MAPPE PARASSITOLOGICHE MAPPE Alghero 26-29 Giugno 2012

Total Page:16

File Type:pdf, Size:1020Kb

MAPPE PARASSITOLOGICHE MAPPE Alghero 26-29 Giugno 2012 18 SOIPA Società Italiana di Parassitologia Comune di Alghero SOCIETÀ ITALIANA DI PARASSITOLOGIA XXVII Congresso Nazionale MAPPE PARASSITOLOGICHE MAPPE Alghero 26-29 giugno 2012 Dipartimento di Medicina Veterinaria In copertina: Protoscolice di Echinococcus granulosus Università degli Studi di Sassari (Foto G. Garippa) MAPPE PARASSITOLOGICHE MAPPE MAPPE PARASSITOLOGICHE 18 Mappe Parassitologiche CONTENTS Series Editor Lettura Magistrale Giuseppe Cringoli Relazioni ai Simposi e Tavola Rotonda Copyrigth© 2012 by Giuseppe Cringoli Comunicazioni Scientifiche del XXVII Congresso Nazionale della Società Italiana di Parassitologia Registered office Veterinary Parasitology and Parasitic Diseases Alghero, 26-29 giugno 2012 Department of Pathology and Animal Health GIOVANNI GARIPPA - Nota editoriale . 3 Faculty of Veterinary Medicine University of Naples Federico II Via della Veterinaria, 1 Lettura Magistrale 80137 Naples - Italy EUGENIA TOGNOTTI - Americani, comunisti e zanzare . 7 Tel +39 081 2536283 e-mail: [email protected] website: www.parassitologia.unina.it Simposio 1 Epidemiologia della trichinellosi nell’uomo CREMOPAR e negli animali in italia e in europa Centro Regionale per il Monitoraggio delle Parassitosi Località Borgo Cioffi - Eboli (Sa) BRUSCHI F. - We can still learn something from trichinellosis: from a public health problem to a model for studying im- Tel./Fax +39 0828 347149 mune mediated diseases . 13 e-mail: [email protected] POZIO E. - Epidemiology of Trichinella infections in humans All rights reserved – printed in Italy and animals of Italy and Europe . 15 No part of this publication may be reproduced in any form or by any REINA D. - Epidemiological situation of trichinellosis in means, electronically, mechanically, by photocopying, recording or Spain . 16 otherwise, without the prior permission of the copyright owner. MARINCULIĆ A. - Trichinellosis is still the most important zoo- Printed and bound by Litografia Vigilante srl notic disease in Southeastern and Eastern Europe . 17 Via Nuova Poggioreale, 151 D - 80143 Naples - Italy Tel/Fax +39 081 5846611 - e-mail: [email protected] BANDINO E. - Updating on trichinellosis in Sardinia . 18 (continued) II Contents Mappe parassitologiche 18 RUOCCO L., FALCONE M.G., PRIMAVERA A., FERRI G. - Animal Simposio 4 Trichinellosis: Italian application of Reg. EC 2075/2005 As- Protozoi di interesse medico sessment and perspectives . 21 GOLDSCHMIDT P., DEGORGE S., DI CAVE D., CHAUMEIL C. - New test for the detection and molecular characterization of Simposio 2 Acanthamoeba . 47 Aggiornamenti sulla leishmaniosi e novità per il suo controllo HENRIQUEZ F.L., ROBERTS C.W. - Identification of novel inhi- bitors of Acanthamoeba species . 48 MIRÓ G. - Update on epidemiology and treatment of canine leishmaniosis . 25 MATTANA A., PINNA A., ERRE G., FIORI P.L., CAPPUCCINELLI P. - Biological characterization of free-living amoebae isolated BIANCIARDI P. - Miltefosine: a new and more modern treat- from cases of amoebic keratitis in Sassari . 49 ment for Canine Leishmaniasis . 27 CANO A., HENRIQUEZ F.L., MATTANA A., JAMES A., ROBERTS MCGAHIE D. - New developments in prevention – what do I C.W. - Acanthamoeba castellanii stimulates the produc- need to know about the new vaccine? . 32 tion of IL-6, IL-12 and TNF-alpha by murine macropha- ges . 50 OLIVA G., NIETO J., FOGLIA MANZILLO V., CAPPIELLO S., FIO- RENTINO E., DI MUCCIO T., SCALONE A., MORENO J., CHI- MELONI D., DI CAVE D., FIORI P.L., VISCOGLIOSI E. - Blasto- CHARRO C., BUTAUD T., GUEGAND L., MARTIN V., CUISINIER cystis sp.: a world yet to be discovered . 