DOCSLIB.ORG

Expanding the Portfolio of Synthetic Biology Tools in Saccharomyces

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Expanding the Portfolio of Synthetic Biology Tools in Saccharomyces Ghent University Faculty of Bioscience Engineering Department of Biotechnology Expanding the portfolio of synthetic biology tools in Saccharomyces cerevisiae for the optimization of heterologous production pathways at the transcriptional and translational level Thomas Decoene Thesis submitted in fulfillment of the requirements for the degree of Doctor (Ph.D.) in Applied Biological Sciences Academic year 2017-2018 Examination committee Prof. John Van Camp (Ghent University) (chairman) Prof. Yves Briers (Ghent University) (secretary) Prof. Els Van Damme (Ghent University) Prof. Alain Goossens (Ghent University, VIB) Prof. Ronnie Willaert (Free University of Brussels) Supervisors Prof. Marjan De Mey (Ghent University) dr. Sofie De Maeseneire (Ghent University) Dean Prof. Marc Van Meirvenne Rector Prof. Rik Van de Walle ii Ghent University Faculty of Bioscience Engineering Department of Biotechnology Expanding the portfolio of synthetic biology tools in Saccharomyces cerevisiae for the optimization of heterologous production pathways at the transcriptional and translational level Thomas Decoene Thesis submitted in fulfillment of the requirements for the degree of Doctor (Ph.D.) in Applied Biological Sciences Academic year 2017-2018 iii Dutch translation of the title: Uitbreiding van het portfolio aan synthetische biologie tools in Saccharomyces cerevisiae voor de optimalisatie van heterologe productie pathways op het transcriptionele en translationele niveau To refer to this thesis: Decoene, T., 2018. Expanding the portfolio of synthetic biology tools in Saccharomyces cerevisiae for the optimization of heterologous production pathways at the transcriptional and translational level. Ph.D. thesis, Ghent University. Cover illustration: vska (123RF) ISBN number: 9789463570961 Copyright ©2018 by Thomas Decoene. The author and the promoters give the authorization to consult and copy parts of this work for personal use only. Every other use is subject to the copyright laws. Permission to reproduce any material contained in this work should be obtained from the author. Thomas Decoene was supported by a fellowship of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen). iv WOORD VOORAF Dit is het dan, het schrijven van de laatste bladzijden van mijn ‘boekske’ . Die vier jaar onderzoek neerpennen achtte ik vorig jaar nog onmogelijk en was hetgeen waar ik zwaar tegen opzag. Uiteindelijk ben ik wel heel tevreden dat mijn doctoraat hier eindelijk ligt. Werken aan een doctoraat was een zalige periode, waar ik de volledige vrijheid had om me bij te scholen op tal van vlakken. Echter was het ook vaak een lastige tijd, waar tegenslagen in experimenten en de zoveelste ‘scoop’ ervoor zorgden dat ik me dikwijls afvroeg waar ik in godsnaam aan begonnen was. Dit doctoraat zou er dan ook nooit gelegen hebben zonder de aanwezigheid en hulp van een fantastische groep mensen, zowel op als buiten het labo, die ervoor gezorgd hebben dat ik de zin had om te blijven doorzetten. Bij deze wil ik hen hier dus heel graag bedanken! Om te beginnen aan een doctoraat heb je natuurlijk iemand nodig die in je gelooft en je de komende vier jaar ten volle wil steunen, een mentor of een promoter zoals dat dan heet in officiële termen. Marjan, daarvoor wil ik jou in eerste plaats bedanken! Je hebt me de kans gegeven om met bakkersgist, toen ook voor jou nog een vrij onbekend organisme, te beginnen binnen de MEMO groep. Ik kreeg de volledige ruimte om nieuwe technieken binnen het veld van de synthetische biologie in gist uit te proberen en mijn kennis bij te schaven op nationale en internationale congressen. Voor dit vertrouwen