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18 SOIPA Società Italiana di Parassitologia Comune di Alghero SOCIETÀ ITALIANA DI PARASSITOLOGIA XXVII Congresso Nazionale MAPPE PARASSITOLOGICHE MAPPE Alghero 26-29 giugno 2012 Dipartimento di Medicina Veterinaria In copertina: Protoscolice di Echinococcus granulosus Università degli Studi di Sassari (Foto G. Garippa) MAPPE PARASSITOLOGICHE MAPPE MAPPE PARASSITOLOGICHE 18 Mappe Parassitologiche CONTENTS Series Editor Lettura Magistrale Giuseppe Cringoli Relazioni ai Simposi e Tavola Rotonda Copyrigth© 2012 by Giuseppe Cringoli Comunicazioni Scientifiche del XXVII Congresso Nazionale della Società Italiana di Parassitologia Registered office Veterinary Parasitology and Parasitic Diseases Alghero, 26-29 giugno 2012 Department of Pathology and Animal Health GIOVANNI GARIPPA - Nota editoriale . 3 Faculty of Veterinary Medicine University of Naples Federico II Via della Veterinaria, 1 Lettura Magistrale 80137 Naples - Italy EUGENIA TOGNOTTI - Americani, comunisti e zanzare . 7 Tel +39 081 2536283 e-mail: [email protected] website: www.parassitologia.unina.it Simposio 1 Epidemiologia della trichinellosi nell’uomo CREMOPAR e negli animali in italia e in europa Centro Regionale per il Monitoraggio delle Parassitosi Località Borgo Cioffi - Eboli (Sa) BRUSCHI F. - We can still learn something from trichinellosis: from a public health problem to a model for studying im- Tel./Fax +39 0828 347149 mune mediated diseases . 13 e-mail: [email protected] POZIO E. - Epidemiology of Trichinella infections in humans All rights reserved – printed in Italy and animals of Italy and Europe . 15 No part of this publication may be reproduced in any form or by any REINA D. - Epidemiological situation of trichinellosis in means, electronically, mechanically, by photocopying, recording or Spain . 16 otherwise, without the prior permission of the copyright owner. MARINCULIĆ A. - Trichinellosis is still the most important zoo- Printed and bound by Litografia Vigilante srl notic disease in Southeastern and Eastern Europe . 17 Via Nuova Poggioreale, 151 D - 80143 Naples - Italy Tel/Fax +39 081 5846611 - e-mail: [email protected] BANDINO E. - Updating on trichinellosis in Sardinia . 18 (continued) II Contents Mappe parassitologiche 18 RUOCCO L., FALCONE M.G., PRIMAVERA A., FERRI G. - Animal Simposio 4 Trichinellosis: Italian application of Reg. EC 2075/2005 As- Protozoi di interesse medico sessment and perspectives . 21 GOLDSCHMIDT P., DEGORGE S., DI CAVE D., CHAUMEIL C. - New test for the detection and molecular characterization of Simposio 2 Acanthamoeba . 47 Aggiornamenti sulla leishmaniosi e novità per il suo controllo HENRIQUEZ F.L., ROBERTS C.W. - Identification of novel inhi- bitors of Acanthamoeba species . 48 MIRÓ G. - Update on epidemiology and treatment of canine leishmaniosis . 25 MATTANA A., PINNA A., ERRE G., FIORI P.L., CAPPUCCINELLI P. - Biological characterization of free-living amoebae isolated BIANCIARDI P. - Miltefosine: a new and more modern treat- from cases of amoebic keratitis in Sassari . 49 ment for Canine Leishmaniasis . 27 CANO A., HENRIQUEZ F.L., MATTANA A., JAMES A., ROBERTS MCGAHIE D. - New developments in prevention – what do I C.W. - Acanthamoeba castellanii stimulates the produc- need to know about the new vaccine? . 32 tion of IL-6, IL-12 and TNF-alpha by murine macropha- ges . 50 OLIVA G., NIETO J., FOGLIA MANZILLO V., CAPPIELLO S., FIO- RENTINO E., DI MUCCIO T., SCALONE A., MORENO J., CHI- MELONI D., DI CAVE D., FIORI P.L., VISCOGLIOSI E. - Blasto- CHARRO C., BUTAUD T., GUEGAND L., MARTIN V., CUISINIER cystis sp.: a world yet to be discovered . 