Complete Genome Sequences of Streptomyces Spp
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Isolation, Characterization and Selection of Avermectin-Producing Streptomyces Avermitilis Strains from Soil Samples
Jundishapur J Microbiol. 2014 June; 7(6): e10366. DOI: 10.5812/jjm.10366 Research Article Published online 2014 June 1. Isolation, Characterization and Selection of Avermectin-Producing Streptomyces avermitilis Strains From Soil Samples 1,* 2 1 3 Samia Siddique ; Quratulain Syed ; Ahmad Adnan ; Fahim Ashraf Qureshi 1Department of Chemistry, Government College University, Lahore, Pakistan 2Food and Biotechnology Research Center, Pakistan Council of Scientific and Industrial Research Laboratories Complex Ferozepur, Lahore, Pakistan 3Office of Research, Innovation and Commercialization, Comsats Institute of Information and Technology, Islamabad, Pakistan *Corresponding author: Samia Siddique, Department of Chemistry, Government College University Lahore, Pakistan. Tel/ Fax: +92-3334101261, E-mail: [email protected] Received: ; Revised: ; Accepted: February 3, 2013 April 25, 2013 May 9, 2013 Background: Streptomyces avermitilis, belonging to Actinomycetes, is specialized for production of avermectin, used as an anthelmintic and insecticidal agent. It is mostly found in soil and its isolation is very crucial for medically important avermectin production. Objectives: In the present study, 10 bacterial isolates lacking antimicrobial activities were isolated from the soil samples collected from different areas of Lahore, Pakistan. Materials and Methods: Three distinctive localities of Lahore were opted for soil assortment to isolate S. avermitilis. About 50 isolates of Streptomyces species were attained through selective prescreening -
Hormaomycin, a New Peptide Lactone Antibiotic Effective in Inducing
Hormaomycin, a New Peptide Lactone Antibiotic Effective in Inducing Cytodifferentiation and Antibiotic Biosynthesis in Some Streptomyces Species Nikolaus Andres, Heinz Wolf*, and Hans Zähner Institut für Biologie II, Lehrstuhl für Mikrobiologie I der Universität, Auf der Morgenstelle 28, D-7400 Tübingen, Bundesrepublik Deutschland Z. Naturforsch. 45c, 851-855 (1990); received April 10, 1990 Streptomyces, Signal Metabolite, Hormaomycin, Cytodifferentiation, Antibiotic Overproduc tion, Antibiotic Activity Hormaomycin is a novel signal metabolite from Streptomyces griseoflavus W-384 with aerial mycelium-inducing activity. The compound has been identified as an unusual peptide lactone. Hormaomycin displays three biological activities: First, it initiates the development of aerial mycelia in some Streptomyces strains. The mechanism responsible for this activity is unknown. Secondly, hormaomycin is effective in stimulating antibiotic production in different Strepto myces species. Thus, it is possible to get overproduction of a variety of antibiotics by the use of hormaomycin in fermentation processes. Thirdly, it inhibits the growth of some bacteria. The sensitive bacteria are restricted to coryneform taxa such as Arthrobacter and Corynebacterium which are closely related to Streptomyces. Introduction tones, few other kinds of regulatory compounds Bacteria of the genus Streptomyces have evolved were investigated. B-factor, a derivative of adeno complicated differentiation mechanisms that in sine, stimulates rifamycin production in Nocardia clude not only changes in metabolism but also sp. [8], C-factor is a 34.5 kDa-protein that induces sporulation in Streptomyces griseus [9]. The changes in cellular structure. The streptomycete life cycle involves the formation of a substrate macrodiolide pamamycin stimulates aerial mycelia mycelium which, after a period of vegetative formation in Streptomyces alboniger[ 10, 11]. -
The Isolation of a Novel Streptomyces Sp. CJ13 from a Traditional Irish Folk Medicine Alkaline Grassland Soil That Inhibits Multiresistant Pathogens and Yeasts
