Author Manuscript Published OnlineFirst on December 4, 2017; DOI: 10.1158/1078-0432.CCR-17-2542 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. 1 Spatially resolved and quantitative analysis of VISTA/PD-1H as a novel immunotherapy target in 2 human non-small cell lung cancer 3 Franz Villarroel-Espindolaa, Xiaoqing Yub, Ila Datara, Nikita Mania, Miguel Sanmamedc, Vamsidhar 4 Velchetid, Konstantinos Syrigose, Maria Tokia, Hongyu Zhaob, Lieping Chenc, Roy S. Herbstf and Kurt A. 5 Schalpera,f 6 aDepartment of Pathology, Yale School of Medicine, New Haven, CT, 06520 USA. 7 bDepartment of Public Health, Yale School of Medicine, New Haven, CT, 06520 USA. 8 cImmunobiology, Yale School of Medicine, New Haven, CT, 06520 USA. 9 dSolid Tumor Oncology, Cleveland Clinic Foundation, Cleveland, OH, 44195 USA. 10 eOncology Unit GPP, Athens School of Medicine, Greece. 11 fMedical Oncology and Yale Cancer Center, New Haven, CT, 06520 USA. 12 Running title: Role of VISTA/PD-1H in NSCLC. 13 Keywords: NSCLC, VISTA/PD-1H, PD-1 pathway, TILs 14 Correspondence to: 15 Kurt A. Schalper, M.D./Ph.D. 16 Office Address: 333 Cedar St. FMP117, New Haven, CT 06520-8023 17 Email:
[email protected] 18 Financial support: 19 Lung Cancer Research Foundation (LCRF), Yale SPORE in Lung Cancer (P50CA196530), Department of 20 Defense-Lung Cancer Research Program Career Development Award (LC150383), Stand Up to Cancer – 21 American Cancer Society Lung Cancer Dream Team Translational Research Grant (Grant Number: SU2C- 22 AACR-DT17-15). Stand Up To Cancer is a program of the Entertainment Industry Foundation.