Datasheet Inhibitors / Agonists / Screening Libraries A DRUG SCREENING EXPERT

Product Name : B4 Catalog Number : TN1151 CAS Number : 29106-51-2 Molecular Formula : Molecular Weight : 578.52

Description: Procyanidin B4 reveals significant antioxidant activity

Storage: 2 years -80°C in solvent; 3 years -20°C powder;

Receptor (IC50) Nrf2

In vitro Activity Aimed to isolate and purify the from lotus seed skin by acetone extraction and rotary evaporation, identify their chemical structures by HPLC-MS-MS and NMR, and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo. The results showed that PMR mainly contained oligomeric proanthocyanidins, especially dimeric (PB1), and Procyanidin B4. Although it had limited ability to directly scavenge radicals in vitro, PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells. Molecular data revealed that PB1, a major component in PMR, stabilized Nrf2 by inhibiting the ubiquitination of Nrf2, which led to subsequent activation of the Nrf2-ARE pathway, including the enhancements of Nrf2 nuclear translocation, Nrf2-ARE binding and ARE transcriptional activity. Moreover, the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR[1]

Reference 1. Lotus seed skin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway.Acta Biochim Biophys Sin (Shanghai). 2019 Jan 1;51(1):31-40. 2. Comparison of compounds of three Rubus species and their antioxidant activity.Drug Discov Ther. 2015 Dec;9(6):391-6.

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