DOCSLIB.ORG

Bacterial Chromosome Organisation and Transcription

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Bacterial Chromosome Organisation and Transcription Bacterial Chromosome Organisation and Transcription by Laura Sellars A thesis submitted to The University of Birmingham for the degree of DOCTOR OF PHILOSOPHY School of Biosciences The University of Birmingham April 2014 University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. Abstract The bacterial chromosome has to be condensed to fit inside the cell, forming a compact structure called the nucleoid, which is confined to a particular region of the cell without constriction by a membrane. Originally, the nucleoid was thought to be packed into the cell in a disordered way, unlike the highly organised chromatin of eukaryotic cells. More recently, the bacterial nucleoid has been shown to be far more structured than previously thought, with DNA present in topologically distinct loops, which are then arranged into macrodomains. Some of the proteins involved in structuring the E. coli chromosome are also known to have important roles in regulating transcription, and at least one transcription factor is known to cause distant DNA sites to cluster upon binding. These factors lead to the idea that chromosome structure could be affected by local gene expression. To investigate the possible link between chromosome structure and gene expression, Fluorescent Reporter/Operator Systems (FROS) were used to study the positions of different inducible promoters, with and without induction. The FROS method was adapted to use a smaller insert, therefore causing less disruption to the chromosome structure. The transcription factor MalI was also developed as a novel FROS reporter. Of the five promoters studied, only araFGH showed any movement upon induction, moving away from the cell pole. In cells at the point of division, induction of the araFGH promoter caused segregation of sister chromatids adjacent to araFGH. These results suggest that induction of promoters can cause a change in local chromosome structure, although this is not seen at all promoters. The diffusion of fluorescently tagged transcription factors, used as reporters in FROS, can also be studied using super resolution microscopy methods. Acknowledgements Over the 4 years it has taken to complete this thesis I have had help and advice from many people for which I am very grateful. Firstly I would like to thank my supervisor, Prof Steve Busby for giving me this opportunity and for his support and guidance over the past few years. I would also like to thank Dr Dave Lee, Rita Godfrey and Dr Doug Browning for sharing their experience and wisdom with me and helping me with all my practical problems. Thank you to Dr Jack Bryant for introducing me to the lab and spending a lot of his time helping me and discussing our projects. Thanks also to Dr Maritoñi Sánchez Romero for her patience and assistance in training me on the microscope, without which this project would never have got started. A massive thank you to Dr Eugenio Sanchez-Moran and his lab for allowing me to use their microscope and helping me with my problems, as well as making me feel very welcome. For the PALM experiments in Chapter 5, I thank Dr Achillefs Kapanidis and Federico Garza de Leon for carrying out the experiments. I look forward to continuing this collaboration. I would like to thank Stephen Bevan for constructing the strains for studying MntR controlled promoters used in Chapter 4, and also for being an excellent student and causing me very little trouble. I thank all members of the Busby and Cole labs, past and present, for their support and friendship. It has made the past 4 years a lot more pleasant than it otherwise would have been. I would like to thank my family, especially my parents and Lindsey for all of their encouragement through the whole of my life. I hope I’ve made you proud. Finally, I thank Andy, for getting me through this especially stressful year. Your turn next, then we will have a rest! Table of Contents 1. Introduction .......................................................................................................................... 1 1.1 Escherichia coli ................................................................................................................. 2 1.2 Chromosome Structure in Escherichia coli ...................................................................... 4 1.2.1 The Nucleoid .............................................................................................................. 5 1.2.2 Macrodomains ............................................................................................................ 8 1.2.3 Compaction of DNA ................................................................................................ 11 1.3 DNA replication and chromosome segregation in Escherichia coli ............................... 14 1.3.1 DNA replication ....................................................................................................... 14 1.3.2 Chromosome Segregation ........................................................................................ 18 1.4 Regulation of transcription in Escherichia coli .............................................................. 25 1.4.1 Bacterial RNA polymerase....................................................................................... 25 1.4.2 Promoter recognition ................................................................................................ 