Curriculum Vitae s293

Curriculum Vitae Name: Shahid Bashir Office Address: Berenson-Allen Center for Noninvasive Brain Stimulation Beth Israel Deaconess Medical Center 330 Brookline Avenue, Kirstein Building, Room KS158 Boston, MA 02215 United States Office Address: Assistant Professor of KSU-Autism Research and Treatment center Department of physiology, Faculty of Medicine King Saud University, P O Box 2925, Riyadh 11461 Saudi Arabia Work Phone: 001-617-667-0383 Work Email: [email protected] [email protected] Work FAX: 617-975-5322 Place of Birth: Lahore, Pakistan

Position 1. 2011-present: Instructor of Harvard Medical Beth Israel Deaconess Medical Center school 330 Brookline Avenue, Kirstein Building, Room KS158 Boston, MA 02215 United States. 2. 2011-present: Assistant Professor of KSU-Autism Research and Treatment center Department of physiology, Faculty of Medicine King Saud University, P O Box 2925, Riyadh 11461 Saudi Arabia

Education 1998 B.Sc. Chemistry and Biology Univ. of Punjab Lahore, Pakistan 2001 M.Sc. Biology Univ. of Punjab Lahore, Pakistan 2007 Ph.D. Physiology Univ. of Fribourg Fribourg, Switzerland

Postdoctoral Training 2007 - 2008 Postdoctoral Fellow Neurophysiology Univ. of Fribourg Fribourg, Switzerland 2008 - 2010 Research Fellow Neurology Harvard University Beth Israel Deaconess Medical Center, Boston, MA

1 Other Professional Positions 2003 - 2006 Visiting Fellow CHUV Hospital, University of Lausanne, Switzerland 2006 Visiting Trainee University of Bern, Department of Medicine, Switzerland 2007 Visiting Trainee Universtiy of Zurich, Department of Neurobiology, Switzerland

Professional Societies

2004-present Swiss Society of Neuroscience Member 2004-present Federation European Neuroscience Society Member

Honors and Prizes 2000 Presidential fellowship Pakistan Higher education Fellowship for talent student 2007 Travel Grant Swiss Society of Neuroscience

Report of Funded and Unfunded Projects Past

2003-2006 Fellowship from University of Fribourg. Rehabilitation of stroke and cerebellar patients Study design, recorded and analysis data on stroke and Cerbeller patients populations (part od thesies work)

2003-2008 Novartis Spontaneous functional recovery of manual dexterity resulting from a lesion of the motor cortex treated with neurite outgrowth inhibitor Nogo-A in primates Co-investigator Recorded and analyzed neurophysiologic activity from motor cortex treated with neurite outgrowth inhibitor Nogo-A in primates

2008-2011 National Institutes of Health, National Institute for Deafness and Communication Disorders, RO1-DC05672 Transcranial Magnetic Stimulation to Improve Speech Co-Investigator Examine the potential use of repetitive transcranial magnetic stimulation as therapy for non-fluent aphasia

2 2009-2011 International Brain Research Foundation Promoting Recovery from Traumatic Brain Injury Co-Investigator To test cortico-cortical connectivity, plasticity and network dynamics using the real-time combination of transcranial magnetic stimulation and electroencephalography (EEG) as a predictor of recovery from mild TBI

2010 – 2011 CIMIT Characterization of Cortical Plasticity after Mild Traumatic Brain Injury in Humans Co-Investigator To test cortico-cortical connectivity, plasticity and network dynamics using the real-time combination of transcranial magnetic stimulation and electroencephalography (EEG)

Current Unfunded Projects

2011 - 2013 National Institutes of Health (R21 under review) Noninvasive, Physiologic Characterization of Cortical Plasticity after Mild Traumatic Brain Injury in Humans Co-Investigator To test cortico-cortical connectivity, plasticity and network dynamics using the real-time combination of transcranial magnetic stimulation and electroencephalography (EEG) in Mild Traumatic Brain Injury patients

Report of Local Teaching and Training Teaching of Students in Courses

2003 - 2007 Practical course for Second year medical University of Fribourg students (Neurophysiology), ~50 students Three hours per day for three weeks 2008 – Intensive Course in Transcranial Magnetic Harvard Medical school Stimulation. ~10 to 15 students per course. 9 hours preparation and contact time per course (three courses a year).

