Curriculum vitae Kentaro Inoue

KENTARO INOUE Texas A&M Natural Resources Institute Texas A&M University Dallas, Texas 75252, U.S.A. 469-866-5894; [email protected]

EDUCATION 2015 Ph.D. Zoology with certificate in Ecology, Miami University, Oxford, Ohio, USA Dissertation: A comprehensive approach to conservation biology: from population genetics to extinction risk assessment for two of freshwater mussels (Advisor: David J. Berg) 2009 M.S. Environmental Sciences, Arkansas State University, Jonesboro, Arkansas, USA Thesis: Molecular phylogenetic, morphometric, and life history analyses of special concern freshwater mussels: jacksoniana (Frierson, 1912) and its closest congener arkansasensis (Lea, 1862) (Advisors: Alan D. Christian and Tanja McKay) 2007 B.S. Wildlife Ecology and Management, Arkansas State University, Jonesboro, Arkansas, USA. (Advisor: James C. Bednarz)

PROFESSIONAL APPOINTMENTS / EMPLOYMENT 2016–present Assistant Research Scientist, Institute of Renewable Natural Resources, Texas A&M University, Dallas, Texas, USA 2015–2016 Postdoctoral Researcher, Technical University of Munich, Freising, Germany (Advisor: Jürgen Geist)

RESEARCH INTERESTS Aquatic Ecosystems Conservation Biology / Genetics Biodiversity Conservation Evolutionary Biology Freshwater Molluscs Global Climate Change Landscape Genetics Macroinvertebrates Phylogenetics / Phylogeography Population Genetics / Genomics Speciation Species Distribution Models

PUBLICATIONS (** indicates mentored undergraduate student) Peer Reviewed In press **Adams NE, Inoue K, Berg DJ, Keane B, Solomon NG (2017) Range-wide microsatellite analysis of the genetic population structure of prairie voles (Microtus ochrogaster). American Midland Naturalist, 177, 183–199.

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2017 Inoue K, Stoeckl K, Geist J (2017) Joint species models reveal the effects of environment on community assemblage of freshwater mussels and fishes in European rivers. Diversity and Distributions, 23, 284–296. 2017 Inoue K, Berg DJ (2017) Predicting the effects of climate change on population connectivity and genetic diversity of an imperiled freshwater mussel, Cumberlandia monodonta (: Margaritiferidae), in riverine systems. Global Change Biology, 23, 94–107. 2017 Youngquist MB, Inoue K, Berg DJ, Boone MD (2017) Effects of land use on population presence and genetic structure of an amphibian in an agricultural landscape. Landscape Ecology, 32, 147–162. 2016 **Holste DR, Inoue K, Lang BK, Berg DJ (2016) Identification of microsatellite loci and examination of genetic structure for the endangered springsnails Juturnia kosteri and Pyrgulopsis roswellensis in the Chihuahuan Desert. Aquatic Conservation: Marine and Freshwater Ecosystems, 26, 715–723. 2015 Inoue K, Lang BK, Berg DJ (2015) Past climate change drives current genetic structure of an endangered freshwater mussel species. Molecular Ecology, 24, 1910–1926. 2014 Inoue K, Monroe EM, Elderkin CL, Berg DJ (2014) Phylogeographic and population genetic analyses reveal Pleistocene isolation followed by high gene flow in a wide-ranging but endangered freshwater mussel. Heredity, 112, 282–290. 2014 Inoue K, Levine TD, Lang BK, Berg DJ (2014) Long-term mark-and-recapture study of a freshwater mussel reveals patterns of habitat use and an association between survival and river discharge. Freshwater Biology, 59, 1872–1883. 2014 Inoue K, **McQueen AL, Harris JL, Berg DJ (2014) Molecular phylogeny and morphological variation reveal recent speciation in freshwater mussels of the genera Arcidens and Arkansia (Bivalvia: ). Biological Journal of the Linnean Society, 112, 535–545. 2014 **Morningstar CR, Inoue K, Sei M, Lang BK, Berg DJ (2014) Quantifying morphological and genetic variation of sympatric populations to guide conservation of endangered, micro- endemic springsnails. Aquatic Conservation: Marine and Freshwater Ecosystems, 24, 536– 545. 2014 **Ansah KN, Inoue K, Lang BK, Berg DJ (2014) Identification and characterization of 12 microsatellite loci for Physa in the Chihuahuan Desert. Conservation Genetics Resources, 6, 769–771. 2013 Inoue K, Hayes DM, Harris JL, Christian AD (2013) Phylogenetic and morphometric analyses reveal ecophenotypic plasticity in freshwater mussels: and (Bivalvia: Unionidae). Ecology and Evolution, 3, 2670–2683. 2013 Inoue K, Lang BK, Berg DJ (2013) Development and characterization of 20 polymorphic microsatellite markers for the Texas hornshell, Popenaias popeii (Bivalvia: Unionidae), through next-generation sequencing. Conservation Genetics Resources, 5, 195–198. 2011 Inoue K, Moyer GR, Williams A, Monroe EM, Berg DJ (2011) Isolation and characterization of 17 polymorphic microsatellite loci in the spectaclecase, Cumberlandia monodonta (Bivalvia: Margaritiferidae). Conservation Genetics Resources, 3, 57–60.