applied sciences Article The Isolation of a Novel Streptomyces sp. CJ13 from a Traditional Irish Folk Medicine Alkaline Grassland Soil that Inhibits Multiresistant Pathogens and Yeasts Gerry A. Quinn 1,* , Alyaa M. Abdelhameed 2, Nada K. Alharbi 3, Diego Cobice 1 , Simms A. Adu 1 , Martin T. Swain 4, Helena Carla Castro 5, Paul D. Facey 6, Hamid A. Bakshi 7 , Murtaza M. Tambuwala 7 and Ibrahim M. Banat 1 1 School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland BT52 1SA, UK; [email protected] (D.C.); [email protected] (S.A.A.); [email protected] (I.M.B.) 2 Department of Biotechnology, University of Diyala, Baqubah 32001, Iraq; [email protected] 3 Department of Biology, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11568, Saudi Arabia; [email protected] 4 Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Ab-erystwyth, Wales SY23 3EE, UK; [email protected] 5 Instituto de Biologia, Rua Outeiro de São João Batista, s/nº Campus do Valonguinho, Universidade Federal Fluminense, Niterói 24210-130, Brazil; [email protected] 6 Institute of Life Science, Medical School, Swansea University, Swansea, Wales SA2 8PP, UK; [email protected] 7 School of Pharmacy and Pharmaceutical Sciences, Ulster University, Coleraine, Northern Ireland BT52 1SA, UK; [email protected] (H.A.B.); [email protected] (M.M.T.) * Correspondence: [email protected] Abstract: The World Health Organization recently stated that new sources of antibiotics are urgently Citation: Quinn, G.A.; Abdelhameed, required to stem the global spread of antibiotic resistance, especially in multiresistant Gram-negative A.M.; Alharbi, N.K.; Cobice, D.; Adu, bacteria. -
Streptomyces Cytochrome P450 Enzymes and Their Roles in the Biosynthesis of Macrolide Therapeutic Agents
Review Biomol Ther 27(2), 127-133 (2019) Streptomyces Cytochrome P450 Enzymes and Their Roles in the Biosynthesis of Macrolide Therapeutic Agents Myung-A Cho, Songhee Han, Young-Ran Lim, Vitchan Kim, Harim Kim and Donghak Kim,* Department of Biological Sciences, Konkuk University, Seoul 05025, Republic of Korea Abstract The study of the genus Streptomyces is of particular interest because it produces a wide array of clinically important bioactive molecules. The genomic sequencing of many Streptomyces species has revealed unusually large numbers of cytochrome P450 genes, which are involved in the biosynthesis of secondary metabolites. Many macrolide biosynthetic pathways are catalyzed by a series of enzymes in gene clusters including polyketide and non-ribosomal peptide synthesis. In general, Streptomyces P450 enzymes accelerate the final, post-polyketide synthesis steps to enhance the structural architecture of macrolide chemistry. In this review, we discuss the major Streptomyces P450 enzymes research focused on the biosynthetic processing of macrolide therapeutic agents, with an emphasis on their biochemical mechanisms and structural insights. Key Words: Streptomyces, P450, CYP, Biosynthesis, Macrolide, Secondary metabolite INTRODUCTION isms became important to human health with the discovery of penicillin in 1928 by Fleming, and the discovery of the anti- The phylum actinobacteria is one of the major lineages cur- tuberculosis agent streptomycin from Streptomyces griseus rently recognized within bacteria (Ventura et al., 2007). Acti- in 1944 by Waksman (Ikeda, 2017). More recently, the 2015 nobacteria are widely distributed in terrestrial, especially soil, Nobel prize in Physiology or Medicine was awarded to Omura and aquatic ecosystems (McCarthy and Williams, 1992; Stach and Campbell for their contributions to the discovery of the and Bull, 2005). -
Evaluation of Drip Applications and Foliar Sprays of the Biocontrol Product Actinovate
EVALUATION OF DRIP APPLICATIONS AND FOLIAR SPRAYS OF THE BIOCONTROL PRODUCT ACTINOVATE ON POWDERY MILDEW AND OTHER FUNGAL DISEASES OF TOMATO A Thesis presented to the Faculty of California Polytechnic State University, San Luis Obispo In Partial Fulfillment Of the Requirements for the Degree Master of Science in Agriculture with a Specialization in Soil Science by Therese Angelica Quintana-Jones June 2011 © 2011 Therese Angelica Quintana-Jones ALL RIGHTS RESERVED ii COMMITTEE MEMBERSHIP TITLE: Evaluation of Drip Applications and Foliar Sprays of the Biocontrol Product Actinovate on Powdery Mildew and Other Fungal Diseases of Tomato AUTHOR: Therese Angelica Quintana-Jones DATE SUBMITTED: June 2011 COMMITTEE CHAIR: Dr. Lynn E. Moody, Earth and Soil Sciences Department Head COMMITTEE MEMBER: Dr. Michael Yoshimura, Biological Sciences Professor COMMITTEE MEMBER: Dr. Elizabeth Will, Soil Science Lecturer iii ABSTRACT Evaluation of Drip Applications