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Characterisation of the Contribution of the GABA-Benzodiazepine &Alpha

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Characterisation of the Contribution of the GABA-Benzodiazepine &Alpha View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Journal of Cerebral Blood Flow & Metabolism (2012) 32, 731–744 & 2012 ISCBFM All rights reserved 0271-678X/12 www.jcbfm.com Characterisation of the contribution of the GABA-benzodiazepine a1 receptor subtype to [11C]Ro15-4513 PET images James FM Myers1, Lula Rosso2, Ben J Watson1, Sue J Wilson1,3, Nicola J Kalk3, Nicoletta Clementi1, David J Brooks2, David J Nutt3, Federico E Turkheimer2 and Anne R Lingford-Hughes3 1Psychopharmacology Unit, University of Bristol, Bristol, UK; 2Department of Medicine, Centre for Neuroscience, Imperial College London, London, UK; 3Division of Experimental Medicine, Neuropsychopharmacology Unit, Imperial College London, London, UK This positron emission tomography (PET) study aimed to further define selectivity of [11C]Ro15-4513 binding to the GABARa5 relative to the GABARa1 benzodiazepine receptor subtype. The impact of zolpidem, a GABARa1-selective agonist, on [11C]Ro15-4513, which shows selectivity for GABARa5, and the nonselective benzodiazepine ligand [11C]flumazenil binding was assessed in humans. Compartmental modelling of the kinetics of [11C]Ro15-4513 time-activity curves was used to describe distribution volume (VT) differences in regions populated by different GABA receptor subtypes. Those with low a5 were best fitted by one-tissue compartment models; and those with high a5 required a more complex model. The heterogeneity between brain regions suggested spectral analysis as a more appropriate method to quantify binding as it does not a priori specify compartments. Spectral analysis revealed that zolpidem caused a significant VT decrease (B10%) in [11C]flumazenil, but no decrease in [11C]Ro15-4513 binding. Further analysis of [11C]Ro15-4513 kinetics revealed additional frequency components present in regions containing both a1 and a5 subtypes compared with those containing only a1. Zolpidem reduced one component (mean±s.d.: 71%±41%), presumed to reflect a1-subtype binding, but not another (13%±22%), presumed to reflect a5. The proposed method for [11C]Ro15-4513 analysis may allow more accurate selective binding assays and estimation of drug occupancy for other nonselective ligands. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 731–744; doi:10.1038/jcbfm.2011.177; published online 4 January 2012 Keywords: GABA; imaging; kinetic modelling; pharmacokinetics; positron emission tomography Introduction locations in the brain, and their functional implica- tions. For instance, knock-in and knockout genetic Imaging studies of benzodiazepine receptors over the manipulations in mice have shown that the GABA- past 20 years have revealed different levels of benzodiazepine receptor subtype containing the a1 binding in patients with anxiety and panic disorders, subtype is important in sedation and sleep, the a2/3 alcoholism, schizophrenia, epilepsy, and other dis- subtype is involved in anxiety, and the a5 in learning orders (Malizia et al, 1998; Lingford-Hughes et al, and memory (Mo¨hler et al, 2002). In the light of 2010; Busatto et al, 1997; Hammers et al, 2001). these discoveries, it is important to study the role of There is increasing knowledge about the six different these receptor subtypes in humans. Neuroimaging, subtypes of the GABA-benzodiazepine receptor, their in particular positron emission tomography (PET) and single-photon emission tomography (SPET) Correspondence: Professor AR Lingford-Hughes, Division of imaging with receptor-selective ligands, remains Experimental Medicine, Neuropsychopharmacology Unit, Imper- the only way of quantifying benzodiazepine recep- ial College, Burlington Danes Building, Hammersmith Hospital tors in the human brain in vivo. Site, 160 Du Cane Road, London W12 0NN, UK. [11C]flumazenil with PET is the most commonly E-mail: [email protected] used in vivo marker of benzodiazepine receptor This study was supported by MRC Programme Grant (G0400575), binding and its iodinated analogue, [123I]iomazenil, UK Medical Research Council PET Methodology Programme Grant, and MRC/University of Bristol PhD studentship (JFM). can be used with SPET. Flumazenil is an antagonist Received 27 April 2011; revised 16 September 2011; accepted 12 that binds with high affinity to benzodiazepine October 2011; published online 4 January 2012 receptors of the a1, a2, a3, or a5(KiB1 nmol/L) GABARa1 contribution to [11C]Ro15-4513 PET images JFM Myers et al 732 subtypes and with lower affinity to the a4ora6 spectral analysis (Cunningham and Jones, 1993) to subtypes (Ki B150 nmol/L (Sieghart, 1995)). Until both [11C]Ro15-4513 and [11C]flumazenil data sets to recently, there was no way of preferentially visualis- deduce the spectral band of the GABA-benzodiaze- ing particular subtypes. Ro15-4513 shows 10- to 15- pine a1 subtype. The range obtained was used fold selectivity for the a5 subtype over others in vitro to determine the occupancy by zolpidem of the (Ki 0.07 nmol/L versus 1 to 5 nmol/L), and in vivo GABA a1 subtype from the [11C]Ro15-4513 data using [11C]Ro15-4513 PET provides a relatively selective the Lassen plot (Lassen et al, 1995; Cunningham marker for the GABARa5 subtype (Hadingham et al, et al, 2010). 