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Under Revision / Review In revision Inoue K, Lang BK, Berg DJ. Integrating population viability analysis and genetic assessments into endangered species recovery plans. Submitted to Biological Conservation. In revision Hayes DM, Inoue K, Minton RL. Names change but the story remains the same: more polyphyletic pleurocerids. Submitted to Journal of Molluscan Studies. In review Inoue K, Pohl AL, Makiri S, Lang BK, Berg DJ. Use of species delimitation approaches to assess biodiversity in freshwater planarians (Platyhelminthes: Tricladida) from desert springs. Submitted to Zoologica Scripta. In review **Adams NE, Inoue K, Seidel RA, Lang BK, Berg DJ. Isolation drives increased diversification rates in freshwater amphipods. Submitted to Molecular Phylogenetics and Evolution. In review **Morningstar CR, Inoue K, Lang BK, Berg DJ. A comprehensive examination of the species status of Stagnicola caperata (Say, 1829) in the southwest United States. Submitted to Journal of Molluscan Studies. In Preparation Inoue K, **Taynor KN, Lang BK, Berg DJ. Phylogenetic analyses and DNA barcoding reveal hyper-variable mitochondrial sequences in Physa spp. (Gastropoda: Physidae) in the southwest United States. To be submitted to Molecular Phylogenetics and Evolution. **Willams TJ, Inoue K, Lang BK, Berg DJ. Evolutionary delineation and cryptic speciation of Hyalella spp. in the Chihuahuan Desert. To be submitted to Freshwater Science. Popular Articles 2008 Inoue K. Intruder can wreak havoc on environment. The Jonesboro Sun. 29 June. 2007 Inoue K. Tiny freshwater mussels have huge importance. The Jonesboro Sun. 4 November.

FELLOWSHIPS AND SCHOLARSHIPS 2015 TUM University Foundation Fellowship. Technical University of Munich, Munich, Germany 2014 Dissertation Scholarship. Miami University, Oxford, Ohio, USA.

GRANTS AND AWARDS Graduate Awards 2013 Malacological Society of London, Research Grant. Utilization of environmental DNA to detect endangered mussel populations in the Southwest U.S. £1,499 2012 Malacological Society of London, Travel Grant. Attendance at the International Meeting on the Biology & Conservation of Freshwater Bivalves. Bragança, Portugal. £250 2011 Student Award for Best Poster, Honorable Mention. The 7th Biennial Freshwater Mollusk Conservation Society Symposium. “Phylogenetic and morphometric analyses reveal phenotypic plasticity in freshwater mussels: synonymy of Villosa arkansasensis and Obovaria jacksoniana”

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2011 Freshwater Mollusk Conservation Society, Travel Award. Attendance at the 7th Biennial Freshwater Mollusk Conservation Society Symposium. Louisville, Kentucky. $250 2008 Conchologists of America, COA Grants to Malacology. Conservation genetics of the critically imperiled freshwater snail, Leptoxis arkansensis (Hinkley, 1915) (Gastropoda: Pleuroceridae). $1,060 Internal Awards 2014 Miami University Graduate Student Achievement Award. $350 2011 Miami University Graduate Student Achievement Award. $300