and Foliar Sprays of the Biocontrol Product Actinovate on Powdery Mildew and Other Fungal Plant Pathogens of Tomato Therese Angelica Quintana-Jones The effectiveness of the biocontrol product Actinovate® at enhancing tomato plant growth and yield, and reducing the presence of fungal pathogens was studied in greenhouse and field conditions. In the greenhouse, no differences were found among seed germination or plant survival rates, seedling heights, dry root weights, and dry shoot weights of tomato seedlings grown from seeds drenched with Actinovate® or Rootshield®. The effects of one initial Actinovate® seed drench at sowing, repeated applications through the drip irrigation throughout the season, or repeated applications through the drip irrigation plus foliar applications throughout the season at reducing plant infection by fungal plant pathogens, and increasing yield and quality for tomato plants (Solanum lycopersicum) were investigated in Los Alamos, CA, on a sandy loam soil. -
Production of an Antibiotic-Like Activity by Streptomyces Sp. COUK1 Under Different Growth Conditions Olaitan G
East Tennessee State University Digital Commons @ East Tennessee State University Electronic Theses and Dissertations Student Works 8-2014 Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions Olaitan G. Akintunde East Tennessee State University Follow this and additional works at: https://dc.etsu.edu/etd Part of the Biology Commons Recommended Citation Akintunde, Olaitan G., "Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions" (2014). Electronic Theses and Dissertations. Paper 2412. https://dc.etsu.edu/etd/2412 This Thesis - Open Access is brought to you for free and open access by the Student Works at Digital Commons @ East Tennessee State University. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Digital Commons @ East Tennessee State University. For more information, please contact [email protected]. Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions A thesis presented to the faculty of the Department of Health Sciences East Tennessee State University In partial fulfillment of the requirements for the degree Master of Science in Biology by Olaitan G. Akintunde August 2014 Dr. Bert Lampson Dr. Eric Mustain Dr. Foster Levy Keywords: Streptomyces, antibiotics, natural products, bioactive compounds ABSTRACT Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions by Olaitan Akintunde Streptomyces are known to produce a large variety of antibiotics and other bioactive compounds with remarkable industrial importance. Streptomyces sp. COUK1 was found as a contaminant on a plate in which Rhodococcus erythropolis was used as a test strain in a disk diffusion assay and produced a zone of inhibition against the cultured R. -
Genomic and Phylogenomic Insights Into the Family Streptomycetaceae Lead to Proposal of Charcoactinosporaceae Fam. Nov. and 8 No
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Genomic and phylogenomic insights into the family Streptomycetaceae 2 lead to proposal of Charcoactinosporaceae fam. nov. and 8 novel genera 3 with emended descriptions of Streptomyces calvus 4 Munusamy Madhaiyan1, †, * Venkatakrishnan Sivaraj Saravanan2, † Wah-Seng See-Too3, † 5 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 6 Singapore 117604; 2Department of Microbiology, Indira Gandhi College of Arts and Science, 7 Kathirkamam 605009, Pondicherry, India; 3Division of Genetics and Molecular Biology, 8 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 9 Malaysia 10 *Corresponding author: Temasek Life Sciences Laboratory, 1 Research Link, National 11 University of Singapore, Singapore 117604; E-mail: [email protected] 12 †All these authors have contributed equally to this work 13 Abstract 14 Streptomycetaceae is one of the oldest families within phylum Actinobacteria and it is large and 15 diverse in terms of number of described taxa. The members of the family are known for their 16 ability to produce medically important secondary metabolites and antibiotics. In this study, 17 strains showing low 16S rRNA gene similarity (<97.3 %) with other members of 18 Streptomycetaceae were identified and subjected to phylogenomic analysis using 33 orthologous 19 gene clusters (OGC) for accurate taxonomic reassignment resulted in identification of eight 20 distinct and deeply branching clades, further average amino acid identity (AAI) analysis showed 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. -
