ABOUT FRANK BASTIAN, MD

CURRICULUM VITAE: FRANK BASTIAN, MD

HOME ADDRESS: 1132 Peniston New Orleans, LA 70115

WORK ADDRESS: Bastian Laboratory for Neurological Research 2000 Lakeshore Drive University of New Orleans

PERSONAL DATA: born: February 24, 1939, Saskatchewan, Canada American (naturalized)

EDUCATION: University of Saskatchewan Saskatoon, Saskatchewan B.A. 1960

University of Saskatchewan School of Medicine Saskatoon, Saskatchewan M.D. 1964

POST GRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:

1971 – 1972 Research Fellow in Virology (Dr. A. Rabson's Laboratory) National Institutes of Health Bethesda, Maryland l968 - l97l Resident Pathology & Neuropathology Duke University Medical Center Durham, North Carolina

1966 – 1968 General Practice - Canada

1965 – 1966 Resident, General Practice Louisiana State University Lafayette, Louisiana

1964 – 1965 Rotating Intern Charity Hospital Louisiana State University New Orleans, Louisiana Faculty Appointments: 2006 – present Adjunct Research Professor Department of Pathology Tulane Medical School

2006 – 2016 Clinical Professor Departments Neurosurgery & Pathology LSU Medical Center New Orleans, LA

2005 – 2018 Research Professor Department of Animal Science LSU Agriculture Center Baton Rouge, LA

2001 – 2005 Research Professor Department of Pathology Tulane Medical School

1992 – 2001 Professor (Neuropathologist) Department of Pathology University of South Alabama College of Medicine 2451 Fillingim Street Mobile, Alabama 36617

1982 – 1992 Associate Professor of Pathology University of South Alabama

1980 – 1982 Associate Professor of Neuropathology Department of Pathology University of Maryland Baltimore, Maryland

1972 – 1979 Assistant Professor Pathology and Neuropathology Baylor Medical Center Staff Pathologist St. Luke's Episcopal Hospital Texas Medical Center Houston, Texas

Specialty Certification: Board Certified in Anatomic Pathology & Neuropathology, 1972

Licensure: Alabama

Awards, Honors, and Memberships in Honor Societies:

July 1 - Aug 1, 1984 Dr. A. C. Palmer's Laboratory, The Welcome Laboratory of Comparative Neurology,Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, England

University of South Alabama Animal Care & Use Committee/1985 Radiation Committee /1986 Institutional Self-Study Subcommittee 1987 Faculty Senate 1989-1996 Chair, COM Caucus - 1993-4, 1994-5 Executive of Faculty Senate Chair, Faculty Handbook Committee 1994-5, 1995-6 Biosafety committee 1992-1993

NATIONAL AWARD committee, 1981, American Association of.Neuropathology.

2002 - 2003 Reviewer (Ad hoc chairman) Army Program

Membership in Professional and Scientific Societies:

American Association of Neuropathologists International Academy of Pathologists Duke Alumni Association New York Academy of Science American Association of Pathologists American Society of Microbiology International Organization for Mycoplasmology

Scientific Journal Ad-hoc Reviewer

Journal of Neuropathology and Experimental Neurology Southern Medical Journal Archives of Internal Medicine American Journal of Rheumatology TESTIMONY BEFORE THE CONGRESSIONAL SUBCOMMITTEE ON HUMAN RESOURCES

"Government Reform Committee To Convene Hearing On FDA Steps Against Mad Cow Disease And Related Diseases" Washington DC, January 29, 1997

Scientific Grant Review Sections:

Ad Hoc Reviewer for National Science Foundation Grant , DCB-8903813 (March 1989) Ad Hoc Reviewer for Army Prion Project

Past Research Funding 1984 – 1986 RFP - NINCDS-84-07 "The Use of a University of South AL Tetrahydrobiopterin (BH4) Analog for the Treatment of Parkinson's Disease and Other BH4 Deficiency Disorders". ($387,099.00)

1985 – 1986 Ciba-Geigy Parkinson's Disease - Drug Study University of South AL ($120,000.00)

1986 – 1987 Ciba-Geigy "Experimental Model of MPTP University of South AL Induced Parkinson's Disease in the Squirrel Monkey" ($100,000.00)

1998 - 2001 Baxter HealthCare Corp "Baxter-CJD University of South AL Squirrel Monkey Study" ($1,651,324) per year for 5 years

1998 National Heart, Lung & Blood National University of MD Institutes of Health "Study of role of in the Transmissible Spongiform Encephalopathies" ($40,000)

AS COLLABORATING INVESTIGATOR l990 - l99l DDR-BRS Shared Instrumentation Grant Project l6 "Characterization of Intracellular Infection Using the Neurobastoma Cell Line, Neuro-2a" ($290,495)

1981 – 1982 Paghorn Fund University of MD "Role of Spiroplasma Infection in Spongiform Encephalopathy" $1,500.00 for supplies 1982 – 1985 NIH Grant # 1RO1 NS19596-01 University of South AL "Role of Spiroplasma Infection in Spongiform Encephalopathy" 20% effort 3 years ($290,739.00)