Local Invited Presentations

2008 Poststroke Rehabilitation, Invited Lecturer BIDMC, Neurology

3 Report of Regional, National and International Invited Teaching and Presentations Invited Presentations and Courses International

2007 “Poststroke Rehabilitation in Pakistan” Seminar, Invited Speaker University of health science, Department of Physiology, Lahore, Pakistan

Publications Peer reviewed publications in print or other media

1.Zaheer A, Akhtar T and Bashir S (1999): Echinoucus granulosus , histopathology of naturally infected sheep liver. Punjab univ.j.zool. Vol. No. 14. 105-111. Record number: PK2001001205 2.Diserens K , Herrmann F , Perret N , Chatelain S , Filipovic N , Bashir S , Ruegg D , Vuadens, P , Bogousslavsky J , Vingerhoets F (2007): The effect of repetitive arm cycling on post stroke spasticity and motor control. J Neurol Sci. 15; 253(1-2):18-24. PMID: 17241642 3.Stamoulis C, Praeg E, Bashir S, Chang BS, Pascual-Leone A (2009): Estimation of Brain State Changes Associated with Behavior, Stimulation and Epilepsy. Conf Proc IEEE Eng Med Biol Soc. 4719-22. PMID: 19964837 4.Kaeser M, Wyss A, Bashir S, Hamadjida A, Liu Y, Bloch J, Brunet JF, Saif AB and Rouiller EM (2010): Long-Term effects of Unilateral Lesion of the Primary Motor Cortex (M1) on Ipsilesional Hand Dexterity in Adult Macaque Monkeys: relationship with functional recovery of the contralesional hand. J Neurophysiol. 103(3):1630-45. PMID: 20071636 5.Bashir S, Edwards D, and Pascual-Leone A (2010): Neuro-navigation increases the effectiveness of low-frequency rTMS on primary motor cortex in healthy subjects. Brain Topogr. (ahead of press on- line publication Nov 13). PMID: 21076861 6.Oberman L, Ifert-Miller F, Najib U, Bashir S, Woollacott I, Gonzalez-Heydrich J, Picker J, Rotenberg A and Pascual-Leone A (2010): Transcranial magnetic stimulation provides means to assess cortical plasticity and excitability in humans with fragile X syndrome and autism spectrum disorder. Front. Syn. Neurosci. 2:26. (ahead of press on-line publication Nov 13) doi:10.3389/fnsyn.2010.00026 7.Stamoulis S, Oberman L, Praeg E, Bashir S, Pascual-Leone A (2011): Single Pulse TMS-Induced Modulations of Resting Brain Neurodynamics Encoded in EEG Phase. Brain Topogr. PMID: 21203817 8.Bashir S, Kaeser M, Wyss A, Hamadjida A, Liu Y, Bloch J, Brunet JF, Belhaj-Saif A, Rouiller EM. Short-term effects of unilateral lesion of the primary motor cortex (M1) on ipsilesional hand dexterity in adult macaque monkeys. Brain Struct Funct. 2011 May 20. PMID: 21597965 9.Freitas C, Perez J, Knobel M, Tormos JM, Oberman L, Eldaief M, Bashir S, Vernet M, Peña- Gómez C, Pascual-Leone A. Changes in cortical plasticity across the lifespan. Front Aging Neurosci. 2011; 3:5. PMID: 21519394