ben ik je oprecht zeer dankbaar! Uiteraard ook een welgemeende dankjewel aan mijn co-promoter Sofie, de bakkersgist post-doc op het labo! Altijd kon ik bij je terecht met allerhande vragen en het grondig nalezen van mijn teksten apprecieer ik enorm! Daarnaast zorgde je vaak voor de amusante momenten in de MEMO bureau; zo was het altijd hilarisch wanneer het fameuze ‘mannekes’ weer maar eens door de bureau galmde toen het boeltje er op stelten stond. Dit brengt me dan ook naadloos bij het MEMO hoofdstuk. Als thesisstudent kwam ik terecht in een gedreven bende enthousiastelingen waar er naast wetenschappelijk onderzoek plaats was voor zeer veel ambiance. Deze goede sfeer heeft er immers grotendeels toe bijgedragen dat ik overtuigd was om hier te starten met een doctoraat. Mijn voorgangers waren het drietal Frederik, Gert en Pieter. Al tijdens het schrijven van ons IWT voorstel werden we ingewijd in de groep met de ‘Russische avond’ georganiseerd door Frederik, een v legendarische avond die nog bij velen van ons in het geheugen gegrift staat. Ook Gert en Pieter waren er van in het begin bij en wil ik bedanken voor de input omtrent experimentele set-ups en data-analyse. Pieter bruiste van de zotste ideeën en wou als het enigszins mogelijk was zijn labowerk laten uitvoeren door robots. Gert lag mee aan de basis van het yUTR verhaal, [na een zoveelste scoop ] ‘Thomas, kga helpen uw doctoraat redden’, of hoe bijgevolg een varia projectje is uitgedraaid op een mooi hoofdstuk en artikel, bedankt daarvoor! Op dat moment ook nog aanwezig waren Joeri, die als het ware volledige metabolische pathways uit zijn hoofd kende, en Gaspard, anti-Windows en me dus altijd maar vragen als ik geen Ubuntu cd’tje moest hebben. Samen met Bob en Brecht vormde ik dan de volgende lichting. Bob, veelal bezig met de laatste ‘geek stuff’ (3D printers, zichzelf laten chippen, etc. ) en zeer belezen omtrent de laatste synthetische biologie trends. Brecht, kortweg Paepe had altijd wel een ludiek verhaal in petto; ‘De Groote Gaston ’ en het scheidingsmisverstand van ‘de Xavier’ zijn maar een paar voorbeelden uit het brede oeuvre. Als ik de voorbije vier jaar ook ooit één serieus woord met je gewisseld heb zal het veel geweest zijn. Uitspraken zoals ‘ Ja, mijn grijze plek is ook een biosensor’ waren van die hilarische momenten. Tevens denk ik nog vaak met een grote glimlach terug aan onze trip met Nico vorig jaar naar Singapore en Indonesië. Btw, laat me zeker weten wanneer Biosensor Centre Paepe uit de startblokken schiet! Intussen werd ook onze opvolging verzekerd. Tom, de stille harde werker. Samen hebben we toch enkele mooie systemen uitgewerkt in het labo om onze geliefde gastheer bakkersgist te temmen. Maarten VB, je mag dan wel de ‘man van glas’ genoemd worden, toch wist je er in te slagen om een veel winnaar te zijn, getuige daarvan je overwinning op het schuttersparcours aan Dikkebusvijver, de topscore bij de bureaudarts en je recente NAR publicatie. David, altijd bereid om mijn West-Vlaamse zinsconstructies te vertalen en te ontleden, en me bijgevolg op linguïstisch vlak wat bij te scholen. Mol, het officieuze MEMO lid, labo feestpreases en ‘drager der korte broeken’ . Verbazend hoe je er telkens in slaagde om alle laatste labogeroddel te weten te komen. Lien en Chiara, die de mannelijke hegemonie in de groep durfden te doorbreken. Bedankt ook voor de organisatie van het fantastische MEMO weekend in Parijs! De groep zou uiteraard niet hetzelfde zijn zonder Jo. Ook jij bent er al van bij het schrijven van onze projecten bij en zorgde ervoor dat we ons bleven focussen om die wetenschappelijke doelen te halen. Daarnaast was je altijd een amusante metgezel om wat subtiele stekjes te geven aan Sofie, wat dan meestal uitdraaide in een leuk verbaal post-doc gevechtje. Ondanks de zware jaren blijf ik ook jouw immense