Supplementary Table S5. Ingenuity Curated Microrna Target Genes

microRNA target Genes KEGG Pathway (Ingenuity curated list) hsa05221 Acute Myeloid Leukemia Signaling MAP2K4,RUNX1,MYC,MTOR,NRAS,PIK3R1,HRAS, KRAS,STAT3,CCND1 hsa05219 Bladder Cancer Signaling TP53,FGF16,IL8,NRAS,THBS1,HRAS,MMP13, KRAS,CCND1,MYC,VEGFA,FGFR3,RB1,CDKN1A,E2F1 ,CHD1,RPS6KA5 hsa05220 Chronic Myeloid Leukemia Signaling TP53, RBL2, TGFBR1,NRAS,PIK3R1,SMAD3, CDK6,HRAS,KRAS,E2F3,CCND1,MYC,TGFBR2, RB1,BCL2L1,CDKN1A,E2F1,SMAD4,E2F2 hsa05210 Colorectal Cancer Metastasis Signaling MAP2K4,TGFBR1,JAK1,SMAD3,PIK3R1,HRAS,MMP13, KRAS,CCND1,VEGFA,TGFBR2,MYC,JUN, RHOG,RHOB,SMAD4,TP53 (includes EG:22059),NRAS,CASP3,IL6R,STAT3,MAPK12,BCL2L 1,TLR4,PTGS2,WNT1,WNT5A hsa05213 Endometrial Cancer Signaling TP53 , MYC,NRAS,PIK3R1,HRAS,KRAS,CCND1,PTEN

hsa04510 FAK Signaling NRAS,PIK3R1,ACTA2,ITGA2,ITGA5,HRAS,PAK7, KRAS,PTEN hsa05214 Glioma Signaling TP53 (includes EG:22059),RBL2 (includes EG:100331892),NRAS,PIK3R1,CDK6,HRAS,KRAS,E2F 3,CCND1,PTEN,RB1,MTOR,CDKN1A,E2F1,IGF1R,E2F 2 hsa04910 ILK Signaling MAP2K4,CASP3,PIK3R1,ACTA2,VIM,MAPK12,CCND1,I TGB3,PTEN,MYC,VEGFA,MTOR,RHOG,JUN,RHOB,PP P2R4,CFL2,CHD1,RPS6KA5,PTGS2,DSP hsa04010 ERK/MAPK Signaling PPARG,NRAS,PIK3R1,ITGA2,ITGA5,HRAS,KRAS,STAT 3,MYC,PPP1R7,PPP2R4,PAK7,RPS6KA5,ESR1 hsa05218 Insulin Receptor Signaling MTOR,JAK1,GYS1,NRAS,FOXO1 (includes EG:2308),PPP1R7,PIK3R1,RPTOR,HRAS,KRAS,PTEN

hsa04150 Melanoma Signaling TP53 (includes EG:22059),RB1,NRAS,PIK3R1,E2F1,CDKN1A, CHD1,HRAS,KRAS,CCND1,PTEN hsa00190 mTOR Signaling NRAS,PIK3R1,EIF3J,HRAS,KRAS,VEGFA,HMOX1,MTO R,RHOG,RHOB,EIF4G2,PPP2R4,RPTOR, RPS6KA5

hsa04115 p53 Signaling TP53 (includes EG:22059),TP63,THBS1,PIK3R1,TNFRSF10B,CSNK1 D,APAF1,CCND1,FAS,PTEN,BCL2,RB1,BCL2L1,JUN, CDKN1A,E2F1,SERPINB5 hsa05212 Pancreatic Adenocarcinoma Signaling TP53 (includes EG:22059),MAP2K4,TGFBR1,JAK1,SMAD3,PIK3R1,KR AS,STAT3,E2F3,MAPK12,CCND1,BCL2,VEGFA,TGFBR 2,BCL2L1,HMOX1,RB1,CDKN1A,E2F1,SMAD4,PTGS2, NOTCH1,E2F2 hsa05215 Prostate Cancer Signaling TP53 (includes EG:22059),NRAS,PIK3R1,HRAS,KRAS,CCND1,PTEN, BCL2,RB1,MTOR,FOXO1 (includes EG:2308),E2F1,CDKN1A hsa05211 Renal Cell Carcinoma Signaling VEGFA,MET,JUN,NRAS,PIK3R1,HRAS,PAK7,KRAS hsa04660 T Cell Receptor Signaling MAP2K4,JUN,NRAS,PIK3R1,HRAS,KRAS,PPP3CA hsa04350 TGF-β Signaling MAP2K4,NRAS,TGFBR1,SMAD3,ACVR1,HRAS,BMPR2, KRAS,SMAD5,MAPK12,BCL2,TGFBR2,JUN,RUNX2,SM AD4 hsa04930 Type II Diabetes Mellitus Signaling MAP2K4,PPARG,MTOR,PIK3R1,MAPK12,SOCS5 hsa04370 VEGF Signaling VEGFA,BCL2L1,NRAS,FOXO1 (includes EG:2308),PIK3R1,ACTA2,HRAS,KRAS,BCL2 hsa04310 Wnt/β-catenin Signaling TP53 (includes EG:22059),TGFBR1,CSNK1D,ACVR1,CCND1,TGFBR2 ,MYC,JUN,PPP2R4,BTRC,WNT1,SOX5,WNT5A

Supplementary Table S5. Ingenuity curated microRNA target genes. Gene target components of KEGG pathways as determined with Ingenuity miRPATH microRNA Target Filter analysis of experimentally validated microRNA–mRNA target interactions. Genes include those involved in signaling pathways, which regulate neural crest maturation (e.g. BMP, Wnt, Notch and SMAD signaling).