DOCSLIB.ORG

COXJR-DISSERTATION-2018.Pdf (3.449Mb)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
COXJR-DISSERTATION-2018.Pdf (3.449Mb) XANTHOMONAS LIVING THE SWEET LIFE: CHARACTERIZATION OF A PLANT SUGAR TRANSPORTER FAMILY AND ITS ROLE IN BACTERIAL BLIGHT OF COTTON SUSCEPTIBILITY A Dissertation by KEVIN LAMARR COX JR. Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Chair of Committee, Libo Shan Committee Members, Michael Kolomiets Ping He Tom Isakeit Head of Department, Leland S. Pierson December 2018 Major Subject: Plant Pathology Copyright 2018 Kevin Lamarr Cox Jr. ABSTRACT Upland cotton (Gossypium hirsutum) is an important cash crop that is constantly pressured by various biotic stresses. Bacterial blight of cotton (BBC) caused by Xanthomonas citri subsp. malvacearum (Xcm) is a destructive disease that limits cotton production worldwide. Xcm causes disease by injecting transcription activator-like (TAL) effectors in plant cell to directly induce the expression of host genes. Despite having at least 10 TAL effectors cloned from Xcm, there have yet to be any susceptibility or resistance proteins identified and characterized. The rapid re-emergence of this disease in the last few years in the US calls for pressing needs to decipher the molecular mechanisms of BBC in order to provide resources to combat this pathogen. In this dissertation study, I identified a plant sugar transporter (GhSWEET) gene family in cotton that plays an important role in BBC susceptibility. To bypass the traditional map-based cloning to identify susceptibility targets, a combination of genome-wide transcriptome profiling and computational prediction of TAL effector binding sites, coupled with functional characterization of candidate genes was used to identify GhSWEET10 as a key susceptibility target of Avrb6, a major TAL effector in the strain of XcmH1005. This was the first reported susceptibility gene in cotton. Additionally, my data suggest that activating different GhSWEETs by a repertoire of TAL effectors is a common, but important, strategy for Xcm to cause infection, as current Xcm field isolates activated two different GhSWEETs, GhSWEET14a and GhSWEET14b. Further functional analysis on these two SWEETs revealed that activating ii expression of GhSWEET14a and GhSWEET14b was correlated with disease susceptibility and genetically required for Xcm to cause infection on cotton. Finally, by using genome editing and artificial microRNAs, I was able to develop strategies that could potentially be used to biologically control of BBC. In conclusion, my dissertation revealed the molecular mechanism of BBC and provided a set of toolkits that could be used in the future to develop BBC-tolerant cotton cultivars. iii DEDICATION I dedicate this dissertation to my lovely wife, Charneva Cox, for motivating me to go to graduate school, showing patience, and being my number one supporter, and my daughter, Kyra Cox, for allowing me to interrupt our fun activities at times in order to work in the lab. I also dedicate this dissertation to my father and mother, Kevin Sr. and Gloria Cox, and to my brother, Christopher Cox, for their encouragement and support. Finally, I dedicate this dissertation to my extended family and friends that gave me their support throughout my degree program. iv ACKNOWLEDGEMENTS I would like to sincerely thank my committee chair, Dr. Libo Shan, and her collaborator, Dr. Ping He, for allowing me to conduct my Ph.D. studies in their labs. I’m deeply grateful for their patience, constructive criticisms, and guidance during my time as a graduate student. Their mentoring had a dramatic impact on my development as a scientist. The experiences and opportunities I have encountered would not have been possible without the two of them. I deeply appreciate for all they have done for me and I look forward to applying the mentoring I’ve received from them to build my career. I would like to thank my committee members, Dr. Mike Kolomiets and Dr. Tom Isakeit, for their guidance and support throughout the course of this research. I appreciated the