kracht en moed om te blijven vi gaan bewonderen! Last but not least mogen we uiteraard het tweetal Wouter en Dries niet vergeten. Mister W, de grootmeester van het praktisch labowerk en de Koepuur jukebox. Al vanaf mijn thesis kon ik je lastig vallen met allerlei vragen omtrent moleculair en analytisch werk en trapte je Koepuur avonden op gang alsof het niets was. El Duchi, meermaals slaagde je erin om me het bloed van onder de nagels te halen door weer maar eens af te geven op de triestige gisten die toch niets konden of me uit te maken voor successupportertje. Samen met Wouter was je ook gepassioneerd door voetbal en sport, wat het altijd leuk maakte om onze visies te laten schijnen over dat prachtige doelpunt of die bijzondere koers. Ook wil ik graag alle Glycodirect, BioPort en InBio collega’s bedanken. Eén voor één fantastische mensen die allemaal bijdragen tot een formidabele sfeer op het labo wat het werken des te aangenamer maakt. Bewijs daarvan de vele post labo-activiteiten zoals de kerstfeestjes, het paasontbijt, de barbecues, labo-uitstappen, een pint pakken in de Koe en de verscheidene sportactiviteiten. Zeker op sportvlak heb ik me op het labo altijd ten volle kunnen uitleven! Dankjewel daarvoor aan Margo, Stevie, Jorick, Koen, Sylwia, Mol, Maarten VB en recentelijk Veerle, Sven, Jelle en Matthieu. Samen vormden we het Inbiyolo/CS Barcelona minivoetbalteam, een amusante ploeg waar het motto ‘deelnemen is belangrijker dan winnen’ echt van tel was. Vaste afsluiter was dan ook het doorspoelen van het verlies of het vieren van een zoet smakende (zeldzame) overwinning achteraf in het GUSB, altijd een leuk moment! Daarnaast vertegenwoordigden we het labo jaarlijks op de Mister T triatlon met een bende topatleten. Merci daarvoor aan Dries D, Tom V, Martijn, Griet, Magali, Margo, Sophie, Karel, Maarten D, Gert, Hannes en Stevie om er telkens een geslaagde namiddag van te maken. Ook werden er op zonnige lente – en zomeravonden met het labofietsteam menige kilometers afgemaald. Nooit zal ik onze legendarische fietstocht met Gert, Robin, Maarten D, Magali, Karel en Stevie naar Brugge vergeten! En naar het schijnt zouden we nog eens zoiets over doen richting Roeselare… Een speciaal woordje van dank gaat ook uit naar de Yeastpalace . Vroeger nog met Lien en Isabelle, en vandaag de dag met het jongere geweld Tom, Mol, Veerle en Yatti, een zeer aangename plek waar er tussen het werken door veel gelachen werd.
Load more

Recommended publications
  • The Cinnamic Acid Pathway and Hispidin Biosynthesis In
    THE CINNAMIC ACID PATHWAY AND HISPIDIN BIOSYNTHESIS IN CULTURES OF POLYPORUS HISPIDUS FRIES by PETER WILLIAM PERRIN B.Sc, University of British Columbia, 1968 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in the Department of Botany We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA September, 1972 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying'of this thesis for scholarly purposes may be granted by the Head of my Department or by his representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of The University of British Columbia Vancouver 8, Canada Date i ABSTRACT The biosynthesis of hispidin, 6-(3,4-dihydroxystyryl)- 4-hydroxy-2-pyrone, was examined in cultures of Polyporus hispidus Fr. Cultural studies were undertaken to determine the most suitable medium for investigating the biosynthesis of this pigment. These studies showed that light was nec• essary for hispidin formation and that the development of basidiocarps with viable spores could be achieved on agar media. On the liquid medium employed for biochemical studies, the maximum rate of hispidin production was observed to lag the maximum rate of growth by about five days. Trimethylhispidin, 4-methylhispidin and yangonin were synthesized for comparative purposes and for dilution in tracer experiments.