1983 – 1985 Supplemental to NIH Grant #1 RO1 Unversity of South AL NS19596-01 ($12,000.00)

1998 Abbott Labs “Spiroplasma/CJD Study" University of South AL ($15,000)

2001 – 2007 NIH Grant # R01 NS44000-03 Tulane Medical School “Characterization of Spiroplasma 16S rDNA In TSE by PCR and Direct Sequence Analysis” ($375,000) Extension 2006 – 2007 7 R01 NS44000-03 ($ 50,700.00)

2008 – 2009 BAIT grant- LSU Agricultural Center (30,000)

2008 – 2010 with extension National Science Foundation/USDA to 2012 Whole genome sequencing and annotation of representative strains of ’ 15% effort (376,320.00)

Invited Lectures

1. Recent Advances in the Classification of Gliomas. Presented to the Houston Society of Clinical Pathologists, May, 1979

2. Application of Immunological Methods to the Diagnosis and Page Therapy of Tumors. Presented to St. Luke's Epsicopal Tumor Registry, October, 1979.

3. Creutzfeldt-Jakob Disease - Possible Causal Mechanisms. Presented to Maryland Society of Pathologists, February, 1981.

4. Possible Role of Spiroplasma in Creutzfeldt-Jakob Disease. Presented to American Society of Bacteriologists Maryland Branch, April, 1981.

5. Spiroplasma and Creutzfeldt-Jakob Disease. University of Saskatchewan, Saskatoon Saskatchewan, Canada, June, 1981.

6. Spiroplasma: A new look at Creutzfeldt-Jakob Disease. Armed Forces Institute of Pathology Seminar, Washington, D.C., July 1981.

7. Spiroplasma and the spongiform encephalopathies. Presented to Philadelphia Neurological Society, Philadelphia, PA, September, 1981. 8. The Classification of Astrocytoma. Presented at the Maryland Society of Pathologists, November, 1981.

9. New approach to the transmissible dementias. Presented at the annual meeting of the Alabama Association of Neurosurgeons, Grand Hotel, Point Clear, AL, June 3, 1983.

10. Organic Dementias-with Discussion of Possible role of Spiroplasma in Creutzfeldt-Jakob Disease. Presented at the annual meeting of the Mississippi Association of Neurosurgeons, Biloxi, MS, May 13-14, 1983.

11. Creutzfeldt-Jakob Disease - recent evidence indicating association of Spiroplasma infection. Visiting Professor, Veterans Administration Hospital, Biloxi & Gulfport, MS, September 21, 1983.

12. Primary brain tumors - with particular reference to Astrocytomas. Visiting Professor, Veterans Administration Hospital, Biloxi, MS, September 22, 1983.

13. Creutzfeldt-Jakob disease - new concepts in pathogenesis. Univ of Cincinati Med Cntr, Nov, 1983.

14. Creutzfeldt-Jakob disease & spiroplasma infection. Cincinati Assoc of Pathologists, Nov, 1983

15. Creutzfeldt-Jakob disease. Cleveland clinic, Nov, 1983

16. and Spiroplasma Associated Fibrils. Neuropathologenicity Unit, West Maine's Road Edinborough, England, August 1, 1984.

17. Spiroplasma Induction of Spongiform Encephalopathy. John Innes Institute, Agriculture & Food Research Council, Colney Lane, Norwich, August, 1984.

18. Creutzfeldt-Jakob Disease and Spiroplasma Infection. Institute of Neurology, Queen's Square House, London, England, August, 1984.

19. Scrapie, Creutzfeldt-Jakob Disease and Spiroplasma. Clinical Research Center, Medical Research Council, Northwick Park Hospital, Wadford Road, Harrow, London, August, 1984.

20. Slow Brain Infections in Animals Role of Spiroplasma. The Welcome Laboratory of Comparative Neurology, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, England, August, 1984.

21. New Aspects of Creutzfeldt-Jakob Disease. USA College Wide Forum. Mobile, AL, December 20, 1984.

22. Recent Advances in Dementia and Creutzfeldt-Jakob Disease. Psychiatric Grand Rounds. Mobile, AL, June 16, 1985.

23. New Concepts of Dementia. Alzheimer's Support Group. Pensacola, FL, July 11, 1985

24. Creutzfeldt-Jakob Disease: An update. Searcy Institute of Mental Health. Mt Vernon, AL, July 30, 1985.

25. Recent Developments in Transmissible Dementia. Presented at the Third Annual Alzheimer's Symposium, Gatlinburg, TN, Nov, 1986.

26. Peripheral Nerve Tumors. Presented at Neuropathology symposium, Sponsored by AL society of pathology Oct 30,1987

27. Recent Developments in Creutzfeldt-Jakob disease. Guest lecturer, University of Florida, Gainsville, FLA, August, 1990.

28. Current Aspects in Research on Creutzfeldt-Jakob disease. USA Neurology/Neurosurgery grand rounds, July, 1990.

29. Creutzfeldt-Jakob disease New advances in understanding of pathogenic mechanisms. Univ MO Med Cntr, Kansas city, MO, March, 1991.

30. Classification of Astrocytomas (the pilocytic astrocytomas). Children's hospital, Univ of MO Med Cntr, Kansas city MO, March, 1991.