Reviews/Chapters/Editorials

4 1.Bashir S , Mizrahi I , Weaver K , Fregni F, Pascual-Leone A (2010): Assessment and modulation of neuroplasticity in rehabilitation with transcranial magnetic stimulation. Physical Medicine and Rehabilitation. 2 (12) Supplement: S253-S268. DOI: 10.1016/j.pmrj.2010.10.015) 2.Naeser MA, Martin PI, Treglia E, Ho M, Kaplan E, Bashir S, Hamilton R, Coslett HB, Pascual- Leone A (2010): Research with rTMS in the treatment of aphasia. Restor Neurol Neurosci. 28 (4):511-29. PMID: 20714075 3.Ekhtiari H and Bashir S (2010): Brain Stimulation Technology in Addiction Medicine; Main Problems Waiting for Solutions. Basic And Clinical Neuroscience. 1(4): 3-4. BCN 1389 http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-37&slc_lang=en&sid=1 4.Bashir S, Sikaroudi H, Kazemi R and Ekhtiari H (2010): Integrated technologies like noninvasive brain stimulation (NIBS) for evaluation in rehabilitation in stroke; New hopes for patients, neuroscientists, and clinicians in Iran. Basic And Clinical Neuroscience. 1(4): 6-14; BCN 1389. http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-38&slc_lang=en&sid=1 5.Najib U, Bashir S, Edwards D, Rotenberg A, Pascual-Leone A (2011): Transcranial Brain Stimulation: Clinical Applications and Future Directions. Neurosurgery Clinics of North America 6.Naeser MA, Martin P , Ho M, Treglia E, Kaplan E, Bhashir S, Pascual-Leone A (2011): Transcranial Magnetic Stimulation and Aphasia Rehabilitation. Article for Summer Supplement, on Language-based Disorders in Rehabilitation For Archives of Physical Medicine and Rehabilitation 7.Pascual-Leone A, Freitas C, Oberman L, Horvath JC, Halko M, Eldaief M, Bashir S, Vernet M, Shafi M, Westover B, Vahabzadeh-Hagh AM, Rotenberg A. Characterizing Brain Cortical Plasticity and Network Dynamics Across the Age-Span in Health and Disease with TMS-EEG and TMS- fMRI. Brain Topogr. 2011 Aug 14

Thesis

Bashir S (2007): Rehabilitation of stroke and cerebellar patients. Fribourg, Switzerland: University of Fribourg, Department physiology. http://ethesis.unifr.ch/theses/downloads.php?file=BashirS.pdf

Abstracts, Poster Presentations and Exhibits Presented at Professional Meetings

1.Bashir S and Dieter R. Estimation of the input-force relation of a muscle by superposition of contraction. The Annual Meeting of the Swiss Society for Neuroscience (2003), Fribourg, Switzerland. 2.Bashir S and Dieter R. The effect of gravity and negative pressure in the lower body on cycling. USGEB meeting (2004) Fribourg, Switzerland. 3.Bashir S, Kaeser M, Beaud ML, Freund P, Wyss A, Belhaj-Saif A, Bloch J, Debatisse D, Mir A, Pralong E, Rouiller E and Wannier T. Characterization of early somatosensory evoked potentials (SEPs) to electrical stimulation of the median nerve in adult macaque monkeys: intact and spinal lesioned animals. Society of Neuroscience (2007), 37th annual meeting, San Diego, CA 4.Bashir S, Kaeser M, Beaud ML, Freund P, Wyss A, Belhaj-Saif A, Bloch J, Debatisse D, Mir A, Pralong E, Rouiller E and Wannier T. Effects of a combination of ketamine and medetomidine hydrochloride (KM) anaesthesia on somatosensory evoked potentials (SEPs) obtained by electrical stimulation of the median nerve in macaque monkeys. The Annual Meeting of the Swiss Society for Neuroscience (2007) Bern, Switzerland. 5.Naeser MA, Martin P, Treglia E, Ho M, Baker E, Kaplan E, Bashir S, Pascual-Leone A. Improved

