gloves and use all safety precautions required for 0.89 M Tris Base 108.0 g 0.89 M Boric Acid 55.0 g handling acrylamide solutions. Please read the material 2 safety data sheets (MSDS) prior to use. 0.02 M Na EDTA•2H20 7.44 g Storage Conditions Add deionized water to a final volume of 1L, mix Avoid storing solutions in direct sunlight. thoroughly and filter through ≤0.45 µm membrane. Storage Shelf Life NOTE: To obtain optimal results and prevent Temperature precipitation of TBE stock solution, do not allow any dust ® Long Ranger 50% Gel Solution Room 24 months particles to enter the container and pour from the bottle temperature ® rather than inserting a pipette. Make in ≤1 L amounts. AccuGENE 5X and 10X TBE Room 3 months Buffers temperature Do not store in carboy. Store at room temperature. Do not use if precipitate forms. ® AccuGENE 5X and 10X TBE Buffers ® Long Ranger 50% Gel Solution for Automated DNA Liquid Concentrate for Automated DNA Sequencing Sequencing Prepared from qualified reagents, which are used in ® Long Ranger 50% Gel Solution works with all Long Ranger®,AccuGENE® 5X and 10X TBE liquid automated sequencers and is compatible with both dye concentrates, are quality tested to ensure lot-to-lot ® terminator and dye primer chemistry. Long Ranger consistency. Supplied in a convenient container with a Gels offer uniform spacing and sharp peaks, resulting spigot. in long, high-accuracy read lengths. We typically 1 observe a 25% to 30% increase in read length and an increase in base calling accuracy on automated 6. Filter the solution through Whatman® #1 filter paper sequencers when comparing the Long Ranger® matrix (11mM) or a Nalgene® cellulose acetate filter (≤0.45 with standard polyacrylamide gels. µm). 7. If desired, degas the solution for 5 minutes. Storage Conditions 8. Add the appropriate amount of TEMED and APS. Mix Long Ranger® 50% Gel Solution is stable for 24 months gently. from the date of manufacture when stored at room temperature. Store solutions away from direct sunlight. Gel Casting The following steps must be completed without For Best Results delay. • Do not prepare and store premix solutions made from 1. Cast gel and insert comb according to your standard ® Long Ranger 50% Gel Solution. procedure. • Ensure the glass plates are thoroughly clean and 2. Once the gel is polymerized (approximately 30 dust free. Glass plates can accumulate fluorescent minutes), place paper towels soaked in contaminants from many sources including electrophoresis buffer over the ends of the plates and detergents, marker pens, ethanol and other solvents, then cover with plastic wrap. This will prevent poor quality water, and hands or gloves. These moisture loss as the polymerization process invisible contaminants can seriously affect data continues. collection. 3. Allow 2 hours for complete gel polymerization. • Once the gel is polymerized (approximately 30 minutes), place paper towels soaked in Preparing for Electrophoresis electrophoresis buffer over the ends of the plates and 1. Remove the comb and wash the plates as described then cover with plastic wrap. This will prevent in the ABI Automated Sequencer Manual. moisture loss as the polymerization process 2. Prepare a sufficient quantity of electrophoresis buffer continues. Wrapped gels may be stored at 4°C to fill both anodal and cathodal chambers by diluting overnight. 10X TBE stock with deionized water to 1X. • New instrument (matrix) files may need to be 3. Mount the gel cassette onto the sequencing prepared for ABI machines when changing reagents apparatus according to the manufacturer’s or dye sets. instructions. 