31. Dementia. Psychiatric & extended care cntr, Univ of MO, Kansas city MO, March, 1991.

32. Ocular disease. Univ of MO Med Cntr, Kansas city MO, March 1991.

33. Neuropathology of AIDS. USA Neurology/Neurosurgery grand rounds, April, 1991.

34. Role of spiroplasma in Creutzfeldt-Jakob disease. Armed Forces Institute of Pathology, Washington DC, June, 1991.

35. Spiroplasma and Honey . Mobile Association of keepers, August, 1993.

36. Spiroplasma as dormant microbes. Invited seminar (AAM round table) on Dormant microbes presented at 94th General meeting of the ASM. Las Vegas, May 27, 1994.

37. Possible Causes of Infectious Dementia. Invited faculty Highlights in Medicine Conference, Saskatoon, SASK, Canada, June 24, 1994.

38. Role of Spiroplasma in Pathogenesis of Creutzfeldt-Jakob Disease. Visiting Professor, University of Saskatchewan College of Medicine, Department of Pathology, June 23, 1994.

39. Creutzfeldt-Jakob disease as a Potential Health Problem. Mobile County Health Department, July, 1994.

40. Creutzfeldt-Jakob disease- Epidemiological considerations. Alabama State Health Department, Montgomery AL, July, 1994.

41. Current Status on Creutzfeldt-Jakob disease. Visiting Professor, Baxter Biotech, Duarte, CA, Dec, 1995.

42. , Bovine Spongiform Encephalopathy ("Mad Cow Disease") and Human Health Risk. The Richard J. Duma/NFID Annual Press Conference on Infectious Diseases, The National Press Club, Washington, D.C., May 16, 1996.

43. Pathogenic mechanisms in Creutzfeldt-Jakob disease. The National advisory committee on BSE/scrapie, Aimes, Iowa, June10-11, 1996.

44. Pathogenic mechanisms in Creutzfeldt-Jakob disease. Southwest Alabama Medical Center, Dothan, AL June, 1996.

45. Pathogenic mechanisms in the transmissible spongiform encephalopathies, International symsposium on Spongiform Encephalopathies, The Ceres Forum, Georgetown University, Washington, DC, December 12, 1996.

46. Spiroplasma and the Transmissible Spongiform Encephalopathies NMHCC's 2nd International Conference on TSE Washington, DC, Nov 17-18, 1997.

47. Spiroplasma and the Transmissible Spongiform Encephalopathies NMHCC's 3rd International Conference on TSE San Diego CA, Feb, 1998.

48. Spiroplasma and the Transmissible Spongiform Encephalopathies CJD Voice conference on CJD, Bloomington, Indiana, June 15, 1999.

49. Role of Spiroplasma in the Pathogenesis of the Transmissible Spongiform Encephalopathies 1st Conference of the CJD Foundation Inc. Miami, Florida, May 7-8, 2000

50. Demonstration of Spiroplasma DNA in TSE tissues. Visiting Professor, Tulane University School of Medicine, May 15, 01.

51. Association of Spiroplasma with TSE. Visiting Professor, Joy Goodwin Lecturer, Auburn Veterinary College, June 14, 01.

52. Role of Spiroplasma in TSE. Visiting Professor. Pennington Institute, LSU , June 19, 2001.

53. Spiroplasma in Chronic Wasting disease. Invited speaker. Elk Breeder's Association annual meeting. Kansas city, Kansas, July 27, 03

54. New horizons in TSE research. Invited Speaker. Symposium on Chronic Wasting disease. Cervid Council of Canada, Ottawa, Ont. CA, Oct 8/03.

55. Spiroplasma and the transmissible spongiform encephalopathies- an update. LSUHSC at Shreveport July/04

56. Role of Spiroplasma in TSEs Elliot Foundation, Mississippi, October, 2007

57. BACTERIAL PATHOGENESIS OF THE TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES, BETTER KNOWN AS ‘MAD COW DISEASE’ lecturer at the Western College of Veterinary Medicine (U of S) Robert & Virginia Rausch Visiting Professorship, October 26/2010.

58. SPIROPLASMOSIS AND THE TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES- VIRULENCE FACTORS, lecturer at the Western College of Veterinary Medicine (U of S) Robert & Virginia Rausch Visiting Professorship, October 26/2010

59. Spiroplasmosis in the pathogenesis of the Transmissible Spongiform Encephalopathies. Tulane Medical School, Pathology Grand Rounds, June 6/2011

60. Spiroplasma as pathogens in human and animal diseases. Veterinary Laboratories Agency, Weybridge, UK, June 23/2011

61. Spiroplasmosis and the Transmissible Spongiform Encephalopathies. LSUHSC Pathology grand rounds, October 11/2011

62. Is AD infectious. 2015 Alzheimer Disease Congress. London, UK June 23, 2015

63. Isolation of Spiroplasma sp. from TSE, International Organization of Mycoplasmology New Hampshire July, 2018

64. Refinement of test for Spiroplasma sp. in TSE. LSU-ACRES Research in Endangered Species Symposium. August 2018

65. Role of Spiroplasma in CWD. Deer Breeder’s National Meeting. Indiana April 2018

66. Recent breakthrough in investigating the role of a novel Spiroplasma sp. in CWD

67. Role of Spiroplasma sp. in CWD. Deer Breeder Meeting, Texas 2018

68. Spiroplasma and CWD- breakthrough in research. Deer breeder Organization (Canadian) Saskatoon Saskatchewan, 2018

Bibliography

Original Manuscripts, letters and commentaries:

