Anti-Glycophorin a Antibody (Biotin) Product Number: AC12-0684-07

Anti-Glycophorin A Antibody (Biotin) Product Number: AC12-0684-07

Overview Host Species: Rabbit Clonality: Monoclonal (GYPA/1725R) Purity: Antibody is purified by Protein A. The antibody is then conjugated to biotin, also known as vitamin B7, vitamin H, or coenzyme R, which is a small molecule which has an extremely high affinity for avidin and streptavidin. Isotype: IgG Conjugate: Biotin Immunogen Type: Anti-glycophorin a antibody was raised against recombinant full-length human glycophorin A protein. Physical Characteristics Amount: 0.05 mg Concentration: 1 mg/ml Volume: 0.05 ml Buffer: Antibody is purified by Protein A. Prepared in 10mM PBS at 1.0mg/ml. Storage: Biotin conjugated antibody can be stored at 4?C for up to 18 months. For longer storage the conjugate can be stored at -20?C after adding 50% glycerol. Specificity Species Reactivity: Human Specificity: Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Target Information Accession Number(s): Swissprot: P02724 (Human) Gene ID Number(s): 434973 (Human); 654368 (Human) Alternative Names: AI853584; Blood group--MN locus; CD_antigen=CD235a; CD235a; GLPA_HUMAN; Glycophorin A (MNS blood group); Glycophorin A; Glycophorin A; included; Glycophorin-A; GlycophorinA; GPA; GPErik; GpMiIII; GPSAT; GYPA; GYPA; included; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); MN; MN sialoglycoprotein; MNS; PAS-2; PAS2; Sialoglycoprotein alpha Application Details Tested Applications: IHC, ICC, IF, FC Immunocytochemistry: Recommended starting concentration for immunocytochemistry is 0.25-0.5 ug/ml for 30 min at RT (staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Immunoflourescence: Recommended starting concentration for immunofluorescence is 0.5-1 ug/ml. Flow Cytometry: Recommended starting concentration for flow cytometry is 0.5-1 ug/million cells.

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