51 A.M., GUEGUEN S., CAÑAVATE C., GRADONI L. - Evidence for protection against active infection and disease progression in FIORI P.L., RAPPELLI P., DESSÌ D., DIAZ N., COCCO A.R., CUC- naïve dogs vaccinated with LiESP/QA-21 (CaniLeish®) ex- CURU M.A., MARGARITA V., SANCIU G., SANTONA S., HIRT R.P. posed to two consecutive Leishmania infantum transmission - Trichomonas vaginalis pathobiology . 55 seasons . 34 DEL CHIERICO F., CONCATO C., ROSSI DE GASPERIS M., RANNO S., DI PIETRANTONIO L., DI CRISTANZIANO V., BERRILLI F., DI Simposio 3 CAVE D., MURACA M., CALLEA F., PUTIGNANI L. - Genotyping La dicroceliosi negli animali da reddito: of Cryptosporidium spp. isolates from different Tanzania diagnosi, terapia e strategie di controllo areas . 58 PIERGILI FIORETTI D. - Dicrocoelium dendriticum: epidemio- CACCIÒ S.M., SANNELLA A.R., MANUALI E., TOSINI F., SENSI logical characteristics of a parasite of ruminants . 37 M., CROTTI D., POZIO E. - Pigs as natural hosts of Dienta- moeba fragilis genotypes found in humans . 59 JACQUIET P. - Dicrocoeliosis in ruminants: physiopathological aspects . 40 Gabrielli S., Girelli G., Vaia F., Santonicola M., Fakeri A., Cancrini G. - Trypanosoma cruzi in blood donors: a study started at the Umberto 1° teaching Policlinic in Rome . 60 CRINGOLI G. - Copromicroscopic diagnosis of dicrocoeliosis: what’s new? . 41 ROMI R., BOCCOLINI D., MENEGON M., GNESIVO C., PERELLI F., VALENTINI I.R., FABIANO F., PAGNOTTA F., SEVERINI F., DI LUCA M., TOMA L., SEVERINI C., CESARIO F., GRADONI L., REZZA G. ANTENUCCI P. - Control and treatment of Dicrocoelium den- - Sporadic cases of cryptic malaria (2009-2011) and the risk driticum in small ruminants . 44 of local vector-borne transmission in Italy . 61 (continued) Mappe parassitologiche 18 Contents III AVELLINO P., MAGLIACCA C., LOMBARDO F., BOVE M., BOR- VALHO L., MENDOZA DE GIVES P., SÁNCHEZ-ANDRADE R., PAZ- RUSO L., MODIANO D. - Development of a new molecular ap- SILVA A. - Control of strongyles in captive equines by using the proach for the diagnosis of Trichomonas vaginalis in its nematophagous fungus Duddingtonia flagrans . 75 natural human host . 62 BOSCO A., RINALDI L., MORGAN E.R., COLES G.C., CRINGOLI CACCIÒ S.M., SANNELLA A.R., MARIANO V., VALENTINI S., G. - The maintenance of anthelmintic efficacy in sheep and CARDETI C., BERTI F., TOSINI F., TONANZI D., POZIO E. - goats in southern Italy . 76 Zoonotic transmission of Cryptosporidium parvum in Italy caused by an uncommon genotype . 63 CASTAGNA G., SCONZA S., SEGHETTI M., TRAVERSA D., FRAN- GIPANE DI REGALBONO A., MARCER F., BERALDO P. - Efficacy DI CAVE D. - Pneumocystis jirovecii: genotypes and molecular of fenbendazole and moxidectin in horse stables with pre- analysis at mtLSU rRNA and DHPS loci . 64 vious history of resistance to benzimidazoles in cyathostomin populations . 77 DI FILIPPO E., TALIANI G., GABRIELLI S., TEMPERONI C., CAN- CRINI G. - Reactivation of Toxoplasma gondii in HIV-infected FALSONE L., NAPOLI E., GAGLIO G., BRIANTI E., GIANNETTO population from rural area of Zimbabwe . 65 S. - Milbemax® (milbemyicin oxime/praziquantel) flavored tablets in cats naturally infected by helminths: a clinical GABRIELLI S., MILADINOVIĆ TASIĆ N., OTAŠEVIĆ, MARJANOVIĆ trial. 78 G., CANCRINI G. - Visceral leishmaniosis/HIV co-infection: first case detected in Nis (southeastern Serbia), and diagno- FRANGIPANE DI REGALBONO A., MARCER F., SIGNORINI M., stic observations on further five Italian cases . 