    [Show full text]
  • Effects of Enzymatic and Thermal Processing on Flavones, the Effects of Flavones on Inflammatory Mediators in Vitro, and the Absorption of Flavones in Vivo
    Effects of enzymatic and thermal processing on flavones, the effects of flavones on inflammatory mediators in vitro, and the absorption of flavones in vivo DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Gregory Louis Hostetler Graduate Program in Food Science and Technology The Ohio State University 2011 Dissertation Committee: Steven Schwartz, Advisor Andrea Doseff Erich Grotewold Sheryl Barringer Copyrighted by Gregory Louis Hostetler 2011 Abstract Flavones are abundant in parsley and celery and possess unique anti-inflammatory properties in vitro and in animal models. However, their bioavailability and bioactivity depend in part on the conjugation of sugars and other functional groups to the flavone core. Two studies were conducted to determine the effects of processing on stability and profiles of flavones in celery and parsley, and a third explored the effects of deglycosylation on the anti-inflammatory activity of flavones in vitro and their absorption in vivo. In the first processing study, celery leaves were combined with β-glucosidase-rich food ingredients (almond, flax seed, or chickpea flour) to determine test for enzymatic hydrolysis of flavone apiosylglucosides. Although all of the enzyme-rich ingredients could convert apigenin glucoside to aglycone, none had an effect on apigenin apiosylglucoside. Thermal stability of flavones from celery was also tested by isolating them and heating at 100 °C for up to 5 hours in pH 3, 5, or 7 buffer. Apigenin glucoside was most stable of the flavones tested, with minimal degradation regardless of pH or heating time.
    [Show full text]
  • Recent Developments in Identification of Genuine Odor- and Taste-Active Compounds in Foods
    Recent Developments in Identification of Genuine Odor- and Taste-Active Compounds in Foods Edited by Remedios Castro-Mejías and Enrique Durán-Guerrero Printed Edition of the Special Issue Published in Foods www.mdpi.com/journal/foods Recent Developments in Identification of Genuine Odor- and Taste-Active Compounds in Foods Recent Developments in Identification of Genuine Odor- and Taste-Active Compounds in Foods Editors Remedios Castro-Mej´ıas Enrique Dur´an-Guerrero MDPI Basel Beijing Wuhan Barcelona Belgrade Manchester Tokyo Cluj Tianjin • • • • • • • • • Editors Remedios Castro-Mej´ıas Enrique Duran-Guerrero´ Analytical Chemistry Analytical Chemistry Universidad de Cadiz´ Department Puerto Real University of Cadiz Spain Puerto Real Spain Editorial Office MDPI St. Alban-Anlage 66 4052 Basel, Switzerland This is a reprint of articles from the Special Issue published online in the open access journal Foods (ISSN 2304-8158) (available at: www.mdpi.com/journal/foods/special issues/Recent Developments Identification Genuine Odor- Taste-Active Compounds Foods). For citation purposes, cite each article independently as indicated on the article page online and as indicated below: LastName, A.A.; LastName, B.B.; LastName, C.C. Article Title. Journal Name Year, Volume Number, Page Range. ISBN 978-3-0365-1668-4 (Hbk) ISBN 978-3-0365-1667-7 (PDF) © 2021 by the authors. Articles in this book are Open Access and distributed under the Creative Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons license CC BY-NC-ND.
    [Show full text]
  • Biochemical Investigations in the Rare Disease Alkaptonuria: Studies on the Metabolome and the Nature of Ochronotic Pigment
    Biochemical Investigations in the Rare Disease Alkaptonuria: Studies on the Metabolome and the Nature of Ochronotic Pigment Thesis submitted in accordance with the requirements of the University of Liverpool for the degree of Doctor of Philosophy by Brendan Paul Norman September 2019 ACKNOWLEDGEMENTS It is hard to describe the journey this PhD has taken me on without reverting to well-worn clichés. There has been plenty of challenges along the way, but ultimately I can look back on the past four years with a great sense of pride, both in the work presented here and the skills I have developed. Equally important though are the relationships I have established. I have lots of people to thank for playing a part in this thesis. First, I would like to thank my supervisors, Jim Gallagher, Lakshminarayan Ranganath and Norman Roberts for giving me this fantastic opportunity. Your dedication to research into alkaptonuria (AKU) is inspiring and our discussions together have always been thoughtful and often offered fresh perspective on my work. It has been a pleasure to work under your supervision and your ongoing support and encouragement continues to drive me on. It has truly been a pleasure to be part of the AKU research group. Andrew Davison deserves a special mention - much of the highs and lows of our PhD projects have been experienced together. Learning LC-QTOF-MS was exciting (and continues to be) but equally daunting at the start of our projects (admittedly more so for me as a Psychology graduate turned mass spectrometrist!). I am very proud of what we have achieved together, largely starting from scratch on the instrument, and we are continuing to learn all the time.
