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TESIS DOCTORAL Estudio Metagenómico De La Comunidad De TESIS DOCTORAL Estudio metagenómico de la comunidad de virus y de su interacción con la microbiota en la cavidad bucal humana Marcos Parras Moltó Madrid, 2019 Estudio metagenómico de la comunidad de virus y de su interacción con la microbiota en la cavidad bucal humana Memoria presentada por Marcos Parras Moltó para optar al título de Doctor por la Universidad Autónoma de Madrid Esta Tesis se ha realizado en el Centro de Biología Molecular Severo Ochoa bajo la supervisión del Tutor y Director Alberto López Bueno, en el Programa de Doctorado en Biociencias Moleculares (RD 99/2011) Universidad Autónoma de Madrid Facultad de Ciencias Departamento de Biología Molecular Centro de Biología Molecular Severo Ochoa (CBMSO) Madrid, 2019 El Dr. Alberto López Bueno, Profesor Contratado Doctor en el Departamento de Biología Molecular de la Universidad Autónoma de Madrid (UAM) e investigador en el Centro de Biología Molecular Severo Ochoa (CBMSO): CERTIFICA: Haber dirigido y supervisado la Tesis Doctoral titulada "Estudio metagenómico de la comunidad de virus y de su interacción con la microbiota en la cavidad bucal humana” realizada por D. Marcos Parras Moltó, en el Programa de Doctorado en Biociencias Moleculares de la Universidad Autónoma de Madrid, por lo que autoriza la presentación de la misma. Madrid, a 23 de Abril de 2019, Alberto López Bueno La presente tesis doctoral ha sido posible gracias a la concesión de una “Ayuda para Contratos Predoctorales para la Formación de Doctores” convocatoria de 2013 (BES-2013-064773) asociada al proyecto SAF2012-38421 del Ministerio de Economía y Competitividad. Durante esta tesis se realizó una estancia de dos meses en el laboratorio del Catedrático Francisco Rodríguez Valera, director de grupo de investigación: Evolutionary Genomics Group de la Universidad Miguel Hernández de Elche (San Juan de Alicante), gracias a una “Ayuda a la Movilidad Predoctoral para la Realización de Estancias Breves en Centros de I+D” convocatoria de 2015 (EEBB-I-16-11876) concedida por el Ministerio de Economía y Competitividad. AGRADECIMIENTOS AGRADECIMIENTOS Quisiera agradecer en primer lugar a Alberto López Bueno el haberme dado la oportunidad de desarrollar esta tesis bajo su tutela y dirección. Recuerdo como si fuera ayer aquella entrevista por Skype a pocos días de finalizar el plazo de entrega de documentación para la FPI, como si fuera ayer el día en que eché a volar. Gracias por tu paciencia (esto quisiera resaltarlo especialmente), compromiso y entrega. Gracias por ser tan buen profesional tanto como docente como científico, por demostrarme que quien quiere puede, que con esfuerzo se consiguen las cosas y que los límites no provienen de uno mismo. Más que un jefe, un amigo y compañero. Aquel Viernes llamando por primera vez a Paco, rellenando la documentación necesaria para la estancia antes de las 15.00, hora en que se cumplía el deadline...grandes momentos que meteré por siempre en la nube de mi memoria. Mención especial a Patricia y Ana, por haber sido mi motor al principio, y es que este grupo no siempre fue cosa solo de dos. Agradecer al 203 y al 224 su acogida. Gracias a Rocío por ser un par de manos para un manco de las pipetas, gracias a ti hoy presumo de “saber” hacer mini y maxipreps, poca broma para un bioinformático. A Mari Carmen por su alegría y su atención, a Carolina y Leyre por ayudarme siempre que han podido, a Bruno por aportar cada día ese punto friki que alegra las mañanas, a Dani por enseñarme tan bien lo que es ser un machanguito y Antonio por darme cabida bajo su techo y haberme permitido así conocer a tan maravillosa familia. El killo siempre estará para esas cañas de los Viernes a la una. A mis compañeros y amigos predocs así como al postdoc que siempre nos ha cuidado. A Rastrojo, por su sabiduría, paciencia, por haber sido mi segundo maestro en esta aventura. Una auténtica máquina. A Domingo, porque tu sistema de puntos me lo llevaré donde vaya. Murcia está orgullosa de haber sido cuna de semejante esperpento de la verborrea hispanohablante. Que nunca falte paparajote a pajera abierta. A Carlos, por ser mi parigual videojugabilistico. Y es que aunque haya sido una pena que llegases a mitad de la partida, aquí estamos ya, frente al jefe final. A Graciela, por haberme llevado a los rincones más maravillosos de la geografía española. Ya no me imagino la vida sin una visita anual a Asturias. Gracias por evitarme ese último culín aquel día, en aquella cena, en aquel lugar. Gracias a Pepa por su desparpajo y su alegría desde primera hora de la mañana. Vaya fiesta montabamos cada vez que traías comida para compartir. A cada uno de los predocs