PRESENTATIONS (presenter is underlined; ** indicates mentored undergraduate student) Oral Presentations 2016 Inoue K, Berg DJ. Use of GenAlEx to describe genetic variation and structure. 2016 FMCS Workshop: Population Genetics And Freshwater Mollusk Conservation. Shepherdstown, West Virginia. February 16–19, 2016 2015 **Williams TJ, Inoue K, Lang BK, Berg DJ. Evolutionary delineation and cryptic speciation of Hyalella spp. in the Chihuahuan Desert. Society for Freshwater Science 2015 Annual Meeting. Milwaukee, Wisconsin. May 17–21, 2015. 2015 Inoue K, Berg DJ. Predicting the effect of climate change on population connectivity and genetic diversity in riverine systems: a case study of Cumberlandia monodonta (Bivalvia: Margaritiferidae). The 9th Biennial Freshwater Mollusk Conservation Society Symposium. St. Charles, Missouri. March 22–26, 2015. 2015 Walters AD, Inoue K, Harris JL, Berg DJ. Niche differentiation in evolutionary lineages of a freshwater mussel species complex. The 9th Biennial Freshwater Mollusk Conservation Society Symposium. St. Charles, Missouri. March 22–26, 2015. 2014 Inoue K, Levine TD, Lang BK, Berg DJ. Long-term mark-and-recapture study of a freshwater mussel reveals patterns of habitat use and an association between survival and river discharge. Joint Aquatic Sciences Meeting 2014. Portland, Oregon. May 18–23, 2014. 2014 Walters AD, Inoue K, Harris JL, Berg DJ. Niche overlap and divergence time are negatively correlated in a freshwater mussel species complex. Joint Aquatic Sciences Meeting 2014. Portland, Oregon. May 18–23, 2014. 2013 Inoue K, Lang BK, Berg DJ. Population genetic analyses reveal recent isolation of Popenaias popeii, in the southwest United States. 8th Biennial Freshwater Mollusk Conservation Society Symposium. Guntersville, Alabama. March 10–14, 2013. 2013 Inoue K, Monroe EM, Elderkin CL, Berg DJ. Phylogeographic and population genetic analyses reveal multiple lineages and post-glacial expansion in a wide-ranging endangered mussel, Cumberlandia monodonta (say 1829). 8th Biennial Freshwater Mollusk Conservation Society Symposium. Guntersville, Alabama. March 10–14, 2013. 2012 Inoue K, Levine TD, Lang BK, Berg DJ. Use of distance sampling to estimate population size and density of the Texas hornshell (Popenaias popeii, Lea 1857) in the Black River, NM,

