2009-2010 Catalogue Peptide
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Evaluation of in Silico Approach for Prediction of Presence of Opioid Peptides in Wheat
Evaluation of in silico approach for prediction of presence of opioid peptides in wheat This is the Accepted version of the following publication Garg, Swati, Apostolopoulos, Vasso, Nurgali, Kulmira and Mishra, Vijay Kumar (2018) Evaluation of in silico approach for prediction of presence of opioid peptides in wheat. Journal of Functional Foods, 41. 34 - 40. ISSN 1756-4646 The publisher’s official version can be found at https://www.sciencedirect.com/science/article/pii/S1756464617307454 Note that access to this version may require subscription. Downloaded from VU Research Repository https://vuir.vu.edu.au/36577/ 1 1 Evaluation of in silico approach for prediction of presence of opioid peptides in wheat 2 gluten 3 Abstract 4 Opioid like morphine and codeine are used for the management of pain, but are associated 5 with serious side-effects limiting their use. Wheat gluten proteins were assessed for the 6 presence of opioid peptides on the basis of tyrosine and proline within their sequence. Eleven 7 peptides were identified and occurrence of predicted sequences or their structural motifs were 8 analysed using BIOPEP database and ranked using PeptideRanker. Based on higher peptide 9 ranking, three sequences YPG, YYPG and YIPP were selected for determination of opioid 10 activity by cAMP assay against µ and κ opioid receptors. Three peptides inhibited the 11 production of cAMP to varied degree with EC50 values of YPG, YYPG and YIPP were 5.3 12 mM, 1.5 mM and 2.9 mM for µ-opioid receptor, and 1.9 mM, 1.2 mM and 3.2 mM for κ- 13 opioid receptor, respectively. -
2019-2020 Award Recipients
2019-2020 AWARD RECIPIENTS The Office of Undergraduate Research and Creative Activities is pleased to announce the MAYS and RPG recipients for the 2019-2020 academic year. Please join us in congratulating these students and their faculty mentors. Major Academic Year Support (MAYS) Student: Kelly Ackerly Mentor: Dr. Daniel Greenberg Major: Psychology Department: Psychology An Exploration of Maternal Factors Affecting Children’s Autobiographical Memory In day-to-day interactions, mothers and their young children discuss memories of events they have experienced. Research has demonstrated the relationship between these interactions and the development of children’s memories. It is through these interactions that children learn how to interpret personal experiences and develop the skill of talking about them with others in a coherent way. Additionally, studies have found that children are more likely to form false memories—that is, inaccurate “memories” of events that did not actually occur – because their memories are more easily manipulated. In this study, we will explore whether the way mothers talk to their children about ambiguous events affects the children’s interpretations and memories of the event. We will also attempt to determine if there is a relationship between mothers’ negativity during discussions and their child’s formation of false memories. To explore this idea, mothers and their children (aged 3 to 6 years) are given a handful of ambiguous situations to interpret separately. Children are given the opportunity to make slime with a research assistant while a second research assistant acts out ambiguous situations that could have a positive or negative interpretation. The children are evaluated on the way they interpret the situations and whether they form a negative false memory. -
Proquest Dissertations