27 1.4.3 Transcription factors ................................................................................................ 30 1.4.4 Link between nucleoid structure and transcription regulation ................................. 34 1.5 Aims of the project ......................................................................................................... 35 2. Materials and Methods ...................................................................................................... 38 2.1 Buffers, Solutions and Reagents ..................................................................................... 39 2.2 Growth Media ................................................................................................................. 39 2.2.1 Solid Media .............................................................................................................. 39 2.2.2 Liquid Media ............................................................................................................ 39 2.2.3 Antibiotics and other supplements ........................................................................... 40 2.3 Bacterial Strains .............................................................................................................. 41 2.4 Plasmids .......................................................................................................................... 41 2.5 Gel electrophoresis of DNA ........................................................................................... 63 2.5.1 Agarose gel electrophoresis ..................................................................................... 63 2.5.2 Polyacrylamide gel electrophoresis .......................................................................... 64 2.6 Extraction and purification of nucleic acids ................................................................... 64 2.6.1 Purification of DNA using QIAquick PCR purification kit ..................................... 64 2.6.2 Extraction of DNA from agarose gels ...................................................................... 65 2.6.3 Preparation of plasmid DNA .................................................................................... 65 2.7 Transformations .............................................................................................................. 65 2.7.1 Preparation of competent cells using the calcium chloride method ......................... 65 2.7.2 Transformation ......................................................................................................... 66 2.8 DNA Manipulations ........................................................................................................ 66 2.8.1 Standard PCR ........................................................................................................... 66 2.8.2 Colony PCR ............................................................................................................. 73 2.8.3 Restriction Digestion of DNA .................................................................................. 73 2.8.4 DNA Ligation ........................................................................................................... 75 2.8.5 DNA Sequencing...................................................................................................... 75 2.9 Gene Doctoring ............................................................................................................... 75 2.9.1 Using homologous recombination to make chromosomal modifications ................ 75 2.9.2 Gene doctoring protocol ..........................................................................................
Load more

Recommended publications
  • Identification and Characterisation of Cell Division Proteins in Staphylococcus Aureus
    Identification and Characterisation of Cell Division Proteins in Staphylococcus aureus By Azhar F. Kabli MSc (University of Sheffield) A thesis submitted for the degree of Doctor of Philosophy December 2013 Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN i Summary Cell division is a vital process that is required for bacterial proliferation and is thus an important target for the development of new antimicrobial agents. Bacterial cell division has mainly been studied in rod-shaped microorganisms, where a complex macromolecular machine, termed the divisome, mediates the division process. Cell division requires the coordination of components from the cytoplasm, through the membrane, to the cell wall where synthesis of new peptidoglycan takes place. Escherichia coli and Bacillus subtilis divisomes involve multiple essential components, mostly of unknown function. Staphylococcus aureus is a coccus that divides by binary fission in three orthogonal planes. The cell division machinery of S. aureus has been initially mapped as it is a clinically significant pathogen that poses a serious threat to public health due to resistance to current antibiotics. Indeed, the search for new drug targets against S. aureus is crucial. In this study, S. aureus cell division components DivIC and FtsL were identified as members of a novel class of cell wall-binding proteins, and their affinity for the cell wall was shown to be enhanced by the presence of wall teichoic acids. A GFP fusion analysis and immunolocalisation experiments demonstrated that DivIC and FtsL may transiently localise to the division site and their localisation patterns suggest that they may identify previous or potential planes of division by recognising specific forms of peptidoglycan architecture.