1. Bastian FO, Parker JC: Multicentric gliomas. Amer J Clin Pathol 0;54:839- 844.

2. Bastian FO: Papova-like virus particles in a human brain tumor. Lab Invest 971;25:69-175.

3. Bastian FO: Amyloidosis and the carpal tunnel syndrome Amer J Clin Pathol 974;61:611-717.

4. Bastian FO: Amyloidosis and the carpal tunnel syndrome. Yearbook of Orthopedics and Traumatic Surgery. (Ed) H Herman Young, 1975.

5. Bastian FO, Rabson AS, Yee CL, Tralka TS: Herpes virus hominis: Isolation from human trigeminal ganglion Science l972;178:306-307.

6. Bastian FO, Rabson AS, Yee CL, Tralka TS: Herpes virus varicellae: Isolation from human dorsal root ganglia. Arch Pathol l974;97:331.

7. Bastian FO, Singer D, Wendy R, Zeller R: Eastern equine encephalitis histopathologic and ultrastructural changeswith isolation of the virus in a human case. Amer J Clin Pathol l975;64:10-13.

8. Van Horn G, Bastian FO, Moake J: Progressive multifocal leukoencephalopathy: Failure of response to transfer factor and cytarbine. Neurology l978;28:794-797.

9. Middleditch P. Minard B. Crawley L. Bastian FO: Horseeradish peroxidase labeled antisera: A diagnostic method for evaluating the percentages of B- lymphocytes in peripheral blood. J Histochem Cytochem l979;27:689-690.

10. Bastian FO: Spiroplasma-like inclusions in Creutzfeldt-Jakob disease. Arch Pathol Lab Med l979;103:665-669.

11. Bastian FO, Middleditch P, Bossart M: A method for enumeration of T- and B- lymphocytes in lymphoid tissues. J Histochem Cytochem l979;27:1343-1345.

12. Alpert JN, Groff A, Bastian FO, Blum MA: Acute polymyositis caused by sarcoidosis. Report of a case and review of the literature. Mt Sinai J Med l979;46:486-488. 13. Schmidt W, Webb J, Bedrossian C, Ali V, Bastian FO: Actinomycosis and intrauterine contraceptive devices. Diagnostic Gyn Obst l980;2:165-177.

14. Hines HB, Middleditch BS, Bastian FO, Middleditch PR: 2,5-di-tertamyl-o-benzoquinone: An antioxident encountered during analysis of human tissues. Biomed Mass Spectrom l98l;l7:ll05-lll5.

15. Hayman LA, Evans RA, Bastian FO, Hinck VC: Delayed high dose contrast T: identifying patients at risk of massive hemorrhagic infarction. Amer J Radiol l981;136:1151-1159.

16. Bastian FO, Hart MN, Cancilla PA: Additional evidence of Spiroplasma in Creutzfeldt-Jakob disease. Lancet l981;1(8221):660.

17. Nirankeri V, Karrash JE, Bastian FO, Billing E: Recurrence of keratoconus in donor cornea 22 years following successful keratoplasy. Brit J Opthal l983;67:23-28.

18. Bastian FO, Jennings RA: Creutzfeldt-Jakob Disease: Procedures for Handling Diagnostic and Research Materials. Infect Control l983;5(1):48-50.

19. Bellis EH, Salcman M, Bastian FO: Primitive neuroectodermal tumor in a 57- year old man. Surg Neurol l983;20:30-35.

20. Bastian FO, Purnell DM, Tully JG: Neuropathology of spiroplasma infection in the rat brain. Amer J Pathol l984;114(3):496-514.

21. Tully JG, Bastian FO, Rose DL: Localization and Persistence of Spiroplasma in an Experimental Brain Infection in Suckling Rats. Ann Microbiol (Inst Pasteur) 84;135A:111-117.

22. Superneau DW, Wertelecki W, Zellweger H, Bastian FO: Myopathy in Marinesco-Sjogren syndrome. Eur J Neurol l987;26:8-16.

23. Bastian FO, Jennings R, Gardner W: Antiserum to Scrapie Associated Fibril Protein Cross-Reacts with Spiroplasma mirum Fibril Proteins. J Clin Microbiol l987;25(12):2430-2431.

24. Bastian FO, Jennings R, Huff C: Neurotropic Response of Spiroplasma mirum following peripheral inoculation in the rat. Ann Microbiol (Inst Pasteur) 1987;138(6):651.

