Inflammatory Oedema Induced by Lachesis Muta Muta
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Record of the Occurrence of Lachesis Muta (Serpentes, Viperidae) in an Atlantic Forest Fragment in Paraíba, Brazil, with Comments on the Species’ Preservation Status
Biotemas, 26 (2): 283-286, junho de 2013 doi: 10.5007/2175-7925.2013v26n2p283283 ISSNe 2175-7925 Short Communication Record of the occurrence of Lachesis muta (Serpentes, Viperidae) in an Atlantic Forest fragment in Paraíba, Brazil, with comments on the species’ preservation status Ricardo Rodrigues 1* Ralph Lacerda de Albuquerque 1 Diego José Santana 1 Daniel Orsi Laranjeiras 1 Arielson Santos Protázio 1 Frederico Gustavo Rodrigues França 2 Daniel Oliveira Mesquita 1 1 Universidade Federal da Paraíba, Centro de Ciências Exatas e da Natureza Departamento de Sistemática e Ecologia, Laboratório de Herpetologia, Cidade Universitária CEP 58059-900, João Pessoa – PB, Brazil 2 Universidade Federal da Paraíba, Centro de Ciências Aplicadas e Educação, Campus IV, Litoral Norte Rua da Mangueira, s/n, CEP 58297-000, Rio Tinto – PB, Brazil *Corresponding author [email protected] Submetido em 16/07/2012 Aceito para publicação em 19/01/2013 Resumo Registro de ocorrência de Lachesis muta (Serpentes, Viperidae) em um fragmento de Mata Atlântica na Paraíba, Brasil, com comentários sobre o status de preservação da espécie. Neste estudo é descrito um novo registro da serpente pico-de-jaca, Lachesis muta, em um fragmento de Mata Atlântica no estado da Paraíba, Nordeste do Brasil. Essa espécie é considerada a maior das serpentes peçonhentas do Novo Mundo. O espécime foi encontrado durante a noite, cruzando um atalho estreito, próximo a um declive, a aproximadamente 20m de uma queda d’água. A ocorrência de L. muta nesse fragmento demonstra a importância da conservação dos fragmentos de Mata Atlântica para a preservação dessa espécie. Palavras-chave: Conservação; Lachesis muta; Mata Atlântica; Surucucu-pico-de-jaca; Viperidae Abstract In this study, one describes a new record of the bushmaster snake, Lachesis muta, in an Atlantic Forest fragment in the state of Paraíba, northeastern Brazil. -
Characterization of a Hemorrhage-Inducing Component Present in Bitis Arietans Venom
Vol. 14(12), pp. 999-1008, 25 March, 2015 DOI: 10.5897/AJB2014.14319 Article Number: AF0A87151680 ISSN 1684-5315 African Journal of Biotechnology Copyright © 2015 Author(s) retain the copyright of this article http://www.academicjournals.org/AJB Full Length Research Paper Characterization of a hemorrhage-inducing component present in Bitis arietans venom Tahís Louvain de Souza1, Fábio C. Magnoli2 and Wilmar Dias da Silva2* 1Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy ribeiro, Campos dos Goytacazes, RJ, Brazil. 2Laboratório de Imunoquímica, Instituto Butantan, São Paulo, SP , Brazil. Received 13 November, 2014; Accepted 16 February, 2015 Better characterization of individual snake venom toxins is useful for analyzing the association of their toxic domains and relevant antigenic epitopes. Here we analyzed the Bitis arietans hemorrhagic- inducing toxin present in a representative venom sample. Among the 1´ to 5´ protein peaks isolated using a Sephacryl S 100 HR chromatography column, hemorrhagic activity was expressed by all according to the following intensity, P´5 > P´3 > P´2 >, P´4. The proteins were recognized and measured with antibodies present in polyvalent horse F(ab)´2 anti-B. arietans, Bitis spp., Lachesis muta, B. atrox, Bothrops spp., Crotalus spp., and Naja spp. or in IgY anti-B. arietans and anti-Bitis spp. using enzyme- linked immunosorbent assays and western blotting. In addition, in an in vitro-in vivo assay these anti- venoms were able to block hemorrhagic-inducing activity. The evident cross-reactivity expressed by different specific anti-venoms indicates that metalloproteinases induce an immunological signature indicating the presence of similar antigenic epitopes for several snake venoms. -
Preclinical Testing of Peruvian Anti-Bothropic Anti-Venom Against Bothrops Andianus Snake Venom