    [Show full text]
  • Tom 46 Zeszyt 1 Rok 2007
    RADA REDAKCYJNA JACEK BIELECKI (Uniwersytet Warszawski), MIECZYS£AW K. B£ASZCZYK (SGGW Warszawa), RYSZARD CHRÓST (Uniwersytet Warszawski), JERZY D£UGOÑSKI (Uniwersytet £ódzki), DANUTA DZIER¯ANOWSKA (Centrum Zdrowia Dziecka), EUGENIA GOSPODAREK (Collegium Medicum UMK w Bydgoszczy), JERZY HREBENDA (Uniwersytet Warszawski), WALERIA HRYNIEWICZ (Narodowy Instytut Leków), MAREK JAKÓBISIAK (Akademia Medyczna w Warszawie), MIROS£AW KAÑTOCH (Pañstwowy Zak³ad Higieny), ANDRZEJ PASZEWSKI (Instytut Biochemii i Biofizyki PAN), ANDRZEJ PIEKAROWICZ (Uniwersytet Warszawski), ANTONI RÓ¯ALSKI (Uniwersytet £ódzki), BOHDAN STAROCIAK (Akademia Medyczna w Warszawie), BOGUS£AW SZEWCZYK (Uniwersytet Gdañski), EL¯BIETA TRAFNY (Wojskowy Instytut Higieny i Epidemiologii), STANIS£AWA TYLEWSKA-WIERZBANOWSKA (Pañstwowy Zak³ad Higieny), GRZEGORZ WÊGRZYN (Uniwersytet Gdañski), PIOTR ZIELENKIEWICZ (Uniwersytet Warszawski) REDAKCJA JERZY HREBENDA (redaktor naczelny), JACEK BIELECKI (zastêpca), BOHDAN STAROCIAK (sekretarz), MARTA BRZÓSTKOWSKA (korekta tekstów angielskich) Adresy redakcji Redaktorzy: Instytut Mikrobiologii, Wydzia³ Biologii, Uniwersytet Warszawski ul. Miecznikowa 1, 02-096 Warszawa, tel. (0 22) 554 13 05/304, fax (0 22) 554 14 04 e-mail: [email protected]; [email protected] Sekretarz Zak³ad Mikrobiologii Farmaceutycznej, Akademia Medyczna ul. Oczki 3 (parter), 02-007 Warszawa, tel. (0 22) 628 08 22, (0 22) 621 13 51 e-mail: [email protected] PUBLIKACJE METODYCZNE I STANDARDY Redaktor odpowiedzialny: STEFANIA GIEDRYS-KALEMBA (Pomorska
    [Show full text]
  • Structure and Function of the Bacterial Genome
    k Structure and Function of the Bacterial Genome k k k k Structure and Function of the Bacterial Genome Charles J. Dorman Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland k k k k This edition first published 2020 © 2020 John Wiley & Sons, Inc. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by law. Advice on how to obtain permission to reuse material from this title is available at http://www.wiley.com/go/permissions. The right of Charles J. Dorman to be identified as the author of this work has been asserted in accordance with law. Registered Office John Wiley & Sons, Inc., 111 River Street, Hoboken, NJ 07030, USA Editorial Office Boschstr. 12, 69469 Weinheim, Germany For details of our global editorial offices, customer services, and more information about Wiley products visit us at www.wiley.com. Wiley also publishes its books in a variety of electronic formats and by print-on-demand. Some content that appears in standard print versions of this book may not be available in other formats. Limit of Liability/Disclaimer of Warranty While the publisher and authors have used their best efforts in preparing this work, they make no representations or warranties with respect to the accuracy or completeness of the contents of this work and specifically disclaim all warranties, including without limitation any implied warranties of merchantability or fitness for a particular purpose.
    [Show full text]
  • UCLA Electronic Theses and Dissertations
    UCLA UCLA Electronic Theses and Dissertations Title Regulation of Cytokinetic Protein FtsZ in Caulobacter crescentus by Elements of the Chromosome Partitioning System Permalink https://escholarship.org/uc/item/9r60p8rn Author Graf, Matthew Allan Gaffney Publication Date 2014 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA Los Angeles Regulation of Cytokinetic Protein FtsZ in Caulobacter crescentus by Elements of the Chromosome Partitioning System A dissertation submitted in partial satisfaction of the requirements of the degree Doctor of Philosophy in Biochemistry and Molecular Biology by Matthew Allan Gaffney Graf 2014 © Copyright by Matthew Allan Gaffney Graf 2014 ABSTRACT OF THE DISSERTATION Regulation of cytokinetic protein FtsZ in Caulobacter crescentus by elements of the chromosome partitioning system by Matthew Allan Gaffney Graf Doctor of Philosophy in Biochemistry and Molecular Biology University of California, Los Angeles, 2014 Professor James Gober, Chair The coordination of essential cell cycle events is necessary to maintain the viability of a species over many generations; two core processes to any replicative cell is the duplication of chromosomal DNA and the division of a growing cell into two functional daughters. The crescent-shaped α-proteobacteria, Caulobacter crescentus, has a distinct biphasic life cycle making it ideal to study such cell cycle linked events. The core protein of cell division in C. crescentus is the essential, and highly conserved tubulin-homologue, FtsZ. Prior to FtsZ directed cell division, duplicating chromosomes are quickly sequestered to opposing regions of the replicative cell such that each daughter will contain a complete chromosome after division.
