Directory of Suppliers

The directory lists the major suppliers of instruments and acces­ sories. A more complete list can be found in J. Gas Chromatog., June 1965. Suppliers are generally listed under one heading only although many of the large instrument manufacturers supply most of the listed accessories and supplies.

INSTRUMENTS AMERICAN INSTRUMENT CO., INC. MICRO-TEK INSTRUMENTS, INC. 8030 Georgia Avenue P. O. Box 15409 Silver Spring, Maryland 20910 Baton Rouge. Louisiana

BARBER-COLMAN COMPANY NESTER/FAUST Rockford. Illinois 2401 Ogletown Road Newark, Delaware BECKMAN INSTRUMENTS. INC. 2500 Harbor Boulevard PACKARD INSTRUMENT CO .. INC. Fullerton. California 92634 2200 Warrenville Road Downers Grove, Illinois 60515 BURRELL CORPORATION 2223 Fifth Avenue THE PERKIN-ELMER CORP. Pittsburgh 19, Pennsylvania 870 Main Avenue Norwalk, Connecticut CARY INSTRUMENTS Applied Physics Corporation 2724 South Peck Road WARNER-CHILCOTT LABORATORIES Monrovia, California Instrument Division 200 South Garrard Boulevard F & M SCIENTIFIC CORP. Richmond, California Route 41 and Starr Road Avondale, Pennsyl vania WILKENS INSTRUMENT & RESEARCH INC. 2700 Mitchell Drive, Box 313 GLOWALL CORPORATION Walnut Creek. California 2530 Wyandotte Road Willow Grove, Pennsylvania BECKER-DELFT (Julian H. Becker N.V.) JARRELL-ASH COMPANY Vulcanusweg 113, P. O. Box 219 590 Lincoln Street Delft, Holland Waltham, Massachusetts 02154 BODENSEEWERK PERKIN ELMER & CO. LOENCO, INC. GmbH 2092 North Lincoln Avenue 777 Uberlingen/Bodensee Altadena, California 91002 West Germany 267 268 Directory of Suppliers

CARLO ERBA S.p.A. JOHNS-MANVILLE PRODUCTS CORP. Via Carlo Imbonati, 24 Celite Division Milano, Italy 22 East 40th Street New York, New York GAS CHROMATOGRAPHY LIMITED Boyn Valley Road, Maidenhead MAY & BAKER LIMITED Berkshire, England Dagenham Essex, England

W. G. PYE & CO. LTD. MICROBEADS DIVISION P. O. Box 60, York Street Cataphote Corporation Cambridge, England P. O. Box 2369 J ackson, Mississippi SClENTIFIC KIT CO. P. O. Box 244 SCHLESINGER CHEMICAL CORP. Washington, Pennsylvania 580 Mineola Avenue Carle Place, L.I .. New York

SHANDON SCIENTIFIC COMPANY, LTD. 6 Cromwell PI ace DETECTORS London SW 7, England CARLE INSTRUMENTS, INC. 532 South Rose Street SIEMENS & HALSKE AKTIENGESELL- Anaheim, California 92805 SCHAFT Wernerwerk fur Messtechnik GOW-MAC INSTRUMENT COMPANY Rheinbruckenstrasse 50, P .O.B. 4480 100 Kings Road 75 Karlsruhe, West Germany Madison, New Jersey 07940 (Thermal conductivity, gas density) DR. VIRUS KG Luftelberger Strasse IONICS RESEARCH, INC. Meckenheim bei Bonn, Germany 22 Sandalwood Drive Houston, Texas 77024 COLUMN SUPPORT MATERIALS (Ionization detectors) AND STATIONARY PHASES PACKARD INSTRUMENT CO., INC. 2200 Warrenville Road ANALABS,INCORPORATED Downers Grove, Illinois 60515 P. O. Box 5215 (Radioisotope detectors) Hamden, Connecticut 06518 RECORDERS APPLIED SClENCE LABORATORIES, INC. P. O. Box 140 THE BRISTOL COMP ANY 140 North Barnard Street Waterbury 20, Connecticut State College, Pennsylvania ESTERLINE ANGUS INSTRUMENT CO., THE BRITISH DRUG HOUSES LTD. INC. THE EALING CORPORA TlON Box 596 225 Massachusetts Avenue Indianapolis, Indiana 46206 Cambridge, Massachusetts 02140 HONEYWELL,INCORPORATED COAST ENGINEERING LABORATORY Wayne and Windrim Avenues 3755 Inglewood Avenue Philadelphia 44, Pennsylvania Redondo Beach, California (Honeywell Controls, Ltd., England)

W. H. CURTIN & COMPANY GEORGE KENT LIMITED 1800 Sidney Street Luton, Bedfordshire Houston 1. Texas England

THE EAGLE-PICHER COMPANY LEEDS & NORTHRUP CO. American Building 4901 Stenton Ave. Cincinnati I, Ohio Philadelphia 44, Pennsylvania Directory of SuppIiers 269

E. H. SARGENT & COMPANY PIPE AND TUBE FITTINGS 4647 West Foster Avenue CAJON COMPANY Chicago 30, Illinois 3550 01d South Miles Road Solon 39, Ohio TEXAS INSTRUMENTS, INC. 3609 Buffalo Speedway CRAWFORD FITTING COMP ANY P. O. Box 66027 884 East 140th Street Houston 6, Texas Cleveland 10, Ohio

VARIAN ASSOCIATES HOKE INCORPORATED Recorder Division I TenakilJ Park 611 Hansen Way CresskilJ, New Jersey Pal0 Alto, California 94303 P ARKER-HANNIFIN CORPORATION WESTRONICS,INCORPORATED 17325 Euclid Avenue 3605 McCart Cleveland, Ohio 44112 Fort Worth, Texas COLUMN TUBING ELECTRONICINTEGRA~ORS SUPERIOR TUBE COMP ANY Norristown, Pennsylvania DATEX CORPORATION 1307 South Myrtle Avenue Monrovia, California GASES AIR PRODUCTS AND CHEMICALS, INC. INFOTRONICS CORPORATION Allentown, PennsyIvania 7800 Westgien Drive Houston, Texas 77042 AIR REDUCTION SALES COMP ANY Div. of Air Reduction Co., Inc. SYRINGES 150 East 42nd Street New York 17, New York HAMILTON COMPANY P. O. Box 307 LINDE AIR PRODUCTS COMPANY Whittier, California A Division of Union Carbide Corp. East Park Drive and Woodward Avenue INFRARED ACCESSORIES Tonawanda, New York BARNES ENGINEERING COMPANY THE MATHESON COMPANY, INC. Instrument Division P. O. Box 85 Stamford, Connecticut East Rutherford, New Jersey

WILKS SCIENTIFIC CORPORATION PRECISION GAS PRODUCTS, INC. 140 Water Street Box 364 South Norwalk, Connecticut Westfield, New Jersey 07091 Bibliography

BASIC TEXT BOOKS GAS CHROMATOGRAPHY, D. Ambrose and Barbara A. Ambrose, 220 pp, $6.75 (0. Van Nostrand Co., Inc., 1962).

GAS CHROMATOGRAPHY. Ernst Bayer. 240 pp, $5.00 (English translation of 1st German ed.) (American Elsevier Publishing Co., Inc .. 1961).

GAS PHASE CHROMATOGRAPHY, 3 Vo1s. (in English), Rudo1ph Kaiser. translated by P. H. Scott. Vol. I, GAS CHROMATOGRAPHY, 199 pp, $7.95, Vol. 11, CAPILLARY CHROMATOG­ RAPHY. 120 pp. $6.95. Vol. 111, TABLES FOR GAS CHROMATOGRAPHY, 162 pp, $7.75 (Butterworths. Inc., 1963).

GAS CHROMATOGRAPHY, A. I. M. Keu1emans, 217 pp (2nd ed. 1959), 234 pp, $7.50 (Reinhold Publishing Corp., 1957).

GAS CHROMATOGRAPHY, J. H. Knox, 126 pp, $3.25 (John Wiley & Sons, Inc .. 1962).

GAS CHROMATOGRAPHY: PRINCIPLES, TECHNIQUES ANDAPPLICATIONS, A. B. Littlewood, 507 pp. $15.00 (Academic Press, Inc .. 1962).

GAS LIQUID CHROMATOGRAPHY: THEORY AND PRACTICE, Stephen Dal Nogare and Richard S. Juvet. 450 pp. $13.95 (John Wiley & Sons, Inc .. 1962).

GAS CHROMATOGRAPHY. Howard PurneU. 441 pp. $12.00 (John Wiley & Sons, Inc .. 1962).