interactions and scientific discussions with them, as they provided valuable inputs to significantly improve my research. I greatly appreciate the collaboration of my work with Dr. Adam Bogdanove and members of his lab. This collaboration has been on-going for nearly my entire tenure as a graduate student. It has been nothing but fruitful and rewarding, as he and his lab conducted key experiments and provided useful inputs to help my projects. I couldn’t have some experiments in a timely manner without them. Many thanks to the current and previous lab members of Dr. Shan’s and Dr. He’s lab that I’ve worked with during my graduate studies. They provided great mentoring and scientific discussions during my time in the lab. In addition, they provided fun memories that I will treasure forever. I would like to give a special thanks to Dr. v Fanhong Meng, who collaborated with me on the work in Chapter 2 and provided valuable mentoring that allowed me to significantly improve my lab techniques. Additional thanks to Dr. Fangjun Li, Dr. Ping Wang, and Dr. Xiyu Ma for their help on various experiments presented in this work. Thanks to the students in the PLPM department for making my graduate studies here enjoyable. I will never forget the times we had together and there are bonds that I have made with some of you that I will cherish forever. I would also like to thank the faculty and staff in the PLPM department, as well as those members of the Institute for Plant Genomics and Biotechnology Center for their helpful interactions. Many thanks to my friends that I made outside the PLPM department for their fun times and memories. Finally, thanks to my mom, dad, and brother for their continued encouragement and love, my extended family for their support, and to my wife and daughter for their patience and their love. I’m deeply grateful for all of them. vi CONTRIBUTORS AND FUNDING SOURCES Contributors This work was supervised by a dissertation committee consisting of Professor Libo Shan [advisor], Professors Michael Kolomiets and Tom Isakeit of the Department of Plant Pathology & Microbiology, and Professor Ping He of the Department of Biochemistry & Biophysics. The work in Chapter 2 was performed in collaboration with Dr. Adam Bogdanove’s lab of Cornell University and contributed by the authors listed in the publication. The XcmH strains and plasmids were contributed by Dr. Dean Gabriel of the University of Florida. The crop transformation in Chapter 3 was performed by Mr. Marco and Mrs. Mayra Molina of the Laboratory of Crop Transformation located in the Institute of Plant Genomics and Biotechnology Center. All other work conducted for the dissertation was completed by the student independently. Funding Sources Graduate study was supported by a Graduate Diversity Fellowship from Texas A&M University and by a USDA-NIFA AFRI Pre-doctoral Fellowship under Grant Number 2017-67011-26060. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of USDA-NIFA. vii TABLE OF CONTENTS Page ABSTRACT .......................................................................................................................ii DEDICATION .................................................................................................................. iv ACKNOWLEDGEMENTS ............................................................................................... v CONTRIBUTORS AND FUNDING SOURCES ............................................................vii TABLE OF CONTENTS ............................................................................................... viii LIST OF FIGURES ........................................................................................................... xi LIST OF TABLES ...........................................................................................................xii CHAPTER I INTRODUCTION AND LITERATURE REVIEW: RETURN OF AN OLD FOE: RE-EMERGENCE OF BACTERIAL BLIGHT OF COTTON ...................... 1 Overview ........................................................................................................................ 