DOCSLIB.ORG

The Effect of Hispidulin, a Flavonoid from Salvia Plebeia, on Human Nasopharyngeal Carcinoma CNE-2Z Cell Proliferation, Migration, Invasion, and Apoptosis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Effect of Hispidulin, a Flavonoid from Salvia Plebeia, on Human Nasopharyngeal Carcinoma CNE-2Z Cell Proliferation, Migration, Invasion, and Apoptosis molecules Article The Effect of Hispidulin, a Flavonoid from Salvia plebeia, on Human Nasopharyngeal Carcinoma CNE-2Z Cell Proliferation, Migration, Invasion, and Apoptosis Yiqun Dai 1,2, Xiaolong Sun 1, Bohan Li 1, Hui Ma 1, Pingping Wu 1, Yingping Zhang 1, Meilin Zhu 1, Hong-Mei Li 1, Minjian Qin 2,* and Cheng-Zhu Wu 1,* 1 School of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, China; [email protected] (Y.D.); [email protected] (X.S.); [email protected] (B.L.); [email protected] (H.M.); [email protected] (P.W.); [email protected] (Y.Z.); [email protected] (M.Z.); [email protected] (H.-M.L.) 2 Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China * Correspondence: [email protected] (M.Q.); [email protected] (C.-Z.W.); Tel.: +86-25-8618-5130 (M.Q.); +86-55-2317-5232 (C.-Z.W.) Abstract: Nasopharyngeal carcinoma (NPC) is a common malignant head and neck tumor. Drug resistance and distant metastasis are the predominant cause of treatment failure in NPC patients. Citation: Dai, Y.; Sun, X.; Li, B.; Ma, Hispidulin is a flavonoid extracted from the bioassay-guided separation of the EtOH extract of Salvia H.; Wu, P.; Zhang, Y.; Zhu, M.; Li, plebeia with strong anti-proliferative activity in nasopharyngeal carcinoma cells (CNE-2Z). In this H.-M.; Qin, M.; Wu, C.-Z. The Effect study, the effects of hispidulin on proliferation, invasion, migration, and apoptosis were investigated of Hispidulin, a Flavonoid from Salvia in CNE-2Z cells. The [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay plebeia, on Human Nasopharyngeal and the colony formation assay revealed that hispidulin could inhibit CNE-2Z cell proliferation. Carcinoma CNE-2Z Cell Proliferation, µ Migration, Invasion, and Apoptosis. Hispidulin (25, 50, 100 M) also induced apoptosis in a dose-dependent manner in CNE-2Z cells. Molecules 2021, 26, 1604. https:// The expression of Akt was reduced, and the expression of the ratio of Bax/Bcl-2 was increased. doi.org/10.3390/molecules26061604 In addition, scratch wound and transwell assays proved that hispidulin (6.25, 12.5, 25 µM) could inhibited the migration and invasion in CNE-2Z cells. The expressions of HIF-1α, MMP-9, and Academic Editors: Yasuyoshi Miyata, MMP-2 were decreased, while the MMPs inhibitor TIMP1 was enhanced by hispidulin. Moreover, Won Sup Lee, Dehai Li, hispidulin exhibited potent suppression tumor growth and low toxicity in CNE-2Z cancer-bearing Natthida Weerapreeyakul and mice at a dosage of 20 mg/kg/day. Thus, hispidulin appears to be a potentially effective agent for Kittisak Likhitwitayawuid NPC treatment. Received: 16 February 2021 Keywords: hispidulin; nasopharyngeal carcinoma; apoptosis; invasion; migration Accepted: 11 March 2021 Published: 14 March 2021 Publisher’s Note: MDPI stays neutral 1. Introduction with regard to jurisdictional claims in published maps and institutional affil- Nasopharyngeal carcinoma (NPC) is one of the most common head and neck ma- iations. lignant tumor. It has a very unique distribution pattern and is much more prevalent in southeast Asia [1,2]. The conventional treatment for patients with NPC is chemotherapy and radiotherapy. Although the treatments have revealed good efficacy, high recurrence and metastasis become the major cause of treatment failure and have a poor prognosis [3]. Therefore, it is necessary to look for more effective therapeutic drugs for treating NPC. Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Traditional Chinese herbal medicine has become an important source of antitumor This article is an open access article drugs and tumor adjuvant therapy with unique biological activities and few side effects. distributed under the terms and Salvia plebeia R. Brown is a traditional Chinese folk herb that belongs to the family Lamiaceae conditions of the Creative Commons and is abundant in many countries, especially in China and India. It has been used as Attribution (CC BY) license (https:// a folk herbal medicine for treating inflammatory diseases such as nephritis, pharyngitis, creativecommons.org/licenses/by/ bronchitis, colds, and coughs, etc. [4–6]. In addition, there are also reports suggesting 4.0/). that S. plebeia has antitumor activity [7,8]. Although clinical treatment of tumors and the Molecules 2021, 26, 1604. https://doi.org/10.3390/molecules26061604 https://www.mdpi.com/journal/molecules Molecules 2021, 26, x FOR PEER REVIEW 2 of 11 Molecules 2021, 26, 1604 2 of 11 that S. plebeia has antitumor activity [7,8]. Although clinical treatment of tumors and the antitumor poperties of S. plebeia extracts have been reported, no study has reported the bioassay-guided isolation of the active principle responsible for the antitumor activity. antitumorFlavonoids poperties are of theS. plebeiamain chemicalextracts haveconstituents been reported, of S. plebeia no study, which has has reported multiple the phar- bioassay-guidedmacological effects isolation and of low the toxicities. active principle Hispidulin responsible (4′,5,7-trihydroxy for the antitumor-6-methoxyflavone) activity. is a Flavonoidsflavonoid obtained are the main from chemical S. plebeia constituents and has several of S. plebeiapharmacological, which has multipleactivities phar-such as 0 macologicalantioxidative, effects anti and-inflammatory, low toxicities. anti Hispidulin-epileptic, (4hepatoprotective,5,7-trihydroxy-6-methoxyflavone) activities [9–12]. Note- is aworthy, flavonoid accumulating obtained from studiesS. plebeia have anddemonstrated has several the pharmacological anti-cancer activity activities of hispidulin such as in antioxidative,renal cell carcinoma, anti-inflammatory, gallbladder anti-epileptic, cancer, acute hepatoprotective myeloid leukemia, activities hepatocellular [9–12]. Note- carci- worthy,noma, accumulating and colorectal studies cancer have by causing demonstrated growth theinhibition, anti-cancer cell activitycycle arres of hispidulint, apoptotic in acti- renalvation, cell carcinoma, and metastasis gallbladder suppression cancer, [13]. acute However, myeloid leukemia,its anti-cancer hepatocellular activity on carcinoma, NPC remain andpoorly colorectal understood. cancer by Therefore, causing growthour study inhibition, aimed to cell explore cycle whether arrest, apoptotic hispidulin activation, is the main andeff metastasisective anti suppression-cancer component [13]. However, in S. plebeia its anti-cancerand investigate activityd the on anti NPC-cancer remain effects poorly of his- understood.pidulin on Therefore, NPC. our study aimed to explore whether hispidulin is the main effective anti-cancer component in S. plebeia and investigated the anti-cancer effects of hispidulin on2. NPC. Results 2. Results2.1. Isolation of Hispidulin from the Bioassay-Guided Fractionation of the EtOH Extract of S. 2.1.plebeia Isolation of Hispidulin from the Bioassay-Guided Fractionation of the EtOH Extract of S. plebeiaFirstly, we measured the cytotoxic effect of EtOH extract of S. plebeia on CNE-2Z cells. TheFirstly, crude we extract measured significantly the cytotoxic decreased effect ofCNE EtOH-2Z extract cell viability of S. plebeia (Figureon CNE-2Z1a). To look cells. for Thewhat crude components extract significantly are related decreased to the anti CNE-2Z-cancer