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The Comparative Clinical Performance of Four SARS-Cov-2 Rapid Antigen Tests and Their Correlation to Infectivity in Vitro
Journal of Clinical Medicine Article The Comparative Clinical Performance of Four SARS-CoV-2 Rapid Antigen Tests and Their Correlation to Infectivity In Vitro Niko Kohmer 1,†, Tuna Toptan 1,† , Christiane Pallas 1, Onur Karaca 1, Annika Pfeiffer 1 , Sandra Westhaus 1, Marek Widera 1 , Annemarie Berger 1, Sebastian Hoehl 1 , Martin Kammel 2,3, Sandra Ciesek 1,4,5,*,‡ and Holger F. Rabenau 1,*,‡ 1 Institute for Medical Virology, University Hospital, Goethe University Frankfurt, 60596 Frankfurt, Germany; [email protected] (N.K.); [email protected] (T.T.); [email protected] (C.P.); [email protected] (O.K.); [email protected] (A.P.); [email protected] (S.W.); [email protected] (M.W.); [email protected] (A.B.); [email protected] (S.H.) 2 Institut fuer Qualitaetssicherung in der Virusdiagnostik-IQVD der GmbH, 14129 Berlin, Germany; [email protected] 3 INSTAND Gesellschaft zur Foerderung der Qualitaetssicherung in Medizinischen Laboratorien e.V., 40223 Duesseldorf, Germany 4 German Centre for Infection Research, External Partner Site, 60323 Frankfurt, Germany 5 Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch Translational Medicine and Pharmacology, 60596 Frankfurt, Germany * Correspondence: [email protected] (S.C.); [email protected] (H.F.R.); Tel.: +49-696-301-5219 (S.C.); Tel.: +49-696-301-5312 (H.F.R.) † These authors contributed equally to this work. ‡ These authors contributed equally to this work. Abstract: Citation: Kohmer, N.; Toptan, T.; Due to globally rising numbers of severe acute respiratory syndrome coronavirus 2 (SARS- Pallas, C.; Karaca, O.; Pfeiffer, A.; CoV-2) infections, resources for real-time reverse-transcription polymerase chain reaction (rRT-PCR)- Westhaus, S.; Widera, M.; Berger, A.; based testing have been exhausted. -
Antibody-Mediated Neutralization of Authentic SARS-Cov-2 B.1.617 Variants Harboring L452R and T478K/E484Q
viruses Communication Antibody-Mediated Neutralization of Authentic SARS-CoV-2 B.1.617 Variants Harboring L452R and T478K/E484Q Alexander Wilhelm 1, Tuna Toptan 1 , Christiane Pallas 1, Timo Wolf 2, Udo Goetsch 3, Rene Gottschalk 3 , Maria J. G. T. Vehreschild 2,4,5, Sandra Ciesek 1,5,6,† and Marek Widera 1,*,† 1 Institute for Medical Virology, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, Germany; [email protected] (A.W.); [email protected] (T.T.); [email protected] (C.P.); [email protected] (S.C.) 2 Department of Internal Medicine, Infectious Diseases, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, Germany; [email protected] (T.W.); [email protected] (M.J.G.T.V.) 3 Health Protection Authority of the City of Frankfurt am Main, 60313 Frankfurt am Main, Germany; [email protected] (U.G.); [email protected] (R.G.) 4 University Center for Infectious Diseases (UCI), University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, Germany 5 German Center for Infection Research (DZIF), 38124 Braunschweig, Germany 6 Branch Translational Medicine and Pharmacology, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 60596 Frankfurt am Main, Germany * Correspondence: [email protected]; Tel.: +49-696-3018-6102; Fax: +49-69-6301-6477 † These authors equally contributed to this work. Abstract: The capacity of convalescent and vaccine-elicited sera and monoclonal antibodies (mAb) Citation: Wilhelm, A.; Toptan, T.; to neutralize SARS-CoV-2 variants is currently of high relevance to assess the protection against Pallas, C.; Wolf, T.; Goetsch, U.; infections. -
Neuberufene Professor*Innen
Neuberufene Professor*innen Prof. Dr. Sandra Ciesek Prof. Dr. Sandra Ciesek Fachbereich Medizin Professur für Medizinische Virologie Mehr zur Person in Fragen und Antworten Was war Ihre letzte Station vor der Goethe-Universität und was hat Sie nach Frankfurt gezogen? Bevor ich nach Frankfurt gekommen bin, war ich am Universitätsklinikum Essen an der Universität Duisburg- Essen als W2-Professorin für Virologie. Nach Frankfurt hat mich meine neue Aufgabe –die Leitung eines eigenen Instituts – gezogen. Was fasziniert Sie derzeit am meisten in der Forschung? Wir befinden uns derzeit in einer Pandemie, ausgelöst durch ein Virus. Mich fasziniert die