4. To assure plates and gel are clean, perform the Plate Electrophoresis with the ABI Automated Check module specific to the dye set you are using. DNA Sequencers Gel Preparation 1. Assemble glass plates and spacers in the cassette following the method described in your automated sequencer instructions, or use the Owl Otter™ Sequencing Gel Caster (Model #SGC-1). 2. Mix the gel solution appropriate for your sequencer according to the formulations in the table below. 377-36 cm plates 377-48 cm plates 373-48 cm plates 373-34 cm plates 5% LR/6 M Urea 4.75% LR/6 M Urea 5% LR/8.3 M Urea 5.75% LR/7 M Urea Components 1X TBE 1X TBE 1X TBE 1X TBE Total Volume 50 ml 50 ml 100 ml 100 ml Urea 18.0 g 18.0 g 50.0 g 42.0 g dd Water 26 ml 26.25 ml 42 ml 46.5 ml Long Ranger® 5 ml 4.75 ml 10 ml 11.5 ml 50% 10X TBE 5 ml 5 ml 10 ml 10 ml Fresh 10% 250 µl 250 µl 500 µl 500 µl APS TEMED 35 µl 35 µl 70 µl 70 µl 3. Place the specified quantity of the first four components into a clean beaker and mix gently. 4. To facilitate dissolving the urea, the solution may be warmed, but cool the solution to room temperature prior to the addition of TEMED and APS. 5. If necessary, after the urea has dissolved, bring up to volume with deionized water. 2 ABI Prism® 377 with 36 and 48 cm Plates Electrophoresis with the LI-COR® Sequencing NOTE: Prepare an analysis matrix standard file for Long Ranger® Gel Solution as described in the ABI Systems Prism 377 Automated Sequencer Manual. Gel Preparation 1. Assemble glass plates and spacers in the cassette ® 1. Prepare sample sheet as normal using an following the method described in the Li-COR appropriate setting. Automated Sequencer Manual. 2. Pre-run the gel using the desired module until a 2. Mix the gel solution appropriate for your gel temperature of 51°C is achieved,about 10-20 concentration according to the formulation in the minutes. Do not pre-run longer than necessary to table below. reach 51°C. Gel Length 33 or 41 cm plates 3. Prepare DNA samples as you would for standard automated sequencing gels. Spacer Thickness 0.25 mm 0.2 mm 4. After pre-run is complete, pause the instrument ® 5.5% Long Gel Composition 6% Long Ranger ® and rinse the wells thoroughly with electrophoresis Ranger buffer. 7 M Urea/1.2X TBE 7 M Urea/1X TBE 5. Load samples as described in the ABI Automated Urea 25.2 g 25.2 g Sequencer Manual. ® 6. Run the gel using the desired module. Long Ranger 7. Analyze data as usual. 50% Gel Solution 7.2 ml 6.6 ml 10X TBE 7.2 ml 6.0 ml ABI 373A with 48 cm Plates dd Water (fill to) 60.0 ml 60.0 ml NOTE: Prepare an analysis matrix standard file for ® Long Ranger Gel Solution as described in the ABI 3. Place a 100 ml beaker with a stir bar on a balance 373A Automated Sequencer Manual. and tare the balance. 1. Set the run parameters to 48-cm run length, full scan 4. Measure 25.2 g of urea into the beaker. mode, 4.0% gel type, 31W power and 16-18h 5. Add the recommended amounts of acrylamide and collection time. Set the parameters for the data 10X TBE to the beaker. collection software as usual. 6. Add distilled-deionized water to a target weight of 2. Pre-run the gel for 10-20 minutes, then press “abort 67.5 grams (= 60 ml of gel solution). run” to stop the pre-run. Rinse the wells thoroughly 7. To facilitate the dissolving of urea, the solution may with electrophoresis buffer. be warmed. Prior to the addition of APS and TEMED, 3. Load samples as described in the ABI Automated cool to room temperature.