5 Action Naming in a Severe, Nonfluent Aphasia Patient following Transcranial Magnetic Stimulation plus Constraint-Induced Language Therapy. Academy of Aphasia 47th Annual Meeting. (2009), Boston, MA. 6.Eric S, Adjia H, Joël B, Bashir S, Belhaj-Saïf A, Rouiller E and Henri-Marcel H. Effect of Anti Nogo-A antibody treatment on cortical lesion of the motor cortex of macaque monkeys assessed on Magnetic Resonance Imagery (MRI). The Annual Meeting of the Swiss Society for Neuroscience (2009), Fribourg, Switzerland. 7.Rouiller E , Kaeser M, Wyss A, Hamadjida A, Bashir S, Liu Y, Bloch J, Brunet Jean-F and Belhaj- Saïf A. Role of the primary motor cortex (M1) in the control of fine manual dexterity of the homolateral hand as assessed in a model of unilateral lesion of M1 in macaque monkeys. The Annual Meeting of the Swiss Society for Neuroscience (2009), Fribourg, Switzerland. 8.Alonso-Alonso M, Praeg E, Huang XL, Sidhu AS, Bashir S, Pascual-Leone A. Brain responses associated with the caloric content of food with and without awareness: relevance for uncontrolled eating. Society of Neuroscience (2009), 39th annual meeting, Oct. 17-21 in Chicago. 9.Schmidlin E, Hamadjida A, Baechler J, Bashir S, Belhaj-Saïf A, Rouiller E and Henri-Marcel H. Effect of anti Nogo-A antibody treatment on cortical lesion of the primary motor cortex (M1) of macaque monkeys assessed with Magnetic Resonance Imaging (MRI) correlated with manual dexterity recovery. Federation of European Neuroscience (2010) Amsterdam, Netherlands. 10.Pascual-Leone A, Oberman L, Freitas C, Stamoulis C, & Bashir S. Assessing cortical plasticity in health and disease across the human lifespan. Society of Neuroscience (2010), 40th annual meeting, San Diego, CA. 11.Luna Serrano M, Bashir S , Cáceres Taladriz C, Tormos Muñoz JM, Gómez Aguilera EJ, Pascual- Leone A. Aportaciones del guiado estereotáctico sin marco a la Estimulación Magnética Transcraneal con finalidades terapéuticas.CASEIB (2010) XXVIII Congreso Anual de la Sociedad Española de Ingeniería Biomédica, Madrid, Spain. 11.Marine VERNET, Shahid BASHIR, Edwin ROBERTSON, Alvaro PASCUAL-LEONE. Motor cortical and distributed network modulation during visuo-motor learning: a TMS-EEG study. Spain. Vision Science Society Meeting, May 2011, United State of America 11.Marine VERNET, Shahid BASHIR, Jennifer M. PEREZ, Umer NAJIB, Alvaro PASCUAL- LEONE. Motor plasticity induced by theta burst stimulation: correlated measures of MEPs and EEG. Organization for Human Brain Mapping, June 2011. Canada 11.Bashir S, Marine V, Woo-Kyoung Y, Ilan M, Hugo T and Pascual-Leone A. Noninvasive, Physiologic Characterization of Cortical Plasticity After Mild Traumatic Brain Injury in Humans. Organization for Human Brain Mapping, June 2011. Canada

Submitted manscripts under review: 1.Bashir S, Perez J, and Pascual-Leone A. Differentiation of motor cortical representation of hand muscles by navigated mapping of optimal TMS current directions in healthy subjects. Human Brain Mapping (under review) 3.Stephan M, Krattinger S, Pasquier J, Bashir S, Fournier T, Ruegg D, Diserens K. Three-dimensional motion analysis of the effect of climbing on cerebellar ataxia. J. Neurophysiology (under review) 4.Bashir S, Stephan M, Perret N, Paquier J, Fournier T, Vingerhoets F, Ruegg D, Diserens K. Climbing as therapy for the patients with cerebellar disorder part 1(pilot study). The Cerebellum (under review).