Opioid peptide permeation across the blood- brain and blood-cerebrospinal fluid barriers Item Type text; Dissertation-Reproduction (electronic) Authors Abbruscato, Thomas John, 1970- Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 04/10/2021 05:24:05 Link to Item http://hdl.handle.net/10150/282429 INFORMATION TO USERS This manuscript has been reproduced from the microfihn master. UMI fihns the text direct^ from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter &ce, while others may be from any type of computer printer. The quality of this reproductioii is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, b^inning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. -
Review Article NATRIURETIC PEPTIDES in CARDIOVASCULAR
CELLULAR & MOLECULAR BIOLOGY LETTERS http://www.cmbl.org.pl Received: 18 January 2007 Volume 13 (2008) pp 155-181 Revised form accepted: 08 May 2007 DOI: 10.2478/s11658-007-0046-6 Published online: 29 October 2007 © 2007 by the University of Wrocław, Poland Review article NATRIURETIC PEPTIDES IN CARDIOVASCULAR DISEASES MARIUSZ PIECHOTA1, MACIEJ BANACH2*, ANNA JACOŃ3 and JACEK RYSZ4 1Department of Anaesthesiology and Intensive Care Unit, Boleslaw Szarecki University Hospital No. 5 in Łódź, Medical University in Łódź, Poland, 2Department Cardiology, 1st Chair of Cardiology and Cardiac Surgery, University Hospital No. 3 in Łódź, Medical University in Łódź, Poland, 3Department of Health Protection Policy, Medical University of Łódź, Poland 42nd Department of Family Medicine, University Hospital No. 2 in Łódź, Medical University in Łódź, Poland Abstract: The natriuretic peptide family comprises atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), C-type natriuretic peptide (CNP), dendroaspis natriuretic peptide (DNP), and urodilatin. The activities of natriuretic peptides and endothelins are strictly associated with each other. ANP and BNP inhibit endothelin-1 (ET-1) production. ET-1 stimulates natriuretic peptide synthesis. All natriuretic peptides are synthesized from polypeptide precursors. Changes in natriuretic peptides and endothelin release were observed in many cardiovascular diseases: e.g. chronic heart failure, left ventricular dysfunction and coronary artery disease. Key words: Atrial natriuretic peptide, Brain natriuretic -
Pharmaceutical Nasal Compositions and Methods for Peptid Treatment
(19) TZZ ¥Z_T (11) EP 2 283 850 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: A61K 31/485 (2006.01) A61K 9/107 (2006.01) 25.04.2018 Bulletin 2018/17 A61K 9/08 (2006.01) A61K 38/02 (2006.01) A61K 38/25 (2006.01) A61K 31/12 (2006.01) (2006.01) (2006.01) (21) Application number: 10186116.9 A61K 31/365 A61K 9/00 A61K 47/32 (2006.01) A61K 47/22 (2006.01) A61K 47/06 (2006.01) A61K 47/08 (2006.01) (22) Date of filing: 07.03.2005 A61K 47/10 (2017.01) A61K 47/18 (2017.01) A61K 47/44 (2017.01) (54) Pharmaceutical nasal compositions and methods for peptid treatment Pharmazeutische nasale Zubereitungen und Peptidbehandlungen Compositions pharmaceutiques nasales et traitements aux peptides (84) Designated Contracting States: • Reppucci, Carl AT BE BG CH CY CZ DE DK EE ES FI FR GB GR North Andover, MA 01845 (US) HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR (74) Representative: Chajmowicz, Marion et al (30) Priority: 05.03.2004 US 895465 Becker & Associés 25, rue Louis Le Grand (43) Date of publication of application: 75002 Paris (FR) 16.02.2011 Bulletin 2011/07 (56) References cited: (62) Document number(s) of the earlier application(s) in WO-A-03/000158 accordance with Art. 76 EPC: 05724894.0 / 1 773 369 Remarks: Thefile contains technical information submitted after (73) Proprietor: CPEX Pharmaceuticals, Inc. the application was filed and not included in this Exeter, NH 03833 (US) specification (72) Inventors: • Gyurik, Robert Exeter, NH 03833 (US) Note: Within nine months of the publication of the mention of the grant of the European patent in the European Patent Bulletin, any person may give notice to the European Patent Office of opposition to that patent, in accordance with the Implementing Regulations. -
Opioid Imaging