1 Introduction .................................................................................................................... 1 Bacterial blight of cotton ................................................................................................ 2 Targeting veins to hijack the water source of plants ...................................................... 7 Increase in humidity and temperature plays a role in disease severity .......................... 9 Molecular strategies to breed resistant cultivars .......................................................... 10 Conclusions .................................................................................................................. 11 CHAPTER II TAL EFFECTOR DRIVEN INDUCTION OF A SWEET GENE CONFERS SUSCEPTIBILITY TO BACTERIAL BLIGHT OF COTTON ................... 13 Overview ...................................................................................................................... 13 Introduction .................................................................................................................. 14 Results .......................................................................................................................... 17 Genome and TAL effector sequences of Xcm H1005 and N1003 ........................... 17 Candidate
Load more

Recommended publications
  • The Roles of TAL Effectors in Nature in Relation to Their Unique Properties As DNA-Targeting Tools
    The Roles of TAL Effectors in Nature in Relation to their Unique Properties as DNA-Targeting Tools Adam Bogdanove Cornell University Ithaca, New York [email protected] I will present perspectives on the unique properties of TAL effectors as DNA-targeting tools, and what we can gain from understanding how they work in nature and in plant disease. I’ll begin by comparing and contrasting the CRISPR/Cas9 and TAL-effector nuclease (TALEN) systems to highlight some of the unique features of the latter (Figure 1). TAL-effector nucleases work as dimer proteins. Each individual monomer of the TAL-ef- fector domain is about 102 kDa. Targets are encoded by repeats in each protein. In contrast with CRISPRs, typically you need one dimer per target, so it’s not readily multiplexed, certainly not as readily as the CRISPR/Cas9. The targeting specificity is typically 15 to 20 bases times 2. Therefore, on the surface, in these base configurations, TAL effectors inherently have great specificity, but, as we heard from Dan Voytas1 and Ann Ran2, a number of tricks have been applied within the CRISPR/Cas9 architecture to improve their specificity. So, they are complementary, effective systems. 1Pages 29–37. 2Pages 69–81. 3 Figure 1. Comparison of DNA-targeting systems. (Images adopted from van der Oost, 2013) TALEN Utility I was interested to see what would happen in the genome-editing field as CRISPRs gained momentum with possibly less emphasis on TAL effectors. However, a proliferation of TALEN kits have been deposited with Addgene3—which Dan Voytas4 and I are familiar with because we deposited there—and over 1,400 requests have been filled for our kit.
    [Show full text]
  • Humankind 2.0: the Technologies of the Future 6. Biotech
    Humankind 2.0: The Technologies of the Future 6. Biotech Piero Scaruffi, 2017 See http://www.scaruffi.com/singular/human20.html for the full text of this discussion A brief History of Biotech 1953: Discovery of the structure of the DNA 2 A brief History of Biotech 1969: Jon Beckwith isolates a gene 1973: Stanley Cohen and Herbert Boyer create the first recombinant DNA organism 1974: Waclaw Szybalski coins the term "synthetic biology” 1975: Paul Berg organizes the Asilomar conference on recombinant DNA 3 A brief History of Biotech 1976: Genentech is founded 1977: Fred Sanger invents a method for rapid DNA sequencing and publishes the first full DNA genome of a living being Janet Rossant creates a chimera combining two mice species 1980: Genentech’s IPO, first biotech IPO 4 A brief History of Biotech 1982: The first biotech drug, Humulin, is