del 224: Nooshin, Carlos, Rebeca, Tania, y todas aquellas personas que eventualmente han residido bajo el techo de nuestro actual hogar. Gracias Pepe por prestarnos ese hueco en el que hemos podido desarrollar nuestras locuras científicas. Y no me olvido de la “locura” de cada tarde. Gracias Rosa por darnos una última chispa de energía cada día cuando ya no podíamos más, tu alegría es contagiosa. En resumen, gracias a todas las personas que he tenido la suerte de conocer en el CBM, como Silvia, pero en especial a ese vato que me llenó de ilusión e historias desde el primer día. Gracias Callejas por apoyarme, ayudarme, enseñarme y preocuparte por mi en todo momento. Echaré de menos esas charlas durante la comida y las subsiguientes rondas de preguntas aleatorias. No, los españoles no solemos hacer armaduras en nuestros ratos libres. No es casualidad que lo tuyo sea la numismática, porque vales oro. Gracias a Francisco Rodríguez Varela por abrirme las puertas de su laboratorio y permitirme aprender de su mano. A cada una de las personas que compartieron conmigo esos dos meses: Rafa, Ricardo, Rohit, José y especialmente a Mario. Gracias por tu dedicación y por tu alegría. No hubo mejor manera de conocer Alicante. Gracias a Alex Mira y Áurea, a Asier y José Manuel, por su colaboración en este proyecto. De la misma forma, agradecer al servicio de secuenciación del parque científico de Madrid y a los integrantes del grupo de bioinformática del CBM, especialmente a Alfonso, su labor estos años. Gracias a Antoñito por ser mi familia en Madrid, un amigo para lo bueno y para lo malo. Que no nos quede un festival de electrónica por pisar. A Borja y Sandra, por ser ese trozo de Madrid que llevaré por siempre en la mochila allá donde vaya. Agradecer a todos lo que han formado parte de mi mundo durante esos años de viajes a La Terreta. He tenido la suerte de compartir tiempo y vivencias con personas maravillosas que han crecido bajo el techo del IATA, demostrando que la mejor ciencia es la que se hace desde un grupo de amigos tan grande, dentro y fuera del laboratorio. A Fani por enseñarme, ayudarme, escucharme y abrirme un nuevo mundo de oportunidades. Nunca dejes de luchar por tus sueños. Gracias por confiar en mí. A Walter por su alegría y por ser un profesional de los pies a la cabeza. Aún tengo ganas de uno de esos cursos de bautismo (de buceo, no nos echemos las manos a la cabeza). A Alba por ser siempre una persona tan maravillosa. Necesito que me expliques el secreto para mantener siempre una sonrisa en la cara, llueve o truene. Y en definitiva al resto de la secta, la que yo conocí: Adri, Anto, Aurora, Ceci, Lucía, Ric y Sara. Cada uno me habéis aportado para ayudarme a ser lo que soy hoy. Bien saben cada una de las horchatas, paellas y buñuelos, cada grano de arena de la playa y mililitro de crema solar, que luché por entrar en la secta, pero nada oye, no hubo manera. Esa manera de ser felices y ese buen rollo, patentadlo. La familia que se elige, qué suerte tuve con la mía. Casas rurales, cumpleaños, quedadas en cualquier ciudad o país. La suerte de generar una amistad como esta, imperturbable por el paso del tiempo o las condiciones personales de cada uno, es oro. A Mer, Barbi, Marguy, CdC, CrisR, María, Angela, Anabel, Álvaro, Marta, La negra y a cada uno de vuestros +1. A mis niños, osú que cuatro. Curro, Carlos, Javi y Paco, ¡Ay, mi Parco!. No sería de justicia poner solo una frase relacionada con AGRADECIMIENTOS cada uno, debería escribir un libro entero en cada caso. Sin vosotros yo sería la mitad, quizá ni un cuarto. Coatí hasta la tumba. Y a esa familia, la que fue mi primera familia cuando yo vivía aún asustado dentro de mi cascarón. Gracias Vane, Natalia y Anita por ser mis amigas entonces y ahora, por enseñarme que la locura es un estado más de la cordura y que sonriendo se vive más feliz. Y este viaje termina donde empezó, en Sevilla, mi remanso de paz. A los que siempre estuvieron, están y estarán, defendiendo Sevilla Este. A Ignacio y Sandra por tan buenas charlas enfrentando nuestros puntos de vista sobre la vida, acompañadas siempre por un juego de mesa o un mando. A Tocho, por ayudarme a ahogar mis neuronas siempre que lo he necesitado, dentro de cualquiera de los significados que esa expresión pueda adquirir. A Juan Carlos por haber estado siempre ahí, por motivarme con su ilusión. A Andrés por acompañarme en las noches de vigilia frente a la pantalla. A Ale por acompañarme en esos paseítos de despeje diarios. A Vilches por haber recorrido junto a mí este camino durante tanto tiempo. Amigos. Que nunca nos falte un juego de mesa, una película, una partida a algún videojuego aleatorio, una noche hasta las tantas.
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