celleffects viability of crude (Figure extract1a). of To S. look plebeia for, the whatcrude components extract was are suspended related to in the water anti-cancer and sequentially effects of partitioned crude extract with of petroleumS. plebeia, the ether, crudeCH extract2Cl2, ethyl was acetate suspended, and inn-butanol, water and respectively. sequentially The partitioned fractions withwere petroleum subjected ether,to a cell CHproliferation2Cl2, ethyl acetate, assay. andThe n-butanol,results showed respectively. that CH2 TheCl2 fraction fractions has were stronger subjected growth to a cellinhibi- proliferationtion on CNE assay.-2Z Thecells results than other showed fractions that CH (Figure2Cl2 fraction 1b). Based has on stronger this result, growth CH inhibition2Cl2 fraction onwas CNE-2Z chosen cells for than further other purification fractions to (Figure find the1b). active Based compound. on this result, High CH-performance2Cl2 fraction liquid waschromatography chosen for further (HPLC) purification analysis to findrevealed the active a major compound. peak in High-performancethe CH2Cl2 fraction liquid (Figure chromatography1c). The CH2Cl (HPLC)2 fraction analysis was chromatogr revealed aaphed major peakon a silica in the gel CH column2Cl2 fraction and Sephadex (Figure1c). LH- The20, CH and2Cl the2 fraction major compound was chromatographed was gained. The on a structure silica gel of column the compound and Sephadex was determined LH-20, andas the hispidulin major compound (Figure 1c) was by comparing gained. The the structure nuclear ofmagnetic the compound resonance was (NMR) determined data with as hispidulinprevious literature (Figure1 c)[14] by. The comparing purity of the hispidulin nuclear magnetic was higher resonance than 98% (NMR) by HPLC data analysis. with previous literature [14]. The purity of hispidulin was higher than 98% by HPLC analysis. Figure 1. Effects of EtOH extract and fractions from S. plebeia on cell viability in CNE-2Z cells. (a) CNE-2Z cells were Figure 1. Effects of EtOH extract and fractions from S. plebeia on cell viability
Load more

Recommended publications
  • Assessment Report on Salvia Officinalis L., Folium and Salvia Officinalis L., Aetheroleum Final
    20 September 2016 EMA/HMPC/150801/2015 Committee on Herbal Medicinal Products (HMPC) Assessment report on Salvia officinalis L., folium and Salvia officinalis L., aetheroleum Final Based on Article 16d(1), Article 16f and Article 16h of Directive 2001/83/EC (traditional use) Herbal substance(s) (binomial scientific name of Salvia officinalis L., folium and the plant, including plant part) Salvia officinalis L., aetheroleum Herbal preparation(s) a) Comminuted herbal substance b) Liquid extract (DER 1:1), extraction solvent ethanol 70% V/V c) Dry extract (DER 4-7:1), extraction solvent water d) Liquid extract (DER 1:3.5-5), extraction solvent ethanol 31.5% V/V e) Liquid extract (DER 1:4-5) extraction solvent ethanol 50% V/V f) Liquid extract (DER 1:4-6), extraction solvent liquor wine:ethanol 96% V/V (38.25:61.75 m/m) g) Tincture (ratio of herbal substance to extraction solvent 1:10) extraction solvent ethanol 70% V/V Pharmaceutical form(s) Comminuted herbal substance as herbal tea for oral use. Comminuted herbal substance for infusion preparation for oromucosal or cutaneous use. Herbal preparations in solid or liquid dosage forms for oral use. Herbal preparations in liquid or semi-solid dosage forms for cutaneous use or for oromucosal use. 30 Churchill Place ● Canary Wharf ● London E14 5EU ● United Kingdom Telephone +44 (0)20 3660 6000 Facsimile +44 (0)20 3660 5555 Send a question via our website www.ema.europa.eu/contact An agency of the European Union © European Medicines Agency, 2017. Reproduction is authorised provided the source is acknowledged.