SYMPOSIA PROCEEDINGS VAPOR PHASE CHROMATOGRAPHY - 1956, D. H. Desty, ed.. 436 pp, $12.00.

GAS CHROMATOGRAPHY - 1958, D. H. Desty. ed•• 383 pp, $13.00.

GAS CHROMATOGRAPHY - 1960, R. P. W. Scott, ed .. 466 pp, $17.50 (Butterworths, Inc .. 1960).

GAS CHROMATOGRAPHY - 1962, M. Van Swaay, ed., 411 pp, $19.95 (Butterworths, Inc .. 1963).

GAS CHROMATOGRAPHY: First International Symposium Held under the Auspices of the Analysis Instrumentation Division of the Instrument Society of America, Vicent J. Coates, Henry J. Noebe1s. and Irving S. Fagerson, eds .. 328 pp, $12.00 (Academic Press, Inc., 1958).

GAS CHROMATOGRAPHY: Second International Symposium Held under the Auspices of the Instrument Society of America, Henry Noebels, R. F. Wall, and Nathanie1 Brenner, eds., 463 pp, $16.00 (Academic Press, Inc., 1961).

GAS CHROMATOGRAPHY: Third International Symposium Held under the Auspices of the Analysis Instrumentation Division of the Instrument Society of America, Nathanie1 Brenner, Joseph E. Callan, and Marvin D. Weiss. eds .. 719 pp, $22.00 (Academic Press, Inc., 1962). 271 272 Bibliography

GAS CHROMA TOGRAPHY: Fourth International Symposium, Analysis Instrumenta tion Division, Instrument Society of America, June 1963, Lewis Fowler, ed., 270 pp, $10.50 (Academic Press, Inc., 1963).

APPLICATIONS BIOCHEMICAL APPLICATIONS OF GAS CHROMATOGRAPHY, H. P. Burchfield and E1eanor E. Storrs, 680 pp, $22.00 (Academic Press, Inc .. 1962).

STEROlD CHROMATOGRAPHY, Robert Neher, approx. 275 pp, $1l.00 (American Elsevier PubJishing Co., Inc., 1964).

BIOMEDICAL APPLICATIONS OF GAS CHROMATOGRAPHY. H. A. Szymanski, ed .. 330 pp, $12.50 (Plenum Press, 1964).

LECTURES ON GAS CHROMATOGRAPHY - 1964: AGRlCULTURAL AND BIOLOGlCAL APPLICATIONS, H. A. Szymanski and L. R. Mattick, eds .. 3OOpp, $12.50 (Plenum Press, 1965).

CHROMATOGRAPHY, Erich Heftmann, ed., 753 pp. $17.50 (Reinhold Publishing Corp., 1965).

OPEN TUBULAR COLUMNS IN GAS CHROMATOGRAPHY, L.S. Ettre, 184 pp, $4.95 (Plenum Press. 1965).

REVIEWS CHROMATOGRAPHY: A REVIEW OF PRlNCIPLES AND APPLICATIONS, E. Lederer and M. Lederer. 2nd ed. (Ist ed. 1955),712 pp. $14.00 (American Elsevier PubJishing Co., Inc .. 1957).

CHROMATOGRAPHIC REVIEWS, M. Lederer (American Elsevier PubJishing Co., Inc.). Val. H, 195 pp, $9.00 (1959), Vol. IV, 184 pp, $9.50 (1961), Vol. V, 244 pp, $11.00 (1962), Vol. VI, 228 pp, $12.50 (1963).

JOURNALS JOURNAL OF CHROMATOGRAPHY, Vols. 1-4. per vol. $18.00 (1958-1960), Vols. 5-6, per vol. $18.00 (1961), Vois. 7-9, per vol. $18.00 (1962), Vols. 10-12, per vol. $15.00 (1963), Vols. 13-16. per vol. $15.00 (1964). Single issues, per 100 pp, $2.80. Vols. 17-20, per vol. $17.50 (1965) (American Elsevier Publishing Co .. Inc.).

JOURNAL OF GAS CHROMATOGRAPHY. Published monthly. One year subscription: $10.00 in the U. S.; $12.00 all other countries (Preston Technical Abstract Co.).

ABSTRACTS GAS CHROMATOGRAPHY ABSTRACTS - 1958, C. E. H. Knapman, ed., 262 pp, $8.50 (1960).

GAS CHROMATOGRAPHY ABSTRACTS - 1959, C. E.H. Knapman, ed.. 164 pp, $8.50 (1960).

GAS CHROMATOGRAPHY ABSTRACTS - 1960, C. E. H. Knapman, ed., 164 pp. $8.50 (1960).

GAS CHROMATOGRAPHY ABSTRACTS - 1961, C. E. H. Knapman, ed.. 219 pp, $8.50 (1962).

GAS CHROMATOGRAPHY ABSTRACTS - 1962, C. E. H. Knapman, ed .. 203 pp, $8.50 (1963) (Butterworths, Inc.).

GAS CHROMATOGRAPHY ABSTRACT SERVICE. Published weekly on punched cards (Pres­ ton Technical Abstract Co.).

COMPREHENSIVE BIBLIOGRAPHY TO THE LITERATURE ON GAS CHROMATOGRAPHY, Seaton T. Preston, ]r .. and Geneva Hyder, $12.00 (Preston Technical Abstract Co., 1964). Bibliography 273

GUIDE TO GAS CHROMATOGRAPHY LITERATURE, A. V. Signeur, 360 pp, $12.50 (Plenum Press, 1964).

GUIDE TO GAS CHROMATOGRAPHY LITERATURE. SUPPLEMENT NO. 1, A. V. Signeur, approx. 350 pp, about $12.50 (Plenum Press, 1965). Author Index

A C Aaker, A. A., 46 roaJ Calcote, H. F., 32 [15,lS] Abbott, J. P .• 63 r8J Camacho, A. M., 183 [ls] Adlerkreutz, H., 63 ~2J Campbell, S., 65 f9) Ahmad, N., 64 ~9J, 183 [27J Capizzi, R. L., 64 ~3) Allen, W. M., 129 [1] Carr, H. E .. 65 f4,S5), 209 [5] Arnold, J. E., 83 f] Cawley, L. P .. 65 f9) Averill, W., 30 [11] Chattoraj, S. C., 64 ~,45,51), 191 r1), 245 [18] B Chen, C., 61 [8] Bailey, E., 64 [58,59],212 [14], 213 [16], Cheshire, J. D., 16 ~) 218 [17] Claggett, W. R., 63 [l8] Barnes, A. C., 63 r1], 150 [12] Clark, S. J., 64 [54), 96 r), 251 [19) Barton, D. H. R., 108 [12,13] Claussen, B., 169 [17], 174 [17) Bate-Smith, E. C., 100 r] Clayton, G. W.. 201 [2) Bauld, W. S., 225 r], 226 f], 261 [20] Clayton, R. B., 102 [5,S,7), 104 f,7). Bedford, A. R., 226 [13] 109 (), 113 f) Bender, S. R., 64 ~3] Cleveland, W. W., 64 ~9], 183 [l7) Bergmann, W., 85 [10] Cohen, R., 63 r1] Bernstein, M., 51 r] Cohn, E. S., 100 [2] Berthuis, R. K., 61 [5] Collins, W. P., 63 [36), 150 [lS] Beynon, J. H., 54 [19] Condon, R. D., 30 [11) Bhacca, N. S., 53 [12] Cook, L. R., 130 [2) Biemann, K., 54 [15, 18], 55 [15] Cooper, J. A., 63 [20,28) Bongiovanni, A. M., 201 r], 202 ~] Corner, G. W., 129 [1] Bonnano, P., 63 r2] Cox, R. 1., 63 [34], 202 [5),226 [13) Bradlow, H. L., 212 [11] Cox, R. T., 132 [7) Brane, E. G., 53 [13J Creech, B. G., 63 [20,23). 64 [56], 167 Breuer, H .. 225 [1] [13, 13a), 173 (13) Brooks, C. J. W., 65 [70],83 [8],108 [14], 109 [14], 113 [14], 202 f] D Brooks. R. V., 64 ~8], 180 r5] Dal Nogare, S., 52 f) Brown-Grant, K., 65 [74] DeLaat, B. M., 166 [2) Brown, J. B., 62 [18,19], 131 r], 225 r], DePaoli, J. C., 169 (18), 199 (18) 226 [10], 246 r], 255 [2), 263 [2) Desty, ~. H., 16 [5),30 (9) Brownie, A. C., 64 [52), 192 r2) Dewar, R. A., 30 [8,9), 31 [8), 62 [15) Budzikiewicz, H., 54 [16) Dingernanse, E., 166 [l) Bulbrook, R. D., 172 [19) Djerassi, C., 52 ~), 54 (16) Bush, 1. E .• 102 ~]. 104 ~], 174 [21) Dobriner, K., 52 [5], 203 [10] 275 276 Author Index

