Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing Sol A Jeon1,2, Jong Lyul Park3, Jong-Hwan Kim1, Jeong Hwan Kim1, Research 1,2 4,5 1,3* communication Yong Sung Kim , Jin Cheon Kim , Seon-Young Kim 1Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience eISSN 2234-0742 and Biotechnology, Daejeon 34141, Korea 2 Genomics Inform 2019;17(3):e32 Department of Bioscience, University of Science and Technology, Daejeon 34113, Korea 3 https://doi.org/10.5808/GI.2019.17.3.e32 Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea 4Department of Surgery, University of Ulsan College of Medicine, Seoul, Korea 5Department of Cancer Research, Institute of Innovative Cancer Research and Asan Received: August 19, 2019 Institute for Life Sciences, Asan Medical Center, Seoul 05505, Korea Revised: September 20, 2019 Accepted: September 20, 2019 Currently, Illumina sequencers are the globally leading sequencing platform in the *Corresponding author: next-generation sequencing market. Recently, MGI Tech launched a series of new sequenc- E-mail:
[email protected] ers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data faster and at lower prices than Illumina’s sequencers. In this study, we compared the per- formance of two major sequencers (MGISEQ-2000 and HiSeq 4000) to test whether the MGISEQ-2000 sequencer delivers high-quality sequence data as suggested. We performed RNA sequencing of four human colon cancer samples with the two platforms, and com- pared the sequencing quality and expression values. The data produced from the MGIS- EQ-2000 and HiSeq 4000 showed high concordance, with Pearson correlation coefficients ranging from 0.98 to 0.99.