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Toxicon 60 (2012) 1018–1021 Contents lists available at SciVerse ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon Short communication Preclinical testing of Peruvian anti-bothropic anti-venom against Bothrops andianus snake venom Francisco S. Schneider a, Maria C. Starling a, Clara G. Duarte a, Ricardo Machado de Avila a, Evanguedes Kalapothakis a, Walter Silva Suarez b, Benigno Tintaya b, Karin Flores Garrido b, Silvia Seraylan Ormachea b, Armando Yarleque c, César Bonilla b, Carlos Chávez-Olórtegui a,* a Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, CP: 486, CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil b Instituto Nacional de Salud, Lima, Peru c Universidad Nacional Mayor de San Marcos, Lima, Peru article info abstract Article history: Bothrops andianus is a venomous snake found in the area of Machu Picchu (Peru). Its Received 3 April 2012 venom is not included in the antigenic pool used for production of the Peruvian anti- Received in revised form 20 June 2012 bothropic anti-venom. B. andianus venom can elicit many biological effects such as Accepted 28 June 2012 hemorrhage, hemolysis, proteolytic activity and lethality. The Peruvian anti-bothropic Available online 14 July 2012 anti-venom displays consistent cross-reactivity with B. andianus venom, by ELISA and Western Blotting and is also effective in neutralizing the venom’s toxic activities. Keywords: Ó 2012 Elsevier Ltd. Open access under the Elsevier OA license. -
Snake Venom from the Venezuelan C
BOLETÍN DE MALARIOLOGÍA Y SALUD AMBIENTAL Agosto-Diciembre 2014, Vol. LIV (2): 138-149 Biochemical and biological characterisation of lancehead (Bothrops venezuelensis Sandner 1952) snake venom from the Venezuelan Central Coastal range Caracterización bioquímica y biológica del veneno de la serpiente "tigra mariposa" (Bothrops venezuelensis Sandner 1952) de la región central de la Cordillera de la Costa Venezolana Elda E. Sánchez1, María E. Girón2, Nestor L. Uzcátegui2, Belsy Guerrero3, Max Saucedo1, Esteban Cuevas1 & Alexis Rodríguez-Acosta2* RESUMEN SUMMARY Se aislaron fracciones del veneno de Bothrops Venom fractions isolated from Bothrops venezuelensis que demuestran ser un espectro abundante de venezuelensis were shown to contain a broad spectrum proteínas con actividades variadas (coagulante, hemorrágica, of proteins with varied activities. This study describes fibrinolítica, proteolítica y de función plaquetaria), para el venom fractions with coagulant, haemorrhagic, fibrinolytic, análisis de sus propiedades físico-químicas y biológicas, proteolytic and antiplatelet activities, and analyses their el veneno fue fraccionado por cromatografía de exclusión physico-chemical properties and biological activities via molecular, corrido en una electroforesis en gel y realizada molecular exclusion chromatography, gel electrophoresis una batería de ensayos biológicos. La DL50 del veneno and a bioassay battery. The LD50, determined by injecting de B. venezuelensis fue 6,39 mg/kg de peso corporal, fue intraperitoneally serial dilutions of B. venezuelensis determinada inyectando intraperitonealmente en ratones, venom into mice, was 6.39 mg/kg body weight. Twelve diluciones seriadas de veneno de B. venezuelensis. Se fractions were collected from B. venezuelensis venom colectaron doce fracciones a partir del veneno de B. using molecular exclusion chromatography. Of these, venezuelensis mediante cromatografía de exclusión molecular. -
Hunting of Herpetofauna in Montane, Coastal and Dryland Areas Of
Herpetological Conservation and Biology 8(3):652−666. HSuebrpmeittotelodg: i1c6a lM Caoyn s2e0r1v3at;i Aonc caenpdt eBdi:o 2lo5g Oy ctober 2013; Published: 31 December 2013. Hunting of Herpetofauna in Montane , C oastal , and dryland areas of nortHeastern Brazil . Hugo Fernandes -F erreira 1,3 , s anjay Veiga Mendonça 2, r ono LiMa Cruz 3, d iVa Maria Borges -n ojosa 3, and rôMuLo roMeu nóBrega aLVes 4 1Universidade Federal da Paraíba, Departamento de Sistemática e Ecologia, Postal Code 58051-900, João Pessoa, Paraíba, Brazil, e-mail: [email protected] 2Universidade Estadual do Ceará, Departamento de Ciências Veterinárias, Postal Code 60120-013, Fortaleza, Ceará, Brazil 3Universidade Federal do Ceará, Núcleo Regional de Ofiologia da UFC (NUROF-UFC), Departamento de Biologia, Postal Code 60455-760, Fortaleza, Ceará, Brazil 4Universidade Estadual da Paraíba, Departamento de Biologia, Postal Code 58109753, Campina Grande, Paraíba, Brazil abstract.