    [Show full text]
  • Discovery of the Potential Biomarkers for Discrimination Between Hedyotis Diffusa and Hedyotis Corymbosa by UPLC-QTOF/MS Metabolome Analysis
    molecules Article Discovery of the Potential Biomarkers for Discrimination between Hedyotis diffusa and Hedyotis corymbosa by UPLC-QTOF/MS Metabolome Analysis Yaru Wang 1, Cuizhu Wang 1, Hongqiang Lin 1, Yunhe Liu 1, Yameng Li 1, Yan Zhao 2, Pingya Li 1 and Jinping Liu 1,* ID 1 School of Pharmaceutical Sciences, Jilin University, Fujin Road 1266, Changchun 130021, China; [email protected] (Y.W.); [email protected] (C.W.); [email protected] (H.L.); [email protected] (Yu.L.); [email protected] (Ya.L.); [email protected] (P.L.) 2 College of Chinese Medicinal Materials, Jilin Agriculture University, Xincheng Street 2888, Changchun 130118, China; [email protected] * Correspondence: [email protected]; Tel.: +86-431-8561-9803 Received: 11 May 2018; Accepted: 22 June 2018; Published: 25 June 2018 Abstract: Hedyotis diffuse Willd. (HD) and Hedyotis corymbosa (L.) Lam. (HC), two closely related species of the same genus, are both used for health benefits and disease prevention in China. HC is also indiscriminately sold as HD in the wholesale chain and food markets. This confusion has led to a growing concern about their identification and quality evaluation. In order to further understand the molecular diversification between them, we focus on the screening of chemical components and the analysis of non-targeted metabolites. In this study, UPLC-QTOF-MSE, UNIFI platform and multivariate statistical analyses were used to profile them. Firstly, a total of 113 compounds, including 80 shared chemical constituents of the two plants, were identified from HC and HD by using the UNIFI platform.
    [Show full text]
  • Yeast Cell Factory-Platform for the Screening and the Industrial
    Roskilde University Yeast Cell Factory-Platform for the Screening and the Industrial Production of Flavonoids and other Phenolic Compounds PhD thesis by Beata Joanna Lehka Lehka, Beata Joanna Publication date: 2017 Document Version Publisher's PDF, also known as Version of record Citation for published version (APA): Lehka, B. J. (2017). Yeast Cell Factory-Platform for the Screening and the Industrial Production of Flavonoids and other Phenolic Compounds: PhD thesis by Beata Joanna Lehka. Roskilde Universitet. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain. • You may freely distribute the URL identifying the publication in the public portal. Take down policy If you believe that this document breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 09. Oct. 2021 Yeast Cell Factory-Platform for the Screening and the Industrial Production of Flavonoids and other Phenolic Compounds PhD THESIS BY BEATA JOANNA LEHKA MAY 2017 Supervisors: Ernesto Simon and Assoc. Prof. Håvard Jenssen Submission date: 31/05/2017 The thesis has been submitted to the Department of Science and Environment, Roskilde University, Denmark i PREFACE AND ACKNOWLEDGEMENTS This thesis presents work done during my PhD study.