    [Show full text]
  • Determination of Hispidulin in the Flowers of Inula Viscosa (L.) Aiton Using HPLC and HPTLC Methods
    Turk J Pharm Sci 13(2), 159-166, 2016 Original article Determination of Hispidulin in the flowers of Inula viscosa (L.) Aiton Using HPLC and HPTLC Methods Alper GÖKBULUT Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, 06100 Tandoğan, Ankara, TURKEY The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quantified by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatography (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented. Both methods provided a rapid and easy approach for determination of the biomarker hispidulin. In the present study HPTLC and HPLC profiles of Inula viscosa flowers were presented to detect and quantify the small flavonoid hispidulin. The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin. Key words: Hispidulin, Inula viscosa, HPLC, HPTLC Inula viscosa (L.) Aiton Çiçeklerinde YPSK ve YPİTK Yöntemleri ile Hispidulin Analizi Inula viscosa (L.) Aiton çiçeklerinde bir flavon bileşiği olan hispidulin (4´,5,7-trihidroksi-6- metoksiflavon) miktarı Yüksek Performanslı Sıvı Kromatografisi (YPSK) ile tayin edilmiş, ayrıca I. viscosa çiçeklerinin metanolik ekstresinin Yüksek Performanslı İnce Tabaka Kromatografisi (YPİTK) parmakizi kromatogramı da verilmiştir. Her iki yöntem de biyo-işaretleyici bileşen olan hispidulinin belirlenmesi için hızlı ve kolay yaklaşımlar sağlamaktadır. Bu çalışmada küçük bir flavonoit bileşiği olan hispidulinin teşhis ve miktar tayinini gerçekleştirmek amacıyla I. viscosa çiçeklerinin YPİTK ve YPSK profilleri ortaya konmuştur. Sonuçlar, I. viscosa çiçeklerinin önemli ölçüde (0.151±0.007 g/100 g kuru ağırlık) hispidulin içerdiğini göstermektedir. Anahtar kelimeler: Hispidulin, Inula viscosa, YPSK, YPİTK Correspondence: E-mail: [email protected]; Tel: +903122033106 INTRODUCTION known as “yapışkan andız” and its fresh leaves are used for wound healing (25).
    [Show full text]
  • Baicalein Suppresses the Proliferation and Invasiveness of Colorectal Cancer Cells by Inhibiting Snail‑Induced Epithelial‑Mesenchymal Transition
    2544 MOLECULAR MEDICINE REPORTS 21: 2544-2552, 2020 Baicalein suppresses the proliferation and invasiveness of colorectal cancer cells by inhibiting Snail‑induced epithelial‑mesenchymal transition QIONGYAO ZENG1,2, YU ZHANG3,4, WENJING ZHANG2,5 and QIANG GUO1-3 1Faculty of Life Science and Biotechnology; 2Faculty of Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500; 3Department of Gastroenterology, The First People's Hospital of Yunnan Province; 4Yunnan Provincial Institute of Digestive Medicine; 5Department of Medical Oncology, The First Peoples' Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China Received June 7, 2019; Accepted March 6, 2020 DOI: 10.3892/mmr.2020.11051 Abstract. Scutellaria baicalensis (S. baicalensis) is a plant tion (EMT)-promoting factors including vimentin, Twist1, and that is widely used for medicinal purposes. Baicalein, one of Snail, but to upregulate the expression of E‑cadherin in CRC the primary bioactive compounds found in S. baicalensis, is cells. The expression levels of cell cycle inhibitory proteins thought to possess antitumor activity, although the specific p53 and p21 also increased following baicalein treatment. In mechanisms remain unclear. Therefore, the present study addition, Snail-induced vimentin and Twist1 upregulation, as aimed to evaluate the ability of baicalein to disrupt the prolif- well as E‑cadherin downregulation, were reversed following eration and metastatic potential of colorectal cancer (CRC) treatment with baicalein. In conclusion, the results of the cells; a rapid and sensitive ultra-high performance liquid present study indicate that baicalein may suppress EMT, at chromatography-tandem mass spectrometric method was least in part, by decreasing Snail activity. employed for the identification of baicalein in anS.