    [Show full text]
  • Biogenesis of Shigella Flexneri Icsa Protein
    marcin grabowicz Biogenesis of Shigella flexneri IcsA Protein Biogenesis of Shigella flexneri IcsA Protein marcin grabowicz, b.sc.(hons.) Submitted for the degree of Doctor of Philosophy Discipline of Microbiology and Immunology School of Molecular and Biomedical Science The University of Adelaide March, 2010 Declaration This work contains no material which has been accepted for the award of any other degree or diploma in any university or other tertiary institution to Marcin Grabowicz and, to the best of my knowledge and belief, contains no material previously published or written by another person, except where due reference has been made in the text. I give consent to this copy of my thesis, when deposited in the University Library, being made available for loan and photocopying, subject to the provisions of the Copyright Act 1968. I also give permission for the digital version of my thesis to be made available on the web, via the University’s digital research repository, the Library catalogue, the Australasian Digital Theses Program (ADTP) and also through web search engines, unless permission has been granted by the University to restrict access for a period of time. Adelaide, Australia, March, 2010 Marcin Grabowicz Abstract The IcsA autotransporter is a vital virulence factor for Shigella flexneri, a human- specific causative agent of bacillary dysentery that accounts for over a million global deaths annually. Ingested Shigellae invade and spread throughout the colonic epithelium. IcsA confers motility to intracellular bacteria by engaging host actin regulatory proteins to polymerise filaments of actin in a processes termed actin-based motility. This IcsA-dependent motility potentiates the intercellular spreading.
    [Show full text]
  • Transient Non-Specific DNA Binding Dominates the Target Search of Bacterial DNA-Binding 2 Proteins
    bioRxiv preprint doi: https://doi.org/10.1101/2020.08.13.249771; this version posted August 13, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Transient non-specific DNA binding dominates the target search of bacterial DNA-binding 2 proteins 3 4 Mathew Stracy*a, Jakob Schweizerb, David J Sherratta, Achillefs N Kapanidisc, Stephan Uphoff*a, 5 Christian Lesterlin*d 6 aDepartment of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom 7 bMax Planck Institute for Dynamics of Complex Technical Systems, Magdeburg 8 cBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of 9 Oxford, Oxford OX1 3PU, United Kingdom 10 dMolecular Microbiology and Structural Biochemistry (MMSB), Université Lyon 1, CNRS, Inserm, 11 UMR5086, 69007, Lyon, France 12 13 *Correspondence to: [email protected]; [email protected] 14 [email protected] bioRxiv preprint doi: https://doi.org/10.1101/2020.08.13.249771; this version posted August 13, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 15 ABSTRACT 16 Despite their diverse biochemical characteristics and functions, all DNA-binding proteins share 17 the ability to accurately locate their target sites among the vast excess of non-target DNA.
    [Show full text]
  • WO 2018/201161 Al 01 November 2018 (01.11.2018) W !P O PCT
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/201161 Al 01 November 2018 (01.11.2018) W !P O PCT (51) International Patent Classification: Zachery, George; 3 125 District Ave., #324, Char C12N 1/20 (2006.01) C12N 15/79 (2006.01) lottesville, VA 22901 (US). POURTAHERI, Payam; 4000 CI2N 1/21 (2006.01) City Walk Way, #213, Charlottesville, VA 22902 (US). (21) International Application Number: (74) Agent: HOLLY, David, Christopher et al; 1299 Penn syl PCT/US20 18/030329 vania Avenue NW, Suite 700, Washington, DC 20004-2400 (US). (22) International Filing Date: 30 April 2018 (30.04.2018) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (25) Filing Language: English AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (26) Publication Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (30) Priority Data: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 62/491,608 28 April 2017 (28.04.2017) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 62/570,368 10 October 2017 (10.10.2017) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (71) Applicant: AGROSPHERES, INC. [US/US]; 1180 Semi OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, nole Trail, Suite 100, Charlottesville, VA 22901 (US).
