Ajay Kumar Tiwari Editor Advances in Seed Production and Management Advances in Seed Production and Management Ajay Kumar Tiwari Editor
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EFFECT of INDOL-3 ACETIC ACID on the BIOCHEMICAL PARAMETERS of Achoria Grisella HEMOLYMPH and Apanteles Galleriae LARVA
Pak. J. Biotechnol. Vol. 11 (2) 163-171 (2014) ISSN print: 1812-1837 www.pjbt.org ISSN Online: 2312-7791 EFFECT OF INDOL-3 ACETIC ACID ON THE BIOCHEMICAL PARAMETERS OF Achoria grisella HEMOLYMPH AND Apanteles galleriae LARVA Fevzi Uçkan, Havva Kübra Soydabaş* Rabia Özbek Kocaeli University, Faculty of Arts and Science, Department of Biology, 41380, Kocaeli, Turkey * E-mail : [email protected]; [email protected] Article received November 15, 2014, Revised December 12, 2014, Accepted December 18, 2014 ABSTRACT Biochemical structures such as lipid, protein, sugar and glycogen are known to play a pivotal role on the relationship between host and its parasitoid. Any changes in these parameters may have potential to alter the balance of the host-parasitoid relation. Taking this into account, the effects of plant growth regulator, indol-3 acetic acid (IAA) on the biochemical parameters of the host and parasitoid were investigated. Achoria grisella Fabricus (Lepidoptera: Pyralidae) is a serious pest and causes harmful impacts on honeycomb. Endoparasitoid Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) feeds on the hemolymph of the A. grisella larva and finally causes mortality of the host. Different concentrations (2, 5, 10, 50, 100, 200, 500, and 1,000ppm) of IAA were added to the synthetic diet of host larvae. Protein, lipid, sugar, and glycogen contents in hemolymph of host and in total parasitoid larvae were determined by Bradford, vanillin-phosphoric acid, and hot anthrone tests using UV-visible spectrophotometer, respectively. Protein level in host hemolymph increased upon supplement of each doses of IAA except for 10ppm. IAA application enhanced the level of sugar at 100 and 200ppm whereas a decrease was observed in lipid at 5, 10, 200, and 1,000ppm doses in host. -
Montreal Protocol on Substances That Deplete the Ozone Layer
MONTREAL PROTOCOL ON SUBSTANCES THAT DEPLETE THE OZONE LAYER 1994 Report of the Methyl Bromide Technical Options Committee 1995 Assessment UNEP 1994 Report of the Methyl Bromide Technical Options Committee 1995 Assessment Montreal Protocol On Substances that Deplete the Ozone Layer UNEP 1994 Report of the Methyl Bromide Technical Options Committee 1995 Assessment The text of this report is composed in Times Roman. Co-ordination: Jonathan Banks (Chair MBTOC) Composition and layout: Michelle Horan Reprinting: UNEP Nairobi, Ozone Secretariat Date: 30 November 1994 No copyright involved. Printed in Kenya; 1994. ISBN 92-807-1448-1 1994 Report of the Methyl Bromide Technical Options Committee for the 1995 Assessment of the MONTREAL PROTOCOL ON SUBSTANCES THAT DEPLETE THE OZONE LAYER pursuant to Article 6 of the Montreal Protocol; Decision IV/13 (1993) by the Parties to the Montreal Protocol Disclaimer The United Nations Environment Programme (UNEP), the Technology and Economics Assessment Panel co-chairs and members, the Technical and Economics Options Committees chairs and members and the companies and organisations that employ them do not endorse the performance, worker safety, or environmental acceptability of any of the technical options discussed. Every industrial operation requires consideration of worker safety and proper disposal of contaminants and waste products. Moreover, as work continues - including additional toxicity testing and evaluation - more information on health, environmental and safety effects of alternatives and replacements -
Risk of Exposure of a Selected Rural Population in South Poland to Allergenic Mites