66 MIOTTO L., SORIOLO A., SOLARI BASANO F., TONDELLO L., DA- NESI P., PIETROBELLI M. - Effectiveness of Decoquinate on control of subclinical coccidiosis in naturally infected beef Tavola Rotonda cattle: preliminary results . 79 L’echinococcosi cistica in sardegna: quali possibilità di controllo? GRANDI G., KRAMER L.H., CALZOLARI A., QUARANTELLI A., RIGHI F. - Influence of a nutritional addition of Oregano es- LIGHTOWLERS M. - Evaluation of new control options for cy- sential oil (OEO) on Eimeria spp. infection prevalence and stic echinococcosis . 69 oocyst shedding dynamics in dairy cattle calves. 80 CRAIG P.S. - Historical and current perspectives for CE con- LIA R.P., DANTAS-TORRES F., MOTTA B., SCHNYDER M., SOLARI trol . 70 BASANO F., SCHIESSL B., MALLIA E., OTRANTO D. - Efficacy of milbemycin oxime/praziquantel tablets (Milbemax®- Novartis KERN P. - Human cystic echinococcosis: WHO-IWGE ultrasound Animal Health) against Thelazia callipaeda in naturally in- classification of cysts allows tailored clinical management . 71 fested dogs . 81 MESLIN F.X., MAGNINO S. - Neglected Zoonotic Diseases MANFREDI M.T., FRAQUELLI C., PICCOLINO BONIFORTI P., RIZZI (NZDs) with special focus on cystic echinococcosis . 72 R., ZANZANI S. - Effects of condensed tannin on natural coc- cidian infection in goat kids. 82 Sessione 1 MARANGI M., MORELLI V., PATI S., CAMARDA A., CAFIERO Terapia e farmacoresistenza M.A., GIANGASPERO A. - Acaricide residues in laying hens na- turally infested by red mite Dermanyssus gallinae . 83 ARIAS M.S., CAZAPAL-MONTEIRO C., VALDERRÁBANO E., MIGUÉ- LEZ S., ROIS J.L., LÓPEZ-ARELLANO M.E., MADEIRA DE CAR- PIÑEIRO P., SANCHÍS J., MIGUÉLEZ S., RODRÍGUEZ M.I., SUÁREZ (continued) IV Contents Mappe parassitologiche 18 J.L., CAZAPAL-MONTEIRO C., PAZ-SILVA A., ARIAS M.S., SÁN- T., GRAMICCIA M. - Sand fly-borne diseases: sand fly seasonal CHEZ-ANDRADE R. - Control of trematode infection can be im- dynamics and search for Leishmania and Phleboviruses in proved by means of soil fungi . 85 two different areas of Rome province, Italy . 95 POLLICINO P., RAMBOZZI L., MOLINAR MIN A.R., ROSSI L. - Ef- BONGIORNO G., BIANCHI R., PAPARCONE R., FOGLIA MANZILLO ficacy and safety of topical eprinomectin to control Myocop- V., OLIVA G., BARBATO A., GRADONI L. - Antifeeding activity tes musculinus infestation in mice . 86 of Azadirachta indica (Neem) extract against sand fly bites on dogs . 96 SANNA G., VARCASIA A., CARBONI S., PIPIA A.P., MANCA R., GENCHI C., SCALA A. -
Recommended publications
  • Pseudosuccinea Columella: Age Resistance to Calicophoron Daubneyi Infection in Two Snail Populations
    Parasite 2015, 22,6 Ó Y. Dar et al., published by EDP Sciences, 2015 DOI: 10.1051/parasite/2015003 Available online at: www.parasite-journal.org RESEARCH ARTICLE OPEN ACCESS Pseudosuccinea columella: age resistance to Calicophoron daubneyi infection in two snail populations Yasser Dar1,2, Daniel Rondelaud2, Philippe Vignoles2, and Gilles Dreyfuss2,* 1 Department of Zoology, Faculty of Science, University of Tanta, Tanta, Egypt 2 INSERM 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France Received 26 November 2014, Accepted 21 January 2015, Published online 10 February 2015 Abstract – Individual infections of Egyptian and French Pseudosuccinea columella with five miracidia of Calicoph- oron daubneyi were carried out to determine whether this lymnaeid was capable of sustaining larval development of this parasite. On day 42 post-exposure (at 23 °C), infected snails were only noted in groups of individuals measuring 1 or 2 mm in height at miracidial exposure. Snail survival in the 2-mm groups was significantly higher than that noted in the 1-mm snails, whatever the geographic origin of snail population. In contrast, prevalence of C. daubneyi infec- tion was significantly greater in the 1-mm groups (15–20% versus 3.4–4.0% in the 2-mm snails). Low values were noted for the mean shell growth of infected snails at their death (3.1–4.0 mm) and the mean number of cercariae (<9 in the 1-mm groups, <19 in the 2-mm snails). No significant differences between snail populations and snails groups were noted for these last two parameters. Most infected snails died after a single cercarial shedding wave.