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USA. International Meeting Biology & Conservation of Freshwater Bivalves. Bragança, Portugal. September 4–7, 2012. 2012 Berg DJ, Inoue K, **Adams NE, Pohl AL, **Sugita MG, Sei M, Lang BK. Comparative phylogeography of desert spring invertebrates suggests complex patterns of evolution and cause for conservation concern. Society for Freshwater Science 60th Annual Meeting. Louisville, Kentucky. May 20–24, 2012. 2012 Inoue K, Levine TD, Lang BK, Berg DJ. Use of distance sampling to estimate population size and density of the Texas hornshell (Popenaias popeii, Lea 1857) in the Black River, NM, USA. Society for Freshwater Science 60th Annual Meeting. Louisville, Kentucky. May 20–24, 2012. 2009 Inoue K, Harris JL, Hayes DH, Christian AD. Molecular phylogenetic and geometrics analyses on Obovaria jacksoniana (Frierson, 1912) and Villosa arkansasensis (Lea, 1862) (Bivalvia: Unionidae). 6th Biennial Freshwater Mollusk Conservation Society Symposium. Baltimore, Maryland. April 19–24, 2009. 2009 Harris JL, Hoeh WR, Posey II WR, Davidson CL, Oetker SR, Farris JL, Serb JM, Inoue K, Hayes DM, Gordon ME, Christian AD. Species richness and conservation status of Arkansas’ unionid bivalves. 6th Biennial Freshwater Mollusk Conservation Society Symposium. Baltimore, Maryland. April 19–24, 2009. Poster Presentations 2016 Walters AD, Inoue K, Harris JL, Berg DJ. Niche and lineage diversification in a freshwater mussel species complex. 2016 FMCS Workshop: Population Genetics And Freshwater Mollusk Conservation. Shepherdstown, West Virginia. February 16–19, 2016 2015 **Morningstar CM, Inoue K, Lang BK, Berg DJ. An investigation of cryptic speciation: examining the genetic, morphological and soft-tissue variation of five populations of the freshwater wrinkled marshsnail (Stagnicola caperata). The 9th Biennial Freshwater Mollusk Conservation Society Symposium. St. Charles, Missouri. March 22–26, 2015. 2014 **Morningstar CM, Inoue K, Lang BK, Berg DJ. An investigation of cryptic speciation: morphological variation and population structure of the gastropod Staginocla caperata at differing elevations. Joint Aquatic Sciences Meeting 2014. Portland, Oregon. May 18–23, 2014. 2014 **Williams TJ, Inoue K, Lang BK, Berg DJ. Genetic variation and cryptic speciation of the Chihuahuan Desert amphipod (Hyalella spp.). Joint Aquatic Sciences Meeting 2014. Portland, Oregon. May 18–23, 2014. 2014 Inoue K, **McQueen AL, Harris JL, Berg DJ. Molecular phylogenetics and morphological variation reveal recent speciation in freshwater mussels (Bivalvia: Unionidae: Arcidens & Arkansia). Freshwater Mollusk Conservation Society 2014 Workshop. Portland, Maine. April 24–25, 2014. 2014 Inoue K, **McQueen AL, Harris JL, Berg DJ. Molecular phylogenetics and morphological variation reveal recent speciation in freshwater mussels (Bivalvia: Unionidae: Arcidens & Arkansia). Midwest Ecology & Evolution Conference. Dayton, Ohio. March 1–2, 2014.

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2014 **Morningstar CM, Inoue K, Lang BK, Berg DJ. An investigation of cryptic speciation: morphological variation and population structure of the gastropod Staginocla caperata at differing elevations. Midwest Ecology & Evolution Conference. Dayton, Ohio. March 1–2, 2014. 2014 **Williams TJ, Inoue K, Lang BK, Berg DJ. Genetic variation and cryptic speciation of the Chihuahuan desert amphipod Hyalella spp. Midwest Ecology & Evolution Conference. Dayton, Ohio. March 1–2, 2014. 2013 **Ansah K, Inoue K, Sei M, Lang BK, Berg DJ. Population genetic analyses reveal potential micro-endemism in “Physa acuta” in a Chihuahuan desert spring system. 8th Biennial Freshwater Mollusk Conservation Society Symposium. Guntersville, Alabama. March 10– 14, 2013. 2013 **Morningstar CR, Inoue K, Lang BK, Berg DJ. Characterizing genetic variability and population structure of the freshwater wrinkled marshsnail (Stagnicola caperata) in high and low altitude populations. 8th Biennial Freshwater Mollusk Conservation Society Symposium. Guntersville, Alabama. March 10–14, 2013. 2012 **Morningstar CR, Inoue K, Sei M, Lang BK, Berg DJ. Genetic variation of the endangered springsnails Pyrgulopsis roswellensis and Juturnia kosteri endemic to the Chihuahuan Desert. Society for Freshwater Science 60th Annual Meeting. Louisville, Kentucky. May 20– 24, 2012. 2011 **Adams NE, Inoue K, Seidel RA, Sei M, Lang BK, Berg DJ. DNA Barcoding reveals two diversification of Gammarus amphipods (Crustacea: Gammaridae) in the Chihuahuan Desert springs. North American Benthological Society 59th Annual Meeting. Providence, Rhode Island. May 22–26, 2011. 2011 Inoue K, Hayes DM, Harris JH, Christian AD. Phylogenetic and morphometric analyses reveal phenotypic plasticity in freshwater mussels: synonymy of Villosa arkansasensis and Obovaria jacksoniana. 7th Biennial Freshwater Mollusk Conservation Society Symposium. Louisville, Kentucky. April 11–15, 2011. 2011 **Sugita MG, Inoue K, Sei M, Lang BK, Berg DJ. Genetic diversity and cryptic speciation of Physa species (Gastropoda: Physidae) in the Chihuahuan Desert. 7th Biennial Freshwater Mollusk Conservation Society Symposium. Louisville, Kentucky. April 11–15, 2011. 2011 Hayes DM, Inoue K, Serb JM, Harris JL, Christian AD. Western pigtoes: a preliminary phylogenetic analysis of Fusconaia and Pleurobema (Unionidae) from west of the Mississippi River. 7th Biennial Freshwater Mollusk Conservation Society Symposium. Louisville, Kentucky. April 11–15, 2011. 2009 Hayes DM, Inoue K, Minton RL, Christian AD. Molecular systematics of Interior Highland Pleuroceridae (Gastropoda). 6th Biennial Freshwater Mollusk Conservation Society Symposium. Baltimore, Maryland. April 19–24, 2009. 2008 Inoue K, Harris JL, Posey II WR, Christian AD. Phylogenetic and morphometric analyses on the special concern mussel species: southern hickorynut, Obovaria jacksoniana (Frierson, 1912) and Ouachita creekshell, Villosa arkansasensis (Lea, 1862). North American Benthological Society 56th Annual Meeting. Salt Lake City, Utah. May 25–30, 2008.