— relationships between humans and animals have played important roles in all regions of the world and herpetofauna have important links to the cultures of many ethnic groups. Many societies around the world use these animals for a variety of purposes, such as food and medicinal use. Within this context, we examined hunting activities involving the herpetofauna in montane, dryland, and coastal areas of Ceará state, northeastern Brazil. We analyzed the diversity of species captured, how each species was used, the capture techniques employed, and the conservation implications of these activities on populations of those animals. We documented six hunting techniques and identified twenty-six species utilized (including five species threatened with extinction) belonging to 15 families as important for food (21 spp.), folk medicine (18 spp.), magic-religious purposes (1 sp.), and other uses (9 spp.). -
Caio Henrique De Oliveira Carniatto
1 CAIO HENRIQUE DE OLIVEIRA CARNIATTO Obtenção das células indiferenciadas do saco vitelino de Crotalus durissus (Linnaeus, 1758) (Ophidia: Viperidae) São Paulo 2015 2 CAIO HENRIQUE DE OLIVEIRA CARNIATTO Obtenção das células indiferenciadas do saco vitelino de Crotalus durissus (Linnaeus, 1758) (Ophidia: Viperidae) Dissertação apresentada ao Programa de Pós-Graduação em Anatomia dos Animais Domésticos e Silvestres da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo para obtenção do título de Mestre em Ciências Departamento: Cirurgia Área de concentração: Anatomia dos Animais Domésticos e Silvestres Orientador: Profa. Dra. Mariana Matera Veras São Paulo 2015 Autorizo a reprodução parcial ou total desta obra, para fins acadêmicos, desde que citada a fonte. DADOS INTERNACIONAIS DE CATALOGAÇÃO-NA-PUBLICAÇÃO (Biblioteca Virginie Buff D’Ápice da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo) T.3080 Carniatto, Caio Henrique de Oliveira FMVZ Obtenção das células indiferenciadas do saco vitelino de Crotalus durissus (Linnaeus, 1758) (Ophidia: Viperidae) / Caio Henrique de Oliveira Carniatto . -- 2015. 57 f. Dissertação (Mestrado) - Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Cirurgia , São Paulo, 2015. Programa de Pós-Graduação: Anatomia dos Animais Domésticos e Silvestres . Área de concentração: Anatomia dos Animais Domésticos e Silvestres . Orientador: Profa. Dra. Mariana Matera Veras . 1. Cascavel. 2. Crotalus. 3. Placenta. 4. Placentação. 5. Serpente. I. Título. 3 4 FOLHA DE AVALIAÇÃO Autor: CARNIATTO, Caio Henrique de Oliveira Título: Obtenção das células indiferenciadas do saco vitelino de Crotalus durissus (Linnaeus, 1758) (Ophidia: Viperidae) Dissertação apresentada ao Programa de Pós-Graduação em Anatomia dos Animais Domésticos e Silvestres da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo para obtenção do título de Mestre em Ciências Data: ____/_____/_______ Banca Examinadora Prof. -
The Search for Natural and Synthetic Inhibitors That Would Complement Antivenoms As Therapeutics for Snakebite Envenoming
toxins Review The Search for Natural and Synthetic Inhibitors That Would Complement Antivenoms as Therapeutics for Snakebite Envenoming José María Gutiérrez 1,* , Laura-Oana Albulescu 2, Rachel H. Clare 2, Nicholas R. Casewell 2 , Tarek Mohamed Abd El-Aziz 3,4 , Teresa Escalante 1 and Alexandra Rucavado 1 1 Facultad de Microbiología, Instituto Clodomiro Picado, Universidad de Costa Rica, San José 11501, Costa Rica; [email protected] (T.E.); [email protected] (A.R.) 2 Centre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK; [email protected] (L.-O.A.); [email protected] (R.H.C.); [email protected] (N.R.C.) 3 Zoology Department, Faculty of Science, Minia University, El-Minia 61519, Egypt; [email protected] 4 Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA * Correspondence: [email protected] Abstract: A global strategy, under the coordination of the World Health Organization, is being unfolded to reduce the impact of snakebite envenoming. One of the pillars of this strategy is to ensure safe and effective treatments. The mainstay in the therapy of snakebite envenoming is the administration of animal-derived antivenoms. In addition, new therapeutic options are being explored, including recombinant antibodies and natural and synthetic toxin inhibitors. In this review, snake venom toxins are classified in terms of their abundance and toxicity, and priority Citation: Gutiérrez, J.M.; Albulescu, actions are being proposed in the search for snake venom metalloproteinase (SVMP), phospholipase L.-O.; Clare, R.H.; Casewell, N.R.; A2 (PLA2), three-finger toxin (3FTx), and serine proteinase (SVSP) inhibitors. -