    [Show full text]
  • Multigene Eukaryote Phylogeny Reveals the Likely Protozoan Ancestors of Opis- Thokonts (Animals, Fungi, Choanozoans) and Amoebozoa
    Accepted Manuscript Multigene eukaryote phylogeny reveals the likely protozoan ancestors of opis- thokonts (animals, fungi, choanozoans) and Amoebozoa Thomas Cavalier-Smith, Ema E. Chao, Elizabeth A. Snell, Cédric Berney, Anna Maria Fiore-Donno, Rhodri Lewis PII: S1055-7903(14)00279-6 DOI: http://dx.doi.org/10.1016/j.ympev.2014.08.012 Reference: YMPEV 4996 To appear in: Molecular Phylogenetics and Evolution Received Date: 24 January 2014 Revised Date: 2 August 2014 Accepted Date: 11 August 2014 Please cite this article as: Cavalier-Smith, T., Chao, E.E., Snell, E.A., Berney, C., Fiore-Donno, A.M., Lewis, R., Multigene eukaryote phylogeny reveals the likely protozoan ancestors of opisthokonts (animals, fungi, choanozoans) and Amoebozoa, Molecular Phylogenetics and Evolution (2014), doi: http://dx.doi.org/10.1016/ j.ympev.2014.08.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 1 Multigene eukaryote phylogeny reveals the likely protozoan ancestors of opisthokonts 2 (animals, fungi, choanozoans) and Amoebozoa 3 4 Thomas Cavalier-Smith1, Ema E. Chao1, Elizabeth A. Snell1, Cédric Berney1,2, Anna Maria 5 Fiore-Donno1,3, and Rhodri Lewis1 6 7 1Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
    [Show full text]
  • Distribution of Fasciola Spp, Dicrocoelium
    SUSTAINABLE DEVELOPMENT, CULTURE, TRADITIONS Journal Special Volume in Honor of Professor George I. Theodoropoulos DISTRIBUTION OF FASCIOLA SPP, DICROCOELIUM DENDRITICUM AND CALICOPHORON DAUBNEYI IN SMALL RUMINANTS IN THE MEDITERRANEAN BASIN: A SYSTEMATIC REVIEW DOI: 10.26341/issn.2241-4002-2019-sv-5 Vaia Kantzoura Department of Anatomy and Physiology of Farm Animals, Faculty of Animal Science and Aquaculture, Agricultural University of Athens, Greece. Veterinary Research Institute, HAO-Demeter, NAGREF Campus, Thessaloniki, Greece. [email protected] Marc K. Kouam Department of Animal Science, Faculty of Agronomy and Agricultural Sciences, Cameroon Center for Research on Filariases and other Tropical Diseases (CRFilMT), Yaoundé, Cameroon Abstract The most common flukes found in sheep in Europe, Asia and Africa are the liver flukes Fasciola spp., Dicrocoelium dentriticum and the rumen fluke Calicophoron daubneyi. The prevalence of F. hepatica in Europe varies among countries (from 47.3 % in Greece, 57.4 % in Spain, and 95% in Italy). Several studies have been conducted in Africa and Asia as concern the prevalence of Fasciola in sheep but limited studies have been done in goats. The prevalence of D. dentriticum in sheep has been reported to be 6.7-86.2% in Italy and to range from 0.2%- to 70% in Greece. The prevalence of D. dentriticum in sheep was found to be 5% in Egypt and 3,85 to 23,55% in Turkey. Only three surveys have been conducted to measure the prevalence of D. dentriticum in goats in the Mediterranean basin indicating a variation from 0.9% to 42.42 %. C. daubneyi is the dominant rumen fluke species in Europe with significant clinical importance in ruminants.