approved for sale (Eli Lilly + Genentech) 1983: Kary Mullis invents the polymerase chain reaction (PCR) for copying genes 1986: Leroy Hood invents a way to automate gene sequencing 1986: Mario Capecchi performs gene editing on a mouse 1990: William French Anderson’s gene therapy 1990: First baby born via PGD (Alan Handyside’s lab) 5 A brief History of Biotech 1994: FlavrSavr Tomato 1994: Maria Jasin’s homing endonucleases for genome editing 1996: Srinivasan Chandrasegaran’s ZFN method for genome editing 1996: Ian Wilmut clones the first mammal, the sheep Dolly 1997: Dennis Lo detects fetal DNA in the mother’s blood 2000: George Davey Smith introduces Mendelian randomization 6 A brief History of Biotech
    [Show full text]
  • Symposium 2014 Community
    Participation: Contact Information: Interdepartmental Graduate Program in The symposium is open to MSU faculty, staff, Dr. Guo-Qing Song Plant Breeding, Genetics & Biotechnology graduate students and undergraduates, as well Faculty Advisor as members of neighboring institutions and the [email protected] Symposium 2014 community. There is no registration fee or 355-5191 (Ex. 1384) requirement for preregistration. Dr. David Douches Faculty Advisor Director - Plant Breeding, Genetics and Poster Session: Biotechnology Program A poster session and open reception for the [email protected] speakers will immediately follow the talks in the 355-0271 ext. 1198 MPS atrium. Light refreshments will be served. Schedule: Those who wish to present a research poster 9:00 – 9:10 (4'X 4') are invited to do so. Graduate students Welcome – Dave Douches – Director PBGB and faculty associated with PBGB are particularly encouraged to participate. Please 9:10 – 10:00 register for the poster session by emailing Dr. Dr. Adam Bogdanove Dave Douches. Cornell University – Plant Pathology “The roles of TAL effectors in nature in relation to their unique properties as DNA targeting tools” Luncheon: 10:00 – 10:30 Plant Breeding, Genetics, and Biotechnology Break and Genetics Program graduate students and 10:40 – 11:30 faculty who would like to participate in the Dr. Gary Rudgers luncheon with the speakers please RSVP by DOW AgroScience – Global Regulatory Leader email to Dr. Dave Douches at: “Plant Genome Editing: Biotech Tools for the Next [email protected] Generation ” 11:30 – 1:30 Sponsorship Lunch The PBGB program wishes to acknowledge support for the symposium from Dow 1:30 – 2:25 AgroSciences and MSU AgBioResearch.
    [Show full text]
  • Filed on Behalf of Junior Party Paper No
    Filed on behalf of Junior Party Paper No. ___ THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNIVERSITY OF VIENNA, AND EMMANUELLE CHARPENTIER By: Eldora L. Ellison, Ph.D., Esq. By: Li-Hsien Rin-Laures, M.D., Esq. Eric K. Steffe, Esq. RINLAURES LLC David H. Holman, Ph.D., Esq. 321 N. Clark Street, 5th floor Byron L. Pickard, Esq. Chicago, IL 60654 John Christopher Rozendaal, Esq. Tel. (773) 387-3200; Fax (773) 929-2391 Paul A. Ainsworth, Esq. [email protected] Michael E. Joffre, Ph.D., Esq. STERNE, KESSLER, GOLDSTEIN & FOX P.L.L.C. Sandip H. Patel, Esq. 1100 New York Avenue, NW MARSHALL GERSTEIN & BORUN LLP Washington, D.C. 20005 6300 Willis Tower, 233 South Wacker Tel: (202) 371-2600 Drive Fax: (202) 371-2540 Chicago, IL 60606 [email protected] Tel: (312) 474-6300; Fax: (312) 474-0448 [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] UNITED STATES PATENT AND TRADEMARK OFFICE BEFORE THE PATENT TRIAL AND APPEAL BOARD THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNIVERSITY OF VIENNA, AND EMMANUELLE CHARPENTIER Junior Party (Applications 15/947,680; 15/947,700; 15/947,718; 15/981,807; 15/981,808; 15/981,809; 16/136,159; 16/136,165; 16/136,168; 16/136,175; 16/276,361; 16/276,365; 16/276,368; and 16/276,374), v. THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, and PRESIDENT AND FELLOWS OF HARVARD COLLEGE Senior Party (Patents 8,697,359; 8,771,945; 8,795,965; 8,865,406; 8,871,445; 8,889,356; 8,895,308; 8,906,616; 8,932,814; 8,945,839; 8,993,233; 8,999,641; 9,840,713; and Application 14/704,551).