25. Nelson GA, Schlitt M, Bastian FO: Malignant Transformation in Craniopharyngioma. Neurosurgery l988;22(2):427-429.

26. Schlitt M, Bucher A, Stroop WG, Pindak F, Bastian FO, Jennings R, Lakeman AD, Whitley RJ: Neurovirulence in an experimental focal Herpes Encephalitis: relationship to observed seizures. Brain Res l988;440:293-298. 27. Bastian FO, Baliga BS, Pollock HM: Evaluation of a modified [3H]thymidine uptake method for studying growth of Spiroplasmas under varying conditions. J Clin Microbiol l988;26(10):2124-2126.

28. Maertens P, Richardson R, Bastian FO, Williams JP, Hommes F:A new type of mitochondrial encephalomyopathy with stroke-like episodes due to cytochrome oxidase deficiency. J Inher Metab Dis l988;11[Suppl 2]:186-188.

29. Bastian FO, Jennings RA, Hoff CJ: Effect of Trimethoprim/Sulphasoxazole and hyperbaric oxygen on experimental Spiroplasma mirum encephalitis. Ann Microbiol (Inst Pasteur) l989;l40:l5l-l58.

30. Schlitt M, Williams JP, Bastian FO, Smith S: The small midline occipital encephalomeningocele: definition of a syndrome. J Neurosurg l989;24(4):2404-2406.

31. Titford M, Bastian FO: Handling CJD Tissues in the Histology Laboratory. J Histochem l989;l2(3):2l4-2l7.

32. Schlitt M, Bucher AP, Quindlin EA, Jennings R, Bastian FO: Nonfulminate Herpes Simplex encephalitis. Med Hypothesis 1990;33:177-179.

33. Bastian FO: Historical review of Creutzfeldt-Jakob disease. In FO Bastian (ed.), Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies, Mosby/Year Book, 1991, pp. 1-7.

34. Bastian FO: Neuropathology of Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies. In FO Bastian (ed.), Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies, Mosby/Year Book, 1991, pp. 65-96.

35. Bastian FO, Quindlin E: Ethical and legal aspects of Creutzfeldt-Jakob disease. In FO Bastian (ed.), Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies, Mosby Co/Year Book, 1991, pp. 224-238.

36. Bastian FO: Treatment of Creutzfeldt-Jakob disease and experimental transmissible spongiform encephalopathies. In FO Bastian (ed.), Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies, Mosby/Year Book, 1991, pp. 213-223.

37. Bastian FO: Review of the theories on the nature of the transmissible agent/s of Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies. In FO Bastian (ed.), Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies, Mosby/Year Book, 1991, pp. 49-64.

38. Cookston M, Cotter G, Schlitt M, Bastian FO.: Primary Angiosarcoma of the Brain. A Case Report and Review of the Literature. So Med J 1991;83:517-520.

39. Maertens P, Snow R, Boudreaux C, Bastian FO, Weber E: Neuroimaging CPC: Backache, unsteady gait, incontinence, and larger thoracic epidural mass. J Neuroimaging 1993;3:184-189.

40. Bastian FO: Bovine Spongiform Encephalopathy: relationship to human disease and nature of the agent. ASM news 1993;59:235-240.

41. Ferrigno M, Bishop B, Impastato K, Brady AG, Titford M, Bastian FO: Transvenous phrenic nerve stimulation during prolonged mechanical ventilation in awake, non-paralyzed ewes, Lab Animal Sci 1995;45:690-693.

42. Lolley RD, Bose WJ, Bastian FO, Bassam B, Meyer FN, Anderson LD: Vein, Silastic and PGA fine mesh: comparative study in peripheral nerve repair. Ann Plastic Surg, 1995;45:266-277.

43. Kepes JJ, Bastian FO, Weber ED: Gliosarcoma developing from an irradiated Ependymoma case report. Acta Neuropathol 1996;92:515-519

44. Brown P, Cervenáková, Goldfarb LG, Bishop K, Bastian FO, Kirkpatrick J, Piccardo P, Ghetti B, Gajdusek DC. Creutzfeldt-Jakob disease in a husband and wife. Neurology 1998;50:684-688.

45. Bastian FO, Foster J: Spiroplasma 16S rDNA in Creutzfeldt-Jakob disease and Scrapie as Shown by PCR and DNA Sequence Analysis. J Neuropathol Exp Neurol 2001;60:613-620.

46. Qi Z, Kalkeri G, Hanible J, Prabhu R. Bastian FO, Garry RF, Dash S. Stemloop structures(II-IV) of the 5’ untranslated sequences are required for the expression of the full-length hepatitis C virus genome. Arch Virol 2003;147:449-467.

47. Akhter S, Liu H, Prabhu R, DeLuca C, Bastian FO, Garry RF, Thung SN, Dash S. Epstein Barr virus and human hepatocellular carcinoma. Cancer Let 2003;192:49-57.

48. Bastian FO, Dash S, Garry R. Linking chronic wasting disease to scrapie by comparison of Spiroplasma mirum ribosomal DNA sequences. Exp Mol Pathol 2004;77:48-56.

49. Bastian FO, McDermontt ME, Perry AS, Carver LA., Dash S, Garry RF. Safe method for isolation of prion protein and diagnosis of Creutzfeldt-Jakob disease. J Virol Methods 2005;130: 133-139.