529 NEUROIMAGING CLINICS OF NORTH AMERICA Neuroimag Clin N Am 16 (2006) 529–552 Opioid Imaging Alexander Hammers, PhDa,b,c,*, Anne Lingford-Hughes, PhDd,e - Derivation, release, peptide action, Changes in receptor availability in pain and metabolism and discomfort: between-group - Receptors and ligands comparisons Receptors Direct intrasubject comparisons of periods Species differences with pain and pain-free states Regional and layer-specific subtype - Opioid imaging in epilepsy distributions Focal epilepsies Ligands Idiopathic generalized epilepsy - Positron emission tomography imaging Summary of opioid receptors - Opioid imaging in other specialties Introduction PET imaging Movement disorders Quantification of images Dementia Available ligands and their quantification Cardiology - Opioid receptor imaging in healthy - Opioid imaging in psychiatry: addiction volunteers - Summary - Opioid imaging in pain-related studies - Acknowledgments - References Opioids derive their name from the Greek o´pioy agonists provided evidence for the existence of mul- for poppy sap. Various preparations of the opium tiple receptors [4]. In the early 1980s, there was ev- poppy Papaver somniferum have been used for pain idence for the existence of at least three types of relief for centuries. Structure and stereochemistry opiate receptors: m, k, and d [5,6]. A fourth ‘‘orphan’’ are essential for the analgesic actions of morphine receptor (ORL1 or NOP1) displays a high degree and other opiates, leading to the hypothesis of the of structural homology with conventional opioid existence of specific receptors. Receptors were iden- receptors and was identified through homology tified simultaneously by three laboratories in 1973 with the d receptor [7], but the endogenous ligand, [1–3]. The different pharmacologic activity of orphanin FQ/nociceptin, does not interact directly Dr. -
Opioid Peptides 49 Ryszard Przewlocki
Opioid Peptides 49 Ryszard Przewlocki Abbreviations ACTH Adrenocorticotropic hormone CCK Cholecystokinin CPA Conditioned place aversion CPP Conditioned place preference CRE cAMP response element CREB cAMP response element binding CRF Corticotrophin-releasing factor CSF Cerebrospinal fluid CTAP D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (m-opioid receptor antagonist) DA Dopamine DOP d-opioid peptide EOPs Endogenous opioid peptides ERK Extracellular signal-regulated kinase FSH Follicle-stimulating hormone GnRH Gonadotrophin-releasing hormone HPA axis Hypothalamo-pituitary-adrenal axis KO Knockout KOP k-opioid peptide LH Luteinizing hormone MAPK Mitogen-activated protein kinase MOP m-opioid peptide NOP Nociceptin opioid peptide NTS Nucleus tractus solitarii PAG Periaqueductal gray R. Przewlocki Department of Molecular Neuropharmacology, Institute of Pharmacology, PAS, Krakow, Poland Department of Neurobiology and Neuropsychology, Jagiellonian University, Krakow, Poland e-mail: [email protected] D.W. Pfaff (ed.), Neuroscience in the 21st Century, 1525 DOI 10.1007/978-1-4614-1997-6_54, # Springer Science+Business Media, LLC 2013 1526 R. Przewlocki PDYN Prodynorphin PENK Proenkephalin PNOC Pronociceptin POMC Proopiomelanocortin PTSD Posttraumatic stress disorder PVN Paraventricular nucleus SIA Stress-induced analgesia VTA Ventral tegmental area Brief History of Opioid Peptides and Their Receptors Man has used opium extract from poppy seeds for centuries for both pain relief and recreation. At the beginning of the nineteenth century, Serturmer first isolated the active ingredient of opium and named it morphine after Morpheus, the Greek god of dreams. Fifty years later, morphine was introduced for the treatment of postoper- ative and chronic pain. Like opium, however, morphine was found to be an addictive drug. -
The Role of Protein Convertases in Bigdynorphin and Dynorphin a Metabolic Pathway