    [Show full text]
  • The Biosynthetic Origin of Psychoactive Kavalactones in Kava
    The biosynthetic origin of psychoactive kavalactones in kava 1 1 1,2 1,2 Tomáš Pluskal ,​ Michael P. Torrens-Spence ,​ Timothy R. Fallon ,​ Andrea De Abreu ,​ ​ ​ ​ ​ 1,2 1,2,* Cindy H. Shi ,​ and Jing-Ke Weng ​ ​ 1 Whitehead​ Institute for Biomedical Research, 455 Main Street, Cambridge, MA 02142 USA. 2 Department​ of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 USA. * Corresponding​ author. Email: [email protected] ​ Kava (Piper methysticum) is an ethnomedicinal shrub native to the Polynesian islands with ​ ​ well-established anxiolytic and analgesic properties. Its main psychoactive principles, kavalactones, form a unique class of polyketides that interact with the human central nervous system through mechanisms distinct from those of conventional psychiatric drugs. However, an unknown biosynthetic machinery and difficulty in chemical synthesis hinder the therapeutic use of kavalactones. In addition, kava contains several chalconoids with reported anti-cancer properties, known as flavokavains. Here, we report de novo ​ elucidation of key components of the kavalactone and flavokavain biosynthetic network, encompassing eight specialized metabolic enzymes. We present the structural basis for the evolutionary development of a pair of paralogous styropyrone synthases that establish the kavalactone scaffold as well as the catalytic mechanism of a regio- and stereo-specific kavalactone reductase that produces a subset of chiral kavalactones. We further demonstrate the feasibility of engineering kavalactone production
    [Show full text]
  • Increased Phenolic Content in Apple Leaves Infected with the Apple Scab Pathogen
    007_TESTO_667_049 22-02-2008 17:58 Pagina 49 Journal of Plant Pathology (2008), 90 (1), 49-55 Edizioni ETS Pisa, 2008 49 INCREASED PHENOLIC CONTENT IN APPLE LEAVES INFECTED WITH THE APPLE SCAB PATHOGEN M. Mikulic Petkovsˇek, F. Stampar and R. Veberic Department of Agronomy, Chair of Fruit Growing, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia SUMMARY chemicals belong to the phenolic group (Grayer and Kokubun, 2001), which are involved in the natural de- Phenolic compounds were measured in leaf tissues of fense reactions of apples against various diseases, being apple cvs Jonagold and Golden Delicious, healthy and toxic to pathogens. These compounds are produced infected by Venturia inaequalis. Leaves were sampled and accumulate at a faster rate after infection (Picinelli from May to September 2005 and analyzed by high per- et al., 1995; Usenik et al., 2004; Treutter, 2005). formance liquid chromatography. Hydroxycinnamic Phenolics, flavanols in particular, play a role in the acids detected were chlorogenic, caffeic, ferulic and p- resistance of apples to V. inaequalis (Treutter and coumaric. In addition, the presence was ascertained of Feucht, 1990a, 1990b). The ability to accumulate fla- the dihydrochalcone phloridzin and the flavonoids epi- vanols after infection in the tissue surrounding infected catechin, catechin, rutin and quercitrin. Total phenolics sites is a differential property of susceptible and resist- were determined with the Folin-Ciocalteu method. In- ant cultivars (Treutter and Feucht, 1990b), and consti- fection by V. inequalis caused an accumulation of phe- tutes additional evidence that these compounds are in- nolic compounds in infected leaves with a 1.4 to 6.2- volved in the defense mechanism against apple scab fold increase of flavonols, a 2 to 6-fold increase of (Mayr et al., 1995).
    [Show full text]
  • Improving Heterologous Production of Phenylpropanoids in Saccharomyces Cerevisiae by Tackling an Unwanted Side Reaction of Tsc13, an Endogenous Double Bond Reductase
    Roskilde University Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double bond reductase Lehka, Beata Joanna; Eichenberger, Michael; Yoshimoto, Walden Emil Bjørn; Garcia Vanegas, Katherina; Buijs, Nicolaas ; Jensen, Niels Bjerg ; Dannow Dyekjær, Jane; Jenssen, Håvard; Simon, Ernesto; Naesby, Michael Published in: FEMS Yeast Research DOI: 10.1093/femsyr/fox004 Publication date: 2017 Document Version Publisher's PDF, also known as Version of record Citation for published version (APA): Lehka, B. J., Eichenberger, M., Yoshimoto, W. E. B., Garcia Vanegas, K., Buijs, N., Jensen, N. B., Dannow Dyekjær, J., Jenssen, H., Simon, E., & Naesby, M. (2017). Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double bond reductase. FEMS Yeast Research, 17(1). https://doi.org/10.1093/femsyr/fox004 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain. • You may freely distribute the URL identifying the publication in the public portal. Take down policy If you believe that this document breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim.