Souza E Me Sjrp Par.Pdf (581.4Kb)
RESSALVA Atendendo solicitação do autor, o texto completo desta dissertação será disponibilizado somente a partir de 16/04/2021. Eletra de Souza Biologia Reprodutiva da surucucu-pico-de-jaca (Lachesis muta): de Norte a Nordeste do Brasil Dissertação apresentada como parte dos requisitos para obtenção do título de Mestra em Biologia Animal, junto ao Programa de Pós-Graduação em Biologia Animal, do Instituto de Biociências, Letras e Ciências Exatas da Universidade Estadual Paulista “Júlio de Mesquita Filho”, Câmpus de São José do Rio Preto. Financiadora: CAPES Orientadora: Profª. Drª. Selma Maria de Almeida Santos São José do Rio Preto 2020 S729b Souza, Eletra de Biologia reprodutiva da surucucu-pico-de-jaca (Lachesis muta): : de Norte a Nordeste do Brasil / Eletra de Souza. -- São José do Rio Preto, 2020 142 p. : il., tabs., fotos, mapas Dissertação (mestrado) - Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto Orientadora: Selma Maria Almeida-Santos 1. Biologia. 2. Reprodução. 3. Aptidão biológica. 4. Espermatogênese em animais. 5. Lachesis muta. I. Título. Sistema de geração automática de fichas catalográficas da Unesp. Biblioteca do Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto. Dados fornecidos pelo autor(a). Essa ficha não pode ser modificada. Dedico este trabalho ao que me move a continuar perseguindo meus sonhos. Dedico à biodiversidade brasileira, nossa fauna e flora, mas também a nossa brava gente, que resiste em meio à lama, ao fogo e ao óleo. AGRADECIMENTOS O presente trabalho foi realizado com o apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Código de financiamento 001. -
Bushmaster (Lachesis Muta) Predatory Behavior at Dallas Zoo and San Diego Zoo
Bulletin of the Psychonomic Society 1989, 27 (5), 459-461 Bushmaster (Lachesis muta) predatory behavior at Dallas Zoo and San Diego Zoo DAVID CHISZAR University of ColoraOO, Boulder, ColoraOO JAMES B. MURPHY Dallas Zoo, Dallas, Texas CHARLES W. RADCLIFFE San Diego Zoo, San Diego, California and HOBART M. SMITH University of Colorado, Boulder, ColoraOO Bushmasters (Lachesis muta) that were observed during feeding episodes occasionally released rodents after the predatory strike. For one such episode, we report the presence of a sustained, high rate of tongue-flicking that lasted 136 min. In this paper, we also present photographs showing skin folds that cover the snake's pits and eyes when a rodent prey was being held following the predatory strike. We suggest that L. muta may occasionally exhibit strike-induced chemosensory searching and trail-following when large (but ingestible) prey are encountered. Adult bushmasters (Lachesis muta), which may exceed upon releasing prey in order to quantify SICS. This paper 3 m in length, typically hold rodent prey following the reports our initial observations. envenomating strike (J.B.M. and C.W.R., personal obser vations, 1986-present, while feeding bushmasters meals METHOD ranging from 1.5%-3.0% of snake body weight). These snakes have facial characteristics similar to those de Three yearling bushmasters, hatched at Dallas Zoo (August 15-18, 1987), were observed. Two were exposed to rats (60 g) held just out scribed for Porthidium nummifer by Chiszar and Radcliffe side of striking range for 3 sec (i.e., no-strike, or control, condition). (in press); the pits and eyes are partly or completely cov These prey were then removed, and all tongue flicks emitted by the snakes ered by skin folds during the period that a prey item is were recorded during the next 10 min. -
For Specific Identification of Lachesis Acrochorda Venom
