bioRxiv preprint doi: https://doi.org/10.1101/2020.08.12.237461; this version posted August 12, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Assessment of ethanol-induced toxicity on iPSC-derived human dopaminergic neurons using a novel high-throughput mitochondrial neuronal health (MNH) assay Authors and affiliations: Annika Zink1,2,3, Josefin Conrad2, Narasimha Swami Telugu2, Sebastian Diecke2, Andreas Heinz1, Erich Wanker2, Josef Priller1,4*, Alessandro Prigione2,3* 1Department of Neuropsychiatry, Charité - Universitätsmedizin Berlin, Germany 2Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany 3Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Heinrich Heine University, Duesseldorf, Germany 4University of Edinburgh and UK DRI, Edinburgh, UK *Co-Last Authors Contact information: *Prof. Dr. Josef Priller, Department of Neuropsychiatry, Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany. Phone: +49 30 450517209, Email:
[email protected] *Prof. Dr. Alessandro Prigione, Department of General Pediatrics, Neonatology and Pediatric Cardiology, Heinrich Heine University, Duesseldorf, Germany, Phone: +49 211 81 18705, Email:
[email protected] Keywords: iPSCs, neurons, mitochondria, ethanol, high-content screening, neuronal toxicity Running title: High-throughput mitochondrial neuronal health assay 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.08.12.237461;