1993; Lingford-Hughes et al, 2002; Maeda et al, 2003). These studies, among others, show that in vivo [11C]Ro15-4513 uptake targets the limbic system, Materials and methods with the anterior cingulate cortex, ventral striatum, This study was approved by a NHS Research Ethics and hippocampus showing particularly high levels Committee, the Administration of Radioactive Substances (Onoe et al, 1996), whereas high [11C]flumazenil Advisory Committee and local NHS Research and Devel- binding is seen in all cortical areas, with highest opment. It was conducted in accordance with Good levels in the occipital cortex. Therefore, the limbic Clinical Practice—International Conference on Harmonisa- pattern of [11C]Ro15-4513 binding is likely to be tion guidelines. relevant to learning, memory, reward, and addiction. For example, we have shown that an inverse agonist at the a5 subtype can reverse the memory-impairing Design effects of acute alcohol consumption (Nutt et al, 2007), and [11C]Ro15-4513 binding is related to There were two separate randomised double-blind placebo- performance on a verbal memory task in alcohol controlled studies, each with six healthy volunteer parti- dependence (Lingford-Hughes et al, 2010). The a5 cipants. The study procedures were identical apart from subtype has also been implicated in the reinforc- the PET ligand used: [11C]flumazenil for one study and ing effects of alcohol in mice (Stephens et al, 2005), [11C]Ro15-4513 for the other. Positron emission tomo- and we have shown a reduction in [11C]Ro15-4513 graphy scans were performed twice for each participant, binding in the nucleus accumbens in alcohol depen- once after zolpidem and once after placebo, with at least a dence compared with healthy controls (Lingford- week between scans. Binding of the ligand was compared Hughes et al, 2010). between the two conditions. Although the distribution of [11C]Ro15-4513 bind- ing is consistent with relatively greater labelling of the a5 subtype, the affinity of [11C]Ro15-4513 is only Subjects an order of magnitude greater for the a5 over other Healthy male volunteers (age mean±s.d.: [11C]Ro15-4513: subtypes. Given the abundance of the a1 subtype 44±6 years; [11C]flumazenil: 43±4 years) underwent 2 throughout the brain, it is important to assess the PET scans at least a week apart, after zolpidem or placebo, contribution of a1 binding to [11C]Ro15-4513 bind- the order of which was randomised. They were all ing. This study compares the binding of [11C]Ro15- screened with urinalysis for illicit drugs of abuse, had no 4513 with that of [11C]flumazenil in the presence and current or previous significant physical or mental dis- absence of zolpidem, a compound with selectivity orders, had taken no psychotropic drugs in the last month, for the a1 receptor subtype that is > 5,000-fold over nor were regular benzodiazepine users. that for the a5 subtype (Hanson et al, 2008; Langer et al, 1992). Unlike classical benzodiazepines, the sedative/hypnotic effect of zolpidem occurs at Zolpidem and Measures much lower doses than the other pharmacological effects attributed to benzodiazepine-site action, such Zolpidem (20 mg) or an identical placebo was administered as anticonvulsant activity and muscle relaxation 90 minutes before radioligand injection and at the start of (Sanger, 2004). the PET scan. Plasma zolpidem levels were measured at the After predosing with zolpidem or placebo, time of PET ligand injection. one group of healthy volunteers was scanned with The following tasks provided a measure of the effect [11C]Ro15-4513 and another group with [11C]fluma- of zolpidem on brain function. Saccadic eye movements zenil. Our hypothesis was that, because [11C]fluma- to a target were measured using electrooculography as zenil labels the a1 subtype, its binding would described previously (Lingford-Hughes et al, 2005). The be reduced by zolpidem. In contrast, we hypo- subject was asked to fixate on a red light as it appeared thesised that [11C]Ro15-4513 binding would not be on the screen mounted 67 cm in front of him. Data were altered by zolpidem in a5-rich regions such as then collected for 48 eye movements of 151 to 401 and a the hippocampus, whereas in a1-rich regions with main sequence curve obtained, the measurement for peak limited or no a5, such
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