Geschwindigkeit des Erkenntnisgewinns zu diesem neuen Virus SARS-CoV-2 und hier vor allem, wie Wissenschaftler*innen unterschiedlicher Disziplinen auf der ganzen Welt zusammenarbeiten mit dem gemeinsamen Ziel, diese Pandemie zu beenden. Welches Erlebnis oder welche Person hat Sie besonders geprägt? Besonders geprägt hat mich mein Doktorvater und ehemaliger Chef Prof. Dr. Michael Manns aus Hannover, bei dem ich 13 Jahre lang beschäftigt war. Ich habe von ihm sehr viel über Führungsstile, die Bedeutung von Wissenschaft und die Förderung vom Nachwuchs gelernt. Wenn die Corona-Pandemie überwunden ist, möchte ich … … erstmal eine lange Reise machen. CV Prof. Dr. med. Sandra Ciesek Work Address: Prof. Dr. med. Sandra Ciesek Institut für Medizinische Virologie Universitätsklinikum, Goethe Universität Frankfurt Paul-Ehrlich-Str. 40 60590 Frankfurt am Main Phone: +49 69 6301-5219 E-Mail [email protected] Academic education 2018 MHBA, FAU Nürnberg-Erlangen 2018 Certificate of Microbiology, Virology and Infection Epidemiology 2013 Certificate of Internal Medicine and Gastroenterology 2011 Venia legendi in Experimental Gastroenterology, Medical School Hannover, Germany 2005 MD, Medical School Hannover, Germany Mentor: Prof. -
Assessment of SARS-Cov-2 Transmission on an International Flight and Among a Tourist Group
Research Letter | Public Health Assessment of SARS-CoV-2 Transmission on an International Flight and Among a Tourist Group Sebastian Hoehl, MD; Onur Karaca; Niko Kohmer, MD; Sandra Westhaus, PhD; Jürgen Graf, MD; Udo Goetsch, MD; Sandra Ciesek, MD Introduction This case series assessed a commercial airline flight from Tel Aviv, Israel, to Frankfurt, Germany, that Author affiliations and article information are occurred on March 9th, 2020. Among 102 passengers on a Boeing 737-900 aircraft were 24 listed at the end of this article. members of a tourist group. Starting 7 days earlier, the group had contact with a hotel manager who later received a diagnosis of coronavirus disease 2019 (COVID-19). No member of the group had received a diagnosis of COVID-19 before the flight, and no measures to prevent transmission (eg, wearing of masks) had been applied. The flight duration was 4 hours 40 minutes. Methods At the destination airport, we conducted a medical evaluation of the tourist group, including testing for severe acute respiratory syndrome coronavirus (SARS-CoV-2) in a throat swab specimen. In addition, we contacted all passengers 4 to 5 weeks later by phone and conducted structured interviews. Passengers were asked whether they had contact with a person with COVID-19. They were prompted to report symptoms and asked about previous testing for SARS-CoV-2. A semiquantitative SARS-CoV-2 IgG antibody test (EUROIMMUN) was offered to all passengers who had been seated within 2 rows of the index cases and to those who reported to have been symptomatic. Borderline and positive results in the IgG test were confirmed with a plaque reduction neutralization test (PRNT). -
The Green Tea Polyphenol, Epigallocatechin3gallate, Inhibits Hepatitis C Virus Entry
The Green Tea Polyphenol, Epigallocatechin-3-Gallate, Inhibits Hepatitis C Virus Entry Sandra Ciesek,1,2 Thomas von Hahn,1 Che C. Colpitts,3 Luis M. Schang,3 Martina Friesland,2 Jo¨rg Steinmann,4 Michael P. Manns,1 Michael Ott,1 Heiner Wedemeyer,1 Philip Meuleman,5 Thomas Pietschmann,2 and Eike Steinmann2 Hepatitis C virus (HCV) is a major cause of liver cirrhosis and hepatocellular carcinoma. Current antiviral therapy fails to clear infection in a substantial proportion of cases. Drug development is focused on nonstructural proteins required for RNA replication. Individu- als undergoing orthotopic liver transplantation face rapid, universal reinfection of the graft. Therefore, antiviral strategies targeting the early stages of infection are urgently needed for the prevention of HCV infection. In this study, we identified the polyphenol, epigallocatechin-3-gallate (EGCG), as an inhibitor of HCV entry. Green tea catechins, such as EGCG and its derivatives, epigallocatechin (EGC), epicatechin gallate (ECG), and epicatechin (EC), have been previously found to exert antiviral and antioncogenic proper- ties. EGCG had no effect on HCV RNA replication, assembly, or release of progeny viri- ons. However, it potently inhibited Cell-culture–derived HCV (HCVcc) entry into hepatoma cell lines as well as primary human hepatocytes. The effect was independent of the HCV genotype, and both infection of cells by extracellular virions and cell-to-cell spread were blocked. Pretreatment of cells with EGCG before HCV inoculation did not reduce HCV infection, whereas the application of EGCG during inoculation strongly inhibited HCV infectivity. Moreover, treatment with EGCG directly during inoculation strongly inhibited HCV infectivity. -