51 A.M., GUEGUEN S., CAÑAVATE C., GRADONI L. - Evidence for protection against active infection and disease progression in FIORI P.L., RAPPELLI P., DESSÌ D., DIAZ N., COCCO A.R., CUC- naïve dogs vaccinated with LiESP/QA-21 (CaniLeish®) ex- CURU M.A., MARGARITA V., SANCIU G., SANTONA S., HIRT R.P. posed to two consecutive Leishmania infantum transmission - Trichomonas vaginalis pathobiology . 55 seasons . 34 DEL CHIERICO F., CONCATO C., ROSSI DE GASPERIS M., RANNO S., DI PIETRANTONIO L., DI CRISTANZIANO V., BERRILLI F., DI Simposio 3 CAVE D., MURACA M., CALLEA F., PUTIGNANI L. - Genotyping La dicroceliosi negli animali da reddito: of Cryptosporidium spp. isolates from different Tanzania diagnosi, terapia e strategie di controllo areas . 58 PIERGILI FIORETTI D. - Dicrocoelium dendriticum: epidemio- CACCIÒ S.M., SANNELLA A.R., MANUALI E., TOSINI F., SENSI logical characteristics of a parasite of ruminants . 37 M., CROTTI D., POZIO E. - Pigs as natural hosts of Dienta- moeba fragilis genotypes found in humans . 59 JACQUIET P. - Dicrocoeliosis in ruminants: physiopathological aspects . 40 Gabrielli S., Girelli G., Vaia F., Santonicola M., Fakeri A., Cancrini G. - Trypanosoma cruzi in blood donors: a study started at the Umberto 1° teaching Policlinic in Rome . 60 CRINGOLI G. - Copromicroscopic diagnosis of dicrocoeliosis: what’s new? . 41 ROMI R., BOCCOLINI D., MENEGON M., GNESIVO C., PERELLI F., VALENTINI I.R., FABIANO F., PAGNOTTA F., SEVERINI F., DI LUCA M., TOMA L., SEVERINI C., CESARIO F., GRADONI L., REZZA G. ANTENUCCI P. - Control and treatment of Dicrocoelium den- - Sporadic cases of cryptic malaria (2009-2011) and the risk driticum in small ruminants . 44 of local vector-borne transmission in Italy . 61 (continued) Mappe parassitologiche 18 Contents III AVELLINO P., MAGLIACCA C., LOMBARDO F., BOVE M., BOR- VALHO L., MENDOZA DE GIVES P., SÁNCHEZ-ANDRADE R., PAZ- RUSO L., MODIANO D. - Development of a new molecular ap- SILVA A. - Control of strongyles in captive equines by using the proach for the diagnosis of Trichomonas vaginalis in its nematophagous fungus Duddingtonia flagrans . 75 natural human host . 62 BOSCO A., RINALDI L., MORGAN E.R., COLES G.C., CRINGOLI CACCIÒ S.M., SANNELLA A.R., MARIANO V., VALENTINI S., G. - The maintenance of anthelmintic efficacy in sheep and CARDETI C., BERTI F., TOSINI F., TONANZI D., POZIO E. - goats in southern Italy . 76 Zoonotic transmission of Cryptosporidium parvum in Italy caused by an uncommon genotype . 63 CASTAGNA G., SCONZA S., SEGHETTI M., TRAVERSA D., FRAN- GIPANE DI REGALBONO A., MARCER F., BERALDO P. - Efficacy DI CAVE D. - Pneumocystis jirovecii: genotypes and molecular of fenbendazole and moxidectin in horse stables with pre- analysis at mtLSU rRNA and DHPS loci . 64 vious history of resistance to benzimidazoles in cyathostomin populations . 77 DI FILIPPO E., TALIANI G., GABRIELLI S., TEMPERONI C., CAN- CRINI G. - Reactivation of Toxoplasma gondii in HIV-infected FALSONE L., NAPOLI E., GAGLIO G., BRIANTI E., GIANNETTO population from rural area of Zimbabwe . 65 S. - Milbemax® (milbemyicin oxime/praziquantel) flavored tablets in cats naturally infected by helminths: a clinical GABRIELLI S., MILADINOVIĆ TASIĆ N., OTAŠEVIĆ, MARJANOVIĆ trial. 78 G., CANCRINI G. - Visceral leishmaniosis/HIV co-infection: first case detected in Nis (southeastern Serbia), and diagno- FRANGIPANE DI REGALBONO A., MARCER F., SIGNORINI M., stic observations on further five Italian cases . 66 MIOTTO L., SORIOLO A., SOLARI BASANO F., TONDELLO L., DA- NESI P., PIETROBELLI M. - Effectiveness of Decoquinate on control of subclinical coccidiosis in naturally infected beef Tavola Rotonda cattle: preliminary results . 79 L’echinococcosi cistica in sardegna: quali possibilità di controllo? GRANDI G., KRAMER L.H., CALZOLARI A., QUARANTELLI A., RIGHI F. - Influence of a nutritional addition of Oregano es- LIGHTOWLERS M. - Evaluation of new control options for cy- sential oil (OEO) on Eimeria spp. infection prevalence and stic echinococcosis . 