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2008 Inoue K, Harris JL, Posey II WR, Christian AD. Phylogenetic analysis on the special concern mussel species: southern hickorynut, Obovaria jacksoniana (Frierson, 1912), and Ouachita creekshell, Villosa arkansasensis (Lea, 1862). The joint annual meeting of the Arkansas and Mississippi chapters American Fisheries Society. Tunica, Mississippi. February 20–22, 2008. 2007 Inoue K, Christian AD. Land use and freshwater mussel assemblage changes in the lower White River, Arkansas: a 1930 to 2002 perspective. Undergraduate Scholars Day 2007. Arkansas State University, Jonesboro, Arkansas. April 10, 2007. 2007 Inoue K, Harris JL, Christian AD. Land use and freshwater mussel assemblage changes in the lower White River, Arkansas: 1930 to 2002. 5th Biennial Freshwater Mollusk Conservation Society Symposium. Little Rock, Arkansas. March 11–16, 2007. 2006 Inoue K, Harris JL, Christian AD. Spatial patterns of a deltaic stream freshwater mussel (: Bivalvia) bed: a geostatistical approach. Undergraduate Scholars Day 2006. Arkansas State University, Jonesboro, Arkansas. April 11, 2006.

STUDENT SUPERVISED (* indicates graduate student; ** indicates undergraduate student) 2016–present *Feye Holzwarth (Technical University of Munich): Uses closed-population mark-and- recapture methods to determine detection probabilities and population sizes of freshwater mussels among multiple river sizes. 2016–present *Andreas Dobler (Technical University of Munich): Uses ecological niche modeling and gap analyses to evaluate the coverage of protection areas for two endangered freshwater mussel species in Bavaria, Germany. 2014–2015 **Kristina Taynor (Miami University): Helped with DNA sequencing on Physa spp. in the Chihuahuan Desert. 2012–2015 **Kofi Ansah (Miami University): Characterized novel microsatellite markers for Physa spp. in the Chihuahuan Desert. Presented research at the Freshwater Mollusk Conservation Society Symposium (March 2013). This research led to a co-authored manuscript published in Conservation Genetics Resources. 2012–2015 **Trevor Williams (Miami University): Investigated evolutionary delineation and cryptic speciation of freshwater amphipods (Hyalella spp.) in the Chihuahuan Desert. Presented research at the Midwest Ecology & Evolution Conference (March 2014), the Joint Aquatic Sciences Meeting 2014 (May 2014), and Society for Freshwater Science Annual Meeting (May 2015). This research will lead to a co-authored manuscript to be submitted to Freshwater Science. Received a doctoral fellowship from Brigham Young University. 2012–2014 **Danielle Holste (Miami University): Characterized novel microsatellite markers for two micro-endemic springsnails in the Chihuahuan Desert. This research led to a co-authored manuscript published in Aquatic Conservation: Marine and Freshwater Ecosystems. 2012 **Emily Robinson (Miami University): Helped with DNA sequencing and fragment analyses on freshwater mussel species. 2011–2015 **Cayla Morningstar (Miami University): Investigated taxonomic status of wrinkled marshsnail (Stagnicola caperata) in New Mexico and west Texas. Presented this research