Biopesticides Fact Sheet for Streptomyces Lydicus WYEC
Streptomyces lydicus strain WYEC 108 (006327) Fact Sheet Summary Streptomyces lydicus strain WYEC 108 is a naturally occurring bacterium that is commonly found in soil. When applied to soil mixes or turf grass, the bacterium protects the plant against a range of root decay fungi. Streptomyces lydicus strain WYEC 108 can also be applied to plant foliage in greenhouses to control powdery mildew. No harm to humans or the environment is expected from use of Streptomyces lydicus strain WYEC 108 as a pesticide active ingredient. I. Description of the Active Ingredient Streptomyces lydicus strain WYEC 108 is a naturally occurring bacterium that is commonly found in soil environments. It is thought that the bacterium works by colonizing the growing root tips of plants and parasitizing root decay fungi (such as Fusarium, Pythium, and other species). The bacterium may also produce antibiotics that act against these fungi. II. Use Sites, Target Pests, And Application Methods o Use Sites: Soil mixes (for potted plants and agricultural uses), turf grass, and plant foliage in greenhouses. o Target pests: Root decay fungi such as Fusarium, Rhizoctonia, Pythium, Phytophthora, Phytomatotricum, Aphanomyces, Monosprascus, Armillaria, Sclerotinia, Postia, Verticillium, Geotrichum. Other target pests include powdery mildew and other fungal pathogens that attack plant foliage. o Application Methods: The single registered end product, “Actinovate Soluble” is mixed with water and applied as a soil mix or drench to turf grass or potted plants. The product can also be applied to plant foliage in greenhouses. III. Assessing Risks to Human Health No harmful health effects to humans are expected from use of Streptomyces lydicus strain WYEC 108 as a pesticide active ingredient. -
Gopalakrishnan Subramaniam Sathya Arumugam Vijayabharathi
Gopalakrishnan Subramaniam Sathya Arumugam Vijayabharathi Rajendran Editors Plant Growth Promoting Actinobacteria A New Avenue for Enhancing the Productivity and Soil Fertility of Grain Legumes Plant Growth Promoting Actinobacteria ThiS is a FM Blank Page Gopalakrishnan Subramaniam • Sathya Arumugam • Vijayabharathi Rajendran Editors Plant Growth Promoting Actinobacteria A New Avenue for Enhancing the Productivity and Soil Fertility of Grain Legumes Editors Gopalakrishnan Subramaniam Sathya Arumugam Bio-Control, Grain Legumes Bio-Control, Grain Legumes ICRISAT, Patancheru, Hyderabad ICRISAT, Patancheru, Hyderabad Telangana, India Telangana, India Vijayabharathi Rajendran Bio-Control, Grain Legumes ICRISAT, Patancheru, Hyderabad Telangana, India ISBN 978-981-10-0705-7 ISBN 978-981-10-0707-1 (eBook) DOI 10.1007/978-981-10-0707-1 Library of Congress Control Number: 2016939389 # Springer Science+Business Media Singapore 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. -
Bioactive Molecules from Mangrove Streptomyces Qinglanensis 172205
marine drugs Article Bioactive Molecules from Mangrove Streptomyces qinglanensis 172205 Dongbo Xu 1, Erli Tian 1, Fandong Kong 2 and Kui Hong 1,* 1 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; [email protected] (D.X.); [email protected] (E.T.) 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultura Sciences, Haikou 571101, China; [email protected] * Correspondence: [email protected]; Tel.: +86-27-6875-2442 Received: 17 April 2020; Accepted: 12 May 2020; Published: 13 May 2020 Abstract: Five new compounds 15R-17,18-dehydroxantholipin (1), (3E,5E,7E)-3-methyldeca-3,5,7- triene-2,9-dione (2) and qinlactone A–C (3–5) were identified from mangrove Streptomyces qinglanensis 172205 with “genetic dereplication,” which deleted the highly expressed secondary metabolite-enterocin biosynthetic gene cluster. The chemical structures were established by spectroscopic methods, and the absolute configurations were determined by electronic circular dichroism (ECD). Compound 1 exhibited strong anti-microbial and antiproliferative bioactivities, while compounds 2–4 showed weak antiproliferative activities. Keywords: mangrove Streptomyces; genetic dereplication; anti-microbial; antiproliferative 