    [Show full text]
  • Author's Manuscript (764.7Kb)
    1 BROADLY SAMPLED TREE OF EUKARYOTIC LIFE Broadly Sampled Multigene Analyses Yield a Well-resolved Eukaryotic Tree of Life Laura Wegener Parfrey1†, Jessica Grant2†, Yonas I. Tekle2,6, Erica Lasek-Nesselquist3,4, Hilary G. Morrison3, Mitchell L. Sogin3, David J. Patterson5, Laura A. Katz1,2,* 1Program in Organismic and Evolutionary Biology, University of Massachusetts, 611 North Pleasant Street, Amherst, Massachusetts 01003, USA 2Department of Biological Sciences, Smith College, 44 College Lane, Northampton, Massachusetts 01063, USA 3Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA 4Department of Ecology and Evolutionary Biology, Brown University, 80 Waterman Street, Providence, Rhode Island 02912, USA 5Biodiversity Informatics Group, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA 6Current address: Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA †These authors contributed equally *Corresponding author: L.A.K - [email protected] Phone: 413-585-3825, Fax: 413-585-3786 Keywords: Microbial eukaryotes, supergroups, taxon sampling, Rhizaria, systematic error, Excavata 2 An accurate reconstruction of the eukaryotic tree of life is essential to identify the innovations underlying the diversity of microbial and macroscopic (e.g. plants and animals) eukaryotes. Previous work has divided eukaryotic diversity into a small number of high-level ‘supergroups’, many of which receive strong support in phylogenomic analyses. However, the abundance of data in phylogenomic analyses can lead to highly supported but incorrect relationships due to systematic phylogenetic error. Further, the paucity of major eukaryotic lineages (19 or fewer) included in these genomic studies may exaggerate systematic error and reduces power to evaluate hypotheses.
    [Show full text]
  • Chronic Wasting Due to Liver and Rumen Flukes in Sheep
    animals Review Chronic Wasting Due to Liver and Rumen Flukes in Sheep Alexandra Kahl 1,*, Georg von Samson-Himmelstjerna 1, Jürgen Krücken 1 and Martin Ganter 2 1 Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany; [email protected] (G.v.S.-H.); [email protected] (J.K.) 2 Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany; [email protected] * Correspondence: [email protected] Simple Summary: Chronic wasting in sheep is often related to parasitic infections, especially to infections with several species of trematodes. Trematodes, or “flukes”, are endoparasites, which infect different organs of their hosts (often sheep, goats and cattle, but other grazing animals as well as carnivores and birds are also at risk of infection). The body of an adult fluke has two suckers for adhesion to the host’s internal organ surface and for feeding purposes. Flukes cause harm to the animals by subsisting on host body tissues or fluids such as blood, and by initiating mechanical damage that leads to impaired vital organ functions. The development of these parasites is dependent on the occurrence of intermediate hosts during the life cycle of the fluke species. These intermediate hosts are often invertebrate species such as various snails and ants. This manuscript provides an insight into the distribution, morphology, life cycle, pathology and clinical symptoms caused by infections of liver and rumen flukes in sheep.
