Portland State University PDXScholar Dissertations and Theses Dissertations and Theses 1-1-1992 Development of a Semi-synthetic Medium Supporting Adherent Growth in Coagulase-Negative Staphylococci Abbas Sadeghi Portland State University Follow this and additional works at: https://pdxscholar.library.pdx.edu/open_access_etds Let us know how access to this document benefits ou.y Recommended Citation Sadeghi, Abbas, "Development of a Semi-synthetic Medium Supporting Adherent Growth in Coagulase- Negative Staphylococci" (1992). Dissertations and Theses. Paper 13. https://doi.org/10.15760/etd.13 This Thesis is brought to you for free and open access. It has been accepted for inclusion in Dissertations and Theses by an authorized administrator of PDXScholar. Please contact us if we can make this document more accessible:
[email protected]. AN ABSTRACT OF THE THESIS OF Abbas Sadeghi for the Master of Science in Biology presented August 3, 1992. Title: Development of a Semi-synthetic Medium Supporting Adherent Growth in Coagulase-negative staphylococci. APPROVED BY TilE MEMBERS OF TilE THESIS COMMITTEE: Mary. L./Taylor, Ohair Richard Peterson Gordo~ , Kilg:"~~r - A semi-synthetic medium for use in determining adherent growth with Staphylococcus epidermidis and Staphylococcus saprophyticus was developed. Production of an adherent biofilm was dependent upon the presence of hematin in the growth medium. Clinical strains of Staphylococcus epidermidis were tested for production of an adherent biofilm in trypticase soy broth, the semi-synthetic medium and the hyperalimentary nutrient solution used in the neonatal hospital unit. An adherent biofilm was obtained when 2 Staphylococcus epidermidis was cultured m hematin supplemented hyperalimentary solution. Growth in the hyperalimentary nutrient solution diluted with fetal calf serum showed the same growth rate as when the nutrient solution was diluted with water.