Load more

Recommended publications
  • Gel-Syn™ Product Information Caution
    GEL-SYN™ PRODUCT INFORMATION CAUTION: Federal law restricts this device to sale by or on the order of a physician (or properly licensed practitioner). CONTENT Each 1 mL of Gel-Syn contains: Sodium Hyaluronate: 8.4 mg Sodium Chloride: 8.5 mg Sodium Phosphate, Dibasic: 0.16 mg Sodium Phosphate, Monobasic: 0.045 mg Water for Injection: q.s. to 1.0 mL DESCRIPTION Gel-Syn is a sterile, buffered solution of highly purified sodium hyaluronate with a molecular weight of approximately 1100 kDa, obtained through fermentation of Streptococci of Lancefield groups A and C and chemically unmodified. INDICATION Gel-Syn is indicated for the treatment of pain in osteoarthritis (OA) of the knee in patients who have failed to respond adequately to conservative non-pharmacologic therapy and simple analgesics (e.g., acetaminophen). CONTRAINDICATIONS • Do not administer to patients with known hypersensitivity (allergy) to sodium hyaluronate preparations. • Do not inject Gel-Syn into the knees of patients having knee joint infections or skin diseases or infections in the area of the injection site. WARNINGS • Do not concomitantly use disinfectants containing quaternary ammonium salts for skin preparation because sodium hyaluronate can precipitate in their presence. • Inject into the synovial space only. Do not inject by intravascular route. • Do not inject outside the synovial space or into the synovial tissue or capsule. An extra- articular injection of the product can cause local adverse events. PRECAUTIONS General • The safety and effectiveness of Gel-Syn in locations other than the knee, and for conditions other than osteoarthritis, have not been established. • Strict aseptic administration technique must be followed.
    [Show full text]
  • Physical-Chemical Characteristics of Whitening Toothpaste and Evaluation of Its Effects on Enamel Roughness
    Dental materials Physical-chemical characteristics of whitening toothpaste and evaluation of its effects on enamel roughness Sérgio Paulo Hilgenberg(a) Abstract: This in vitro study evaluated the physical-chemical characteris- (a) Shelon Cristina Souza Pinto tics of whitening toothpastes and their effect on bovine enamel after ap- Paulo Vitor Farago(b) Fábio André Santos(a) plication of a bleaching agent (16% carbamide peroxide). Physical-chem- Denise Stadler Wambier(a) ical analysis was made considering mass loss by desiccation, ash content and pH of the toothpastes. Thirty bovine dental enamel fragments were prepared for roughness measurements. The samples were subjected to (a) Department of Dentistry, School of Dentistry, Ponta Grossa State University, bleaching treatments and simulated brushing: G1. Sorriso Dentes Brancos Ponta Grossa, PR, Brazil. (Conventional toothpaste), G2. Close-UP Whitening (Whitening tooth- (b) Department of Pharmacy, School of paste), and G3. Sensodyne Branqueador (Whitening toothpaste). The av- Dentistry, Ponta Grossa State University, erage roughness (Ra) was evaluated prior to the bleaching treatment and Ponta Grossa, PR, Brazil. after brushing. The results revealed differences in the physical-chemical characteristics of the toothpastes (p < 0.0001). The final Ra had higher values (p < 0.05) following the procedures. The mean of the Ra did not show significant differences, considering toothpaste groups and bleach- ing treatment. Interaction (toothpaste and bleaching treatment) showed significant difference
    [Show full text]
  • An Introduction to Fast Dissolving Oral Thin Film Drug Delivery Systems: a Review
    Muthadi Radhika Reddy /J. Pharm. Sci. & Res. Vol. 12(7), 2020, 925-940 An Introduction to Fast Dissolving Oral Thin Film Drug Delivery Systems: A Review Muthadi Radhika Reddy1* 1School of pharmacy, Gurunanak Institute of Technical Campus, Hyderabad, Telangana, India and Department of Pharmacy, Gandhi Institute of Technology and Management University, Vizag, Andhra Pradesh, India INTRODUCTION 2. Useful in situations where rapid onset of action Fast dissolving drug delivery systems were first developed required such as in motion sickness, allergic attack, in the late 1970s as an alternative to conventional dosage coughing or asthma forms. These systems consist of solid dosage forms that 3. Has wide range of applications in pharmaceuticals, Rx disintegrate and dissolve quickly in the oral cavity without Prescriptions and OTC medications for treating pain, the need of water [1]. Fast dissolving drug delivery cough/cold, gastro-esophageal reflux disease,erectile systems include orally disintegrating tablets (ODTs) and dysfunction, sleep disorders, dietary supplements, etc oral thin films (OTFs). The Centre for Drug Evaluation [4] and Research (CDER) defines ODTs as,“a solid dosage 4. No water is required for the administration and hence form containing medicinal substances which disintegrates suitable during travelling rapidly, usually within a matter of seconds, when placed 5. Some drugs are absorbed from the mouth, pharynx upon the tongue” [2]. USFDA defines OTFs as, “a thin, and esophagus as the saliva passes down into the flexible, non-friable polymeric film strip containing one or stomach, enhancing bioavailability of drugs more dispersed active pharmaceutical ingredients which is 6. May offer improved bioavailability for poorly water intended to be placed on the tongue for rapid soluble drugs by offering large surface area as it disintegration or dissolution in the saliva prior to disintegrates and dissolves rapidly swallowing for delivery into the gastrointestinal tract” [3].