    [Show full text]
  • Identification of Escherichia Coli Genes Required For
    IDENTIFICATION OF ESCHERICHIA COLI GENES REQUIRED FOR BACTERIAL SURVIVAL AND MORPHOLOGICAL PLASTICITY IN URINARY TRACT INFECTIONS DANIEL GIUSEPPE MEDIATI A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy The ithree institute and School of Life Sciences, University of Technology Sydney November 2018 [blank page] Production Note: Signature removed prior to publication. iv TO MUM AND DAD, WHO INSTILLED IN ME THE GREAT VALUE OF EDUCATION. v CONTENTS IN BRIEF CHAPTER 1: INTRODUCTION CHAPTER 2: GENERAL MATERIALS AND METHODS CHAPTER 3: HIGH-THROUGHPUT SEQUENCING OF SORTED EXPRESSION LIBRARIES REVEALS INHIBITORS OF BACTERIAL CELL DIVISION CHAPTER 4: GENOME-WIDE COMPARISON OF E. COLI GENES REQUIRED FOR GROWTH IN COMPLEX AND MINIMAL MEDIA CHAPTER 5: THE ESSENTIAL GENE PROFILE SET OF UROPATHOGENIC E. COLI DURING BLADDER CELL INFECTION CHAPTER 6: DISCUSSION AND CONCLUSION REFERENCES vi TABLE OF CONTENTS Certificate of authorship and originality iii List of figures and supplementary data x List of tables and supplementary data xi Abbreviations xii Publications xiv Conference proceedings xv Acknowledgements xvi Preface xvii CHAPTER 1: INTRODUCTION 1 1.1 Urinary tract infections 3 1.1.1 Uropathogenic Escherichia coli 4 1.2 UPEC lifestyle from the intestine to the urinary tract 6 1.3 Infection cycle events in the bladder 8 1.3.1 Bacterial attachment and invasion of host cells 8 1.3.2 Intracellular bacterial communities 13 1.3.3 UPEC dispersal and filamentation 16 1.3.3.1 E. coli cell division and filamentation
    [Show full text]
  • PAULA MARIA MOREIRA MARTINS Julho
    UNIVERSIDADE ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO” unesp INSTITUTO DE BIOCIÊNCIAS – RIO CLARO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS (MICROBIOLOGIA APLICADA) PAPEL DA PROTEÍNA ZapA NA DIVISÃO CELULAR E PATOGENICIDADE DE Xanthomonas citri subsp. citri PAULA MARIA MOREIRA MARTINS Tese apresentada ao Instituto de Biociências do Câmpus de Rio Claro, Universidade Estadual Paulista, como parte dos requisitos para obtenção do título de Doutor em Ciências Biológicas (Microbiologia Aplicada). Julho - 2013 PAULA MARIA MOREIRA MARTINS PAPEL DA PROTEÍNA ZapA NA DIVISÃO CELULAR E PATOGENICIDADE DE Xanthomonas citri subsp. citri Tese apresentada ao Instituto de Biociências do Câmpus de Rio Claro, Universidade Estadual Paulista, como parte dos requisitos para obtenção do título de Doutor em Ciências Biológicas (Microbiologia Aplicada) Orientador: Prof. Dr. Henrique Ferreira Co-orientador: Dr. Alexandre Morais do Amaral Rio Claro 2013 576 Martins, Paula Maria Moreira M386p Papel da proteína ZapA na divisão celular e patogenicidade de Xanthomonas citri subsp. citri / Paula Maria Moreira Martins. - Rio Claro, 2013 87 f. : il., figs., tabs., quadros Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro Orientador: Henrique Ferreira Coorientador: Dr. Alexandre Morais do Amaral 1. Microorganismos. 2. Cancro cítrico. 3. Septo. 4. Bactéria. 5. Citricultura. 6. XAC3407. I. Título. Ficha Catalográfica elaborada pela STATI - Biblioteca da UNESP Campus de Rio Claro/SP Aos meus pais e noivo por todo o suporte que recebi ao longo destes anos DEDICO AGRADECIMENTOS Agradeço a todos os meus professores por me mostrarem o caminho, e a Deus pela força na difícil tarefa de seguir em frente. Aos meus pais pelo esforço, dedicação e carinho que sempre tiveram por mim e agradeço também ao meu noivo João, que sem dúvidas me tornou uma pessoa melhor.