Dorfrnan. L.. 51 [2] H Dorfman. R. 1.. 63 [26]. 64 ~6]. 150 [13]. Haahti. E. O. A .• 62 [9.13). 122 [19). 166 [7].167 [14.15].178 [22). 17ge3). 167 [11). 226 [11).227 [11) 191 e2• 29 ). 201 e). 226 [5) Hamilton. R. J .. 61 ~). 122 e9a ] Drosdowsky. M .• 64 ~6]. 179 [23) Hanaineh. L .. 108 [14]. 109 [14). 113 [14] Dusza. J. P .. 51 [3) Harley. J .• 30 [7]. 31 [7). 62 [14] Harris. R. J .• 25 e] Hartman. 1. S .• 63 el ). 96 [2).107 [9.10). E 108 [10). 109 [10). 113 [9.10]. 122 [10). Eberlein. W. R .• 202 ~] 123 [10]. 167 [12). 169 [12) Eglinton. G .. 61 ~) Haslewood. G. A. D .• 65 [71] Eik-Nes. K. S .• 64 [52). 150 [11.14). 169 Hayano. M .• 201 [1) [18). 192 [32). 199 [18) Hechter. 0 .• 201 [1) Eneroth. P .• 65 r6 ) Heller. M •• 51 [3) Engel. L. L .• 51 [3) Hellman. L.. 212 [11) England. A .. 100 [2) Hellstrum. K .. 65 r6) Hodges. R .• 61 ~] Horning. E. C .• 61 r]. 62 [9.13].63 [23.41). F 74 [3). 79 83 [7). 96 [1]. 122 l r). Fales. H. M .• 64 r ). 83 r). 209 [8). [18.19.19a). 167 [11.13). 173 [13].209 212 [8) ~). 226 [7).226 [11].227 [11.16) 9 Faucette. W .. 65 r ) Huis int Veld. L. G .• 166 [2] Finkelstein. M .• 191 [29) Hurlock. S .• 212 [12) Fishman. J .• 62 [18). 226 [10) Forchielli. E .• 65 [74). 191 e9) France. J. T .• 63 [26).167 [14.15) I Frawley. T .• 63 [32) Ibayashi. H .• 64 ~7]. 179 [24). 183 [24) Friedal. R. A .• 62 [12) Ittrich. G .• 225 ~l Fukushima. D. K .• 207 [1]. 212 [11] Futterweit. W.• 64 ~6]. 150 [13]. 179 [23] J James. A. T .• 8 [2].30 [5]. 61 [1.2) G Jandorek. R. D .• 64 [55]. 202 [7) Gabrilove. J. L.. 64 [51). 191 fl) Jansen. A. P .• 63 e3 ] Gallagher. S. S.• 52 [5) Jardetzky. C. D •• 53 [9] Gallagher. T. F .• 203 [9).212 [11) Jardetzky. 0 .• 53 [9] Gallaway. W. S.• 32 [17). 33 [17) Jenkin. D .• 209 e) Gardner. L. T .• 166 [8) Jones. D. T. L.. 32 [17]. 33 [17] Gershlein. L. L .• 65 r8) Jones. R. N .• 52 [5) Giuliani. G .• 180 [25) Juvet. R. S.• 52 r] Givner. M. L .• 261 [20) Glick. D •• 53 [9). 226 r) Goldfien. A .• 63 f5). 150 [15) K Goodno. J. A .• 150 [12) Kappas. A .• 203 [9) Gottfried. H •• 77 [1).210 [9) Katzenellenbogen. E. R .• 52 (5) Gould. G. R .• 64 rO) Keefer. C. S .• 131 [5) Gould. R. G .• 212 [13) Kellie. A. E .. 166 [5) Goulden. F .• 166 r) Keutmann. E. H .• 63 [22]. 167 [10] Gower. D. S .• 65 [71) King. 1. R •• 32 [15] Greenway. 226 r) Kirschner. M. A .• 63 f4.25]. 64 [24.61). Groen. D .• 63 [37). 154 [18) 65 [73]. 131 ~]. 138 ~]. 168 [16). Gudzinowicz. S. J .• 33 [18) 172 [16]. 202 [8]. 209 [8]. 212 [8] Guerra-Garcia. R .• 64 [51]. 191 rl ] Kittinger. G. W•• 64 [57].209 [7] Author Index 277

Kitty. W.• 261 ro) Michie.E. A .• 131 [3) Kliman. S .• 81 ~) Migeon. C. J .. 166 [9). 183 [26) Klinkenberg. A .• 13 [3) Mikkelsen. 1.. 82 [5) Klopper. A .• 130 r). 131 f). 145 r) Moscatelli. E. A .• 74 f) Knights. S .• 84 [9J Mozden. P. J .• 63 [30). 131 [5) Knights. S. A .• 65 [12). 104 [8). 107 [8). Murakawa. S .• 64 ~7). 179 [24). 183 [24) 108 [8). 113 [8.16) Murawec. T .• 63 [40). 226 [14] Kroman. H. S .• 64 ~3) Murphy. 0 .• 218 [11) Kumar. 0 .• 150 [12) Musser. S.O .• 65 r9)

N L Nakamura. M .• 64 ~7). 179 [24).183 [24) Lakshmanan. T. K .• 166 ~] Nakao. K .• 64 ~7). 179 [24). 183 [24) Landowne. R. A .• 62 [10). 64 [53). 109 Naukkarinen. 1.. 65 ~). 209 [5) [15). 154 9) e Nel. W.• 30 [7). 31 [7] Langer. S. H .• 62 [12) Nes. W. R .• 174 [20) 3 Lantos. C .• 64 ~6). 179 e) Nikolski. A .• 63 ~o) Lantz. C. 0 .• 61 [8) Nishizawa. E. E .• 64 [52). 150 [14]. 169 Lebeau. M. C .• 64 [55). 202 [7) [18). 192 f2). 199 [18] Lemon. H. M .• 166 [1) Norymberski. J .K .• 26[3).65[63).152[17). Lieberman. S .• 166 ~) 202[6). 209f). 213[15). 219[15) Lipsett. M. S .• 63 [24.25). 64 r4). 65 [73). 131 ~). 138 ~). 168 [16). 172 [16). 202 [8) o Lipsky. S. R .• 30 [12). 62 [10). 64 [53). Oertel. G. W•• 150 [11) 109 [15). 154 [19) O'Neill. H. J .. 65 r8) Loraine. J. A .• 145 [8) Onkiehong. 1.. 30 [10) Lovelock. J. E .• 30 ~.5.6.12.13.14).42 [14). 43 [14.19.20) Luetscher. J. A .• 64 ro). 212 [13) p Luukkainen. T .• 62 [13). 63 ~1.42]. 226 [11).227 [11) Patti. A. A .• 12 ea ). 63 [32) Pincus. G .• 166 [7) Piper. E. A .• 30 [5) M Plager. J. E .• 166 [9) Preedy. J. R. K .• 226 [5) McComas. D. S .• 63 [35). 150 [15) Pretorius. V .• 30 31 [7). 62 [14) McLafferty. F. E .• 54 [17) e). Purnell. J. H .• 17 18 [7) McNiven. N. 1.. 63 [26). 64 ~6).150 [13). r). 167 [14.15). 179 [23) McWilliam. 1. G .• 30 [8.9].31 [8). 62 [15) R Makita. M .• 65 r7] Marcus. 1.. 64 ~6). 179 [23) Raphael. R. A •• 61 ~] Marcus. P .• 166 [1) Ray. N. H .• 61 f) Martin. A. J. p .. 8 [1.2].61 [1.2]. 100 Recourt. J. H .• 61 [5] (1) Reddy. W. J .. 209 [2) Martin. H. F .• 61 [7]. 62 [11.16.17). 63 Rivera. R .• 167 [15] (17). 77 [2). 113 [17). 123 [17). 226 Rob~rts. G •• 52 [5) [8.9]. 227 [8). 232 [17) Robinson. A. M •• 166 f] Mason. W. S .• 63 r2]. 167 [10] Rogers. A. W.• 65 [12] 2 Menini. E .• 26 f). 65 r3). 152 [17). 213 Rosenfeld. R. S •• 64 [55). 65 r ). 202 [7). [15], 219 [15] 211 [10] Merits. 1.. 209 r) Rosenfelder. W. J .• 108 [13) 278 Author Index