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2006/0035981 A1 Mazzio Et Al
    US 2006.0035981A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2006/0035981 A1 Mazzio et al. (43) Pub. Date: Feb. 16, 2006 (54) INHIBITION OF ANAEROBIC GLUCOSE Publication Classification METABOLISMAND CORRESPONDING COMPOSITIONAS ANATURAL NON-TOXC (51) Int. Cl. APPROACH TO CANCERTREATMENT A61 K 3L/I 2 (2006.01) A61K 31/7072 (2006.01) A61K 31/7076 (2006.01) A6IK 3I/525 (2006.01) (76) Inventors: Elizabeth Anne Mazzio, Tallahassee, A6IK 36/328 (2006.01) FL (US); Karam F. Soliman, A6IK 36/23 (2006.01) Tallahassee, FL (US) A61K 36/906 (2006.01) (52) U.S. Cl. .............................. 514/690; 514/45; 514/51; Correspondence Address: 514/27; 514/251; 424/725; Karam Soliman 424/748; 424/756; 424/745; Florida A& M University 424/746; 424/729 College of Pharmacy and Pharmaceutical (57) ABSTRACT Sciences This invention discloses a method and formulation for 104. Dyson Building treatment/prevention of human and animal cancers. The Tallahassee, FL 32307 (US) invention is designed to exploit the Vulnerability of cancer with regards to its anaerobic requirement for non-oxidative phosphorylation of glucose to derive energy, which is oppo (21) Appl. No.: 11/233,279 Site to the host. The composition is comprised of a combi nation of one or more of (A) 2,3-dimethoxy-5-methyl-1,4- benzoquinone, ubiquinones (5-45) (B) compound(s) capable (22) Filed: Sep. 20, 2005 of augmenting oxidative phosphorylation Such as a ribofla Vin containing compound and/or ubiquinone (50) (C) 2.3, 4'5,7-pentahydroxyflavone or a lactic acid dehydrogenase Related U.S.
    [Show full text]
  • The Biological Activities and Therapeutic Potentials of Baicalein Extracted from Oroxylum Indicum: a Systematic Review
    molecules Review The Biological Activities and Therapeutic Potentials of Baicalein Extracted from Oroxylum indicum: A Systematic Review Nik Nur Hakimah Nik Salleh , Farah Amna Othman, Nur Alisa Kamarudin and Suat Cheng Tan * School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia; [email protected] (N.N.H.N.S.); [email protected] (F.A.O.); [email protected] (N.A.K.) * Correspondence: [email protected]; Tel.: +60-9-7677776 Academic Editors: Giuseppe Caruso, Nicolò Musso and Claudia Giuseppina Fresta Received: 1 November 2020; Accepted: 23 November 2020; Published: 2 December 2020 Abstract: In Southeast Asia, traditional medicine has a longestablished history and plays an important role in the health care system. Various traditional medicinal plants have been used to treat diseases since ancient times and much of this traditional knowledge remains preserved today. Oroxylum indicum (beko plant) is one of the medicinal herb plants that is widely distributed throughout Asia. It is a versatile plant and almost every part of the plant is reported to possess a wide range of pharmacological activities. Many of the important bioactivities of this medicinal plant is related to the most abundant bioactive constituent found in this plant—the baicalein. Nonetheless, there is still no systematic review to report and vindicate the biological activities and therapeutic potential of baicalein extracted from O. indicum to treat human diseases. In this review, we aimed to systematically present in vivo and in vitro studies searched from PubMed, ScienceDirect, Scopus and Google Scholar database up to 31 March 2020 based on keywords “Oroxylum indicum” and “baicalein”.
