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Datasheet: AHP1888 Product Details Datasheet: AHP1888 Description: RABBIT ANTI MOUSE DACT1 Specificity: DACT1 Other names: DAPPER HOMOLOG 1 Format: Purified Product Type: Polyclonal Antibody Isotype: Polyclonal IgG Quantity: 0.1 mg Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Western Blotting Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Target Species Mouse Product Form Purified IgG - liquid Antiserum Preparation Antisera to mouse Dact1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Buffer Solution TRIS buffered saline. Preservative 0.02% Thiomersal Stabilisers Approx. Protein IgG concentration 1.0mg/ml Concentrations Immunogen A peptide corresponding to amino acids 373-386 within exon4 of mouse Dact1. External Database UniProt: Links Q8R4A3 Related reagents Entrez Gene: 59036 Dact1 Related reagents Page 1 of 3 Synonyms Thyex3 Specificity Rabbit anti Mouse DACT1 antibody recognizes mouse Dact1 (Dapper homolog 1), an intracellular protein and member of the dapper family, which acts as a positive regulator of the signalling pathways of Dishevelled 2 (DVL2), a cytoplasmic protein which plays a pivotal role in Wnt signalling during vertebrate development. Dact1 is involved in enhancement of the transcriptional activation of target genes of the Wnt signalling pathway, through its intracellular co-localization with DVL2 and inhibition of the degradation of CTNNB1/beta-catenin. Mice homozygous for mutations in Dact1, display malformations involving the spine, genitourinary system and distal digestive tract, which was traced to disrupted germ-layer morphogenesis at the primitive streak (Suriben et al. 2009). Western Blotting AHP1888 detects a band of approximately 110kDa in mouse uterine tissue References 1. Suriben, R. et al. (2009) Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. Nat Genet. 41 (9): 977-85. Storage Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life 18 months from date of despatch. Acknowledgements This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California. Health And Safety Material Safety Datasheet documentation available at: Information Material Safety Datasheet Documentation #10337 available at: https://www.bio-rad-antibodies.com/uploads/MSDS/10337.pdf Regulatory For research purposes only Related Products Recommended Secondary Antibodies Sheep Anti Rabbit IgG (STAR34...) FITC Sheep Anti Rabbit IgG (STAR35...) RPE Goat Anti Rabbit IgG (H/L) (STAR124...) HRP Goat Anti Rabbit IgG (Fc) (STAR121...) Biotin, FITC, HRP Sheep Anti Rabbit IgG (2AB02...) Biotin Sheep Anti Rabbit IgG (STAR36...) DyLight®488, DyLight®549, DyLight®649, DyLight®680, DyLight®800 Recommended Useful Reagents TidyBlot™ WESTERN BLOT DETECTION REAGENT:HRP (STAR209P) Page 2 of 3 North & South Tel: +1 800 265 7376 Worldwide Tel: +44 (0)1865 852 700 Europe Tel: +49 (0) 89 8090 95 21 America Fax: +1 919 878 3751 Fax: +44 (0)1865 852 739 Fax: +49 (0) 89 8090 95 50 Email: [email protected] Email: [email protected] Email: [email protected] 'M313708:180329' Printed on 12 Apr 2018 © 2018 Bio-Rad Laboratories Inc | Legal | Imprint Page 3 of 3.
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