Rosengreen. S. K .• 63 [28] U Rubin. S. L.. 166 [7] Uchikawa. T .• 64 ~7J. 179 [24]. 183 [24] Ryan. K. ] .• 191 [30] Umbreit. G. R .• 23 [I]. 50 [1] Ryhage. R .• 54 [14]. 65 rS]

V S van Deemter.]. ] .• 13 e] Saba. N .• 201 [1] VandenHeuvel. W.]. A •• 43 fO]. 61 r]. Sabima. T., 64 [55] 62 [9.13). 63 [23.41J. 79 [3]. 83 [7J. 96 [1). 122 [18.19.198). 167 [11.13]. Salumaa. T., 202 [7] 173 [13].209~].226[7.11).227 [11.1S] Sandberg. D. H., 64 ~9], 183 [27] Sarios, 1. ] .• 63 fl] VanderMolen. H .• 63 e7]. 64 [52]. 154 Savard. K., 64 ~9], 108 [11],166 [l,S], [18 J. 192 f2] 183 f7] vanGent. C. M .• 122 [20]. 128 f O] Scholly, P. R .• 30 [11] Veron, H. H .. 226 [14J Scott, R. P. W .• 16 ~.5]. 30 r,l1] Vestergaard. P .• 169 [17]. 174 [17) Seegar-]ones. G.E .• 63 [31] Segal, S .• 174 [20] Segal. L .• 174 [20) W Sharkey. A. C .• 62 [12] Wade, A. P .• 166 [5J 5 Short. R. V., 150 [10] Watson. ]. T .• 54 r J. 55 [15J Simmer. H .• 150 [9] Weiss. S. P •• 150 [11) Simmonds. P. G., 43 [20] Weissberger, A .. 53 [10] 7 Sjovall. ] .• 96 [1].227 [lS) Wells. W .• 65 r ] Sommerville, 1. F .• 63 es]. 150 [lS] Wender. ] .• 62 [12] Sparagana. M., 63 f2]. 64 [50], 167 [lO J. West. H. F .. 218 [17] 183 f8J Westall. R. G .• 100 e] Spencer. S .• 82 [5] Whittier. M. S •• 23 [1). 50 [1]. 82 [5]. 87 [1] Stavely. H. E .• 85 [10] 2 Stein, A. A .• 12 fa]. 63 [32] Williams. D. H .• 53 rt ). 54 [lSJ Wilson. H .• 65 [73] Stern, M. 1.. 202 f] Woodford. F. P .. 122 [20],128 [20] Sternberg, ]. C .. 32 [17J, 33 [17J Wotiz. H. H .• 61 [7]. 62 [11. lS. 17]. 63 Stothers. ]. S., 53 [10. 11 J [17.21.29.30.38). 64 ~,45.51.54]. 65 Strong.]. A .• 130 [2J ~. S5). 77 [2), 96 [2.3), 107 [9.10]. Sweeley, C. C .• 61 r], 74 f]. 79 [3J. 226 108 [10). 109 [10), 113 [9.10.17]. 122 (7) [10]. 123 [10. 17J. 131 [5]. 132 rJ, 166 Synge. R. L. M., 8 [1] [1]. 167 [12]. 169 [12]. 191 f 1J,20!J [5]. 226 [8.9). 227 [8). 232 [17). 245 [18). 251 [19J

T Talalay. P., 212 [12] y Tanioka. T., 64 ~7J. 179 [24]. 183 [24] Yannone. M. E •• 63 f5]. 150 [15) Tenney, H. M., 25 f] Young. ]. S.• 65 [70] Thomas. S. S •• 63 [27], 172 [19] Yousem, H. L •• 63 f9] Thomas. G. H .. 65 [72.74], 104 [8]. 107 Yousem. ]. L.. 226 [12] [8]. 108 [8], 113 [8,lS] Thorn. G. W., 209 (2) Z Touchstone. ]. C., 63 ~oJ. 226 [14,15]. Zander. ] .• 150 [9] 232 [15] Zuiderweg, F. ]., 13 e] Turner, D. A .• 63 [31J Zumoff. S .• 212 [11) Subject Index

A 5ß-Androstane-3,17 -dione, 83, 106, ACTH, effect on pregnanediol excre­ 110, 117, 118, 119, 120 tion, 145 5a-Androstan-3ß-ol, 106, 108, 109, Adrenocortical steroids, determina­ 118, 119 tion of, 207 5a-Androstan-3ß-ol acetate, 108 Adsorptivity, loss of sampIe by irre­ 5ß-Androstan-3a-ol, 110 versible adsorption, 87 5ß-Androstan-3ß-ol, 106, 118, 119 Aldosterone, (see 11ß,21-dihydroxy- 5a-Androstan-17a-ol, 109 3,20-dioxopregn-4-en-18-al) 5a-Androstan-17 ß-ol, 83, 106, 109, Aldosterone diacetate, (see 11ß,18- 112, 118, 119 epoxy-18,21-dihydroxypregn-4- 5ß-Androstan-17 ß-ol, 106, 118, 119 ene-3,20-dione 18,21 diacetate) 5a-Androstan-17ß-ol methyl ether, Allopregnanediol, (see 5a-pregnane- 116, 120 3a,20a-diol) 5a-Androstan-3-one, 106, 108, 109, Allopregnanediol diacetate, (see 5a­ 118, 119 pregnane-3a,20a-diol diacetate) 5ß-Androstan-3-one, 106, 118, 119 Allotetrahydrocortisol, (see 3a,11ß, 5a-Androstan-17-one, 106, 109, 112, 17 a,21-tetrahydroxy-5a- pregnan- 118, 119 20-one) 5ß-Androstan-17-one, 106, 118, 119 Allylic alcohols, thermal decomposi- 5ß-Androstane-3,11,17-trione, 105, tion of, 84 110, 117,206,211 Androgens, 165 Androst-4-ene-3a,17 ß-diol, 83 Androstanes, 61 Androst-5-ene-3ß,17 ß-diol, 110, 124, Androsta-2,4-dien-17-one, 85 127, 132, 169, 181 Androsta-3,5-dien-17-one, 85 Androst-5-ene-3ß ,17 ß-diol diacetate, 5a-Androstane, 106, 109, 112, 116, 181 117,118 Androstenedione, (see androst-4-ene- 5ß-Androstane, 106, 117, 118, 119 3,17-dione) 5a-Androstane-3a,17 ß-diol, 83, 106, Androst-4-ene-3,16-dione, 124 118, 119 Androst-4-ene-3,17-dione, 65, 83, 110, 5a-Androstane-3ß,17ß-diol, 83, 106, 124, 127, 156, 165, 180, 181, 192, 109, 118, 119, 120 193, 194, 241, 242 5ß-Androstane-3a,17 ß-diol, 106, 110, Androst-5-en-3ß-ol, 110 118, 119 Androst-5-en-17 ß-ol, 110, 112 5ß-Androstane-3ß ,17 ß-diol, 106, 118, Androst-16-en-3a-ol, 65 119,120 Androst-4-en-3-one, 110 5a-Androstane-3ß,17 ß-diol dimethyl 5a-Androst-2-en-17-one, 110 ether, 116, 120 Androst-4-ene-3,6,17-trione, 110 5a-Androstane-3,17-dione, 106, 108, Androst-4-ene-3,11,17-trione, 110, 124, 109, 118, 119, 120 165, 194 279 280 Subject Index