    [Show full text]
  • Ferdinand Hucho, Julia Diekämper, Heiner Fangerau, Boris Fehse
    Ferdinand Hucho, Julia Diekämper, Heiner Fangerau, Boris Fehse, Jürgen Hampel, Kristian Köchy, Sabine Könninger, Lilian Marx-Stölting, Bernd Müller-Röber, Jens Reich, Hannah Schickl, Jochen Taupitz, Jörn Walter, Martin Zenke (Hrsg.) Martin Korte (Sprecher) Vierter Gentechnologiebericht Bilanzierung einer Hochtechnologie Baden-Baden: Nomos, 2018 ISBN: 978-3-8487-5183-9 (Forschungsberichte / Interdisziplinäre Arbeitsgruppen der Berlin-Brandenburgischen Akademie der Wissenschaften ; 40) Persistent Identifier: urn:nbn:de:kobv:b4-opus4-30102 Die vorliegende Datei wird Ihnen von der Berlin-Brandenburgischen Akademie der Wissenschaften unter einer Creative Commons Attribution-NonCommercial-NoDerivateWorks 4.0 International (cc by-nc-nd 4.0) Licence zur Verfügung gestellt. Forschungsberichte der interdisziplinären Arbeitsgruppen der Berlin-Brandenburgischen Akademie der Wissenschaften Vierter Gentechnologiebericht Bilanzierung einer Hochtechnologie Herausgegeben von Ferdinand Hucho | Julia Diekämper | Heiner Fangerau | Boris Fehse Jürgen Hampel | Kristian Köchy | Sabine Könninger | Lilian Marx-Stölting Bernd Müller-Röber | Jens Reich | Hannah Schickl | Jochen Taupitz Jörn Walter | Martin Zenke | Martin Korte (Sprecher) Nomos U1_4. GTB_BBAW_5183-9.indd 1 12.09.18 13:58 Forschungsberichte der interdisziplinären Arbeitsgruppen der Berlin-Brandenburgischen Akademie der Wissenschaften Vierter Gentechnologiebericht Bilanzierung einer Hochtechnologie Herausgegeben von Ferdinand Hucho | Julia Diekämper | Heiner Fangerau | Boris Fehse Jürgen Hampel |
    [Show full text]
  • Perspectives in Plant Biology
    Interdepartmental Plant Biology Major Fall Seminar Series (P Phy 696) Perspectives in Plant Biology Wednesdays 4:10 – 5:30 pm Fall, 2008 210 Bessey Date Speaker Topic August 27 Stephanie Moon (IPB, BBMB, VP) • Issues regarding P Phy 696 Jen Gray (IPB, BBMB, VP) • Fall Retreat Kan Wang (Agron, IPB Chair) September 3 Tracie Hennen-Bierwagen Biochemical and genetic analyses of physical (BBMB, IPB) associations among Zea mays starch biosynthetic enzymes September 17 Adam Bogdanove Pathogen manipulation of host gene expression (Plant Path) in bacterial diseases of rice September 24 Erik Vollbrecht Cell biological insights from Maize (GDCB) developmental mutants October 1 Jianbo Zhang / Tom Peterson Transposon-Induced Genome Rearrangements (GDCB) in Maize October 22 Olga Zabotina What do we know about plant cell walls and (BBMB) how important is it? October 29 Kirk Maloney Invasion biology using a geo-ecological (EEOB) approach November 12 Frederica Brandizzi Dynamics of the early plant secretory pathway (Michigan State University) November 19 Jack Horner The Role of Microscopy in Plant Cell Biology (GDCB) December 3 Stephanie Moon (IPB, BBMB, VP) • Fall P Phy 696 conclusion Jen Gray (IPB, BBMB, VP) • Arrange spring seminar series Kan Wang (Agron, IPB Chair) • Elect new officers for the IPPM Graduate Student Organization Light refreshments will be available prior to seminar Direct questions or comments to Stephanie Moon at 4-0347/ [email protected] or Jen Gray at 4- 0347 /[email protected] Plant Biology Fall Seminar Series (P Phy 696) Perspective in Plant Biology Wednesdays 4:10 – 5:30 pm 210 Bessey Hall Invited Speaker: Dr. Tracie Hennen-Bierwagen (BBMB) September 3, 2008 Biochemical and genetic analyses of multimeric complexes containing starch biosynthetic enzymes in maize endosperm Abstract Starch is assembled by the combined actions of multiple starch synthases (SS), starch branching enzymes (SBE), and starch debranching enzymes, however, the mechanisms responsible determining the molecular architecture of the polymer are unknown.