Brief Clinical Evaluation of Six High-Throughput SARS-Cov-2 Igg Antibody T Assays Niko Kohmera, Sandra Westhausa, Cornelia Rühla, Sandra Cieseka,B,C, Holger F
Journal of Clinical Virology 129 (2020) 104480 Contents lists available at ScienceDirect Journal of Clinical Virology journal homepage: www.elsevier.com/locate/jcv Brief clinical evaluation of six high-throughput SARS-CoV-2 IgG antibody T assays Niko Kohmera, Sandra Westhausa, Cornelia Rühla, Sandra Cieseka,b,c, Holger F. Rabenaua,* a Institute for Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany b German Centre for Infection Research, External Partner Site, Frankfurt, Germany c Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch Translational Medicine and Pharmacology, Frankfurt, Germany ARTICLE INFO ABSTRACT Keywords: Serological SARS-CoV-2 assays are urgently needed for diagnosis, contact tracing and for epidemiological stu- SARS-CoV-2 dies. So far, there is limited data on how recently commercially available, high-throughput immunoassays, using IgG different recombinant SARS-CoV-2 antigens, perform with clinical samples. Focusing on IgG and totalanti- Antibody bodies, we demonstrate the performance of four automated immunoassays (Abbott Architect™ i2000 (N protein- Assay based)), Roche cobas™ e 411 analyzer (N protein-based, not differentiating between IgA, IgM or IgG antibodies), Evaluation LIAISON®XL platform (S1 and S2 protein-based), VIRCLIA® automation system (S1 and N protein-based) in PRNT comparison to two ELISA assays (Euroimmun SARS-CoV-2 IgG (S1 protein-based) and Virotech SARS-CoV-2 IgG ELISA (N protein-based)) and an in-house developed plaque reduction neutralization test (PRNT). We tested follow up serum/plasma samples of individuals PCR-diagnosed with COVID-19. When calculating the overall sensitivity, in a time frame of 49 days after first PCR-positivity, the PRNT as gold standard, showed the highest sensitivity with 93.3% followed by the dual-target assay for the VIRCLIA® automation system with 89%. -
Intranasal Administration of a Monoclonal Neutralizing Antibody Protects Mice Against SARS-Cov-2 Infection
viruses Article Intranasal Administration of a Monoclonal Neutralizing Antibody Protects Mice against SARS-CoV-2 Infection Sandro Halwe 1,2 , Alexandra Kupke 1,2 , Kanika Vanshylla 3 , Falk Liberta 4 , Henning Gruell 3 , Matthias Zehner 3, Cornelius Rohde 1,2 , Verena Krähling 1,2, Michelle Gellhorn Serra 1,2 , Christoph Kreer 3 , Michael Klüver 1,2 , Lucie Sauerhering 1,2, Jörg Schmidt 1,2 , Zheng Cai 5, Fei Han 5, David Young 5, Guangwei Yang 5, Marek Widera 6 , Manuel Koch 7,8 , Anke Werner 1 , Lennart Kämper 1, Nico Becker 1, Michael S. Marlow 5, Markus Eickmann 1, Sandra Ciesek 6,9,10 , Felix Schiele 4, Florian Klein 3,7,11 and Stephan Becker 1,2,* 1 Institute of Virology, Philipps University Marburg, Hans-Meerwein-Straße 2, 35043 Marburg, Germany; [email protected] (S.H.); [email protected] (A.K.); [email protected] (C.R.); [email protected] (V.K.); [email protected] (M.G.S.); [email protected] (M.K.); [email protected] (L.S.); [email protected] (J.S.); [email protected] (A.W.); [email protected] (L.K.); [email protected] (N.B.); [email protected] (M.E.) 2 German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany 3 Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany; [email protected] (K.V.); [email protected] (H.G.); [email protected] (M.Z.); [email protected] (C.K.); fl[email protected] (F.K.) Citation: Halwe, S.; Kupke, A.; 4 Biotherapeutics Discovery, Boehringer Ingelheim Pharma GmbH & Co. -
Analysis of Humoral Immune Responses in Patients with Severe
