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Bioorthogonal Prodrug Activation Driven by a Strain-Promoted 1,3

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Bioorthogonal Prodrug Activation Driven by a Strain-Promoted 1,3 Bioorthogonal Prodrug Activation Driven by a Strain‐Promoted 1,3‐Dipolar Cycloaddition 1 Siddarth S. Matikonda, Douglas L. Orsi, Verena Staudacher, Imogen A. Jenkins, Franziska Fiedler, Jiayi Chen and Allen B. Gamble Chemical Science, 2015, 6, pp. 1212‐ 1218 (University of Otago, Dunedin, New Zealand) A. Manos‐Turvey, Wipf Group Current Literature February 28th, 2015 Alex Manos-Turvey @ Wipf Group Page 1 of 22 3/14/2015 Prodrugs for Cancer Therapies 2 Non‐selectivity in cancer treatments leads to off‐target side‐effects Prodrug activation is seen as a viable method allowing for direct drug delivery Cleavage of a deactivating linker, leading to activation Can react with off‐target sources due to hydrolysis Antibody‐Drug Conjugates (ADCs) ADCs can elicit an immune system response The linkers need to be fine tuned between stability and “cleavability” Drugs become diluted as this is dependant on cell surface receptors, leading to < potent R.V.J. Chari, M.L. Miller, W.C. Widdison, Angew. Chem., 2014, 53, 3796‐3827 Fig: http://static.cdn‐seekingalpha.com/uploads/2014/1/19447671_13889572897936_1.jpg Alex Manos-Turvey @ Wipf Group Page 2 of 22 3/14/2015 Prodrugs for Cancer Therapies 3 Antibody‐Directed Enzyme Prodrug Therapy (ADEPT) Targets an antibody‐enzyme conjugate to a cancer cell Limited to human enzymes, to avoid anti‐enzyme immune responses K.D. Bagshawe, S.K. Sharma, R.H.J. Begent, Expert Opin. Biol. Ther., 2004, 4, 1777‐1789 K.‐C. Chen, S.‐Y. Wu, Y.‐L. Leu, Z.M. Prijovich, B.‐M. Chen, H.‐E. Wang, T.‐L. Cheng, S.R. Roffler, Bioconjugate Chem., 2011, 22, 938‐948 Alex Manos-Turvey @ Wipf Group Page 3 of 22 3/14/2015 Prodrugs for Cancer Therapies 4 Bioorthogonal Chemistry Not many examples for in vitro prodrug activation Staudinger and tetrazine‐TCO (Inverse‐Electron‐Demand Diels‐Alder Cycloadditions) reactions have been used. K. Lang, J.W. Chin, Chem. Rev., 2014, 4764‐4806 Alex Manos-Turvey @ Wipf Group Page 4 of 22 3/14/2015 Prodrugs for Cancer Therapies 5 Bioorthogonal Chemistry TCO/tetrazine prodrug activation difficulties: Lower TCO‐conjugate activity Low tumour‐to‐background ADC ratio, leading to off target effects TCO‐prodrug variant may isomerise to cis form if administered separately R.M. Versteegen, R. Rossin, W. ten Hoeve, H.M. Janssen, M.S. Robillard, Angew. Chem. Int. Ed., 2013, 52, 14112‐14116 Alex Manos-Turvey @ Wipf Group Page 5 of 22 3/14/2015 Prodrugs for Cancer Therapies: 1,3‐Dipolar Click Reaction with TCO and Azide 6 Bioorthogonal Chemistry: TCO and Azide First reported in 1992 Triazoline product is unstable K.J. Shea, J.‐S. Kim, J. Am. Chem. Soc., 1992, 4846‐485 Alex Manos-Turvey @ Wipf Group Page 6 of 22 3/14/2015 Prodrugs for Cancer Therapies: 1,3‐Dipolar Click Reaction with TCO and Azide 6 Bioorthogonal Chemistry: TCO and Azide First reported in 1992 Triazoline product is unstable K.J. Shea, J.