    [Show full text]
  • Download Friday Notes
    Friday Notes is designed to enhance communication among various agricultural sectors, educators, students, and the public who are interested in a variety of plant, animal, food, and environmental issues. Friday Notes advocates the pursuit of credible, unbiased, science- based information. Material contained in linked articles is from the original authors and does not necessarily reflect the views of CAST. July 26, 2019 In This Issue...... Click to Read Gene Editing and the Public Impact Reports for Gene Editing and Free-Range Poultry Papers--P. 2 CRISPR and related techniques may be "everywhere," but many in the Animal Agriculture News general public are still unsure about the procedures and the significance. Food Science and Safety News Gene editing has been in the news Plant and Environment News for decades, with headlines touting everything from beneficial medical International News advances to suspicious Brave New General Interest News World allusions. Scientists have generally been positive about the methods, with many possible Drone Views agriculture and food production applications in the works. A CAST Commentary in 2011 focused on the topic of genetically engineered animals, and the organization has used publications, social media, and Friday Notes links to help inform readers (see the first link in the "News" section below, the genome editing publication impact report on page two below, and various links in this newsletter). Here are a few of the many recent releases about gene editing: (1) This site provides various links related to CRISPRcon 2019. (2) A This year's Drone Awards for new paper from Europe explores how scientists can use CRISPR to photography include shots of a enhance traditional plant breeding techniques with the goal of ensuring mesmerizing ancient town in Algeria global food security.
    [Show full text]
  • Download Friday Notes
    Having trouble viewing this email? Click here Friday Notes is designed to enhance communication among various agricultural sectors, educators, students, and the public who are interested in a variety of plant, animal, food, and environmental issues. Friday Notes advocates the pursuit of credible, unbiased, science- based information. Material contained in linked articles is from the original authors and does not necessarily reflect the views of CAST. In This Issue...... Click to Read July 13, 2018 New Genome Editing Publication Rolls Out--P. 2 Genome Editing in Agriculture CAST publication adds to this important dialogue Animal Agriculture News Food Science and Safety News Dr. Adam Bogdanove (at right) of Cornell University led three presentations of CAST's Plant and Environment News newest publication, Genome Editing in Agriculture: Methods, Applications, and International News Governance on July 9 in Washington, D.C. General Interest News The topic is timely, the technology is quickly expanding, and the effects are certain to be Upcoming Poultry wide ranging. CAST is a part of the effort to Publication Rollout on make this breakthrough more accessible for July 18 in D.C. the public and policymakers. Jennifer Doudna, a CRISPR pioneer, says that public understanding of gene editing is catching up through a deluge of media coverage. "This increased fascination has led to a greater thirst to realize the benefits of CRISPR's applications," she says. CAST's new publication is more than media coverage--it looks at the science, the benefits, and the governance of genome editing. The authors focus their attention on a tool that can increase the positive impacts of plant and animal breeding on human welfare and sustainability.