    [Show full text]
  • Mind Proteolysis 010620
    bioRxiv preprint doi: https://doi.org/10.1101/2020.01.09.899195; this version posted January 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Disassembly and degradation of MinD oscillator complexes by Escherichia coli ClpXP Christopher J. LaBreck, Catherine E. Trebino, Colby N. Ferreira, Josiah J. Morrison, Eric C. DiBiasio, Joseph Conti, Jodi L. Camberg From the Department of Cell & Molecular Biology, The University of Rhode Island, Kingston, RI, 02881 Running title: Degradation of MinD in E. coli *To whom correspondence should be addressed: Jodi L. Camberg, 120 Flagg Road, Kingston, RI, 02881; Tel: (401)874-4961; Email: [email protected] Keywords: Cell division, Min system, MinD, ClpXP, AAA+ ATPase, Escherichia coli bioRxiv preprint doi: https://doi.org/10.1101/2020.01.09.899195; this version posted January 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract MinD is a cell division ATPase in Escherichia coli that oscillates from pole to pole and regulates the spatial position of the cell division machinery. Together with MinC and MinE, the Min system restricts assembly of the FtsZ-ring to midcell, oscillating between the opposite ends of the cell and preventing FtsZ-ring misassembly at the poles.
    [Show full text]
  • Molecular Genetics of Bacteria, 5Th Edition
    Molecular Genetics of Bacteria 5th edition Jeremy W. Dale and Simon F. Park University of Surrey, UK A John Wiley & Sons, Ltd., Publication Molecular Genetics of Bacteria 5th edition Molecular Genetics of Bacteria 5th edition Jeremy W. Dale and Simon F. Park University of Surrey, UK A John Wiley & Sons, Ltd., Publication This edition first published 2010, 2010 by John Wiley & Sons, Ltd Wiley-Blackwell is an imprint of John Wiley & Sons, formed by the merger of Wiley’s global Scientific, Technical and Medical business with Blackwell Publishing. Registered office: John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK Other Editorial Offices: 9600 Garsington Road, Oxford, OX4 2DQ, UK 111 River Street, Hoboken, NJ 07030-5774, USA For details of our global editorial offices, for customer services and for information about how to apply for permission to reuse the copyright material in this book please see our website at www.wiley.com/wiley-blackwell The right of the author to be identified as the author of this work has been asserted in accordance with the Copyright, Designs and Patents Act 1988. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by the UK Copyright, Designs and Patents Act 1988, without the prior permission of the publisher. Wiley also publishes its books in a variety of electronic formats. Some content that appears in print may not be available in electronic books.
    [Show full text]
  • Stability, Placement and Interactions of the Divisome Components in Nucleoid-Deprived Escherichia Coli Cells
    Universidad Autónoma de Madrid Facultad de Ciencias Departamento de Biología Molecular Stability, placement and interactions of the divisome components in nucleoid-deprived Escherichia coli cells TESIS DOCTORAL Memoria presentada para optar al grado de Doctor en Ciencias Manuel Pazos Don Pedro TUTOR ACADÉMICO: DIRECTORES: José Berenguer Carlos Miguel Vicente Muñoz Paolo Natale Consejo Superior de Investigaciones Científicas Centro Nacional de Biotecnología Madrid, 2013 Cover: Fluorescence microscopy image of CSR603 cells overproducing FtsZ*-VM Este trabajo se ha realizado en el Centro Nacional de Biotecnología (CNB-CSIC) bajo la dirección del Doctor Paolo Natale y del Profesor de Investigación Miguel Vicente Muñoz, y en la Universidad de Texas (Houston, EEUU) durante una estancia de 6 meses en el laboratorio del Profesor William Margolin. Ha sido financiado por el programa de Formación de Personal Investigador del Ministerio de Educación y Ciencia. Agradecimientos: - A Miguel, por darme la oportunidad y los medios para poder realizar esta tesis doctoral y haberme animado, ayudado y enseñado a ser mejor científico en estos años. - A Paolo, por todo el tiempo y el esfuerzo que ha dedicado a este proyecto ("trabajando codo con codo"), por tener siempre tiempo para explicarme y enseñarme algo nuevo cada día, por su incansable espíritu crítico, por sus consejos, y por guiarme y ayudarme a mejorar esta tesis. - To William Margolin for all the good advices and help since I was in his lab, and to all the people in his lab (Daniel, Heather, Jennifer, Jesús, Jenny, Kim and Tushar) for making me feel like at home and having a lot of fun there.
    [Show full text]