69 oocyst shedding dynamics in dairy cattle calves. 80 CRAIG P.S. - Historical and current perspectives for CE con- LIA R.P., DANTAS-TORRES F., MOTTA B., SCHNYDER M., SOLARI trol . 70 BASANO F., SCHIESSL B., MALLIA E., OTRANTO D. - Efficacy of milbemycin oxime/praziquantel tablets (Milbemax®- Novartis KERN P. - Human cystic echinococcosis: WHO-IWGE ultrasound Animal Health) against Thelazia callipaeda in naturally in- classification of cysts allows tailored clinical management . 71 fested dogs . 81 MESLIN F.X., MAGNINO S. - Neglected Zoonotic Diseases MANFREDI M.T., FRAQUELLI C., PICCOLINO BONIFORTI P., RIZZI (NZDs) with special focus on cystic echinococcosis . 72 R., ZANZANI S. - Effects of condensed tannin on natural coc- cidian infection in goat kids. 82 Sessione 1 MARANGI M., MORELLI V., PATI S., CAMARDA A., CAFIERO Terapia e farmacoresistenza M.A., GIANGASPERO A. - Acaricide residues in laying hens na- turally infested by red mite Dermanyssus gallinae . 83 ARIAS M.S., CAZAPAL-MONTEIRO C., VALDERRÁBANO E., MIGUÉ- LEZ S., ROIS J.L., LÓPEZ-ARELLANO M.E., MADEIRA DE CAR- PIÑEIRO P., SANCHÍS J., MIGUÉLEZ S., RODRÍGUEZ M.I., SUÁREZ (continued) IV Contents Mappe parassitologiche 18 J.L., CAZAPAL-MONTEIRO C., PAZ-SILVA A., ARIAS M.S., SÁN- T., GRAMICCIA M. - Sand fly-borne diseases: sand fly seasonal CHEZ-ANDRADE R. - Control of trematode infection can be im- dynamics and search for Leishmania and Phleboviruses in proved by means of soil fungi . 85 two different areas of Rome province, Italy . 95 POLLICINO P., RAMBOZZI L., MOLINAR MIN A.R., ROSSI L. - Ef- BONGIORNO G., BIANCHI R., PAPARCONE R., FOGLIA MANZILLO ficacy and safety of topical eprinomectin to control Myocop- V., OLIVA G., BARBATO A., GRADONI L. - Antifeeding activity tes musculinus infestation in mice . 86 of Azadirachta indica (Neem) extract against sand fly bites on dogs . 96 SANNA G., VARCASIA A., CARBONI S., PIPIA A.P., MANCA R., GENCHI C., SCALA A. -
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  • Leishmania LABCG1 and LABCG2 Transporters

    Leishmania LABCG1 and LABCG2 Transporters

    Manzano et al. Parasites & Vectors (2017) 10:267 DOI 10.1186/s13071-017-2198-1 RESEARCH Open Access Leishmania LABCG1 and LABCG2 transporters are involved in virulence and oxidative stress: functional linkage with autophagy José Ignacio Manzano1, Ana Perea1, David León-Guerrero1, Jenny Campos-Salinas1, Lucia Piacenza2, Santiago Castanys1*† and Francisco Gamarro1*† Abstract Background: The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylserine (PS), in virulence and in resistance to antimonials. However, the involvement of this transporter in other key biological processes has not been studied. Methods: To better understand the biological function of LABCG2 and its nearly identical tandem-repeated transporter LABCG1, we have generated Leishmania major null mutant parasites for both genes (ΔLABCG1-2). NBD-PS uptake, infectivity, metacyclogenesis, autophagy and thiols were measured. Results: Leishmania major ΔLABCG1-2 parasites present a reduction in NBD-PS uptake, infectivity and virulence. In addition, we have shown that ΔLABCG1-2 parasites in stationary phase growth underwent less metacyclogenesis and presented differences in the plasma membrane’s lipophosphoglycan composition. Considering that autophagy is an important process in terms of parasite virulence and cell differentiation, we have shown an autophagy defect in ΔLABCG1-2 parasites, detected by monitoring expression of the autophagosome marker RFP-ATG8. This defect correlates with increased levels of reactive oxygen species and higher non-protein thiol content in ΔLABCG1-2 parasites. HPLC analysis revealed that trypanothione and glutathione were the main molecules accumulated in these ΔLABCG1-2 parasites.