    [Show full text]
  • Third Target of Rapamycin Complex Negatively Regulates Development of Quiescence in Trypanosoma Brucei
    Third target of rapamycin complex negatively regulates development of quiescence in Trypanosoma brucei Antonio Barquillaa, Manuel Saldiviaa, Rosario Diaza, Jean-Mathieu Barta,b, Isabel Vidala, Enrique Calvoc, Michael N. Halld, and Miguel Navarroa,1 aInstituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas (CSIC), 18100 Granada, Spain; bCentro Nacional de Medicina Tropical, The Institute of Health Carlos III, 28029 Madrid, Spain; cCentro Nacional de Investigaciones Cardiovasculares, 28029 Madrid, Spain; and dBiozentrum, University of Basel, CH4056 Basel, Switzerland Edited by Alberto Carlos Frasch, Universidad de San Martin and National Research Council, San Martin, Argentina, and approved July 27, 2012 (receivedfor review June 21, 2012) African trypanosomes are protozoan parasites transmitted by that TbTOR4 assembles into a structurally and functionally a tsetse fly vector to a mammalian host. The life cycle includes unique TOR complex (TbTORC4) that plays a crucial role in the highly proliferative forms and quiescent forms, the latter being T. brucei life cycle. adapted to host transmission. The signaling pathways controlling TbTOR4 contains characteristic TOR kinase domains, in- the developmental switch between the two forms remain un- cluding HEAT, FAT, and FATC domains, but lacks a rapamy- known. Trypanosoma brucei contains two target of rapamycin cin-binding site (RBS). The RBSs in TbTOR1 and TbTOR3 also (TOR) kinases, TbTOR1 and TbTOR2, and two TOR complexes, are poorly conserved and do not interact with FKBP2-rapamycin TbTORC1 and TbTORC2. Surprisingly, two additional TOR kinases (5). Multiple-alignment analysis of TbTOR4 with other members are encoded in the T. brucei genome. We report that TbTOR4 asso- of the PI3K-related kinase (PIKK) superfamily indicates that ciates with an Armadillo domain-containing protein (TbArmtor), TbTOR4 clusters with the TOR family (Fig.
    [Show full text]
  • Diplomarbeit
    DIPLOMARBEIT Titel der Diplomarbeit „Microscopic and molecular analyses on digenean trematodes in red deer (Cervus elaphus)“ Verfasserin Kerstin Liesinger angestrebter akademischer Grad Magistra der Naturwissenschaften (Mag.rer.nat.) Wien, 2011 Studienkennzahl lt. Studienblatt: A 442 Studienrichtung lt. Studienblatt: Diplomstudium Anthropologie Betreuerin / Betreuer: Univ.-Doz. Mag. Dr. Julia Walochnik Contents 1 ABBREVIATIONS ......................................................................................................................... 7 2 INTRODUCTION ........................................................................................................................... 9 2.1 History ..................................................................................................................................... 9 2.1.1 History of helminths ........................................................................................................ 9 2.1.2 History of trematodes .................................................................................................... 11 2.1.2.1 Fasciolidae ................................................................................................................. 12 2.1.2.2 Paramphistomidae ..................................................................................................... 13 2.1.2.3 Dicrocoeliidae ........................................................................................................... 14 2.1.3 Nomenclature ...............................................................................................................
    [Show full text]
  • A Free-Living Protist That Lacks Canonical Eukaryotic DNA Replication and Segregation Systems
    bioRxiv preprint doi: https://doi.org/10.1101/2021.03.14.435266; this version posted March 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 A free-living protist that lacks canonical eukaryotic DNA replication and segregation systems 2 Dayana E. Salas-Leiva1, Eelco C. Tromer2,3, Bruce A. Curtis1, Jon Jerlström-Hultqvist1, Martin 3 Kolisko4, Zhenzhen Yi5, Joan S. Salas-Leiva6, Lucie Gallot-Lavallée1, Geert J. P. L. Kops3, John M. 4 Archibald1, Alastair G. B. Simpson7 and Andrew J. Roger1* 5 1Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB), Department of 6 Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2 2 7 Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom 8 3Oncode Institute, Hubrecht Institute – KNAW (Royal Netherlands Academy of Arts and Sciences) 9 and University Medical Centre Utrecht, Utrecht, The Netherlands 10 4Institute of Parasitology Biology Centre, Czech Acad. Sci, České Budějovice, Czech Republic 11 5Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life Science, 12 South China Normal University, Guangzhou 510631, China 13 6CONACyT-Centro de Investigación en Materiales Avanzados, Departamento de medio ambiente y 14 energía, Miguel de Cervantes 120, Complejo Industrial Chihuahua, 31136 Chihuahua, Chih., México 15 7Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB), Department of 16 Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2 17 *corresponding author: [email protected] 18 D.E.S-L ORCID iD: 0000-0003-2356-3351 19 E.C.T.
