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Quantikine® ELISA Quantikine® ELISA Human ADAMTS13 Immunoassay Catalog Number DADT130 For the quantitative determination of human A Disintegrin And Metalloproteinase with Thombospondin type 1 motif, 13 (ADAMTS13) concentrations in cell culture supernates, serum, and plasma. This package insert must be read in its entirety before using this product. For research use only. Not for use in diagnostic procedures. TABLE OF CONTENTS SECTION PAGE INTRODUCTION .....................................................................................................................................................................1 PRINCIPLE OF THE ASSAY ...................................................................................................................................................2 LIMITATIONS OF THE PROCEDURE .................................................................................................................................2 TECHNICAL HINTS .................................................................................................................................................................2 MATERIALS PROVIDED & STORAGE CONDITIONS ...................................................................................................3 OTHER SUPPLIES REQUIRED .............................................................................................................................................4 PRECAUTIONS .........................................................................................................................................................................4 SAMPLE COLLECTION & STORAGE .................................................................................................................................4 SAMPLE PREPARATION........................................................................................................................................................4 REAGENT PREPARATION .....................................................................................................................................................5 ASSAY PROCEDURE .............................................................................................................................................................6 CALCULATION OF RESULTS ...............................................................................................................................................7 TYPICAL DATA .........................................................................................................................................................................7 PRECISION ................................................................................................................................................................................8 RECOVERY.................................................................................................................................................................................8 LINEARITY .................................................................................................................................................................................9 SENSITIVITY .............................................................................................................................................................................9 CALIBRATION ..........................................................................................................................................................................9 SAMPLE VALUES .....................................................................................................................................................................9 SPECIFICITY ........................................................................................................................................................................... 10 REFERENCES ......................................................................................................................................................................... 10 Manufactured and Distributed by: USA R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413 TEL: 800 343 7475 612 379 2956 FAX: 612 656 4400 E-MAIL: [email protected] Distributed by: Europe | Middle East | Africa Bio-Techne Ltd. China Bio-Techne China Co., Ltd. 19 Barton Lane, Abingdon Science Park Unit 1901, Tower 3, Raffles City Changning Office, Abingdon OX14 3NB, UK 1193 Changning Road, Shanghai PRC 200051 TEL: +44 (0)1235 529449 TEL: +86 (21) 52380373 (400) 821-3475 FAX: +44 (0)1235 533420 FAX: +86 (21) 52371001 E-MAIL: [email protected] E-MAIL: [email protected] INTRODUCTION ADAMTS13 (A Disintegrin And Metalloproteinase with Thrombospondin type 1 motif, 13), also known as von Willebrand factor (vWF)-cleaving protease, belongs to the ADAMTS superfamily of secreted multidomain zinc metalloproteases. Full-length human ADAMTS13 contains 1427 amino acid residues that are organized into the following domains: signal peptide, propeptide, catalytic, disintegrin-like, thrombospondin type 1 (TSP1), cysteine rich, spacer, seven TSP1 repeats, and two CUB domains. The propeptide contains a furin cleavage consensus motif and is most likely removed in the Golgi or at the cell surface, releasing the 190 kDa active enzyme (1-4). ADAMTS13 is mainly produced by the liver and circulates in the blood (5). vWF, the only known substrate for ADAMTS13, is a large multimeric glycoprotein that is required for platelet adhesion and thrombus formation (1, 2, 6). The hemostatic potential of vWF increases with its length. ADAMTS13 cleaves the ultra-large multimeric vWF (ULvWF) within the A2 domain between Tyr1605 and Met1606, generating smaller, less adhesive multimers (1, 7). Shear stress is required to induce a conformational change in the ULvWF fibers to expose the cleavage site (8). In addition to its role in platelet adhesion, ULvWF has been shown to promote leukocyte adhesion and extravasation during inflammation (9). By cleaving ULvWF, ADAMTS13 down-regulates not only thrombosis but also inflammation. As a result, decreased ADAMTS13 activity accelerates inflammatory diseases and is associated with acute and chronic inflammation (9, 10). ADAMTS13 deficiency as a result of hereditary gene mutations or inhibitory autoantibodies to ADAMTS13 is a cause of thrombotic thrombocytopenic purpura (TTP), which is a rare condition of the blood-coagulation system. TTP is characterized by extensive formation of microthrombi in the microvasculature and may be accompanied by neurological dysfunction and renal failure (11-15). Additional pathologies associated with decreased circulating ADAMTS13 include liver cirrhosis, sepsis, alcoholic hepatitis, and acute pancreatitis (16-19). Low plasma ADAMTS13 is a useful predictor of cardiac and cerebrovascular events in coronary artery diseases (20, 21). After acute myocardial infarction, early decrease of ADAMTS13 levels is a predictor of future thrombotic events (22). In atrial fibrillation (AF), ADAMTS13 level in patients after treatment with cardioconversion is useful for prediction of recurrence of AF (23). The Quantikine® Human ADAMTS13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ADAMTS13 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human ADAMTS13 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ADAMTS13 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ADAMTS13. www.RnDSystems.com 1 PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human ADAMTS13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS13 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human ADAMTS13 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS13 bound in the initial step. The color development is stopped and the intensity of the color is measured. LIMITATIONS OF THE PROCEDURE • FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. • The kit should not be used beyond the expiration date on the kit label. • Do not mix or substitute reagents with those from other lots or sources. • If samples generate values higher than the highest standard, further dilute the samples with calibrator diluent and repeat the assay. • Any variation in diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding. • This assay is designed to eliminate interference by other factors present in biological samples. Until these factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. TECHNICAL HINTS • When mixing or reconstituting protein solutions, always avoid foaming. • To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. • To ensure accurate results, proper adhesion of plate sealers during incubation
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