Monoclonal Anti-Albumin Antibody Produced in Mouse (A2672

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Monoclonal Anti-Albumin Antibody Produced in Mouse (A2672

MONOCLONAL ANTI-HUMAN SERUM ALBUMIN CLONE HSA-9 Mouse Ascites Fluid Product No. A 2672 Monoclonal anti-Human Serum Albumin (mouse IgG1 albumin levels may indicate disease states such as isotype) is derived from the hybridoma produced by the malnutrition, cirrhosis, nephrotic syndrome, diabetes, fusion of mouse myeloma cells and splenocytes from an gastrointestinal and hepatic diseases, thermal burns and immunized mouse. A releasate of human platelets was pulmonary disease. used as the immunogen. The isotype is determined by a double diffusion assay using immunoglobulin and Reagents subclass specific antisera. The product is provided as ascites fluid with 0.1% sodium azide as a preservative. Monoclonal anti-Human Serum Albumin is specific for human serum albumin and shows cross reactivity with Precautions rhesus monkey and baboon albumins when tested in an Due to the sodium azide content a material safety data immunoblot procedure under non-reducing conditions. sheet (MSDS) for this product has been sent to the The product detects an epitope present under attention of the safety officer of your institution. Consult non-reducing conditions. The antibody shows no cross the MSDS for information regarding hazards and safe reactivity with serum albumin from bovine, cat, catfish, handling practices. chicken, dog, donkey, gibbon, goat, guinea pig, hamster, horse, marmoset, mouse, pig, pigeon, rabbit, rat, sheep Product Profile or turkey. A minimum dilution of 1:500 was determined by ELISA using human serum albumin at 50mg/ml as the coating Monoclonal anti-Human Serum Albumin may be used for solution. the determination of albumin in human body fluids by ELISA. The antibody may be used in immunoblotting In order to obtain best results it is recommended that under non-reducing conditions. each individual user determine their optimum working dilutions by titration assay. Albumin, the major protein produced by liver cells, represents more than half of the total protein content of Storage human serum. Many other body fluids also contain For continuous use, store at 2-8 °C for up to one month. albumin. Three major functions for serum albumin have For extended storage, the solution may be frozen in been proposed: maintenance of osmotic pressure, working aliquots. Repeated freezing and thawing is not transportation of a variety of substances and an recommended. Storage in "frost-free" freezers is not endogenous source of amino acids. The primary sites of recommended. If slight turbidity occurs upon prolonged albumin degradation are not known, but the protein can storage, clarify the solution by centrifugation before use. be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used PCS 1/00 screening test in clinical medicine. A decrease in serum Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. .

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