Experimental and Applied Acarology https://doi.org/10.1007/s10493-019-00355-7 Risk of exposure of a selected rural population in South Poland to allergenic mites. Part II: acarofauna of farm buildings Krzysztof Solarz1 · Celina Pająk2 Received: 5 September 2018 / Accepted: 27 February 2019 © The Author(s) 2019 Abstract Exposure to mite allergens, especially from storage and dust mites, has been recognized as a risk factor for sensitization and allergy symptoms that could develop into asthma. The aim of this study was to investigate the occurrence of mites in debris and litter from selected farm buildings of the Małopolskie province, South Poland, with particular refer- ence to allergenic and/or parasitic species as a potential risk factor of diseases among farm- ers. Sixty samples of various materials (organic dust, litter, debris and residues) from farm buildings (cowsheds, barns, chaff-cutter buildings, pigsties and poultry houses) were sub- jected to acarological examination. The samples were collected in Lachowice and Kurów (Suski district, Małopolskie). A total of 16,719 mites were isolated including specimens from the cohort Astigmatina (27 species) which comprised species considered as allergenic (e.g., Acarus siro complex, Tyrophagus putrescentiae, Lepidoglyphus destructor, Glycy- phagus domesticus, Chortoglyphus arcuatus and Gymnoglyphus longior). Species of the families Acaridae (A. siro, A. farris and A. immobilis), Glycyphagidae (G. domesticus, L. destructor and L. michaeli) and Chortoglyphidae (C. arcuatus) have been found as numeri- cally dominant among astigmatid mites. The majority of mites were found in cowsheds (approx. 32%) and in pigsties (25.9%). The remaining mites were found in barns (19.6%), chaff-cutter buildings (13.9%) and poultry houses (8.8%). -
Serological Detection of Cardamom Mosaic Virus Infecting Small Cardamom, Elettaria Cardamomum L
Int. J. Life. Sci. Scienti. Res., 2(4): 333-338 (ISSN: 2455-1716) Impact Factor 2.4 JULY-2016 Research Article (Open access) Serological Detection of Cardamom Mosaic Virus Infecting Small Cardamom, Elettaria cardamomum L. Snigdha Tiwari 1*, Anitha Peter2, Shamprasad Phadnis3 1Ph. D Scholar, Department of Molecular Biology and Genetic Engineering, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarkhand, India 2Associate Professor, Department of Plant Biotechnology, UAS (B), GKVK, Bangalore, India 3Ph.D. Scholar, Department of Plant Biotechnology, UAS (B), GKVK, Bangalore, India *Address for Correspondence: Snigdha Tiwari, Ph. D Scholar, Department of Molecular Biology and Genetic Engineering, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarkhand, India Received: 20 April 2016/Revised: 19 May 2016/Accepted: 16 June 2016 ABSTRACT- Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were the world’s largest producer and exporter of cardamom till 1980. There has however been a reduction in production, mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA (DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid in using disease free material for better yields and hence increased profit to the farmer. -
Universidade Estadual De Campinas Instituto De Biologia
UNIVERSIDADE ESTADUAL DE CAMPINAS INSTITUTO DE BIOLOGIA Fabricio José Biasotto Francischini Morphological and molecular characterization of species of Diatraea ssp. (Lepidoptera: Crambidae) and elucidation of dispersal pattern in America continent Caracterização morfológica e molecular de espécies de Diatraea ssp. (Lepidoptera: Crambidae) e elucidação dos padrões de dispersão no continente americano CAMPINAS 2017 Fabricio José Biasotto Francischini Morphological and molecular characterization of species of Diatraea ssp. (Lepidoptera: Crambidae) and elucidation of dispersal pattern in America continent Caracterização morfológica e molecular de espécies de Diatraea ssp. (Lepidoptera: Crambidae) e elucidação dos padrões de dispersão no continente americano Thesis presented to the Institute of Biology of the University of Campinas in partial fulfillment of the requirements for the degree of Doctor in Genetics and Molecular Biology in the area of Plant Genetics and Genetic Breeding Tese apresentada ao Instituto de Biologia da Universidade Estadual de Campinas como parte dos requisitos exigidos para obtenção do título de Doutor em Genética e Biologia Molecular, na Área de Genética Vegetal e Melhoramento Orientadora: Profa. Dra. Maria Imaculada Zucchi Coorientador: Dr. Tederson Galvan ESTE ARQUIVO DIGITAL CORRESPONDE À VERSÃO FINAL DA TESE DEFENDIDA PELO ALUNO FABRICIO JOSÉ BIASOTTO FRANCISCHINI E ORIENTADO PELA PROFa. DRa. MARIA IMACULADA ZUCCHI CAMPINAS 2017 Campinas, 30 de agosto de 2017 COMISSÃO EXAMINADORA Profa. Dra.Maria Imaculada Zucchi (presidente) Prof. Dr. Thiago de Araújo Mastrangelo Prof. Dr. Pedro Takao Yamamoto Dr. Alessandro Alves Pereira Prof. Dr. Alberto Soares Corrêa Os membros da Comissão Examinadora acima assinaram a Ata de defesa, que se encontra no processo de vida acadêmica do aluno. Para Josy Minha eterna esposa Juntos recebemos o dom Da Graça! Constantemente você me ensina a construir nossa família alicerçados em Jesus. -