‐S. Kim, J. Am. Chem. Soc., 1992, 4846‐485 Alex Manos-Turvey @ Wipf Group Page 7 of 22 3/14/2015 Prodrugs for Cancer Therapies: 1,3‐Dipolar Click Reaction with TCO and Azide 7 TCO and Azide for Prodrug Activation! Attach electron‐deficient linker to inactive drug TCO identifies target Aqueous environment is key Alex Manos-Turvey @ Wipf Group Page 8 of 22 3/14/2015 Proof of Concept 8 Coumarin probes were synthesised to investigate rate and mechanism of reaction A doxorubicin azido‐PABC prodrug was synthesized to investigate in vitro biorthogonal activation HO O O N3 O O O O Two TCOs were made 8a Et3N, DMF, rt O 38% O2N H2N O O i) a) NaNO2,5MHCl,H2O N OH b) NaN ,H O, 0 °C 3 3 2 O H ii) 4-NO PhCO Cl, pyridine, 2 2 O DIPEA, triphosgene, O N O O DCM, rt tol, reflux to rt O O 8b 42% 30% NH2 doxorubicin.HCl, O OH O N3 Et3N, 4A sieves, DMF, rt OH H H 69% H H O O OH O O H HO N 3 9 TCO TCO-OH OH O NH O Alex Manos-Turvey @ Wipf Group Page 9 of 22 3/14/2015 Proof of Concept 9 Tested by spectrofluorometry, measuring fluorescence (ex 360 nm, em 455 nm) in PBS:MeCN (1:1) n=3 N3 O O O O O 8a HO O O N3 H O N O O O 8b H2N O O Alex Manos-Turvey @ Wipf Group Page 10 of 22 3/14/2015 Mechanism 10 A series of 1H NMR experiments were then carried out 8a carbonate was used at 6.7 mM, TCO at 18.7 mM Alex Manos-Turvey @ Wipf Group Page 11 of 22 3/14/2015 Mechanism 11 # ! * Alex Manos-Turvey @ Wipf Group Page 12 of 22 3/14/2015 Mechanism 12 # ! $ * Alex Manos-Turvey @ Wipf Group Page 13 of 22 3/14/2015 Rate of Reaction of 1,3‐Dipolar Cycloaddition 13 TCO/TCO‐OH activation results in coumarin release RP‐HPLC was used to determine the second order rate of the initial 1,3‐dipolar cycloaddition MeCN:PBS (1:1, 37 °C), measuring disappearance of SM at 254 nm Comparable rates to those seen with first generation SPAAC (100‐10‐3 M‐1 s‐1) H H HO TCO-OH eq N N N3 N k = 0.017 M-1 s-1 O O O O 2 O O O O O HO H O 8a triazoline H HO TCO-OH ax -1 -1 k2 = 0.027 M s K.J. Shea, J.‐S. Kim, J. Am. Chem. Soc., 1992, 4846‐485 Alex Manos-Turvey @ Wipf Group Page 14 of 22 3/14/2015 Rate of Reaction for Coumarin Release 14 8b and TCO were reacted in CD3CN and DMSO‐d6 for 19 h to give 11b An aliquot of the NMR was then diluted into PBS and the rate of triazoline and imine degradation was monitored spectroscopically by the appearance of 13b Alex Manos-Turvey @ Wipf Group Page 15 of 22 3/14/2015 Rate of Reaction for Coumarin Release 15 8b and TCO were reacted in CD3CN and DMSO‐d6 for 19 h to give 11b An aliquot of the NMR was then diluted into PBS and the rate of triazoline and imine degradation was monitored spectroscopically by the appearance of 13b Alex Manos-Turvey @ Wipf Group Page 16 of 22 3/14/2015 Rate of Reaction for Coumarin Release 15 8b and TCO were reacted in CD3CN and DMSO‐d6 for 19 h to give 11b An aliquot of the NMR was then diluted into PBS and the rate of triazoline and imine degradation was monitored spectroscopically by the appearance of 13b Alex Manos-Turvey @ Wipf Group Page 17 of 22 3/14/2015 Rate of Reaction for Coumarin Release 16 N N N H O N O O O 11b - triazoline ii) dilute 1000-fold into PBS H2N O O t1/2 =19min 13b Degradation