NUSANTARA BIOSCIENCE ISSN: 2087-3948 Vol. 12, No. 2, pp. 91-96 E-ISSN: 2087-3956 November 2020 DOI: 10.13057/nusbiosci/n120202 Short Communication: Lugol’s iodine test on Rafflesia patma–Tetrastigma leucostaphylum intersection tissue for preliminary starch visualization ADHITYO WICAKSONO1,♥, SOFI MURSIDAWATI2,♥♥ 1Division of Biotechnology, Generasi Biologi Indonesia Foundation. Jl. Swadaya Barat no. 4, Gresik 61171, East Java, Indonesia. ♥email:
[email protected];
[email protected] 2Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences. Jl. Ir. H. Juanda No.13, Bogor 16022, West Java, Indonesia. Tel.: +62-251-8311362, 8336871, ♥♥email:
[email protected] Manuscript received: 19 July 2020. Revision accepted: 26 August 2020. Abstract. Wicaksono A, Mursidawati S. 2020. Short Communication: Lugol’s iodine test on Rafflesia patma-Tetrastigma leucostaphylum intersection tissue for preliminary starch visualization. Nusantara Bioscience 12: 91-96. As holoparasitic plant, Rafflesia has no recognizable plastid genome, but it has plastid-like organelle. Despite the fact that it obtains nutrients from host plant, it is unknown if Rafflesia stores primary metabolites, such as carbohydrates, from its host. A study was performed to visualize the starch in Rafflesia patma Blume proximal tissue which was intersected to its host root, Tetrastigma leucostaphylum (Dennst.) Alston, using modified Sachs’ test with Lugol’s iodine. The result revealed the absence of blackening in the R. patma tissue caused by starch reaction with the iodine, but occurred in the root cortical tissue of T. leucostaphylum. The absence of starch in R. patma tissue indicated that possibly the plastid-like organ has no similar function to amyloplast, and starch is not used for storage in the flower.