Université de Montréal The Role of Protein Convertases in Bigdynorphin and Dynorphin A Metabolic Pathway par ALBERTO RUIZ ORDUNA Département de biomédecine vétérinaire Faculté de médecine vétérinaire Mémoire présenté à la Faculté de médecine vétérinaire en vue de l’obtention du grade de maître ès sciences (M.Sc.) en sciences vétérinaires option pharmacologie Décembre, 2015 © Alberto Ruiz Orduna, 2015 Résumé Les dynorphines sont des neuropeptides importants avec un rôle central dans la nociception et l’atténuation de la douleur. De nombreux mécanismes régulent les concentrations de dynorphine endogènes, y compris la protéolyse. Les Proprotéines convertases (PC) sont largement exprimées dans le système nerveux central et clivent spécifiquement le C-terminale de couple acides aminés basiques, ou un résidu basique unique. Le contrôle protéolytique des concentrations endogènes de Big Dynorphine (BDyn) et dynorphine A (Dyn A) a un effet important sur la perception de la douleur et le rôle de PC reste à être déterminée. L'objectif de cette étude était de décrypter le rôle de PC1 et PC2 dans le contrôle protéolytique de BDyn et Dyn A avec l'aide de fractions cellulaires de la moelle épinière de type sauvage (WT), PC1 -/+ et PC2 -/+ de souris et par la spectrométrie de masse. Nos résultats démontrent clairement que PC1 et PC2 sont impliquées dans la protéolyse de BDyn et Dyn A avec un rôle plus significatif pour PC1. Le traitement en C-terminal de BDyn génère des fragments peptidiques spécifiques incluant dynorphine 1-19, dynorphine 1-13, dynorphine 1-11 et dynorphine 1-7 et Dyn A génère les fragments dynorphine 1-13, dynorphine 1-11 et dynorphine 1-7. -
The Expression of Bradykinin and Its Receptors in Spinal Cord Ischemia
Life Sciences 218 (2019) 340–345 Contents lists available at ScienceDirect Life Sciences journal homepage: www.elsevier.com/locate/lifescie The expression of bradykinin and its receptors in spinal cord ischemia- T reperfusion injury rat model ⁎ Zheng Ma, Quan Dong, Boqiang Lyu, Jubo Wang, Yu Quan, Shouping Gong Department of Neurosurgery, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710014, PRChina ARTICLE INFO ABSTRACT Keywords: Objective: To investigate the expression and time-dependent manner of bradykinin (BK) as well as its receptors Spinal cord ischemia-reperfusion injury (Bradykinin receptors 1 and 2, B1R and B2R) in spinal cord ischemia-reperfusion injury (SCII) in rat model. Bradykinin Methods: Sprague-Dawley (SD) rats were subjected to 1 h of infra-renal abdominal aorta occlusion and re- Bradykinin receptors perfused for 3 h to 5 d to induce SCII. The concentration of BK in serum was detected by enzyme linked im- Kallikrein–kinin system munosorbent assay (ELISA). In situ expression of BK receptors was evaluated by immunochemistry and their mRNA level was evaluated by Real time quantitative-PCR (RTq-PCR). Results: The concentration of BK in serum was increased right after following SCII. Both of the BK receptors were detected and up-regulated in 24 h and 48 h after injury. The levels of B1R and B2R mRNA were up-regulated after SCII, and the B1R mRNA dropped to basal level after 6 h, but B2R mRNA dropped to lower level right after injury, peaked at 3 h, then remained a lower level from 6 h till 5 day. -
Five Decades of Research on Opioid Peptides: Current Knowledge and Unanswered Questions
Molecular Pharmacology Fast Forward. Published on June 2, 2020 as DOI: 10.1124/mol.120.119388 This article has not been copyedited and formatted. The final version may differ from this version. File name: Opioid peptides v45 Date: 5/28/20 Review for Mol Pharm Special Issue celebrating 50 years of INRC Five decades of research on opioid peptides: Current knowledge and unanswered questions Lloyd D. Fricker1, Elyssa B. Margolis2, Ivone Gomes3, Lakshmi A. Devi3 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; E-mail: [email protected] 2Department of Neurology, UCSF Weill Institute for Neurosciences, 675 Nelson Rising Lane, San Francisco, CA 94143, USA; E-mail: [email protected] 3Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, Annenberg