applyparastyle “fig//caption/p[1]” parastyle “FigCapt” The Journal of Infectious Diseases MAJOR ARTICLE Downloaded from https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiaa680/5944723 by Instituut Voor Tropische Geneeskunde, Bibliotheek user on 29 November 2020 Analysis of Humoral Immune Responses in Patients With Severe Acute Respiratory Syndrome Coronavirus 2 Infection Lisa Henss,1 Tatjana Scholz,1 Christine von Rhein,1 Imke Wieters,3 Frauke Borgans,3 Fabian J. Eberhardt,3 Kai Zacharowski,4 Sandra Ciesek,5 Gernot Rohde,6 Maria Vehreschild,3 Christoph Stephan,3 Timo Wolf,3, Heike Hofmann-Winkler,7 Heinrich Scheiblauer,2 and Barbara S. Schnierle1 1Paul-Ehrlich-Institut, Department of Virology, Langen, Germany, 2Paul-Ehrlich-Institut, IVD, Langen, Germany, 3Zentrum für Innere Medizin, Infektiologie, Universitätsklinikum Frankfurt, Goethe Universität Frankfurt, Frankfurt am Main, Germany, 4Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie, Universitätsklinikum Frankfurt, Frankfurt am Main, Germany, 5Institut für Medizinische Virologie, Universitätsklinikum Frankfurt am Main, Goethe Universität Frankfurt, Frankfurt am Main, Germany, 6Medizinische Klinik 1, Pneumologie/Allergologie, Universitätsklinikum Frankfurt–Goethe-Universität, Frankfurt am Main, Germany, and 7Deutsches Primatenzentrum GmbH, Leibniz-Institut für Primatenforschung, Göttingen, Germany Background. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has caused a pandemic with tens of millions of cases and hundreds of thousands of deaths. The infection causes coronavirus disease 2019 (COVID-19), a disease of the respiratory system of divergent severity. In the current study, humoral immune responses were characterized in a cohort of 143 patients with COVID-19 from the University Hospital Frankfurt am Main, Germany. Methods. SARS-CoV-2-specific–antibodies were detected by enzyme-linked immunosorbent assay (ELISA). -
Assessment of SARS-Cov-2 Transmission on an International Flight and Among a Tourist Group
Research Letter | Public Health Assessment of SARS-CoV-2 Transmission on an International Flight and Among a Tourist Group Sebastian Hoehl, MD; Onur Karaca; Niko Kohmer, MD; Sandra Westhaus, PhD; Jürgen Graf, MD; Udo Goetsch, MD; Sandra Ciesek, MD Introduction This case series assessed a commercial airline flight from Tel Aviv, Israel, to Frankfurt, Germany, that Author affiliations and article information are occurred on March 9th, 2020. Among 102 passengers on a Boeing 737-900 aircraft were 24 listed at the end of this article. members of a tourist group. Starting 7 days earlier, the group had contact with a hotel manager who later received a diagnosis of coronavirus disease 2019 (COVID-19). No member of the group had received a diagnosis of COVID-19 before the flight, and no measures to prevent transmission (eg, wearing of masks) had been applied. The flight duration was 4 hours 40 minutes. Methods At the destination airport, we conducted a medical evaluation of the tourist group, including testing for severe acute respiratory syndrome coronavirus (SARS-CoV-2) in a throat swab specimen. In addition, we contacted all passengers 4 to 5 weeks later by phone and conducted structured interviews. Passengers were asked whether they had contact with a person with COVID-19. They were prompted to report symptoms and asked about previous testing for SARS-CoV-2. A semiquantitative SARS-CoV-2 IgG antibody test (EUROIMMUN) was offered to all passengers who had been seated within 2 rows of the index cases and to those who reported to have been symptomatic. Borderline and positive results in the IgG test were confirmed with a plaque reduction neutralization test (PRNT). -
Coronavirus Pandemic: Use the End of Year Holidays to Impose a Strict Lockdown
7th Ad-hoc Statement – 08 December 2020 Coronavirus pandemic: Use the end of year holidays to impose a strict lockdown Current developments with regard to the coronavirus pandemic are a cause of major concern. Despite the prospect of vaccinations commencing in the near future, from a scientific perspective it is essential to impose a strict lockdown in order to rapidly and significantly reduce the number of new infections, which is still far too high. The lockdown should be implemented in two steps and apply to all of Germany. From 14 December 2020 onwards, pupils should not be obliged to attend school in person, and everyone who can work from home should be urged to do so. In addition, from 24 December 