Androsterone, (see 3a-hydroxy-5a­ Cholest-2-en-5a-ol, 120 androstan-17-one) Cholest-7-en-3ß-ol, 124 Androsterone acetate, (see 3a-hy­ Cholest-4-en-3-one, 111, 124, 127 droxy-5a-androstan-17 -one ace­ Cholest-5-en-3-one, 111 tate) Cholesterol, (see cholest-5-en-3ß-ol) Androsterone TMSi, (see 3a-hydroxy- Cholesterol mono chloroacetate, (see 5a-androstan-17 -one tri methyl­ cholest-5-en-3ß-ol monochloroace­ sily1 ether) tate) Argon ionization detector, 30, 62 Cholesterol trifluoroacetate, (see cho­ lest-5-en -3 ß-01 trifluoroacetate) B Cholestenone, (see cholest-4-en-3-one) Binomial distribution, 9 Chromatogram baseline,6 C differential, 5 peak,6 Carrier gas, definition, 3 peakarea,6 5a-Cholane, 111 peak height, 6 5ß-Cholane, 111, 117 peak width, 6 5a-Cholan-12a-ol, 111 peak width at half height, 6 5ß-Cholan-12a-ol, 111, 117 Chromatography, definition, 3 5a-Cholan-12-one, 111 Cistestosterone, (see 17a-hydroxyan­ 5ß-Cholan-12-one, 111, 117 drost-4-en-3-one) Cholesta-5,24-dien-3ß-ol, 124 Column 5a-Cholestane, 111, 112 conditioning,75 5ß-Cholestane, 111, 112, 116, 117, configuration, 69 181, 190, 214 definition, 3 5ß-Cholestane-3ß, 7ß-diol dimethyl diameter, 70 ether, 116, 120 effect of tubing material on sampie 5ß-Cholestane-3ß,7ß-diol dimethyl stability, 82 ether, 116, 120 filling,74 5a-Cholestane-3,6-dione, 111 preparation, 73 5a-Cholestan-3a-ol, 111 theory,5 5a-Cholestan-3ß-ol, 108, 111 tubing material, 69 5a-Cholestan-3ß-ol acetate, 108 Column performance, 13 5ß-Cholestan-3a-ol, 111 band spreading from path length 5ß-Cholestan-3ß-ol, 111 differences, 14 Cholestan-7a-ol, 116, 120 diffusion in the gas phase, 15 5ß-Cholestan-7a-ol methyl ether, 116, effect of carrier gas molecular 120 weight on, 15 5ß-Cholestan-7 ß-ol methyl ether, 116, effect of carrier gas velocity on, 120 15 5a-Cholestan-3-one, 108, 111 effect of gas pressure on, 15 5a-Cholestan-6-one, 111, 112 effect of particle size on, 14 5ß-Cholestan-3-one, 111 radial diffusion in the gas phase, 5a-Cholest-2-ene, 111, 116, 120 17 Cholest-5-ene, 111, 112 resistance to mass transfer, 16 Cholest-8-ene, 116 stationary phase distribution, 16 Cholest-4-ene-3,6-dione, 111 vanDeemter equation, 13 Cholest-5-en-3ß-ol, 111, 124, 127, 136, Column ovens, 29 127, 194,241,242 Column packing, Cholest-5-en-3ß-ol monochloroace­ influence on sam pie decomposition, tate, 195 80 Cholest-5-en-3ß-ol trifluoroacetate, Cortexolone, (see 17 a,21-dihydroxy­ 127 pregn-4-ene-3,20-dione) Subject Index 281

Corticosteroids, 64 Detectors, 30 analysis of bismuthate oxidation DHEA, (see 3ß-hydroxyandrost-5- products, 221 en-17-one) periodic oxidation of, 209 DHIA, (see 3ß-hydroxyandrost-5-en- pyrolysis in gas chromatograph, 17-one) 212 Dihydrotestosterone, (see 17 ß-hy• sodium bismuthate oxidation, 212 droxy-5ß-androstan-3-one) Corticosterone, (see 11ß ,21-dihy­ 3a,llß-Dihydroxy-5ß-androstan-17- droxypregn-4-ene-3,20-dione) one, 105,132, 169, 171,190,214 Cortisone, (see 17 a,21-dihydroxy­ 3a, llß -Dihydroxy-5a-androstan -17- pregn-4-ene-3,11,20-trione) one di-[trimethylsilyl ether], 214 Cortison acetate, (see 17a,21-dihy­ 3a,11ß-Dihydroxy-5ß-androstan-17- droxypregn-4-ene-3, 11, 20-trione one di [trimethylsilyl ether], 214 21-acetate) 3a,l1ß-Dihydroxy-5a-androst-17-one, Cortisol, (see 11ß,17a,21-trihydroxy• 132,169,171,173,190 pregn-4-ene-3,20-dione) 11ß,21-Dihydroxy-3,20-dioxopregn-4- Cortol, (see 5ß-pregnane-3a,l1ß ,17 a, en-18-al,65,194,209 20a,21-pentol) 3 ,16a-Dihydroxyestra-1,3,5 ( 10) trien- ß-Cortol, (see 5ß-pregnane-3a,l1ß, 17-one, 226, 229, 230, 241, 242, 17a,20ß,21-pentol) 254, 257 analysis as TMSi ethers, 219 3,16ß-Dihydroxyestra-1,3,5 (10) trien- glc as TMSi ethers, 211 17-one,226 Cortolone, (see 3a,17 a,20a,21-tetra­ 3,18-Dihydroxyestra-1,3,5 (10) -trien- hydroxy-5ß-pregnan-11-one) 17-one,226 ß-Cortolone, (see 3a,17 a,20ß ,21-tetra­ 3,17 ß-Dihydroxyestra-1,3,5 (10)­ hydroxy-5 ß -pregnan -ll-one ) trien-16-one, 226, 229, 230, 241, analysis as TMSi ethers, 219 254,257 glc as TMSi ethers, 211 2,3-Dihydroxyestra-1,3,5 (10) -trien- 17-one 2-methyl ether, 226, 229, D 230, 235, 241, 242, 243, 254, 257, Dead volume, or, gas hold-up volume, 261 6 17 a,21-Dihydroxypregna-1,4-diene- ll-Dehydrocorticosterone, (see 21- 3,11,20-trione,213 hydroxyp regn-4-en e-3, 11,2 0- 3O',12O'-Dihydroxy-5ß-pregnan-20- trione) one,105 Dehydroepiandrosterone, (see 3ß-hy• 30',17 a-Dihydroxy-5ß-pregnan-20- droxyandrost-5-en-17-one) one, 132, 169,202 Deydroepiandrosterone acetate, (see 3O',17O'-Dihydroxy-5ß-pregnan-20-one 3ß-hydroxyandrost-5-en-17-one 3-acetate, 133 acetate) 11ß ,21-Dihydroxypregn-4-ene-3,20- Dehydroepiandrosterone TMSi, (see dione,180,192,194,209 3ß-hydroxyandrost-5-en-17-one 18,21-Dihydroxypregn-4-ene-3 ,20- trimethylsilyl ether) dione,209 Dehydroisoandrosterone, (see 3ß-hy• 17O',21-Dihydroxypregn-4-ene-3,20- droxyandrost-5-en-17-one) dione,194,208,209 Desmosterol, (see cholesta-5,24-dien- 3ß,17O'-Dihydroxy-pregn-5-en-20- 3ß-ol) one, 105, 132, 169, 181 Desoxycortisone, (see 21-hydroxy­ 17O',21-Dihydroxypregn-4-ene-3,1l, pregn-4-ene-3,20-dione) 20-trione, 194,208, 209, 210, Derivatives of steroids, 213,216 acetates, 95 17O',21-Dihydroxypregn-4-ene-3,11, heptafiuorobutyrates, 96 20-trione 21-acetate, 81 trifiuoroacetates, 96 3ß, 70'- Dimethoxyallopregnane, (see trimethylsilyl ethers, 95 5O'-pregnane-3ß,7O'-diol dimethyl ether) 282 Subject Index