    [Show full text]
  • HE 1721-2015 Varcassia-Final.Indd
    ©2016 Institute of Parasitology, SAS, Košice DOI 10.1515/helmin-2015-0069 HELMINTHOLOGIA, 53, 1: 87 – 93, 2016 Research Note Calicophoron daubneyi in sheep and cattle of Sardinia, Italy G. SANNA1, A. VARCASIA1*, S. SERRA1, F. SALIS2, R. SANABRIA3, A. P. PIPIA1, F. DORE1, A. SCALA1,4 1Laboratory of Parasitology, Veterinary Teaching Hospital, Veterinary Department, University of Sassari, Italy, *E-mail: [email protected]; 2Veterinary Practitioner, Martini Zootecnica, Italy; 3Veterinary Faculty, National University of La Plata, Buenos Aires, Argentine. National Scientifi c and Technical Research Council (CONICET), Argentine; 4Inter-University Center for Research in Parasitology (CIRPAR), Via della Veterinaria 1 – 80137, Naples, Italy Article info Summary Received April 22, 2015 This study aimed to investigate the prevalence of paramphistomosis and confi rm the species identity Accepted July 13, 2015 of rumen fl ukes from sheep and cattle of Sardinia (Italy), by molecular methods. From 2011 to 2014, 381 sheep and 59 cattle farms were selected and individual faecal samples were run on 15 sheep and 5 cattle for each farm, respectively. The prevalence at the slaughterhouse was calculated by examination of 356 sheep and 505 cattle. 13adult fl ukes collected from sheep and cattle and 5 belonging to the historical collection of Laboratory of Parasitology at the Department of Veterinary Medicine of Sassari, previously classifi ed as Paramphistomum spp., were used for PCR amplifi ca- tion and sequencing of the ITS2+ rDNA. Previously classifi ed Paramphistomum leydeni from South America were used as controls. The EPG prevalence was 13.9 % and 55.9 % for sheep and cattle farms respectively. At slaughter- houses, paramphistomes were found in 2 % of the sheep and 10.9 % of the examined cows.
    [Show full text]
  • Leishmania LABCG1 and LABCG2 Transporters
    Manzano et al. Parasites & Vectors (2017) 10:267 DOI 10.1186/s13071-017-2198-1 RESEARCH Open Access Leishmania LABCG1 and LABCG2 transporters are involved in virulence and oxidative stress: functional linkage with autophagy José Ignacio Manzano1, Ana Perea1, David León-Guerrero1, Jenny Campos-Salinas1, Lucia Piacenza2, Santiago Castanys1*† and Francisco Gamarro1*† Abstract Background: The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that Leishmania LABCG2 transporter plays a role in the exposure of phosphatidylserine (PS), in virulence and in resistance to antimonials. However, the involvement of this transporter in other key biological processes has not been studied. Methods: To better understand the biological function of LABCG2 and its nearly identical tandem-repeated transporter LABCG1, we have generated Leishmania major null mutant parasites for both genes (ΔLABCG1-2). NBD-PS uptake, infectivity, metacyclogenesis, autophagy and thiols were measured. Results: Leishmania major ΔLABCG1-2 parasites present a reduction in NBD-PS uptake, infectivity and virulence. In addition, we have shown that ΔLABCG1-2 parasites in stationary phase growth underwent less metacyclogenesis and presented differences in the plasma membrane’s lipophosphoglycan composition. Considering that autophagy is an important process in terms of parasite virulence and cell differentiation, we have shown an autophagy defect in ΔLABCG1-2 parasites, detected by monitoring expression of the autophagosome marker RFP-ATG8. This defect correlates with increased levels of reactive oxygen species and higher non-protein thiol content in ΔLABCG1-2 parasites. HPLC analysis revealed that trypanothione and glutathione were the main molecules accumulated in these ΔLABCG1-2 parasites.