and release (3 steps) follows pseudo first‐order kinetics in polar protic solvents Assumption that either triazoline degradation or imine hydrolysis is the rate limiting step In vivo this rate is less significant as both intermediates will be fixed to a cancer cell surface Alex Manos-Turvey @ Wipf Group Page 18 of 22 3/14/2015 Bioorthogonal Potential 17 Compound IC50 (µM) H doxorubicin (15)0.71 O OH O OH H O OH O 9 pro‐drug 49.9 HO OH TCO-OH (10) 9 + CCO‐OH (100 µM) 55.0 H O O OH O O H O O OH O O N 9 + TCO‐OH (mix) (100 µM) 0.96 3 OH O NH OH 9 + TCO‐OH (eq) (100 µM) 1.47 NH2 O H2N 9 + TCO‐OH (ax) (100 µM) 1.34 9 OH 15 - doxorubicin 9 + TCO‐OH (eq) (10 µM) 4.98 7 Using a model murine melanoma cell line (B16‐OVA), the reaction strategy was evaluated in vitro following 72 h incubation with 9 and 10 at 37 °C 9 cytotoxicity alone is low The authors propose that 15 is released outside of targeted tumour cells and then diffuses into the closely located cancer cells Alex Manos-Turvey @ Wipf Group Page 19 of 22 3/14/2015 Bioorthogonal Potential 18 Attachment of 1‐6 TCOs to each monoclonal antibody with binding with 105 cell surface receptors, [TCO] = 0.4‐2.5 µM on tumour cell surfaces 9 rate of reaction still needs improvement Need to overcome rapid clearance rates from mice (t1/2 = mins) for similar compounds In vivo suitability was evaluated by monitoring stability and activation of 9 in 50% mouse serum:PBS and PBS only (HPLC analysis) Time Degradation of 9 (15 release) Degradation of 9 (15 release) + TCO‐OH eq (h) PBS only PBS + MS PBS only PBS + MS 0 100% (0%) 100% (0%) 100% (0%) 100% (0%) 4 106% (0%) 95% (0%) 84% (34%) 47% (51%) 24 121% (0%) 68% (0%) 39% (77%) 12% (59%) 48 112% (0%) 56% (6%) 12% (79%) 2% (5%) Alex Manos-Turvey @ Wipf Group Page 20 of 22 3/14/2015 Bioorthogonal Potential 19 No reaction deactivation from serum derived byproducts a problem seen with Staudinger prodrug activations Investigated if serum protein interactions may reduce effective [9] and therefore activity SPAAC cyclooctyne reactants (added after tumour targeting) interact with serum and show reduced in vivo reaction rates The reaction proceeds faster in the presence of serum than the model systems of ‐1 ‐1 8a and 8b in MeCN:PBS (k2 = 0.017‐0.027 M s ) H O OH O OH H O OH O HO OH TCO-OH (10) H O O OH O O H -1 -1 O O OH O O k2 = 0.137 M s N3 OH 50% serum:PBS O NH OH 15 - doxorubicin NH2 O 9 Alex Manos-Turvey @ Wipf Group Page 21 of 22 3/14/2015 Conclusions and Scope 22 Successfully use a 1,3‐dipolar cycloaddition between TCO‐OH and an azide, to facilitate prodrug activation Activation is 1‐2 orders of magnitude faster than the Staudinger reaction variants (and faster still in serum:PBS mixtures TCO‐OH could isomerise, but will be modified with antibody linkers known to stabilise isomerization Still room to expand on reactivity through modification of the azido‐prodrug Good stability in mouse serum (min to h) Next step: need to move from the hypothetical to the actual prodrug antibody system Alex Manos-Turvey @ Wipf Group Page 22 of 22 3/14/2015.
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