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at the Freshwater Mollusk Conservation Society Symposium (March 2013), the Midwest Ecology & Evolution Conference (March 2014), and the Joint Aquatic Sciences Meeting 2014 (May 2014). This research will lead to a co-authored manuscript to be submitted to Southwestern Naturalist. Also investigated morphological and genetic variation of sympatric micro-endemic springsnails in the Chihuahuan Desert. Presented this research at Society for Freshwater Science Annual Meeting (May 2012). This research led to a co- authored manuscript published in Aquatic Conservation: Marine and Freshwater Ecosystems. Currently, a field technician at the Florida Fish & Wildlife Conservation Commission. 2011–2012 **Corrina DeLorenzo (Miami University): Helped characterize microsatellite markers for Physa spp. 2010–2013 **Nicole Adams (Miami University): Investigated evolutionary history and speciation of freshwater amphipods (Gammarus spp.) in the Chihuahuan Desert. Presented research at the North American Benthological Society Annual Meeting (May 2011). This research will lead to a co-authored manuscript to be submitted to the Proceedings of the Royal Society of London B. Currently in doctoral program at the University of Southern California. 2010–2012 **David Moulton (Miami University): Helped with DNA sequencing of Physa spp. in the Chihuahuan Desert. Presented research at the Miami University Undergraduate Research Forum (April 2012). Currently in doctoral program at Texas A&M University 2009–2013 **Alyssa McQueen (Miami University): Investigated species delineation of freshwater mussel species in the Mississippi River basin. Co-author on a peer-reviewed manuscript published in the Biological Journal of the Linnean Society. 2009–2012 **Megumi Sugita (Miami University): Helped with DNA sequencing of Physa spp. in the Chihuahuan Desert. Presented research at the Freshwater Mollusk Conservation Society Symposium (April 2011).

TEACHING 2014 Fundamentals of Ecology, Guest lecturer, Miami University 2009–2010 General Biology, Teaching Assistant, Miami University

SERVICE External Reviewer (23 articles from 18 journals) American Malacological Bulletin (1) Aquatic Conservation: Freshwater and Marine Ecosystems (1) Aquatic Invasions (2) Biochemical Systematics and Ecology (2) Biological Journal of the Linnean Society (2) Conservation Genetics (3) Contributions to Zoology (1) Freshwater Biology (1) Freshwater Mollusk Biology and Conservation (2)

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Hydrobiologia (1) Journal of Biogeography (1) Limnology (1) Marine Biology Research (1) Northeastern Naturalist (1) PeerJ (1) PLOS ONE (1) Systematics and Biodiversity (1) Zoological Journal of the Linnean Society (1) Professional Membership 2012–present Malacological Society of London 2008–present Society for Freshwater Science (formerly North American Benthological Society) 2007–present Freshwater Mollusk Conservation Society

CERTIFICATIONS Professional Association of Diving Instructors (PADI) Open Water Diver SCUBA Certification (Student No.0612071409)

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REFERENCES David J. Berg, Ph.D. Department of Biology, Miami University 1601 University Boulevard, Hamilton, Ohio 45011, USA +1-513-785-3246; [email protected]

Bruce J. Cochrane, Ph.D. Department of Biology, Miami University 700 E High Street, Oxford, Ohio 45056, USA +1-513-529-3734; [email protected]

John L. Harris, Ph.D. Department of Biological Sciences, Arkansas State University P.O. Box 599, State University, Arkansas 72467, USA +1-501-961-1419; [email protected]

David M. Hayes, Ph.D. Department of Biological Sciences, Eastern Kentucky University 521 Lancaster Avenue, Richmond, Kentucky 40475 +1-859-622-1016; [email protected]

Alan D. Christian, Ph.D. School for Environment, University of Massachusetts at Boston 100 Morrissey Boulevard, Boston, Massachusetts 02125, USA +1-617-287-6639; [email protected]

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