Monoclonal Antibody to WHSC1

AM50424PU-N OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Monoclonal Antibody to WHSC1 - Purified Alternate names: KIAA1090, MMSET, Multiple myeloma SET domain-containing protein, NSD2, Nuclear SET domain-containing protein 2, Probable histone-lysine N-methyltransferase NSD2, TRX5, Trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein Catalog No.: AM50424PU-N Quantity: 0.1 mg Concentration: lot-specific Background: Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1), Nuclear SET domain containing protein 2 (NSD2) is a histone lysine methyltransferase that is deregulated in a subgroup of multiple myelomas. NSD proteins have been described to serve as oncogenes; however, little is known about their targets in transcriptional repression or activation. NSD2 is overexpressed in multiple myelomas via translocation between the NSD2 locus on chromosome 4 and the immunoglobulin locus on chromosome 14. NSD2 is under the regulatory control of this immunoglobulin locus. Further research is required to elucidate its roles and mechanisms in cancer. Uniprot ID: O96028 NCBI: NP_001035889.1 GeneID: 7468 Host / Isotype: Mouse / IgG2b Recommended SM12P, AM03110PU-N Isotype Controls: Clone: 29D1 Immunogen: GST-tagged recombinant protein corresponding to human WHSC1/NSD2. Format: State: Liquid purified Ig fraction Purification: Protein G purified. Buffer System: 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Applications: Chromatin Immunoprecipitation: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in transfected Gal4-reporter/293T cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.) Western Blot: 0.5-1 µg/mL from a representative lot detected WHSC1/NSD2 in 10 µg of HeLa nuclear extract and human placenta tissue lysate. Immunoprecipitation: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in KMS11 cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

For research and in vitro use only. Not for diagnostic or therapeutic work. MS/20150619 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 AM50424PU-N: Monoclonal Antibody to WHSC1 - Purified Specificity: Demonstrated to react with Human. Species: Human. Other species not tested. Storage: Store undiluted at 2-8°C. Shelf life: one year from despatch. Pictures: Western Blotting Analysis: Representative lot data. HCT116 cell lysate was probed with Cat. No. AM50424PU-N, Anti-WHSC1/NSD2, clone 29D1 (0.5 µg/mL ). Proteins were visualized using a Goat Anti-Mouse IgG (H+L) secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates WHSC1/NSD2 (~152 kDa Isoform 1)

Western Blotting Analysis: Representative lot data. HeLa nuclear extract (lane 1) and human placenta tissue lysate (lane 2) were probed with Cat. No. AM50424PU-N, Anti- WHSC1/NSD2, clone 29D1 (0.5 µg/mL and 1 µg/mL respectively). Proteins were visualized using a Goat Anti-Mouse IgG (H+L) secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate WHSC1/NSD2 (~81 kDa Isoform 2 and ~66 kDa Isoform 4)