    [Show full text]
  • Acrylamide Polymerization — a Practical Approach
    electrophoresis tech note 1156 Acrylamide Polymerization — A Practical Approach Paul Menter, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Polyacrylamide Gel Polymerization Hercules, CA 94547 USA AcrylamideBis Polyacrylamide Introduction The unparalleled resolution and flexibility possible with CH2 CH + CH2 CH CH2 CH CH2 CH CH2 CH polyacrylamide gel electrophoresis (PAGE) has led to its CO CO CO CO CO widespread use for the separation of proteins and nucleic NH2 NH NH2 NH2 NH acids. Gel porosity can be varied over a wide range to meet CH2 CH2 specific separation requirements. Electrophoresis gels and NH NH NH NH buffers can be chosen to provide separation on the basis of CO 2 2 CO CO C O charge, size, or a combination of charge and size. CH2 CH CH2 CH CH2 CH CH2 CH The key to mastering this powerful technique lies in the polymerization process itself. By understanding the important Purity of Gel-Forming Reagents parameters, and following a few simple guidelines, the novice Acrylamide can become proficient and the experienced user can optimize Gel-forming reagents include the monomers, acrylamide and bis, separations even further. as well as the initiators, usually ammonium persulfate and TEMED or, occasionally, riboflavin and TEMED. On a molar This bulletin takes a practical approach to the preparation of basis, acrylamide is by far the most abundant component in the polyacrylamide gels. Its purpose is to provide the information monomer solution. As a result, acrylamide may be the primary required to achieve reproducible, controllable polymerization. source of interfering contaminants (Dirksen and Chrambach For those users interested only in the “bare essentials,” the 1972).
    [Show full text]
  • Sucralfate Enemas
    Sucralfate Enemas Sucralfate enemas are used to treat inflammation of the rectum (the lower part of the large intestine leading to the anus); most commonly this is for radiation proctitis; bowel inflammation following radiotherapy. This treatment can help treat bleeding due to the inflammation, and can be continued for up to 24 weeks; you can stop when the bleeding stops. This fact sheet explains how you can administer the enema yourself, using a syringe and catheter. The hospital or community pharmacy can provide the Sucralfate enema, and you will need to ask your GP or practise nurse to supply the equipment you will need. Your practise nurse can help you practice preparing the enema, and could help you with administering it at home, if you find this difficult. 1. Using the syringe provided, draw up 10mls of the Sucralfate Suspension (2g) and then draw up 10mls of warm water and mix. Ensure that any excess air is expelled from the syringe. 2. Attach the catheter provided to the syringe tip and lubricate the end of the catheter with lubricating gel provided. 3. Lie on your left side with both knees bent. This position will help the flow of liquid in to the rectum and aid enema retention. 4. Position a towel underneath yourself to catch any leakage of fluid once the enema has been administered. 5. With a steady pressure, gently insert the catheter to a depth of approximately 10cm into the rectum. 6. Press down on the barrel of the syringe slowly, using a steady and even pressure, until the entire contents of the syringe have been administered.