Studies completed manuscripts in preparation: 1.Bashir S, Perez J, Caipa A, Alonso-Alonso M and Pascual-Leone A. Differential effects of motor 6 cortex excitability and plasticity in young and old individuals: a Transcranial Magnetic Stimulation (TMS) study. 2.Bashir S, Vernet M, Perez J, Caipa A, Alonso-Alonso M and Pascual-Leone A. Intracortical excitatory and inhibitory phenomena to navigated paired transcranial magnetic stimulation in healthy human subjects. 3.Bashir S, Vernet M, Perez J and Pascual-Leone A. Plasticity measurement (EEG and EMG) after cTBS in normal subjects. 4.Bashir S, Alonso-Alonso M, Silvanto J and Pascual-Leone A. TMS and neuromoodulation assessment for racism. 5.Bashir S, Najib N, Perez J, Vernet M and Pascual-Leone A. Improvement of neurophysiology and motor function by navigated low-frequency repetitive transcranial magnetic stimulation over the contralesional motor cortex in stroke. 6.Praeg E, Bashir S, & Pascual-Leone A. Time specific activation increase in frontal and parietal areas after classical conditioning using TMS as unconditioned stimulus. 7.Praeg E, Bashir S, & Pascual-Leone A. Neuronal effects of TMS due to task-related brain states.

Narrative Report Research My graduate work at the University of Fribourg and Rehabilitation Center, CHUV Hospital, University of Lausanne, Switzerland engendered my interest in the field of neurological rehabilitation. My graduate work helped me develop clinical skills to work with neurological patients as well as technical skills related to programming and analysis of data. In my graduate work, I developed a keen interest in approaches to alleviate movement dysfunction following stroke and cerebellar disorders. The underlying neural mechanisms of movement recovery, however, were still not clear. I gained an opportunity to investigate this question in animal models of stroke under the supervision of Dr. Eric Rouiller. The main topic of my research was to study the anatomical neuronal regeneration in association with functional recovery after cortical lesions in adult primates. The mechanistic studies in primates intrigued me and encouraged me to explore neural mechanisms of recovery in adult humans using noninvasive techniques. To this effect, I am currently utilizing electrophysiological approaches, such as Electroencephalography (EEG) and Electromyography (EMG), along with neurophysiological stimulation methods including transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS), as well as neuroimaging with functional magnetic resonance imaging, (fMRI). This ongoing research is being conducted under the mentorship and supervision of Dr. Alvaro Pascual-Leone at the Berenson-Allen Center for Noninvasive Brain Stimulation at Beth Israel Deaconess Medical Center and Harvard Medical School. The preliminary results of our projects suggest that individuals with neurological disorders exhibit plasticity of surviving networks and that the degree of plasticity is highly correlated with behavioral measures and functional recovery. These findings provide an understanding of the potential mechanisms involving neuronal systems as well as other long-range cortical circuits. Furthermore, these insights offer possible new therapeutic targets, harnessing plasticity to promote recovery from ASD etc. The graduate and post-doctoral experience in my research career has helped me progress towards my overall goal of becoming an independent and successful researcher in the area of clinical neuroscience and interventional neurophysiology involving patients with neurological disorders. Innovation in my research involves the combination of research tools and techniques (e.g. simultaneous electrophysiological recording and TMS) to provide data that any one technique cannot provide on its

7 own. Furtheremore, I am to bridge levels of analyses by collaborating with molecular and genetic neuroscientists to further understand how the system’s level mechanisms are controlled by more fundamental processes.

Teaching Over the course of my career in academic research I have taught formally and informally. While completing my PhD work at the University of Fribourg, Switzerland, I taught in the Neurophysiology practical course to second year medical students. More recently, since 2008, when I joined the Berenson- Allen Center for Noninvasive Brain Stimulation, I have been involved as faculty of the Intensive Course in Transcranial Magnetic Stimulation. This is a continuing medical education course through Harvard Medical School that is offered through the Center for Noninvasive Brain Stimulation at Beth Israel Deaconess Medical Center. This course focuses on the theory and practical applications of TMS and is offered to a wide international audience of clinical and basic researchers. My involvement has been focused on the discussion of the safety aspects of the application of TMS, as well as the practical demonstration of the use of navigated TMS based on principles of MRI-guided frameless stereotaxy to deliver TMS over desired locations with great spatial precision.