50. Bastian FO. Spiroplasma as a candidate causal agent of transmissible spongiform encephalopathies. J Neuropathol Exp Neurol 2005;64: 833-838.

51. Bastian FO, Fermin CD. (2005) Slow virus disease: Deciphering conflicting data on the transmissible spongiform encephalopathies (TSE) also called prion disease https://doi.org/10.1002/jemt.20223

52. Bastian FO, Sanders DE, Forbes WA, Hagius SD, Walker JV, Henk WG, Enright FM, Elzer PH. Spiroplasma spp. from TSE Brains or Ticks Induce Spongiform Encephalopathy in Ruminants. J Med Microbiol, 2007; 56: 1235-1242.

53. Bastian FO, Boudreaux C, Hagius SD, Sorensen S, Bulgin M, Enright FM, Elzer PH. Spiroplasma found in eyes of scrapie affected sheep. Veterinary Ophthalmology (2011) 14, 1, 10– 17

54. Bastian FO, Elzer PH, Wu X (2012) Spiroplasma spp. biofilm formation is instrumental for their role in the pathogenesis of plant, insect and animal diseases. Exp Mol Pathol 93: 116-128.

55. Bastian FO. (2014) The case for involvement of spiroplasma in the pathogenesis of transmissible spongiform encephalopathies. J Neuropathol Exp Neurol 73: 104-114

56. Bastian FO (2014) Cross-roads in research on neurodegenerative diseases. J Alzheimer’s Dis & Parkinsonism 4: 141. doi:10.4172/21610460.1000141.

57. Bastian FO. (2017) Combined Creutzfeldt-Jakob/ Alzheimer’s Disease Cases are Important in Search for Microbes in Alzheimer’s Disease. Journal of Alzheimer's Disease, 56: 867-873.

58. Bastian FO, Lynch J, Hagius S, Wu X, McCormick G, Luther DG, Elzer PH. (2018) Novel Spiroplasma Spp. Cultured From Brains and Lymph Nodes From Ruminants Affected With Transmissible Spongiform Encephalopathy Journal of Neuropathology & Experimental Neurology, 77: 64-73.

Books:

Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies. Edited by: Frank O. Bastian, MD, Professor, Department of Pathology, College of Medicine, University of South Alabama. Mosby/Year Book, March 1991. Abstracts:

1. Bastian FO, Parker JC: Multicentric gliomas. Presented to International Association of Pathology Meeting. St Louis Missouri, March, 1970.

2. Bastian FO: Papova-like virus particles in a human brain tumor. Presented to American Association of Neuropathologists. San Juan, Puerto Rico, June 1971.

3. Bastian FO: Spiroplasma-like inclusions in Creutzfeldt-Jakob disease. Presented in part at VIII International Congress of Neuropathology, Washington, DC, September, 1978.

4. Schmidt W, Webb J, Bedrossian C, Ali V, Bastian FO: Actinomycosis and intrauterine contraceptive devices. Presented in part to Texas Medical Association Meeting. Dallas, Texas. April, 1979.

5. Hines HB, Middleditch BS, Bastian FO, Middleditch PR: 2,5-di-tert-amyl-o-benzoquinone: An antioxidant encountered during analysis of human tissues. Presented in part to American Society for Mass Spectrometry. Seattle, Washington, May, 1979.

6. Dennis D, Neblett CR, Bastian FO: Collision tumors: Association of meningioma and oligodendroglioma. Presented in part at Rocky Mountain Neurological Society, 1979.

7. Peters M, Bastian FO, Moiel R, Singer D: Choroid plexus papilloma of the third ventricle. Presented in part at Rock Mountain Neurological Society, 1974, pp 6.

8. Newman AJ, Carlton CE, Graham CE, Bastian FO: Blood group isoantigen deletion in carcinoma in situ and atypical epithelial hyperplasia of the urinary bladder. Presented in part to the National American Urological Association in San Francisco. May, 1980.

9. Hayman LA, Evans RA, Hinck VC, Bastian FO: CT detection of blood brain abnormality. Presented to the Radiological Society of North America. Atlanta, Georgia, November,1979.

10. Bastian FO, Tully JG: Morphological aspects of experimental Spiroplasma infection in the rat brain. Presented in part to the American Association of Neuropathologists in Vancouver, BC, Canada, June, 1981.

11. Hayman LA, Frotta JC, Bastian FO, Hinck VC: The clinical significance of CT contrast enhancement 2 to 150 days after cerebral infarction. Presented at Computed Tomography International Symposium and Course. New Orleans, LA, April, 1981.

12. Nirankeri V, Karrash JR, Bastian FO, Billing JE: Recurrence of keratoconus in donor eye following successful keratoplasty. Presented at Eye Bank Association Meeting, Atlanta, Georgia, September 1981.

13. Tully JG, Rose, Bastian FO: Localization and Persistence of Spiroplasma in experimental brain infection. Presented in part to the International Symposium on Pathogenicity of , Bordeaux, France, July 8-11, 1983.

14. Bastian FO, Purnell DM, Tully JG: Neuropathology of spiroplasma infection in the rat brain. Presented in part to the American Association of Neuropathologists. St Louis, Missouri, June 9-12, 1983.