    [Show full text]
  • Colgate Blue Minty Gel Toothpaste This Industrial Safety Data Sheet Is Not Intended for Consumers and Does Not Address Consumer Use of the Product
    APJ Colgate Blue Minty Gel Toothpaste This industrial Safety Data Sheet is not intended for consumers and does not address consumer use of the product. For information regarding consumer applications of this product, refer to the product label. Version Revision Date: SDS Number: Date of last issue: - 1.0 23.11.2016 660000003786 Date of first issue: 23.11.2016 SECTION 1. PRODUCT AND COMPANY IDENTIFICATION Product name : Colgate Blue Minty Gel Toothpaste Product code : 200000015708 : B05408280003 Manufacturer or supplier's details Company : Colgate-Palmolive Co Colgate-Palmolive Central America Region Address : Colgate-Palmolive Pty Ltd., 345 George St, Sydney, New South Wales, Australia 2000. Telephone : Consumer affairs: - AU 1800 802 307 (Mon – Fri 9 - 5) Emergency telephone number : CHEMTREC Australia +(61)-290372994 Global-CHEMTREC- +1 703-741-5970 Telefax : +50250224239500 나.Recommended use of the chemical and restrictions on use Recommended use : A formulated dentifrice. SECTION 2. HAZARDS IDENTIFICATION GHS Classification Serious eye damage/eye irri- : Category 2A tation GHS label elements Hazard pictograms : Signal word : Warning Hazard statements : H319 Causes serious eye irritation. 1 / 11 APJ Colgate Blue Minty Gel Toothpaste This industrial Safety Data Sheet is not intended for consumers and does not address consumer use of the product. For information regarding consumer applications of this product, refer to the product label. Version Revision Date: SDS Number: Date of last issue: - 1.0 23.11.2016 660000003786 Date of first issue: 23.11.2016 Precautionary statements : Prevention: P264 Wash skin thoroughly after handling. P280 Wear eye protection/ face protection. Response: P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.
    [Show full text]
  • Injection of Stabilized Hyaluronic Acid-Based Gel of Non-Animal Origin for the Correction of Nasolabial Folds: Comparison with and Without Lidocaine
    Injection of Stabilized Hyaluronic Acid-Based Gel of Non-Animal Origin for the Correction of Nasolabial Folds: Comparison with and without Lidocaine à y à z PER HEDE´ N,PHD, DAN FAGRELL, MD, JAN JERNBECK, MD, RICHARD RYLANDER, MD, à z z ULF SAMUELSON,PHD, GABRIELLA SELLMAN, MD, AND BIRGIT STARK,PHD BACKGROUND The use of injectable hyaluronic acid–based gel of non-animal origin, manufactured using the patented NASHA technology, is well established in aesthetic facial procedures. OBJECTIVE To compare injection pain, dermal filler efficacy, and safety of NASHA-based gel with or without 0.3% lidocaine hydrochloride when administered to the nasolabial fold (NLF). METHODS Forty-three subjects seeking correction of NLFs, with moderate or severe wrinkle severity, were recruited for this split-face, double-blind, comparative study. NASHA-based gel, with or without lidocaine hydrochloride, was injected into the deep layer of the dermis and/or subcutis, of the NLF. Pain, efficacy, and safety assessments included a treatment preference question, a 100-mm visual analogue scale for pain, the Wrinkle Severity Rating Scale, and adverse event reporting. RESULTS Ninety percent of subjects (lower 95% confidence limit=76%) rated injection of NASHA- based gel with lidocaine as less painful than NASHA-based gel alone. Subjects and physicians reported no significant differences in dermal filler efficacy between the two treatments during the 12-month follow-up period. The safety profile was similar for both products. CONCLUSION NASHA-based gel with lidocaine provides a more comfortable treatment experience than NASHA-based gel alone, with equivalent efficacy and safety observed after 12 months of follow-up.