1. Introduction Microbial natural products are an important source of drug lead. Mangrove streptomycetes were reported as a potential source of plenty of antiproliferative or anti-microbial chemicals with novel structures [1]. The bioinformatics of easily available genome information from microorganisms breaks the bottleneck of traditional natural product discovery to a certain extent, and secondary metabolites isolation guided by genome sequence has increasingly become a research frontier [2]. Genome mining and silent gene cluster activation unveil the potential of diverse secondary metabolites in bacteria [3–5]. -
Differential Gene Expression Analysis of Aclacinomycin Producing Streptomyces Galilaeus ATCC 31615 and Its Mutant
Differential gene expression analysis of aclacinomycin producing Streptomyces galilaeus ATCC 31615 and its mutant Master’s thesis MD A B M Sharifuzzaman Department of Life Technologies Molecular Systems Biology University of Turku Supervisor: Co-supervisor: Professor Mikko Metsä-Ketelä, Ph.D. Keith Yamada, M.Sc. Department of Life Technologies PhD candidate Antibiotic Biosynthesis Engineering Lab Department of Life Technologies University of Turku Antibiotic Biosynthesis Engineering Lab University of Turku Master’s thesis 2021 The originality of this thesis has been checked in accordance with the University of Turku Quality assurance system using the Turnitin Originality check service Summary Streptomyces from the genus Actinomycetales are soil bacteria known to have a complex secondary metabolism that is extensively regulated by environmental and genetic factors. Consequently they produce antibiotics that are unnecessary for their growth but are used as a defense mechanism to dispel cohabiting microorganisms. In addition to other isoforms Streptomyces galilaeus ATCC 31615 (WT) produces aclacinomycin A (Acl A), whereas its mutant strain HO42 (MT) is an overproducer of Acl B. Acl A is an anthracycline clinically approved for cancer chemotherapy and used in Japan and China. A better understanding of the how the different isoforms of Acl are made and investigations into a possible Acl recycling system would allow us to use metabolic engineering for the generation of a strain that produces higher quantities of Acl A with a clean production profile. In this study, RNA-Seq data from the WT, and MT strains on the 1st (D1), 2nd (D2), 3rd (D3), and 4th (D4) day of their growth was used and differentially expressed genes (DEGs) were identified. -
Biopesticides Fact Sheet for Streptomyces Griseoviridis
Streptomyces griseoviridis Strain K61 (129069) Fact Sheet Summary Streptomyces griseoviridis Strain K61 is a soil bacterium that can prevent certain disease-causing fungi from infecting plants. It is not expected to harm humans, other non-target organisms, or the environment. I. Description of the Active Ingredient Streptomyces griseoviridis Strain K61 is a naturally occurring soil bacterium initially isolated from peat in Finland. It seems to act against disease-causing fungi in at least two ways. By colonizing plant roots before the disease organisms get there, it deprives them of space and nourishment. It also produces several kinds of chemicals that may attack the harmful fungi. (A closely related bacterial species produces the antibiotic streptomycin.) II. Use Sites, Target Pests, And Application Methods o Use Sites: Certain plants in containers, greenhouses, or in the field. o Target pests: Specific kinds of fungi that cause seed rot, root and stem rot, wilt, and damping off diseases in various food crops, ornamentals, and tree seedlings. o Application Methods: Depending on the plant and other circumstances, the active ingredient can be applied to seeds, to the soil, to roots, to transplants, or as a dip or spray. Because it acts to prevent fungal diseases, rather than to treat them, the active ingredient should be applied before the disease organisms gain a foothold. III. Assessing Risks to Human Health Based on results of various studies, Streptomyces griseoviridis Strain K61 is not expected to infect humans or to cause harmful effects if ingested. However, users of pesticide products with this active ingredient may experience mild eye and skin irritation, as well as adverse effects on lungs.