  • Review on Paramphistomosis

    Review on Paramphistomosis

    Advances in Biological Research 14 (4): 184-192, 2020 ISSN 1992-0067 © IDOSI Publications, 2020 DOI: 10.5829/idosi.abr.2020.184.192 Review on Paramphistomosis 12Adane Seifu Hotessa and Demelash Kalo Kanko 1Hawassa University, Revenue Generating PLC Farm, P.O. Box: 05, Hawassa, Ethiopia 2Gerese Woreda Livestock and Fishery Resource Office, Gamo Zone, SNNPR, Ethiopia Abstract: Paramphistomum is considered to be one of the most important emerging rumen fluke affecting livestock worldwide and the scenario is worst in tropical and sub-tropical regions. Different species of rumen fluke or paramphistomum dominate in different countries. For example, Calicophoron calicophorum is the most common species in Australia whilst Paramphistomum cervi is described as the most common species in countries as far apart as Pakistan and Mexico. In the Mediterranean and temperate regions of Algeria and Europe, Calicophoron daubneyi predominates and it has recently also been recognized as the main rumen fluke in the British Isles. Sharp increases in the prevalence of rumen fluke infections have been recorded across Western European countries. The species Calicophoron daubneyi has been identified as the primary rumen fluke parasite infecting cattle, sheep and goats in Europe. In our country Ethiopia also paramphistomum has been reported from different parts of the country. The rumen fluke life cycle requires two hosts; featuring snail intermediate host and the mammalian host usually, ruminants are the definitive host. The infection of the definitive host is initiated by the ingestion of encysted metacercariae attached to vegetation or floating in the water. Diagnosis of rumen fluke is based on the clinical sign usually involving young animals in the herd history of grazing land around the snail habitat.
  • Phylogenomic Analyses Support the Monophyly of Excavata and Resolve Relationships Among Eukaryotic ‘‘Supergroups’’

    Phylogenomic Analyses Support the Monophyly of Excavata and Resolve Relationships Among Eukaryotic ‘‘Supergroups’’

    Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic ‘‘supergroups’’ Vladimir Hampla,b,c, Laura Huga, Jessica W. Leigha, Joel B. Dacksd,e, B. Franz Langf, Alastair G. B. Simpsonb, and Andrew J. Rogera,1 aDepartment of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada B3H 1X5; bDepartment of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1; cDepartment of Parasitology, Faculty of Science, Charles University, 128 44 Prague, Czech Republic; dDepartment of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom; eDepartment of Cell Biology, University of Alberta, Edmonton, AB, Canada T6G 2H7; and fDepartement de Biochimie, Universite´de Montre´al, Montre´al, QC, Canada H3T 1J4 Edited by Jeffrey D. Palmer, Indiana University, Bloomington, IN, and approved January 22, 2009 (received for review August 12, 2008) Nearly all of eukaryotic diversity has been classified into 6 strong support for an incorrect phylogeny (16, 19, 24). Some recent suprakingdom-level groups (supergroups) based on molecular and analyses employ objective data filtering approaches that isolate and morphological/cell-biological evidence; these are Opisthokonta, remove the sites or taxa that contribute most to these systematic Amoebozoa, Archaeplastida, Rhizaria, Chromalveolata, and Exca- errors (19, 24). vata. However, molecular phylogeny has not provided clear evi- The prevailing model of eukaryotic phylogeny posits 6 major dence that either Chromalveolata or Excavata is monophyletic, nor supergroups (25–28): Opisthokonta, Amoebozoa, Archaeplastida, has it resolved the relationships among the supergroups. To Rhizaria, Chromalveolata, and Excavata. With some caveats, solid establish the affinities of Excavata, which contains parasites of molecular phylogenetic evidence supports the monophyly of each of global importance and organisms regarded previously as primitive Rhizaria, Archaeplastida, Opisthokonta, and Amoebozoa (16, 18, eukaryotes, we conducted a phylogenomic analysis of a dataset of 29–34).