2020, all shops which do not supply food, medication, or other essential items should be closed. Social contacts should be reduced to a very small circle. The current situation is highly precarious and threatens to deteriorate further. Despite the partial lockdown which was introduced at the start of November, infection numbers are still far too high. An increasing number of elderly people are becoming infected. In the last seven days, more people have died with the coronavirus than died in traffic accidents throughout 2019. Hospitals, and in particular medical staff, are already at the limit of their capacities. The health system and major hospitals are not designed to cope with such high and prolonged demand. The health authorities, which play a key role in controlling the pandemic on a local level, are overburdened. In many cases, effective contact tracing is no longer possible. -
Frankfurt Researchers Discover Potential Targets for COVID-19 Therapy Jennifer Hohensteiner Public Relations Und Kommunikation Goethe-Universität Frankfurt Am Main
Frankfurt researchers discover potential targets for COVID-19 th... https://idw-online.de/de/news?print=1&id=747356 idw - Informationsdienst Wissenschaft 14.05.2020 11:00 Frankfurt researchers discover potential targets for COVID-19 therapy Jennifer Hohensteiner Public Relations und Kommunikation Goethe-Universität Frankfurt am Main Cell culture model: several compounds stop SARS-CoV-2 virus A team of biochemists and virologists at Goethe University and the Frankfurt University Hospital were able to observe how human cells change upon infection with SARS-CoV-2, the virus causing COVID-19 in people. The scientists tested a series of compounds in laboratory models and found some which slowed down or stopped virus reproduction. These results now enable the search for an active substance to be narrowed down to a small number of already approved drugs. (Nature DOI: 10.1038/s41586-020-2332-7). Based on these findings, a US company reports that it is preparing clinical trials. A Canadian company is also starting a clinical study with a different substance. Since the start of February, the Medical Virology of the Frankfurt University Hospital has been in possession of a SARS-CoV-2 infection cell culture system. The Frankfurt scientists in Professor Sandra Ciesek’s team succeeded in cultivating the virus in colon cells from swabs taken from two infected individuals returning from Wuhan (Hoehl et al. NEJM 2020). Using a technique developed at the Institute for Biochemistry II at Goethe University Frankfurt, researchers from both institutions were together able to show how a SARS-CoV-2 infection changes the human host cells. -
FACT- Frankfurt Adjusted COVID-19 Testing- a Novel Method Enables High-Throughput SARS-Cov-2 Screening Without Loss of Sensitivity
medRxiv preprint doi: https://doi.org/10.1101/2020.04.28.20074187; this version posted May 1, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . FACT- Frankfurt adjusted COVID-19 testing- a novel method enables high-throughput SARS-CoV-2 screening without loss of sensitivity Michael Schmidt, MD1, Sebastian Hoehl, MD2, Annemarie Berger, PhD2, Heinz Zeichhardt, MD3, Kai Hourfar, MD1, Sandra Ciesek, MD2,4*, Erhard Seifried, MD1* *These authors contributed equally 1German Red Cross Blood Transfusion Service, Frankfurt, Germany. 2 Institute for Medical Virology, University Hospital, Goethe-University, Frankfurt, Germany 3 Institut fuer Qualitaetssicherung in der Virusdiagnostik - IQVD der GBD mbH, Berlin, Germany; INSTAND Gesellschaft zur Foerderung der Qualitaetssicherung in medizinischen Laboratorien e.V., Duesseldorf, Germany; Charité-Universitaetsmedizin, Institut fuer Virologie, Berlin, Germany. 4 Germany Centre for Infection Research (DZIF), external partner site Frankfurt, Germany Address for Correspondence: Prof. Dr. Erhard Seifried German Red Cross Blood Transfusion Service Sandhofstraße 1 60528 Frankfurt am Main Germany [email protected] Prof Dr. Sandra Ciesek Institute for Medical Virology University Hospital Paul-Ehrlich-Str. 40 60596 Frankfurt Germany [email protected] Word count (manuscript): 1463 NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice. medRxiv preprint doi: https://doi.org/10.1101/2020.04.28.20074187; this version posted May 1, 2020.