    [Show full text]
  • Review on Paramphistomosis
    Advances in Biological Research 14 (4): 184-192, 2020 ISSN 1992-0067 © IDOSI Publications, 2020 DOI: 10.5829/idosi.abr.2020.184.192 Review on Paramphistomosis 12Adane Seifu Hotessa and Demelash Kalo Kanko 1Hawassa University, Revenue Generating PLC Farm, P.O. Box: 05, Hawassa, Ethiopia 2Gerese Woreda Livestock and Fishery Resource Office, Gamo Zone, SNNPR, Ethiopia Abstract: Paramphistomum is considered to be one of the most important emerging rumen fluke affecting livestock worldwide and the scenario is worst in tropical and sub-tropical regions. Different species of rumen fluke or paramphistomum dominate in different countries. For example, Calicophoron calicophorum is the most common species in Australia whilst Paramphistomum cervi is described as the most common species in countries as far apart as Pakistan and Mexico. In the Mediterranean and temperate regions of Algeria and Europe, Calicophoron daubneyi predominates and it has recently also been recognized as the main rumen fluke in the British Isles. Sharp increases in the prevalence of rumen fluke infections have been recorded across Western European countries. The species Calicophoron daubneyi has been identified as the primary rumen fluke parasite infecting cattle, sheep and goats in Europe. In our country Ethiopia also paramphistomum has been reported from different parts of the country. The rumen fluke life cycle requires two hosts; featuring snail intermediate host and the mammalian host usually, ruminants are the definitive host. The infection of the definitive host is initiated by the ingestion of encysted metacercariae attached to vegetation or floating in the water. Diagnosis of rumen fluke is based on the clinical sign usually involving young animals in the herd history of grazing land around the snail habitat.
    [Show full text]
  • Phylogenomic Analyses Support the Monophyly of Excavata and Resolve Relationships Among Eukaryotic ‘‘Supergroups’’
    Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic ‘‘supergroups’’ Vladimir Hampla,b,c, Laura Huga, Jessica W. Leigha, Joel B. Dacksd,e, B. Franz Langf, Alastair G. B. Simpsonb, and Andrew J. Rogera,1 aDepartment of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada B3H 1X5; bDepartment of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1; cDepartment of Parasitology, Faculty of Science, Charles University, 128 44 Prague, Czech Republic; dDepartment of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom; eDepartment of Cell Biology, University of Alberta, Edmonton, AB, Canada T6G 2H7; and fDepartement de Biochimie, Universite´de Montre´al, Montre´al, QC, Canada H3T 1J4 Edited by Jeffrey D. Palmer, Indiana University, Bloomington, IN, and approved January 22, 2009 (received for review August 12, 2008) Nearly all of eukaryotic diversity has been classified into 6 strong support for an incorrect phylogeny (16, 19, 24). Some recent suprakingdom-level groups (supergroups) based on molecular and analyses employ objective data filtering approaches that isolate and morphological/cell-biological evidence; these are Opisthokonta, remove the sites or taxa that contribute most to these systematic Amoebozoa, Archaeplastida, Rhizaria, Chromalveolata, and Exca- errors (19, 24). vata. However, molecular phylogeny has not provided clear evi- The prevailing model of eukaryotic phylogeny posits 6 major dence that either Chromalveolata or Excavata is monophyletic, nor supergroups (25–28): Opisthokonta, Amoebozoa, Archaeplastida, has it resolved the relationships among the supergroups. To Rhizaria, Chromalveolata, and Excavata. With some caveats, solid establish the affinities of Excavata, which contains parasites of molecular phylogenetic evidence supports the monophyly of each of global importance and organisms regarded previously as primitive Rhizaria, Archaeplastida, Opisthokonta, and Amoebozoa (16, 18, eukaryotes, we conducted a phylogenomic analysis of a dataset of 29–34).
    [Show full text]