3ß,7ß-Dimethoxyallopregnane, (see Estra-1,3,5 (10) -triene-3,17ß-diol, 62, 5a-pregnane-3ß, 7ß-diol dimethyl 124, 127, 156, 194, 226, 229, 230, ether) 241, 242, 243, 244, 248, 256, 257, 260 E determination in low titer urine, Electrometer amplifier, 45 263 Electron capture detector, 36 determination in pregnancy urine, applied potential, 38 235,261 carrier gas flow rate, 38 fluorescence spectrum, 253 contamination of tritium source, Estra-1,3,5 (10) -triene-3,17 ß-diol 44 diacetate, 81, 233, 238, 239, 249, current voltage relationship, 37 251, 252, 253, 254 effect of column bleed on sensitiv- infrared spectrum, 78 ity,44 fluorescence spectrum, 239 electron capturing processes, 36 Estra-1,3,5 (10) -triene-3,17 ß-diol faults, 43 di-[heptafluorobutyrate],258 linearity, 42 nature of carrier gas, 42 Estra-1,3,5 (10) -triene-3,17 ß-diol pulsed operation, 38, 42 di-[trimethylsilyl ether], 254 response to different molecular spe­ Estra-1,3,5 (10) -triene-2,3,16a,17 ß• eies, 43 tetrol 2-methyl ether, 226 sensitivity, 41 Estra-1,3,5 (10) -triene-3,16a,17 ß• temperature, 40 triol, 62, 194, 226, 229, 230, 241, Epiandrosterone, (see 3ß-hydroxy- 242, 243, 244, 248, 254, 257, 260 5a-androstan-17-one) determination in low titer urine, 16-Epiestriol, (see estra-1,3,5 (10)­ 263 triene-3,16ß,17 ß-triol) determination in pregnancy urine, 16-Epiestriol tri acetate, (see estra- 235,261 1,3,5 (10) -trien-3,16ß,17 ß-triol rapid determination in pregnancy tri acetate ) urine, 232, 258 Epistestosterone, (see 17 a-hydroxy­ Estra-1,3,5 (10)-triene-3,16a,17ß• androst-4-en-3-one) triol triacetate, 81, 233, 238, 239, Epitestosterone acetate, (see 17 a-hy­ 240, 250, 252, 253, 254 droxyandrost-4-en-3-one acetate) infrared spectrum, 79, 237 11ß,18-Epoxy-18,21-dihydroxypregn- acetylation, 229 4-ene-3,20-dione 18,21-diacetate, Estra-1,3,5(10)-triene-3,16a,17ß- 81 triol tri [heptafluorobutyrate], Equilenin, (see 3-hydroxyestra-1,3,5 258 (10) ,6,8-pentaen-17-one) 5a-Ergosta-7,22-diene, 112, 116 Estra-1,3,5 (10) -triene-3,16a,17ß• triol tri-[trimethylsilyl ether], Ergostane, 116 5a-Ergost-7-ene, 112, 116, 120 254 5a-Ergost-8 (14) ene, 112, 116 Estra-1,3,5 (10) -triene-3,16ß,17 ß• Ergost-7-en-5a-ol,120 triol, 226, 229, 230, 241, 242, 243, Estradiol-17ß, (see estra-1,3,5(10)­ 254, 257 triene-3,17ß-diol) determination in pregnancy urine, Estradiol diacetate, (see estra-1,3,5 261,235 (10) -triene-3,17 ß-diol diacetate) Estra-1,3,5, (10) -triene-3,16ß ,17 ß• Estradiol TMSi, (see estra-1,3,5 (10)­ triol triacetate, 233 triene-3,17 ß-diol di- [trimethyl­ Estriol, (see estra-1,3,5 (10) -triene- sily1 ether] ) 3,16a,17 ß-triol) Estradiol heptafluorobutyrate, (see Estriol triacetate, (see estra-1,3,5 estra-1,3,5 (10) -trien-3,17ß-diol (10) -triene-3,16a,17 ß-triol tri ac­ di-[heptafluorobutyrate] ) etate) Subject Index 283

Estriol heptafiuorobutyrate, (see linearity, 32 estra-1,3,5 (10) -triene-3,16a, mechanism of operation, 31 17 ß-triol tri [heptafiuororobuty­ response to different molecular spe- rate]) eies, 34 Estriol trimethylsilyl ether, (see sensitivity, 32 estra-1,3,5 (10) -triene-3,16a, Fog formation, 49 17 ß-triol tri-[trimethylsilyl ether] ) Estrogens, 61 G acetylation of, 229 Gas chromatography, definition, 3 characterization by glc, 254 Gas liquid chromatography, (GLC) choice of derivative for glc, 227 definition, 3 comparison of methods for deter- Gas liquid partition chromatography, mination in urine, 256 (GLPC) definition, 3 determination in urine, 225 Gaussian distribution, 9 determination of ring D-a-ketols Glucocorticoids, 64 in pregnancy, 235 ß-Glucuronidase, 146 excretion during menstrual cycle, 247,248 excretion du ring normal preg- H nancy, 243 Height equivalent to a theoretical excretion in adult women, 246 plate (HETP), 10 experimental methods, 252 Henry's law, 11 measurement of, 260 7ß-Hydroxyandrosta-1,4-dien-3-one, properties of derivatives, 228 190 Estrogen acetates, 227 3a-Hydroxy-5a-androstane-11,17- trifiuoroacetates, 227 dione,190 trimethylsilyl ethers, 227 3a-Hydroxy-5ß-androstane-11,17- Estrone, (see 3-hydroxyestra-1,3,5 dione, 110, 132,169, 171,173, (10) -trien-17-one) 190,192,193,214,241,242 Estrone acetate, (see 3-hydroxyestra- 3a-Hydroxy-5 ß -androstane-11,l 7- 1,3,5 (10) -trien-17-one acetate) dione trimethylsilyl ether, 214 Estrone heptafiuorobutyrates (see 3- 3a-Hydroxy-5a-androstan-17-one, hydroxyestra-1,3,5 (10) -trien-17- 106,109,117,118,119,132,156, one heptafiuorobutyrate) 166,169,171,172,173,180,181, Estrone TMSi, (see 3-hydroxyestra-1, 182,190,192,193,194,241 3,5 (10) -trien-17-one tri methyl­ 3a-Hydroxy-5a-androstan-17-one ace­ silyl ether) tate, 133, 180, 181 Etiocholanolone, (see 3a-hydroxy-5ß• 3a-Hydroxy-5a-androstan-17-one tri­ androstan-17-one) methylsilyl ether, 214 Etiocholanolone acetate, (see 3a-hy­ 3a-Hydroxy-5ß-androstan-17-one, droxy-5ß-androstan-17-one 106,110,117,118,119,132,156, acetate) 166,169,171,172,173,180,181, Etiocholanolone TMSi, (see 3a­ 190,192,193,194,241,242 hyroxy-5ß-androstan-17-one 3a-Hydroxy-5ß-androstan-17-one trimethylsilyl ether) acetate, 133, 180, 181 3a-Hydroxy-5ß-androstan-17-one• F trimethylsilyl ether, 214 Flame ionization detector 31 3ß-Hydroxy-5a-androstan-17-one, applied potential, 32 106,109,118,119,166,169 carrier gas, effect on sensitivity, 33 3ß-Hydroxy-5ß-androstan-17-one, faults, 35 106, 118, 119 gas fiow rates, effect on sensitivity, 17a-Hydroxy-5a-androstan-3-one, 33 106, 118, 119 284 Subjcct Index

17a-Hydroxy-5ß-androstan-3-one, 20ß-Hydroxycortisone, (see 17a,20ß, 106, 118, 119 21-trihydroxypregn-4-ene-3,11- 17 ß-Hydroxy-5ß-androstan-3-one, dione) 106,110,117,118,119,120 6ß-Hydroxycortisone, (see 6ß,17a, 17 ß-Hydroxy-5a-androstan-3-one, 21-trihydroxypregn-4-ene-3,11, 106,109,117,118,119,120 20-trione) 11a-Hydroxyandrost-4-ene-3,17- 18-Hydroxydesoxycorticosterone, dione,124 (see 18,21-dihydroxypregn-4- 11ß-Hydroxyandrost-4-ene-3,17- ene-3,20-dione) dione,110, 124, 127,209 3-Hydroxyestra-1,3,5, (10) ,6,8- 3a-Hydroxyandrost-4-en-17-one, pentaen-17-one,126 84 3-Hydroxyestra-1,3,5 (10) -triene-16, 17-dione, 241, 242 3ß-Hydroxyandrost-4-en-17-one, 3-Hydroxyestra-1,3,5 (10) -trien-17- 84 one,62, 124, 127, 156,194,226, 3ß-Hydroxyandrost-5-en-17-one, 229,230,241,242,243,244,248, 65,106,110,118,119,124,127, 254,256,257,260 132,156,165,169,171,173,180, determination in low titer urine, 190,192,193,194,241,242 263 3ß-Hydroxyandrost-5-en-17-one determination in pregnancy urine, acetate, 133, 180, 181 235 3ß -Hydroxyand rost-5-en -17 -one rapid determination in pregnancy trimethylsilyl ether, 214 urine, 232, 258, 261 17 a-Hydroxyandrost-4-en-3-one, 3-Hydroxyestra-1,3,5 (10)-trien-17- 110,181,182,190,194 one acetate, 78, 81, 233, 238, 239, 17 a-Hydroxyandrost-4-en-3-one 240,250,252,253,254 acetate, 181, 189 loss on column, 88 17 ß-HYdroxyandrost-4-en-3-one, infrared spectrum, 236 64,104,110,124,127,156,165, 3-Hydroxyestra-1,3,5 (10) -trien-17 - 180,181,182,184,185,190,192, one hcptafluorobutyrate, 258 193,194,241,242 3-Hydroxyestra-1,3,5 (10)-trien-17- determination in urine, 178 one trimethylsilyl ether, 254 determination in plasma, 191 16a-Hydroxyestrone, (see 3,16a­ 17 ß-Hydroxyandrost-4-en-3-one dihydroxyestra-1 ,3,5 (10) -trien- acetate, 180, 181,189 17-one) 17 ß-Hydroxyandrost-4-en-3-one 16ß-Hydroxyestrone, (see 3,16ß• chloroacetate,157,195 dihydroxyestra-1,3,5 (10) -trien- 11ß-Hydroxyandrosterone, (see 3a, 17-one) 11ß-dihydroxy-5a-androst-17- 18-Hydroxyestrone, (see 3,18- one) dihydroxyestra-1,3,5, (10)­ 11ß-Hydroxyandrosterone TMSi, trien-17-one) (see 3a,l1ß-dihydroxy-5a• 17 ß-Hydroxy-17 a-ethinylandrost-4- androstan-17-one di-[trimethyl­ en-3-one,104 sily1 ether] ) 11ß-Hydroxyetiocholanolone, (see 17 a-Hydroxy corticoids 3a,11ß-dihydroxy-5ß-androstan- conversion to 17-ketosteroids, 209 17-one) bis-methylene dioxyderivatives, 11ß-Hydroxyetiocholanolone TMSi, 209 (see 3a, 11ß-dihydroxy-5ß• 6ß-Hydroxycortisol, (see 6ß,l1ß,17a, androstan-17-one di-[trimethyl 21-tetrahydroxypregn-4-ene-3, sily1 ether] ) 20-dione) 17 ß-Hydroxy-17 a-methylandrost-4- 20ß-Hydroxycortisol, (see llß,17a, en-3-one, 104 20 ß ,21-tetrahydroxypregn -4-en- 17 ß-Hydroxy-19-nor-androst-4-en- 3-one) 3-one,194 Subject Index 285