The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2012 | volume 18 | issue 2 | pages 173-179 Development of a sensitive enzyme immunoassay (ELISA) for specific ER P identification of Lachesis acrochorda venom A P Núñez Rangel V (1, 2), Fernández Culma M (1), Rey-Suárez P (1), Pereañez JA (1, 3) RIGINAL O (1) Program of Ophidism and Scorpionism, University of Antioquia, Medellin, Colombia; (2) School of Microbiology, University of Antioquia, Medellin, Colombia; (3) School of Pharmaceutical Chemistry, University of Antioquia, Medellin, Colombia. Abstract: The snake genus Lachesis provokes 2 to 3% of snakebites in Colombia every year. Two Lachesis species, L. acrochorda and L. muta, share habitats with snakes from another genus, namely Bothrops asper and B. atrox. Lachesis venom causes systemic and local effects such as swelling, hemorrhaging, myonecrosis, hemostatic disorders and nephrotoxic symptoms similar to those induced by Bothrops, Portidium and Bothriechis bites. Bothrops antivenoms neutralize a variety of Lachesis venom toxins. However, these products are unable to avoid coagulation problems provoked by Lachesis snakebites. Thus, it is important to ascertain whether the envenomation was caused by a Bothrops or Lachesis snake. The present study found enzyme linked immunosorbent assay (ELISA) efficient for detecting Lachesis acrochorda venom in a concentration range of 3.9 to 1000 ng/mL, which did not show a cross-reaction with Bothrops, Portidium, Botriechis and Crotalus venoms. Furthermore, one fraction of L. acrochorda venom that did not show cross- reactivity with B. asper venom was isolated using the same ELISA antibodies; some of its proteins were identified including one Gal-specific lectin and one metalloproteinase. -
Experimental Lachesis Muta Rhombeata Envenomation and Effects of Soursop (Annona Muricata) As Natural Antivenom
Universidade de São Paulo Biblioteca Digital da Produção Intelectual - BDPI Departamento de Microbiologia - ICB/BMM Artigos e Materiais de Revistas Científicas - FCFRP/DFQ 2016 Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom Journal of Venomous Animals and Toxins including Tropical Diseases. 2016 Mar 08;22(1):12 http://www.producao.usp.br/handle/BDPI/49930 Downloaded from: Biblioteca Digital da Produção Intelectual - BDPI, Universidade de São Paulo Cremonez et al. Journal of Venomous Animals and Toxins including Tropical Diseases (2016) 22:12 DOI 10.1186/s40409-016-0067-6 RESEARCH Open Access Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom Caroline Marroni Cremonez1, Flávia Pine Leite1, Karla de Castro Figueiredo Bordon1, Felipe Augusto Cerni1, Iara Aimê Cardoso1, Zita Maria de Oliveira Gregório2, Rodrigo Cançado Gonçalves de Souza3, Ana Maria de Souza2 and Eliane Candiani Arantes1* Abstract Background: In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata. Methods: We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake venom. -
Phylogenetic Relationships Within Bothrops Neuwiedi Group
Molecular Phylogenetics and Evolution 71 (2014) 1–14 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Phylogenetic relationships within Bothrops neuwiedi group (Serpentes, Squamata): Geographically highly-structured lineages, evidence of introgressive hybridization and Neogene/Quaternary diversification ⇑ Taís Machado a, , Vinícius X. Silva b, Maria José de J. Silva a a Laboratório de Ecologia e Evolução, Instituto Butantan, Av. Dr. Vital Brazil, 1500, São Paulo, SP 05503-000, Brazil b Coleção Herpetológica Alfred Russel Wallace, Instituto de Ciências da Natureza, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700, Alfenas, MG 37130-000, Brazil article info abstract Article history: Eight current species of snakes of the Bothrops neuwiedi group are widespread in South American open Received 8 November 2012 biomes from northeastern Brazil to southeastern Argentina. In this paper, 140 samples from 93 different Revised 3 October 2013 localities were used to investigate species boundaries and to provide a hypothesis of phylogenetic rela- Accepted 5 October 2013 tionships among the members of this group based on 1122 bp of cyt b and ND4 from mitochondrial DNA Available online 17 October 2013 and also investigate the patterns and processes occurring in the evolutionary history of the group. Com- bined data recovered the B. neuwiedi group as a highly supported monophyletic group in maximum par- Keywords: simony, maximum likelihood and Bayesian analyses, as well as four major clades (Northeast I, Northeast Mitochondrial DNA II, East–West, West-South) highly-structured geographically. Monophyly was recovered only for B. pubes- Incomplete lineage sorting Hybrid zone cens.