    [Show full text]
  • Evidence for Aminopeptidase-N (CD13) Inhibition, Antiproliferative and Cell Death Properties
    AIMS Molecular Science, 3(3): 368-385. DOI: 10.3934/molsci.2016.3.368 Received 17 May 2016, Accepted 28 July 2016, Published 1 August 2016 http://www.aimspress.com/journal/Molecular Research article In vitro activity of some flavonoid derivatives on human leukemic myeloid cells: evidence for aminopeptidase-N (CD13) inhibition, antiproliferative and cell death properties Sandrine Bouchet1,2, Marion Piedfer3, Santos Susin1, Daniel Dauzonne4,#, and Brigitte Bauvois1,#,* 1 Centre de Recherche des Cordeliers, INSERM UMRS1138, Sorbonne Universités UPMC Paris 06, Université Paris Descartes Sorbonne Paris Cité, F-75006 Paris, France 2 Assistance Publique des Hôpitaux de Paris, France 3 Centre de Recherche des Cordeliers, INSERM UMRS872 (2011-2012), Sorbonne Universités UPMC Paris 06, Université Paris Descartes Sorbonne Paris Cité, F-75006 Paris, France 4 Institut Curie, Departement Recherche, CNRS UMR3666, INSERM U1143, F-75005 Paris, France # Senior co-authorship. * Correspondence: Email: [email protected]; Tel: +33-144-273-138; Fax: +33-144-273-131. Abstract: Leukemia cells from patients with acute myeloid leukemia (AML) display high proliferative capacity and are resistant to death. Membrane-anchored aminopeptidase-N/CD13 is a potential drug target in AML. Clinical research efforts are currently focusing on targeted therapies that induce death in AML cells. We previously developed a non-cytotoxic APN/CD13 inhibitor based on flavone-8-acetic acid scaffold, the 2',3-dinitroflavone-8-acetic acid (1). In this context, among the variously substituted 113 compounds further synthesized and tested for evaluation of their effects on APN/CD13 activity, proliferation and survival in human AML U937 cells, eight flavonoid derivatives emerged: 2',3-dinitro-6-methoxy-flavone-8-acetic acid (2), four compounds (3–6) with the 3-chloro-2,3-dihydro-3-nitro-2-phenyl-4H-1-benzopyran-4-one structure, and three (7–9) with the 3-chloro-3,4-dihydro-4-hydroxy-3-nitro-2-phenyl-2H-1-benzopyran framework.
    [Show full text]
  • Isorhamnetin and Hispidulin from Tamarix Ramosissima Inhibit
    foods Article Isorhamnetin and Hispidulin from Tamarix ramosissima Inhibit 2-Amino-1-Methyl-6- Phenylimidazo[4,5-b]Pyridine (PhIP) Formation by Trapping Phenylacetaldehyde as a Key Mechanism Xiaopu Ren 1,2 , Wei Wang 1, Yingjie Bao 1, Yuxia Zhu 1, Yawei Zhang 1, Yaping Lu 3, Zengqi Peng 1,* and Guanghong Zhou 1 1 Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; [email protected] (X.R.); [email protected] (W.W.); [email protected] (Y.B.); [email protected] (Y.Z.); [email protected] (Y.Z.); [email protected] (G.Z.) 2 Xinjiang Production & Construction Group Key Laboratory of Agricultural Products Processing in Xinjiang South, College of Life Science, Tarim University, Alar 843300, China 3 College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; [email protected] * Correspondence: [email protected]; Tel.: +86-25-84396558 Received: 7 March 2020; Accepted: 18 March 2020; Published: 3 April 2020 Abstract: Tamarix has been widely used as barbecue skewers to obtain a good taste and a unique flavor of roast lamb in China. Many flavonoids have been identified from Tamarix, which is an important strategy employed to reduce the formation of heterocyclic amines (HAs) in roast meat. Isorhamnetin, hispidulin, and cirsimaritin from Tamarix ramosissima bark extract (TRE) effectively inhibit the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP), the most abundant HAs in foods, both in roast lamb patties and in chemical models.
    [Show full text]
  • Or Blood Endothelial Disintegration Induced by Colon Cancer Spheroids SW620
    molecules Article Flavonoids Distinctly Stabilize Lymph Endothelial- or Blood Endothelial Disintegration Induced by Colon Cancer Spheroids SW620 1,2, 1,2, 1,2, 1 2 Julia Berenda y, Claudia Smöch y, Christa Stadlbauer y, Eva Mittermair , Karin Taxauer , Nicole Huttary 2, Georg Krupitza 2 and Liselotte Krenn 1,* 1 Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, A-1090 Vienna, Austria; [email protected] (J.B.); [email protected] (C.S.); [email protected] (C.S.); [email protected] (E.M.) 2 Department of Pathology, Medical University of Vienna, A-1090 Vienna, Austria; [email protected] (K.T.); [email protected] (N.H.); [email protected] (G.K.) * Correspondence: [email protected] These authors contributed equally to this work. y Received: 16 April 2020; Accepted: 28 April 2020; Published: 29 April 2020 Abstract: The health effects of plant phenolics in vegetables and other food and the increasing evidence of the preventive potential of flavonoids in “Western Diseases” such as cancer, neurodegenerative diseases and others, have gained enormous interest. This prompted us to investigate the effects of 20 different flavonoids of the groups of flavones, flavonols and flavanones in 3D in vitro systems to determine their ability to inhibit the formation of circular chemorepellent induced defects (CCIDs) in monolayers of lymph- or blood-endothelial cells (LECs, BECs; respectively) by 12(S)-HETE, which is secreted by SW620 colon cancer spheroids. Several compounds reduced the spheroid-induced defects of the endothelial barriers. In the SW620/LEC model, apigenin and luteolin were most active and acacetin, nepetin, wogonin, pinocembrin, chrysin and hispidulin showed weak effects.