    [Show full text]
  • Martin BTI CV 2015
    Curriculum Vitae November 2015 GREGORY B. MARTIN Professor Section of Plant Pathology and Plant-Microbe Biology School of Integrative Plant Science Email: [email protected] Cornell University Tel.: (607) 254-1208 Ithaca, NY 14853 Fax: (607) 254-1242 Boyce Schulze Downey Professor Boyce Thompson Institute for Plant Research Ithaca, NY 14853 RESEARCH & TEACHING EXPERTISE Molecular basis of bacterial infection processes – Molecular basis of plant immunity and disease susceptibility – Application of genomics approaches to gene discovery in bacterial pathogens and plants EDUCATION 1989 – 1992 Postdoctoral Fellow Cornell University 1989 Ph.D. Genetics Michigan State University 1984 M.S. Plant Breeding Michigan State University 1979 B.S. Crop & Soil Science Michigan State University ACADEMIC RANKS 2005 – Present Boyce Schulze Downey Professor, Boyce Thompson Institute for Plant Research 1998 – Present Professor, Department of Plant Pathology and Plant-Microbe Biology, Cornell University 2014 Acting President, Boyce Thompson Institute for Plant Research 2011 – 2014 Honorary Distinguished Professor of Biological Sciences, King Abdulaziz University, Saudi Arabia 1998 – 2005 Professor, Boyce Thompson Institute for Plant Research 1996 – 1998 Associate Professor, Department of Agronomy, Purdue University 1992 – 1996 Assistant Professor, Department of Agronomy, Purdue University PROFESSIONAL EXPERIENCE 1989 – 1992 NSF Postdoctoral Fellow, Department of Plant Breeding & Genetics, Cornell University 1985 – 1989 Graduate Fellow, Genetics Program, DOE Plant
    [Show full text]
  • Genome Editing in Agriculture
    Council for Agricultural Science and Technology Kent G. Schescke Executive Vice President 2018 The CAST Mission The CAST Vision A world where decision making related to agriculture and natural resources is based on credible information developed through reason, science, and consensus building. What Does CAST Do? • CAST disseminates science- based information through: o Print materials o Online sources o Videos on website, YouTube, and SchoolTube o Spanish and Chinese translations of select publications How Does CAST Do This? With the help of many volunteer contributors: • 50 Board Members representing scientific societies, companies, nonprofits, and 65% academia universities 15% government • 160+ active task force members working on 15% companies CAST reports yet to be released 5% nonprofits • Volunteer scientific experts as authors and reviewers—more than 700 volunteers since 2007 Unique Formats for Unique Needs • Issue Papers. 8-20 pages; more narrowly focused. • Special Publications. 25-150 pages; often follow-on to workshop. • Task Force Reports. 40-200 pages; comprehensive overview. • Commentaries. 4-8 pages; quick turnaround. • Ag QuickCASTs. 1-page excerpts from full CAST publications. Other Forthcoming Publications • Impact of Free-Range Poultry Production Systems on Animal Health, Human Health, Productivity, Environment, Food Safety, and Animal Welfare Issues [July 2018 rollout] • Food Loss and Waste [Summer 2018 rollout] Friday Notes •Published 48 times annually •Lead articles on timely ag topics •Dozens of ag news briefs from 100+ sources with live links to original articles •An international news section •Legislative updates from D.C. CAST Welcomes Your Suggestions CAST welcomes suggestions, ideas, and recommendations for future CAST publications and projects. Anyone—whether a CAST member or nonmember—may submit a proposal for consideration, using the CAST Proposal Form.
    [Show full text]
  • Computational and Experimental Analysis of TAL Effector-DNA Binding Erin Lynn Doyle Iowa State University
    Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2013 Computational and experimental analysis of TAL effector-DNA binding Erin Lynn Doyle Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Agricultural Science Commons, Agriculture Commons, Bioinformatics Commons, Biology Commons, and the Plant Pathology Commons Recommended Citation Doyle, Erin Lynn, "Computational and experimental analysis of TAL effector-DNA binding" (2013). Graduate Theses and Dissertations. 13132. https://lib.dr.iastate.edu/etd/13132 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Computational and experimental analysis of TAL effector-DNA binding by Erin Lynn Doyle A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Bioinformatics and Computational Biology Program of Study Committee: Adam J. Bogdanove, Co-Major Professor Daniel Nettleton, Co-Major Professor Susan Lamont Leonor Leandro Steve Whitham Iowa State University Ames, Iowa 2013 Copyright © Erin Lynn Doyle, 2013. All rights reserved. ii TABLE OF CONTENTS LIST OF FIGURES ..................................................................................................................
    [Show full text]