    [Show full text]
  • The Reciprocal Interactions Between Polyphenols and Gut Microbiota and Effects on Bioaccessibility
    nutrients Review The Reciprocal Interactions between Polyphenols and Gut Microbiota and Effects on Bioaccessibility Tugba Ozdal 1, David A. Sela 2,†, Jianbo Xiao 3,†, Dilek Boyacioglu 4,†, Fang Chen 5,† and Esra Capanoglu 4,* 1 Department of Food Engineering, Faculty of Engineering and Architecture, Okan Univesity, Tuzla, Istanbul TR-34959, Turkey; [email protected] 2 Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA; [email protected] 3 Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau, China; [email protected] 4 Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, Istanbul TR-34469, Turkey; [email protected] 5 College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, China Agricultural University, Beijing 100083, China; [email protected] * Correspondence: [email protected]; Tel.: +90-212-285-7340; Fax: +90-212-285-7333 † These authors contributed equally to this work. Received: 8 October 2015; Accepted: 11 January 2016; Published: 6 February 2016 Abstract: As of late, polyphenols have increasingly interested the scientific community due to their proposed health benefits. Much of this attention has focused on their bioavailability. Polyphenol–gut microbiota interactions should be considered to understand their biological functions. The dichotomy between the biotransformation of polyphenols into their metabolites by gut microbiota and the modulation of gut microbiota composition by polyphenols contributes to positive health outcomes. Although there are many studies on the in vivo bioavailability of polyphenols, the mutual relationship between polyphenols and gut microbiota is not fully understood.
    [Show full text]
  • 1.3 Gut Microbiome
    TECHNISCHE UNIVERSITÄT MÜNCHEN Lehrstuhl für Analytische Lebensmittelchemie Mass spectrometry based gut meta-metabolomics in obesity and type 2 Diabetes Alesia Walker Vollständiger Ausdruck der von der Fakultät für Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigter Dissertation. Vorsitzender: Univ.-Prof. Dr. E. Grill Prüfer der Dissertation: 1. apl.-Prof. Dr. Ph. Schmitt-Kopplin 2. Univ.-Prof. Dr. M. Rychlik 3. apl.-Prof. Dr. A. Hartmann, (Ludwig-Maximilians-Universität München) Die Dissertation wurde am 14.10.2013 bei der Technischen Universität München eingereicht und durch die Fakultät für Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 25.05.2014 angenommen. TABLE OF CONTENTS Table of contents Table of contents .................................................................................................................................................... I List of Figures ...................................................................................................................................................... IV List of Tables ......................................................................................................................................................... X Abbreviations ...................................................................................................................................................... XI Danksagung
    [Show full text]
  • Biosynthesis of Plant Polyketides in Yeast
    Biosynthesis of plant polyketides in yeast vom Fachbereich Biologie der Technischen Universität Darmstadt zur Erlangung des Grades Doctor rerum naturalium (Dr. rer. nat.) Dissertation von Michael Oliver Eichenberger Erstgutachterin: Prof. Dr. Beatrix Süß Zweitgutachter: Prof. Dr. Heribert Warzecha Drittgutachter: Dr. Michael Næsby Darmstadt 2018 Eichenberger, Michael: Biosynthesis of plant polyketides in yeast Darmstadt, Technische Universität Darmstadt, Jahr der Veröffentlichung der Dissertation auf TUprints: 2019 Tag der mündlichen Prüfung: 26.03.2018 Veröffentlicht unter CC BY-SA 4.0 International https://creativecommons.org/licenses/ Acknowledgments This thesis would not have been possible without the help of many people, therefore I’m deeply grateful to… …Michael for all the guidance, discussions, support, advice, and trust. …Prof. Dr. Beatrix Süß for the great collaboration and co-supersivion. …Prof. Dr. Heribert Warzecha for being co-referee. …Prof. Dr. Johannes Kabisch and Prof. Dr. Eckhard Boles for being on the examination committee. …David for injecting and analyzing thousands of samples. …Rafael, Mounir, Maria, Diane, Caroline, Zina, Yvonne, Wijb, Lara, and Arésu for the great work, enthusiasm, and discussions. …Philipp for his bioinformatics support. …Anders, Sam, Roberta, and Corina for the great discussions, fun times, and good music in the labs. …Beata, Ernesto, Klaas, Niels, Carlos, Nick, and Katherina for the fruitful collaborations across sites. …Vicky for all the plates poured, medias prepared, and the awesome chocolate mousse. …Christophe and the whole AC team for all the debugging. …Stefan for insightful discussions and inputs. …Cristina and Martin for the great Riboswitch work. …The PROMYS consortium for all the inspiration and the great meetings. …Tim, Solvej, and Michael for the collaboration on transcription factors.
    [Show full text]