Quarantine Regulation for Importation of Plants
Quarantine Requirements for The Importation of Plants or Plant Products into The Republic of China Bureau of Animal and Plant Health Inspection and Quarantine Council of Agriculture Executive Yuan In case of any discrepancy between the Chinese text and the English translation thereof, the Chinese text shall govern. Updated November 26, 2009 更新日期:2009 年 12 月 16 日 - 1 - Quarantine Requirements for The Importation of Plants or Plant Products into The Republic of China A. Prohibited Plants or Plant Products Pursuant to Paragraph 1, Article 14, Plant Protection and Quarantine Act 1. List of prohibited plants or plant products, countries or districts of origin and the reasons for prohibition: Plants or Plant Products Countries or Districts of Origin Reasons for Prohibition 1. Entire or any part of the All countries and districts 1. Rice hoja blanca virus following living plants (Tenuivirus) (excluding seeds): 2. Rice dwarf virus (1) Brachiaria spp. (Phytoreovirus) (2) Echinochloa spp. 3. Rice stem nematode (3) Panicum spp. (Ditylenchus angustus (4) Paspalum spp. Butler) (5) Oryza spp., Leersia hexandra, Saccioleps interrupta (6) Rottboellia spp. (7) Triticum aestivum 2. Entire or any part of the Asia and Pacific Region West Indian sweet potato following living plants (1) Palau weevil (excluding seeds) (2) China (Euscepes postfasciatus (1) Calystegia spp. (3) Cook Islands Fairmaire) (2) Dioscorea japonica (4) Federated States of Micronesia (3) Ipomoea spp. (5) Fiji (4) Pharbitis spp. (6) Guam (7) Kiribati (8) New Caledonia (9) Norfolk Island -
Download The
9* PSEUDORECOMBINANTS OF CHERRY LEAF ROLL VIRUS by Stephen Michael Haber B.Sc. (Biochem.), University of British Columbia, 1975 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE STUDIES (The Department of Plant Science) We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA July, 1979 ©. Stephen Michael Haber, 1979 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the Head of my Department or by his representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of Plant Science The University of British Columbia 2075 Wesbrook Place Vancouver, Canada V6T 1W5 Date Jul- 27. 1Q7Q ABSTRACT Cherry leaf roll virus, as a nepovirus with a bipartite genome, can be genetically analysed by comparing the properties of distinct 'parental' strains and the pseudorecombinant isolates generated from them. In the present work, the elderberry (E) and rhubarb (R) strains were each purified and separated into their middle (M) and bottom (B) components by sucrose gradient centrifugation followed by near- equilibrium banding in cesium chloride. RNA was extracted from the the separated components by treatment with a dissociation buffer followed by sucrose gradient centrifugation. Extracted M-RNA of E-strain and B-RNA of R-strain were mixed and inoculated to a series of test plants as were M-RNA of R-strain and B-RNA of E-strain. -
Indian Meal Moth Plodia Interpunctella