    [Show full text]
  • 67 1. ABSTRACT 2. INTRODUCTION Phytochemicals in the Treatment Of
    [Frontiers in Bioscience, Elite, 9, 67-75, January 1, 2017] Phytochemicals in the treatment of ovarian cancer Juhi Rais1, Asif Jafri1, Sahabjada Siddiqui1, Madhu Tripathi1, Mohammad Arshad1 1Department of Zoology, University of Lucknow, Lucknow, India 226007 TABLE OF CONTENTS 1. Abstract 2. Introduction 3. Classification of phytochemicals 3.1. Terpenes 3.1.1. Carotenoids 3.1.2. Monterpenoids 3.1.2.1. Perillyl alcohol 3.1.2.2. Limonene 3.1.3. Saponins 3.2. Phenols 3.2.1. Polyphenols 3.2.2. Flavonoids 3.3. Organo-sulphur compounds 3.3.1. Indoles and isothiocyanates 3.3.2. Thiosulfonates 3.4. Organic acid and polysaccharides 3.4.1. Organic acids 3.4.2. Polysaccharides 3.5. Lipids 3.5.1. Isoprenoids 3.5.2. Omega-3 and omega-6 fatty acid 4. Mode of action of phytochemicals against ovarian cancer 5. References 1. ABSTRACT Ovarian cancer ranks 5th among the most which includes serous, mucinous, clear cell, squamous common gynecologic cancers and causes the highest and endometrioid carcinoma (3, 4). The distinct mortality in females. Here, we discuss the role of a group molecular and genetic profiles of these cancer types of natural products that are being used in treatment and and their varied response to treatment makes it difficult prevention of a host of cancers including ovarian cancer. to design a common therapeutic regimen (3). The tumor Some plants and nutraceuticals and their polyphenolic microenvironment of ovarian carcinoma is comprised of constituents such as flavones, flavonoids, and antioxidants fibroblasts, immune cells, and endothelial cells of the have shown cytotoxic effects on cancer cells both in vitro host as well as non-cellular components including the and in vivo.
    [Show full text]
  • Medicinal Importance, Pharmacological Activities, and Analytical Aspects of Hispidulin: a Concise Report
    Journal of Traditional and Complementary Medicine xxx (2016) Contents lists available at ScienceDirect Journal of Traditional and Complementary Medicine journal homepage: http://www.elsevier.com/locate/jtcme Review article Medicinal importance, pharmacological activities, and analytical aspects of hispidulin: A concise report * Kanika Patel a, Dinesh Kumar Patel b, a Jubilant Life Sciences, Noida, Uttar Pradesh, 201301, India b Department of Pharmacy, Galgotias University, Greater Noida, 203201, Uttar Pradesh, India article info abstract Article history: Herbal medicines have been played an important role in the human civilization since very ancient time Received 11 April 2016 as a food, cloth, medicine and other aspects. Some of the important drugs in the modern medicine were Received in revised form derived from the natural sources such as aspirin, digitalis, quinine, vincristine, vinblastine etc. Hispidulin 3 November 2016 (40, 5, 7-trihydroxy-6-methoxyflavone) is a flavones derivative found in plant such as Grindelia argentina, Accepted 22 November 2016 Arrabidaea chica, Saussurea involucrate, Crossostephium chinense, Artemisia and Salvia species. Hispidulin Available online xxx have antioxidant, antifungal, anti-inflammatory, antimutagenic, and antineoplastic properties. So far, various analytical methods have been investigated and developed for detection of hispidulin in the plant Keywords: Analytical aspects materials. Productions of hispidulin through different tissue culture techniques have been also investi- Herbal medicine gated. Present review summarized medicinal uses, pharmacological activities and analytical aspects of Hispidulin hispidulin. From the above mentioned aspects, we can conclude that, this review will be helpful to the Medicinal aspects researcher in the field of natural product for the development of novel molecule for the treatment of Pharmacological activities different disorders.