    [Show full text]
  • CLOSE up Red Gel Toothpaste
    CLOSE UPTM Red Gel Toothpaste Safety Data Sheet According To Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules And Regulations Revision Date: 04/21/2015 Date of issue: 04/21/2015 Supersedes Date: 02/25/2004 Version: 1.0 SECTION 1: IDENTIFICATION Product Identifier Product Form: Mixture Product Name: CLOSE UPTM Red Gel Toothpaste Intended Use of the Product Oral Care. Name, Address, and Telephone of the Responsible Party Company Church & Dwight 500 Charles Ewing Blvd Ewing Township, NJ 08628 T 1-800-524-1328 www.churchdwight.com Emergency Telephone Number Emergency Number : For Medical Emergency: 1-888-234-1828, For Chemical Emergency: 1-800-424-9300 (CHEMTREC) SECTION 2: HAZARDS IDENTIFICATION This product is labeled in accordance with regulations administered by the Consumer Product Safety Commission (CPSC). The use pattern and exposure in the workplace are generally not consistent with those experienced by consumers. The requirements of the Occupational Safety and Health Administration applicable to this SDS differ from the labeling requirements of the CPSC and, as a result, this SDS may contain additional health hazard information not pertinent to consumer use and not found on the product label. Classification of the Substance or Mixture Classification (GHS-US) Skin Sens. 1 H317 Full text of H-phrases: see section 16 Label Elements GHS-US Labeling Hazard Pictograms (GHS-US) : GHS07 Signal Word (GHS-US) : Warning Hazard Statements (GHS-US) : H317 - May cause an allergic skin reaction. Precautionary Statements (GHS-US) : P261 - Avoid breathing vapors, mist, or spray. P272 - Contaminated work clothing must not be allowed out of the workplace.
    [Show full text]
  • Barium Enema? Your Child’S Doctor Will Tell You If There Are Special Test to Get More Information
    9. After your child has had a bowel movement, the during the test. Bring along a book for your child to Your Child’s Care technologist will take a few more X-rays of the read during waiting periods. The Le Bonheur radiology staff wants to care large intestine without the barium. For adolescents, privacy may be their biggest for your child’s physical and emotional needs. The 10. A doctor trained in reading X-rays will study concern. Explain the test to your teen, and encourage information in this brochure will answer questions you the pictures and send the results to your him/her to ask us questions. or your child may have about the scheduled barium child’s doctor. enema, and help prepare your child for the test. Does my child have to do anything Your child’s doctor can also answer any questions How do I tell my child about the different before the test? you may have, or you can call the Le Bonheur radiology department where your child will have the barium enema? Your child’s doctor will tell you if there are special test to get more information. Please talk to your child before you come to Le things to do before your child has the test. Please call Bonheur. It will make the procedure easier for you Radiology at 901-287-7041 if you need further What is a barium enema? and your child. Assure your child that any discomfort instructions. will only last a few minutes, and that the test will A barium enema helps the doctor see how the If your child is scheduled for an air contrast barium, not hurt.
    [Show full text]
  • Formulation of Topical Bioadhesive Gel of Aceclofenac Using 3-Level Factorial Design
    Archive of SID Iranian Journal of Pharmaceutical Research (2011), 10 (3): 435-445 Copyright © 2011 by School of Pharmacy Received: September 2009 Shaheed Beheshti University of Medical Sciences and Health Services Accepted: February 2010 Original Article Formulation of Topical Bioadhesive Gel of Aceclofenac Using 3-Level Factorial Design Sanjay Singh*, Rabinarayan Parhi and Anuj Garg Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi, 221005, India. Abstract The objective of this work was to develop bioadhesive topical gel of Aceclofenac with the help of response-surface approach. Experiments were performed according to a 3-level factorial design to evaluate the effects of two independent variables [amount of Poloxamer 407 (PL-407 = X1) and hydroxypropylmethyl cellulose K100 M (HPMC = X2)] on the bioadhesive character of gel, rheological property of gel (consistency index), and in-vitro drug release. The best model was selected to fit the data. Mathematical equation was generated by Design Expert® software for the model which assists in determining the effect of independent variables. Response surface plots were also generated by the software for analyzing effect of the independent variables on the response. Quadratic model was found to be the best for all the responses. Both independent variable (X1 and X2) were found to have synergistic effect on bioadhesion (Y1) but the effect of HPMC was more pronounced than PL-407. Consistency index was enhanced by increasing the level of both independent variables. An antagonistic effect of both independent variables was found on cumulative percentage release of drug in 2 (Y3) and 8 h (Y4). Both independent variables approximately equally contributed the antagonistic effect on Y3 whereas antagonistic effect of HPMC was more pronounced than PL-407.