Indian Meal Moth Plodia interpunctella Description QUICK SCAN Adults: Up to 13 mm (0.5 inches) long with wings that have copper brown tips. The part of the wings closest to the head is off white. SIZE / LENGTH Eggs: Oval, ivory in color and 2 mm (0.08 inches) long Adult 0.5 inch (13 mm) Larvae: Creamy white, brown head capsule. Coloration varies from Eggs 0.08 inch (2 mm) cream to light pink color, sometimes pale green. Pupae: Pupal cases are whitish with a yellow to brownish colored pupa COLOR RANGE inside. Adult Long wings with copper tips Larvae Creamy white, brown head Life Cycle Adult moths live for 10-14 days. Mated females can lay 200-400 eggs LIFE CYCLE singly or in groups. Eggs hatch in 3-5 days in warmer months and up to 7 days in cooler months. Larvae feed and become mature in 21 days Adults Live 10-14 days or as long as 30 days depending on food quality, temperature and Eggs Hatch 3-7 days humidity. Larvae will wander and pupation will occur away from infested materials. Adults emerge from the pupae in 7 to 10 days depending on temperature. FEEDING HABITS Damage and Detection Larvae Prefer: woolens, furs, and materials made with hair and Granular frass the size of ground pepper can be found in, on food feathers. materials such as nuts, dried fruits, cereals and processed foods containing nuts or seeds and made from wheat, rice or corn. The use of pheromone traps and inspections can determine location and degree of INFESTATION SIGNS infestation. -
Proceedings of a Workshop on Biodiversity Dynamics on La Réunion Island
PROCEEDINGS OF A WORKSHOP ON BIODIVERSITY DYNAMICS ON LA RÉUNION ISLAND ATELIER SUR LA DYNAMIQUE DE LA BIODIVERSITE A LA REUNION SAINT PIERRE – SAINT DENIS 29 NOVEMBER – 5 DECEMBER 2004 29 NOVEMBRE – 5 DECEMBRE 2004 T. Le Bourgeois Editors Stéphane Baret, CIRAD UMR C53 PVBMT, Réunion, France Mathieu Rouget, National Biodiversity Institute, South Africa Ingrid Nänni, National Biodiversity Institute, South Africa Thomas Le Bourgeois, CIRAD UMR C53 PVBMT, Réunion, France Workshop on Biodiversity dynamics on La Reunion Island - 29th Nov. to 5th Dec. 2004 WORKSHOP ON BIODIVERSITY DYNAMICS major issues: Genetics of cultivated plant ON LA RÉUNION ISLAND species, phytopathology, entomology and ecology. The research officer, Monique Rivier, at Potential for research and facilities are quite French Embassy in Pretoria, after visiting large. Training in biology attracts many La Réunion proposed to fund and support a students (50-100) in BSc at the University workshop on Biodiversity issues to develop (Sciences Faculty: 100 lecturers, 20 collaborations between La Réunion and Professors, 2,000 students). Funding for South African researchers. To initiate the graduate grants are available at a regional process, we decided to organise a first or national level. meeting in La Réunion, regrouping researchers from each country. The meeting Recent cooperation agreements (for was coordinated by Prof D. Strasberg and economy, research) have been signed Dr S. Baret (UMR CIRAD/La Réunion directly between La Réunion and South- University, France) and by Prof D. Africa, and former agreements exist with Richardson (from the Institute of Plant the surrounding Indian Ocean countries Conservation, Cape Town University, (Madagascar, Mauritius, Comoros, and South Africa) and Dr M. -
Tropical Insect Chemical Ecology - Edi A
TROPICAL BIOLOGY AND CONSERVATION MANAGEMENT – Vol.VII - Tropical Insect Chemical Ecology - Edi A. Malo TROPICAL INSECT CHEMICAL ECOLOGY Edi A. Malo Departamento de Entomología Tropical, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Tapachula, Chiapas, C.P. 30700. México. Keywords: Insects, Semiochemicals, Pheromones, Kairomones, Monitoring, Mass Trapping, Mating Disrupting. Contents 1. Introduction 2. Semiochemicals 2.1. Use of Semiochemicals 3. Pheromones 3.1. Lepidoptera Pheromones 3.2. Coleoptera Pheromones 3.3. Diptera Pheromones 3.4. Pheromones of Insects of Medical Importance 4. Kairomones 4.1. Coleoptera Kairomones 4.2. Diptera Kairomones 5. Synthesis 6. Concluding Remarks Acknowledgments Glossary Bibliography Biographical Sketch Summary In this chapter we describe the current state of tropical insect chemical ecology in Latin America with the aim of stimulating the use of this important tool for future generations of technicians and professionals workers in insect pest management. Sex pheromones of tropical insectsUNESCO that have been identified to– date EOLSS are mainly used for detection and population monitoring. Another strategy termed mating disruption, has been used in the control of the tomato pinworm, Keiferia lycopersicella, and the Guatemalan potato moth, Tecia solanivora. Research into other semiochemicals such as kairomones in tropical insects SAMPLErevealed evidence of their presence CHAPTERS in coleopterans. However, additional studies are necessary in order to confirm these laboratory results. In fruit flies, the isolation of potential attractants (kairomone) from Spondias mombin for Anastrepha obliqua was reported recently. The use of semiochemicals to control insect pests is advantageous in that it is safe for humans and the environment. The extensive use of these kinds of technologies could be very important in reducing the use of pesticides with the consequent reduction in the level of contamination caused by these products around the world. -