Downloaded from Building, One Gustave L. Levy Place, New York, NY 10029, USA; E-mail: [email protected] Running Title: Opioid peptides molpharm.aspetjournals.org Contact info for corresponding author(s): Lloyd Fricker, Ph.D. Department of Molecular Pharmacology Albert Einstein College of Medicine 1300 Morris Park Ave Bronx, NY 10461 Office: 718-430-4225 FAX: 718-430-8922 at ASPET Journals on October 1, 2021 Email: [email protected] Footnotes: The writing of the manuscript was funded in part by NIH grants DA008863 and NS026880 (to LAD) and AA026609 (to EBM). List of nonstandard abbreviations: ACTH Adrenocorticotrophic hormone AgRP Agouti-related peptide (AgRP) α-MSH Alpha-melanocyte stimulating hormone CART Cocaine- and amphetamine-regulated transcript CLIP Corticotropin-like intermediate lobe peptide DAMGO D-Ala2, N-MePhe4, Gly-ol]-enkephalin DOR Delta opioid receptor DPDPE [D-Pen2,D- Pen5]-enkephalin KOR Kappa opioid receptor MOR Mu opioid receptor PDYN Prodynorphin PENK Proenkephalin PET Positron-emission tomography PNOC Pronociceptin POMC Proopiomelanocortin 1 Molecular Pharmacology Fast Forward. -
Wo 2009/034134 A2
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date PCT (10) International Publication Number 19 March 2009 (19.03.2009) WO 2009/034134 A2 (51) International Patent Classification: (74) Agents: MEYERS, Hans-Wilhelm et al; Patent Attor A61K 38/17 (2006.01) C07K 14147 (2006.01) neys von KreislerSelting Werner, P.O. Box 10 22 41, 50462 A61F 13/00 (2006.01) Cologne (DE). (81) Designated States (unless otherwise indicated, for every (21) International Application Number: kind of national protection available): AE, AG, AL, AM, PCT/EP2008/062067 AO, AT,AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, (22) International Filing Date: EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, 11 September 2008 (11.09.2008) IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, (25) Filing Language: English MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY,TJ, (26) Publication Language: English TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 071 16164.0 11 September 2007 (11.09.2007) EP kind of regional protection available): ARIPO (BW, GH, 08100213.1 8 January 2008 (08.01.2008) EP GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), (71) Applicant (for all designated States except US): PHARIS European (AT,BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, BIOTEC GMBH [DE/DE]; Feodor-Lynen-Strasse 31, FR, GB, GR, HR, HU, IE, IS, IT, LT,LU, LV,MC, MT, NL, 30625 Hannover (DE). -
T-4601 Rabbin Anti Α-Neoendorphin Α-Neoendorphin Is an Endogenous Opioid Neuropeptide with a Decapeptide Structure
BMA BIOMEDICALS Peninsula Laboratories T-4601 Rabbin anti α-Neoendorphin α-Neoendorphin is an endogenous opioid neuropeptide with a decapeptide structure. It is derived from the proteolytic cleavage of prodynorphin. Some studies revealed that α- Neoendorphin immunoreactive fibers throughout the human brainstem and it can be found in the caudal part of the solitary nucleus, in the caudal and the gelatinosa parts of the spinal trigeminal nucleus, and in the central grey matter of medulla. This antibody was generated by immunization of rabbits with α-Neoendorphin coupled to a carrier protein. TECHNICAL AND ANALYTICAL CHARACTERISTICS Lot number: A03648 Host species: Rabbit IgG Quantity: 400µg Format: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative. Stability: Original vial: at least one year at 4º - 8ºC from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at - 20ºC or below. Applications: This antibody has been tested and validated in ELISA against α- Neoendorphin. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user. Please see www.bma.ch for protocols and general information. Immunogen: Synthetic peptide H-Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys-OH coupled to carrier protein. BMA BIOMEDICALS, Rheinstrasse