    [Show full text]
  • The Use of Agar As a Solvent Gel in Objects Conservation
    Article: The use of agar as a solvent gel in objects conservation Author(s): Cindy Lee Scott Source: Objects Specialty Group Postprints, Volume Nineteen, 2012 Pages: 71-83 Compilers: Mina Thompson, Emily Hamilton, and Kari Dodson th © 2012 by The American Institute for Conservation of Historic & Artistic Works, 1156 15 Street NW, Suite 320, Washington, DC 20005. (202) 452-9545 www.conservation-us.org Under a licensing agreement, individual authors retain copyright to their work and extend publications rights to the American Institute for Conservation. Objects Specialty Group Postprints is published annually by the Objects Specialty Group (OSG) of the American Institute for Conservation of Historic & Artistic Works (AIC). A membership benefit of the Objects Specialty Group, Objects Specialty Group Postprints is mainly comprised of papers presented at OSG sessions at AIC Annual Meetings and is intended to inform and educate conservation-related disciplines. Papers presented in Objects Specialty Group Postprints, Volume Nineteen, 2012 have been edited for clarity and content but have not undergone a formal process of peer review. This publication is primarily intended for the members of the Objects Specialty Group of the American Institute for Conservation of Historic & Artistic Works. Responsibility for the methods and materials described herein rests solely with the authors, whose articles should not be considered official statements of the OSG or the AIC. The OSG is an approved division of the AIC but does not necessarily represent the AIC policy or opinions. THe Use OF AGAr As A sOlvenT Gel In OBJeCTs CONSERVATION CINDY LEE SCOTT ABSTRACT Agar, or agarose, is a rigid polysaccharide gel that has found use in conservation cleaning treatments of three-dimensional porous objects in recent years, most notably by Italian conservation scientists Marilena Anzani and Paulo Cremonesi.
    [Show full text]
  • Comparative Study of Enema Retention and Preference in Ulcerative Colitis J R Ingram, J Rhodes, B K Evans, R G Newcombe, G a O Thomas
    594 Postgrad Med J: first published as 10.1136/pgmj.2004.031690 on 2 September 2005. Downloaded from ORIGINAL ARTICLE Comparative study of enema retention and preference in ulcerative colitis J R Ingram, J Rhodes, B K Evans, R G Newcombe, G A O Thomas ............................................................................................................................... Postgrad Med J 2005;81:594–598. doi: 10.1136/pgmj.2004.031690 Background: Therapeutic enemas are often used to treat active colitis but their retention may be limited because of urgency to defecate. Some preparations may be better retained and tolerated than others See end of article for because of their physical properties. authors’ affiliations ....................... Aim: To compare patient preference and retention of four therapeutic enemas, including a nicotine enema, in patients with ulcerative colitis (UC). Correspondence to: Methods: Twenty four patients with active UC received the four trial enemas—corticosteroid, 5-amino Dr G A O Thomas, Department of salicylate (5-ASA), and nicotine liquid enemas and a corticosteroid foam, in a randomised order, taking Gastroenterology, one enema on each of four successive nights. Patients scored them 1 to 4 for ease of administration and University Hospital of retention, degree of abdominal bloating, and for their overall preference. Wales, Heath Park, Cardiff CF14 4XW, UK; Gareth. Results: Fifteen patients rated nicotine their overall favourite or second favourite, compared with 14 for [email protected]. corticosteroid foam and 11 for 5-ASA and corticosteroid liquids, but this was not significant (p = 0.302). nhs.uk Overall, there was no significant difference in overnight retention. However, the nicotine enema tended to be less well retained in patients with milder urgency but a higher proportion retained it overnight with Submitted 16 December 2004 more severe urgency (p = 0.031 compared with 5-ASA enema).
    [Show full text]