15. Edathoudu AK, Bastian FO, Dyken PR, Jennings D, Sindel L, Maertens P: Lymphocytic ultrastructural in studies on neuronal ceroid-lipofuscinosis (NCL) using a ficoll gradient technique. AAN Scientific Program, October, 1984.

16. Bastian FO, Jennings RA, Quindlen E: Spiroplasma-associated Fibrils. J Neuropathol Exp Neurol l984;43:333.

17. Jennings R, Bastian FO: Spiroplasma: Properties similar to the infectious agent of scrapie and Creutzfeldt-Jakob disease. Ann Clin Lab Sci l984;14:(4)314.

18. Bastian FO: Properties of Spiroplasma. Canadian Association of Neuropathologists. Ottawa, Canada, 1984.

19. Edathoudu AK, Bastian FO, Dyken P, Jennings DA: Diagnosis of neuronal ceroid lipofuscinosis by lymphocyte ultrastructure. Alabama Electron Microscopy Society, Mobile, AL, March, 1985.

20. Bastian FO, Jennings R, Ryan E: Chemical susceptibility and heat stability of pathogenic Spiroplasma. Am Soc Microbiol, Las Vegas, Nevada, March 7, 1985.

21. Ashburn MA, Jennings R, Bastian FO: Experimental Spiroplasma infection in the rat: survivorship enhancement through hyperbaric oxygen and antibiotic therapy. 11th Annual Gulf Atlantic Residents Conf, Richmond, VA, 1985.

22. Ashburn MA, Jennings R, Bastian FO: Application of hyperbaric oxygen and antibiotic therapy for experimental Spiroplasma infection in the rat. Joint Undersea Medical Society Conf, Long Beach, CA, 1985.

23. Jennings R, Ashburn MA, Bastian FO: Hyperbaric oxygenation and sulfonamides effect on experimental Spiroplasma infection. Am Soc Microbiol, Washington, DC l986.

24. Bastian FO, Titford M, Jennings RA: Creutzfeldt-Jakob Disease and Spiroplasmosis: A Golgi Study. 6th International Congress of IOM. Birmingham1986, pp 114.

25. Bastian FO, Jennings RA: Spiroplasma Fibrils-Morphological and Biochemical Similarities to Fibrils Isolated from Creutzfeldt-Jakob Disease and Scrapie. 6th International Congress of IOM. Birmingham, 1986;p 115.

26. Jennings RA, Bastian FO: Neurotropic Properties of Spiroplasma. 6th International Congress of IOM. Birmingham, 1986, pp 116.

27. Jennings RA, Ryan EM, Bastian FO: Chemical Susceptibility and Heat Stability of Cultivable Spiroplasma. 6th International Congress of IOM. Birmingham, 1986, pp 117.

28. Jennings RA, Ashburn MA, Bastian FO: Effect of Hyperbaric Oxygenation and Sulfonamides on Experimental Spiroplasma Infection in the Suckling Rat. 6th International Congress of IOM. Birmingham, 1986, pp 118.

29. Schlitt M, Wilson E, Whitley, Bastian FO: Topographic and morphologic correlates in a rabbit model of Herpes simplex encephalitis. FASEB, 1986.

30. Maertens P, Richardson R, Bastian FO, Hommes F: A new type of mitochondrial encephalopathy with stroke-like episodes due to cytochrome oxidase deficiency. Society for the Study of inborn errors of . Sheffield, England, September, 1987.

31. Bastian FO, Baliga B, Pollock HM: [3H]Thymidine Uptake for Determination of the Susceptibility of Spiroplasma to Antibiotics. l0th International Congress of IOM. Turkey, l990, pp 555.

32. Metcalf JE, Christianson MD, Oakes JE, Brady AG, Bastian, FO: Herpes Zoster Ophthalmicus in the African Green Monkey: A new animal model. International Conference on Herpetic Eye Diseases, Oxford University, Oxford, England, l990.

33. Bomberger C, Bastian FO, Clement W: Eukaryotic chromosome abnormalities secondary to spiroplasma infection. 17th annual Med Stud Res USA, Aug 1990.

34. Bastian FO, Bomberger C, Clement W: Eucaryotic chromosome abnormalities secondary to spiroplasma infectioni. ASM, Dallas TX, May 1991

35. Bastian FO, Baliga BS, Pollock HM: [3H]thymidine uptake for determination of the susceptibility of spiroplasma to antibiotics. submitted to Amer Assoc of Microbiol, May/92

36. Bastian FO, Clement WM, Holbrook WM: Eucaryotic chromosome abnormalities secondary to spiroplasma infection. IOM lett 1992;2:142.

37. Bastian FO, Davis C, Tuck-Muller: Effect of spiroplasma infection on human fibroblast chromosomes. ASM 93rd General meeting Abstracts P 163

38. Maertens P, Vuk S, Bastian FO, Martinez J: Fat soluble vitamins: deficiency in Zellweger syndrome. Abstracts of free communications. 30th annual symp SSIEE, LEUVEN 1992 abstract 164.