    [Show full text]
  • Enhancement of the Antiobesity and Antioxidant Effect of Purple Sweet Potato Extracts and Enhancement of the Effects by Fermentation
    antioxidants Article Enhancement of the Antiobesity and Antioxidant Effect of Purple Sweet Potato Extracts and Enhancement of the Effects by Fermentation Seul Gi Lee 1,2,3 , Jongbeom Chae 3, Dong Se Kim 3, Jung-Bok Lee 4,5 , Gi-Seok Kwon 5, Taeg Kyu Kwon 1,2 and Ju-Ock Nam 3,6,* 1 Department of Immunology, School of Medicine, Keimyung University, Daegu 42601, Korea; [email protected] (S.G.L.); [email protected] (T.K.K.) 2 Center for Forensic Pharmaceutical Science, Keimyung University, Daegu 42601, Korea 3 Department of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea; [email protected] (J.C.); [email protected] (D.S.K.) 4 Kyochon Research & Innovation Center, Kyochon F&B Co., Ltd., Chilgok-gun 18469, Korea; [email protected] 5 Department of Medical Plant Resources, Andong National University, Andong 36729, Korea; [email protected] 6 Institute of Agricultural Science & Technology, Kyungpook National University, Daegu 41566, Korea * Correspondence: [email protected]; Tel.: +82-53-950-7760; Fax: +82-53-950-7762 Abstract: The browning of white adipocytes, which transforms energy-storing white adipocytes to Citation: Lee, S.G.; Chae, J.; Kim, heat-producing beige adipocytes, is considered a strategy against metabolic diseases. Several dietary D.S.; Lee, J.-B.; Kwon, G.-S.; Kwon, compounds, such as anthocyanins, flavonoids, and phenolic acids, induce a brown adipocyte-like T.K.; Nam, J.-O. Enhancement of the phenotype in white adipocytes. In this study, we demonstrated that purple sweet potato (Ipomoea Antiobesity and Antioxidant Effect of batatas) extract (PSP) exhibited potent radical scavenging activity.
    [Show full text]
  • Schisandrin B for Treatment of Male Infertility
    bioRxiv preprint doi: https://doi.org/10.1101/2020.01.20.912147; this version posted January 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Schisandrin B for treatment of male infertility Di-Xin Zou†1,2,3, Xue-Dan Meng†1,2,3, Ying Xie1, Rui Liu1, Jia-Lun Duan1, Chun-Jie Bao1, Yi-Xuan Liu1, Ya-Fei Du1, Jia-Rui Xu1, Qian Luo1, Zhan Zhang1, Shuang Ma1, Wei-Peng Yang*3, Rui-Chao Lin*2, Wan-Liang Lu*1 1. State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, and School of Pharmaceutical Sciences, Peking University, Beijing 100191, China 2. Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China 3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China † Both authors contributed equally to this work. Correspondence to: [email protected] Dr. Wan-Liang Lu, Ph.D. Professor School of Pharmaceutical Sciences Peking University No 38, Xueyuan Rd, Beijing 100191, China Tel & Fax: +8610 82802683 * Corresponding authors Dr. Wan-Liang Lu, [email protected] Dr. Rui-Chao Lin, [email protected] Dr. Wei-Peng Yang, [email protected] Abstract The decline of male fertility and its consequences on human populations are important public-health issues. However, there are limited choices for treatment of male infertility. In an attempt to identify a compound that could promote male fertility, we identified and characterized a library of small molecules from an ancient formulation Wuzi Yanzong-Pill, which was used as a folk medicine since the Tang dynasty of China.
    [Show full text]