3ß-Hydroxy-5a-pregnane-11 ,20-dione, 17a-Hydroxyprogesterone, (see 110,124 17 a-hydroxypregn-4-ene-3,20- 21-Hydroxy-5ß-pregnan-3,20-dione, dione) 124 20a-Hydroxyprogesterone, (see 3a-Hydroxy-5ß-pregnan-20-one, 20a-hydroxypregn-4-en-3-one) 110,132,156,169,171 20ß-Hydroxyprogesterone, (see 3a-Hydroxy-5ß-pregnan-20-one 20ß-hydroxypregn-4-en-3-one) acetate, 133 20ß-Hydroxyprogesterone acetate, 3ß-Hydroxy-5a-pregnan-20-one, (see 20ß-hydroxypregn-4-en-3- 110,117,124,127 one acetate) 3ß-Hydroxy-5ß-pregnan-20-one, 20ß-Hydroxyprogesterone chloroace- 63,110,117,130,139 tate, (see 20ß-hydroxypregn-4- 20 ß-Hydroxy-5a- pregnan-3-one, en-3-one chloroacetate) 110,124,127 17a-Hydroxypregnanolone, (see I 3a,17a-dihydroxy-5ß-pregnan- Injection systems, 21 20-one) breakdown of sam pIe in, 79 17 a-Hydroxypregnanolone acetate, effect of injection port design, 22 (see 3a,17 a-dihydroxy-5ß• solid, 26 pregnan-20-one 3-acetate) syringe, 22 11a-Hydroxypregn-4-ene-3,20-dione, Tenney-Harris,25 105,111 11ß-Hydroxypregn-4-ene-3,20-dione, Infrared spectrometry, 52 111,209 Instrumentation, 19 17a-Hydroxypregn-4-ene-20-dione, differential flow controller, 20 105,156,181,192,193,194,201 gas controls, 20 21-Hydroxypregn -4-ene-3 ,20-dione, 209 K 17 a-Hydroxypregnenolone, (see 11-Ketoandrosterone, (see 3ß,17 a-dihydroxy-pregn-5-en-20- 3a-hydroxy-5a-androstane-11,17- one) dione) 3ß-Hydroxypregn-5-en-20-one, 111 16-Ketoestradiol, (see 3,17 ß• 11a-Hydroxy-5a-pregn-2-en-20-one, dihydroxyestra-1,3,5 (10) -trien- 111 16-one) 20a-Hydroxypregn-4-en-3-one, 16-Ketoestrone, (see 3-hydroxyestra- 104,129,156,194 1,3,5 (10) -trien-15,17-dione) 20ß-Hydroxypregn-4-en-3-one, 11-Ketoetiocholanolone, (see 104,129,156,194 3a-hydroxy-5ß-androstane-11, 20 ß -Hydroxypregn -4-en-3-one 17-dione) acetate, 133 11-Ketoetiocholanolone TMSi, (see 20ß-Hydroxypregn-4-en-3-one 3a-hydroxy-5ß-androstane-11, chloroacetate, 154, 157 17 -dione trimethylsilyl ether) 3ß-Hydroxypregn-5-en-20-one, 11-Ketoprogesterone, (see pregn-4- 132,156,169,181,192,193,194 ene-3,11,20-trione) 3ß-Hydroxypregn-5-en-20-one 17-Ketosteroids, 63, 64, 165 acetate, 133 determination of, 167, 176 21-Hydroxypregn-4-ene-3,11,20- hydrolysis of conjugates in urine, trione, 194, 209 173 11a-Hydroxyprogesterone, (see urinary (C1902), 170 11a-hydroxypregn-4-ene-3,20- dione) M 11ßHydroxyprogesterone, (see Mass spectrometry, 53 11ß-hydroxypregn-4-ene-3,20- connection to gas chromatograph, dione) 54 286 Subject Index

high resolution, 54 N low resolution, 54 19-Norandrostenedione, (see 19- 3a-Methoxy-7a,12a-dihydroxy-5ß• norandrost-4-ene-3,17-dione) cholanic acid methyl ester, 116 19-Norandrost-4-ene-3,17-dione, 124 3a-Methoxy-7a,12a-dihydroxy-5 ß• 19-Nortestosterone, (see 17ß• chol-7-enic acid methyl ester, 116 hydroxy-19-norandrost-4-en-3- 3a-Methoxy-7a,12a-dihydroxy-5ß• one) chol-9-enic acid methyl ester, 116 Nuclear magnetic resonance 3a-Methoxy-7a,12a-dihydroxy-5 ß• spectrometry (NMR), 53 chola-7,9-dienic acid methyl ester, 116 o 2-Methoxyestriol, (see estra-1,3,5 Optical rotatory dispersion, 52 (10) -triene-2,3,16a,17 ß-tetrol 2-methyl ether) P 2-Methoxyestrone, (see 2,3-dihy­ Partition coefficient, definition, 7 droxy-1,3,5 (10) -trien-17-one Peak distortion, 2-methyl ether) effect of feed volume on, 10 3a-Methoxymethyl cholanate, (see due to non-linearity, 11 3a-methoxy-7a,l2a-dihydroxy- due to support adsorptivity, 12 5ß-cholanic acid methyl ester) Peak height measurement, 56 3a-Methoxymethyl-~ 7-cholenate, Peak area measurement, 56 (see 3a"methoxy-7a,12a­ electronic integration, 56 dihydroxy-5ß-chol-7-enic acid Plate theory, 8 methyl ester) Prednisone, (see 17a,21-dihydroxy- 3a-Methoxymethyl-~9-cholenate, pregna-1,4-dione-3,11,20-trione) (see 3a-methoxy-7a,12a­ Pregnanes, 61 dihydroxy-5ß-chol-9-enic acid 5a-Pregnane,110, 112, 116 methyl ester) 5ß-Pregnane, 110, 112, 117 3a-Methoxymethyl-~ 7,9-choladienate, Pregnanediol, (see 5ß-pregnane- (see 3a-methoxy-7a,12a­ 3a,20a-diol) dihydroxy-5ß-chola-7,9-dienic 5a-Pregnane-3a,20a-diol, 127, 130, acid methyl ester) 132,139,169 17a-Methyl androst-5-ene-3ß,17 ß• 5a-Pregnane-3ß,20a-diol, 108, 110, diol,124 124,139 Methyl cholanate, (see 3a,7a,12a,­ 5a-Pregnane-3ß,20ß-diol, 108, 110, trihydroxy-5ß-cholanic acid 124,127,139 methyl ester) 5ß-Pregnane-3a,20a-diol, 63, 110, 129, Methl ~7-cholenate, (see 3a,7a,12a­ 130, 131, 132, 133, 136, 137, 138, trihydroxy-5ß-chol-7-enic acid 139, 146, 147, 148, 156, 169, 170, methyl ester) 171, 173, 190, 192, 194, 241, 242, Methyl ~9-cholenate (see 3a,7a,12a­ 248 trihydroxy-5ß-chol-9-enic acid analysis, 134 methyl ester) determination in urine, 131, 133, 16a-Methyl pregn-4-en~3,20-dione, 147,148 105 effect of ACTH, 145 16ß-Methylpregn-4-ene-3,20-dione, excretion, 142, 143, 144, 145 105 excretion during normal men­ 16a-Methyl-5a-pregn-2-ene-11,20- strual cycle, 248 dione,l11 hydrolysis of glucosiduronate, 16a-Methyl-5a-pregn-2-en-20-one, 146 111 quantitative estimation, 134 Microliter syringe, 24 recovery, added to urine, 141 Mobile phase, definition, 3 urinary excretion comparison, 135 Subject Index 287