Galleria Mellonella L
USO DE Galleria mellonella L. (LEP.: PYRALIDAE) COMO PRESA CENTINELA PARA EVALUAR EL IMPACTO DE ENEMIGOS NATURALES SOBRE Diatraea tabernella DYAR (LEP.: CRAMBIDAE) EN CAÑA DE AZÚCAR EN PANAMÁ. Galleria mellonella L. (LEP.: PYRALIDAE) AS SENTINEL PREY TO EVALUATE THE IMPACT OF NATURAL ENEMIES OF Diatraea tabernella DYAR (LEP.: CRAMBIDAE) IN SUGARCANE IN PANAMA. Atencio V., 1Randy; Goebel, 2François-Régis; Guerra, 3Abby; Lopéz, 3Silvia 1Randy Atencio V. 1 Resumen: Para identificar los potenciales depredadores y [email protected] parasitoides de D. tabernella en caña de azúcar diferentes estadios Universidad de Montpellier, Francia de G. mellonella fueron utilizados como presa centinela durante 2 un periodo de 12 meses en campos de caña de azúcar en Panamá 2François-Régis Goebel para investigar una alternativa en la evaluación del impacto de [email protected] los enemigos naturales sobre el barrenador el tallo de la caña Centro de Cooperación Internacional en la de azúcar. Un total de 2233 artrópodos fueron capturados y Investigación Agronómica para el Desarrollo., Francia la familia predominante en las capturas fue Formicidae con 3Abby Guerra 3 6 especies colectadas. Las especies más abundantes fueron: [email protected] Solenopsis sp. (63%) y Camponotus spp. (15.6%). Las especies Compañía Azucarera La Estrella S.A., Panamá con el mayor impacto sobre los huevos, larvas y pupas de G. (+84.1% de depredación) fueron: ., 3 mellonella Linepithema sp 3Silvia Lopéz Camponotus spp. y Ectatomma sp. (Hymenoptera: Formicidae). [email protected] Existe un potencial uso de G. mellonella como presa centinela Compañía Azucarera La Estrella S.A., Panamá para estudiar entomofauna e identificar especies que tengan un rol supresivo sobre poblaciones del barrenador del tallo, especialmente Formicidae. -
Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria Mellonella (Lepidoptera: Pyralidae)
Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria mellonella (Lepidoptera: Pyralidae) ABSTRACT The greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae) is the most destructive pest of honey bee, Apis mellifera (Hymenoptera: Apidae), throughout the world. The present study was conducted to determine the quantitative and qualitative impairing effects of the arthropod venoms, viz., death stalker scorpion Leiurus quinquestriatus venom (SV), oriental Hornet (wasp) Vespa orientalis venom (WV) and Apitoxin of honey bee Apis mellifera (AP) on the larval haemogram. For this rd purpose, the 3 instar larvae were treated with LC50 of each of these venoms (3428.9, 2412.6, and 956.16 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five basic types of the freely circulating haemocytes in the haemolymph of last instar (7th) larvae of G. mellonella had been identified: Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). All venoms unexceptionally prohibited the larvae to produce normal hemocyte population (count). No certain trend of disturbance in the differential hemocyte counts of circulating hemocytes in larvae of G. mellonella after treatment with the arthropod venoms. Increasing or decreasing population of the circulating hemocytes seemed to depend on the potency of the venom, hemocyte type and the larval instar. In PRs of last instar larvae, some cytopathological features had been observed after treatment with AP or WV, but SV failed to cause cytopathological features. With regard to PLs, some cytopathological features had been observed after treatment with AP while both SV and WV failed to cause cytopathological features in this hemocyte type.