39. Williams JP, Snow RD, Kasmia A, Bastian FO: Primary meningeal sarcoma. South East Neuroradiol Soc 16 annual meeting

40. Snow RD, Williams JP, Maertens P, Boudreaux C, Bastian FO: Rasmussen sydrome: MRI and histologic features. South East Neuroradiol Soc 16 annual meeting

41. Ferrigno M, Bishop B, Brady A, Bastian F, Titford M: Transvenous phrenic nerve stimulation in awake ewes during prolonged mechanical ventilation. PEEP Soc meeting, Miami FL

42. Bastian FO: Superantigens in Creutzfeldt-Jakob disease Pathogenic mechanism in proposed spiroplasma infection. 11th international Congress of the International Organization of Mycoplasmology, Orlando FL, July 16, 1996.

43. Bastian FO, Pittler S, Foster J: Spiroplasma 16S RNA Gene in Transmissible spongiform encephalopathies. J Neuropathol Exp Neurol 1998;57:493.

44. Bastian FO, Pittler S, Foster J: Spiroplasma 16S RNA Gene in Transmissible spongiform encephalopathies. ASM General Meeting, Atlanta GA, May, 1998.

45. Chauker TL, Bastian FO: Polymicrogyria in a 6 year old male. 93rd Annual Scientific Assembly of the Southern Medical Association. Nov 10-14, 1999, Dallas, TX. S. Med. J. 1999;92:570.

46. Martin BL, Tucker AJ, Bastian, FO: Metastatic sarcomatoid carcinoma of the lung mimicking a primary brain tumor. 93rd Annual Scientific Assembly of the Southern Medical Association. Nov 10-14, 1999, Dallas, TX. S. Med. J. 1999;92:S100.

47. Bastian FO, Foster J: Charaterization of Spiroplasma 16S rDNA in TSE by direct DNA sequence analysis. ASM General Meeting, Orlando, FL, May, 2001

48. Bastian FO, Foster J: Demonstration of Spiroplasma 16S rDNA in TSE tissues by Direct DNA sequence analysis. J Neuropathol Exp Neurol 2001;60:555.

49. Bastian FO, Sanders DE, Forbes WA, Hagius SD, Walker JV, Henk WG, Enright FM, Elzer PH. Experimental spiroplasma infection in deer: an animal model for CWD. International congress of Neuropathology, Sept 2006

50. Bastian FO, Sanders DE, Forbes WA, Hagius SD, Walker JV, Henk WG, Enright FM, Elzer PH. Spiroplasma spp. Isolated from Rabbit Ticks or TSE-affected Brains Induce Spongiform Encephalopathy in Ruminants. AANP April 26-30, 2007 Washington DC

51. Bastian FO, Sanders DE, Forbes WA, Hagius SD, Walker JV, Henk WG, Enright FM, Elzer PH. SPIROPLASMOSIS IN RUMINANTS SHOWS REMARKABLY SIMILIAR PATHOLOGIES TO 'PRION DISEASE' 9th European Congress of Neuropathology (8- 10 May 2008) Amsterdam.

52. Bastian FO, Todd W, Boudreaux C, Enright F, Elzer, P. Spiroplasma induced retinopathy in ruminants. Experimental Biol Conference, San Diego, April 5-7, 2008

53. Bastian FO, Boudreaux CM, Hagius SD, Bulgin MS, Sorensen-Melson SJ, Enright FM, Elzer PH. SPIROPLASMOSIS FOUND IN SHEEP FROM SCRAPIE RESEARCH HERD MICROBIAL PATHOGENESIS & HOST RESPONSE September 8 – 12, 2009 Cold Spring Harbor Meeting

54. Bastian FO, Boudreaux CM, Hagius SD, Bulgin MS, Sorensen-Melson SJ, Enright FM, Elzer PH. Eye findings in experimental spiroplasmosis simulating scrapie. AANP, Philadelphia, PA June 12, 2010

55. Bastian FO, Boudreaux CM, Hagius SD, Bulgin MS, Sorensen-Melson SJ, Enright FM, Elzer PH. Spiroplasma found in eyes of sheep with scrapie shows causal role. 18th Congress of the International organization of Mycoplasmology, Chianciano Terme, Italy July 11- 16, 2010

67. Bastian FO, Boudreaux CM, Hagius SD, Bulgin MS, Sorensen-Melson SJ, Enright FM, Elzer PH. Spiroplasmosis induced eye changes in ruminant TSE model suggests retinopathy as an early clinical sign of CJD. 135th Annual Meeting of the American Neurological Association to be held September 12 - 15, 2010 in San Francisco, CA.

68. Landry C, Thune R, Bastian FO. SMCA adhesin: recombinant protein in E. coli. 18th Congress of the International organization of Mycoplasmology, Chianciano Terme, Italy July 11- 16, 2010

69. Landry C, Bastian FO, Thune R. Complete gene sequence of SMCA strain of S. mirum. 18th Congress of the International organization of Mycoplasmology, Chianciano Terme, Italy July 11- 16, 2010

70. Bastian et al., ASM meeting NO 2011

71. Bastian FO. Isolation of novel Spiroplasma sp. from TSE tissues. IOM meeting, Portsmouth, New Hampshire, July 9-12, 2018.