5ß-Pregnane-3a,20ß-diol, 110 5a-Pregnane-3,11,20-trione, 105, 108, 5ß-Pregnane-3ß,20a-diol,139 110,124, 127 Pregnanediol diacetate, (see 5ß-Pregnane-3,11,20-trione, 105, 111, 5ß-pregnane-30a,20a-diol 117 diacetate) Pregnenediol, (see pregn-5-ene-3ß, 5a-Pregnane-3a,20a-diol diacetate, 20a-diol) 133 Pregn-5-ene-3ß,20ß-diol,139 5a-Pregnan-3ß,20a-diol diacetate, Pregn-5-ene-3ß,20a-diol, 132, 139, 169 108 5a-Pregn-2-ene-11,20-dione, 111 5a-Pregnan-3ß,20ß-diol diacetate, Pregn-4-ene-3,20-dione, 63, 104, 111, 108 124,129,152,156,180,181,194, 5ß-Pregnane-3a,20a-diol diacetate, 209 81,133,147 determination of, 150 5ß-Pregnane-3a,20a-diol diacetate, determination in plasma, 157, 159 infrared spectrum, 140 glc of, 153 5a-Pregnane-3ß,7a-diol dimethyl 5a-Pregn-2-en-20-one, 111 ether, 116, 120 Pregn-4-en-3-one, 111 5a-Pregnane-3ß, 7ß-diol dimethyl Pregnenolone, (see 3ß-hydroxypregn- ether, 116, 120 5-en-20-one) 5a-Pregnane-3,20-dione, 108, 110, Pregnenolone acetate, (see 3ß-hy• 124,127 droxypregn-5-en-20-one acetate) 5ß-Pregnane-3,20-dione, 110, 117, Pregnenetriol, (see pregn-5-ene- 124,127 3ß,17a,20a-triol) 5a-Pregnan-3a-ol, 110 Pregn-5-ene-3ß,17a,20a-triol, 5ß-Pregnan-3a-ol, 110, 117 132,169,202,203 5a-Pregnan-3ß-ol, 110 Pregn-5-ene-3a,16a,20a-triol,203 5ß-Pregnan-3ß-ol, 110, 117 Pregn-5-ene-3ß, 17 a,20ß-triol, 203 5a-Pregnan-11ß-ol,105 Pregn-5-ene-3ß ,17 a,20a-triol 5a-Pregnan-20a-ol, 110, 112 3,17,20-tri-[trimethylsilyl 5a-Pregnan-20ß-ol, 110, 112 ether], 205 Pregnanolone, (see 3a-hydroxy-5ß- Pregn-4-ene-3,11,20-trione, 105, 194 pregnan-20-one) Pregn-4-ene-3,6,20-trione, 111 Pregnanolone acetate, (see 3a­ Pressure drop, correction factor, 6 hydroxy-5ß-pregnan-20-one Progesterone, (see pregn-4-ene-3,20- acetate) dione) 5a-Pregnan-3-one, 110 5a-Pregnan-11-one,105,110 Q 5a-Pregnan-20-one,110,112 Quantitative analysis, 55 5ß-Pregnan-20-one, 110,112, 117 accuracy,55 5ß-Pregnane-3a,l1ß,17a,20a,21- precision, 55 pentol,63,190,207,208,210, peak measurement, 55 211,213,220 partially separated peaks, 57 5ß-Pregnane-3a,l1ß ,17 a,20ß,21- calibration, 58 pentol,208, 210, 211, 220 Qualitative analysis, 49, 50 Pregnanetriol, (see 5ß-pregnane- 3a,17a,20a-triol) R 5a-Pregnane-3a,6a,20a-triol, 65 Raoult's law, 11 5ß-Pregnane-3a,17a,20a-triol, 63, 64, Retention time 132, 169, 170, 192, 203 definition, 5 determination in urine, 201, 203 effect of stubstituent groups on, 5ß-Pregnane-3a,17a,20a-triol 102 tri acetate, 113 measurement of, 12 5ß-Pregnane-3a,17a,20a-triol relation to structure, 99 3,17,20-[trimethylsilyl Recorders, 46 ether], 205 288 Subject Index

Rechstein's "Substanz S" (see 17a,21- Tetrahydrocortexolone, (see dihydroxypregn-4-ene-3,20- 3a,17a,21-trihydroxy-5ß• dione) pregnan-20-one) Retention volume Tetrahydrocortisol, (see 3a,l1ß,17 a, adjusted,6 21-tetrahydroxy-5 ß-pregnan-20- definition, 5 one) effect of temperature on, 7 Tetrahydrocortisone, (see 3a,17 a,21- net,6 trihydroxy-5ß -pregnan-ll ,20- specific,6 dione) Relative retention, 7 Tetrahydro "Substanz S", (see effect of temperature on, 8 3a17 a,21-trihydroxy-5ß-pregnan- Resolution 20-one) definition, 10 3a,17a,20a,21-Tetrahydroxy-5ß• number of plates required, 10 pregnan-11-one, 63, 190, 207, Ring D a-ketols, 243 208,210,211,213,220 determination in pregnancy urine, 261 3a,17 a,20ß,21-Tetrahydroxy-5ß• pregnan-ll-one, 208,210, 211, S 220 Solanidan-3ß-ol, 124 3a,l1ß ,17 a,21-Tetrahydroxy-5a­ Stigmastane, 116 pregnan-20-one, 190,208 Standard compounds, purity of, 91 3a,l1ß,17a,21-Tetrahydroxy-5ß• Solvents pregnan-20-one, 190, 208, 213, purity of, 91 216 retention times of impurities in, 92 6ß,l1ß,17a,21-Tetrahydroxypregn- SampIe purification, 97 4-ene-3,20-dione, 213, 216 Solid support, 70 l1ß,17 a,20ß,21-Tetrahydroxypregn- adsorptivity, 70 4-en-3-one,213,216 coating,73 mechanical strength, 72 Theoretical plates, definition, 8 Tigogenin, 124 particIe size, 71 pore size, 70 Trapping methods, 49, 51 Stationary phase 3a, 7a,12a- Trihydroxy-5ß-cholanic definition, 3 acid methyl ester, 116 3a, 7a,12a- Trihydroxy-5ß-chol-7-enic selectivity, 13, 73 acid methyl ester, 116 stability, 73 3a, 7a,12a- Trihydroxy-5ß-chol-9-enic volatility,72 "Substanz S" (see 17a,21-dihydroxy­ acid methyl ester, 116 pregn -4-ene-3-20-dione) 3ß,IIß,21-Trihydroxy-20-oxo-5ß• pregnan-18-al, 65 T 3a,17 a,21-Trihydroxy-5ß-pregnan-ll, Temperature controllers, 29 20-dione,190,207,208,213,216 Testosterone, (see 17 ß-hydroxyan- 3a,17 a,21-Trihydroxy-5ß-pregnan- drost-4-en-3-one) 20-one, 190,208 Testosterone acetate, (see 17ß• 11ß,17 a,21-Trihydroxypregn-4-ene- hydroxyandrost-4-en-3-one 3,20-dione, 180, 190, 192, 194,208, acetate) 209, 213, 216, 241, 242 Testosterone chloroacetate, (see 17 a,20 ß ,21-Trihydroxypregn-4-ene-3, 17 ß-hydroxyandrost-4-en-3-one 20-dione, 213, 216 chloroacetate) 6ß ,17 a,21-Trihydroxypregn-4-ene-3, al-Testosterone, (see 17ß-hydroxyan• 1l,20-trione, 213 drosta-l,4-dien-3-one) Tetrahydroaldosterone, (see U 3ß,IIß,21-trihydroxy-20-oxo- Ultraviolet absorption spectrometry, 5ß-pregnan-18-al) 51