Table S1. Primers and PCR Parameters of the Genes Included in the Study
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Table S1. Primers and PCR parameters of the genes included in the study.
Gene/ primers Primers F/R: 5´- 3´ Ta [MgCL] mM Chr Nuc cyt b L14729 GACATGAAAAATCATCGTTGTTATT 60 2.0 mtDNA 1143 H15985 TAGAATGTCAGCTTTGGGTGCT
LCLE1 ATCATCAACCACKCATTTATT 46.1 1.2
MCLE3 ATTATGCCTGCTATTGGTATG
LCAT CACCATCTTCCTGGATCTCAA 58 2 8 563
AAGAAATACAGCACATGTAGGCA
G6pd CAGATCTGTGAACGTGTTTGG 58 2.5 X 577
GGTACAACTCTTCCCTCAGG
BRCA1 AAGACAGAATGTAGAAAAGGCTG 50.7 2.5 11 576
ACCGACAGGCTCAAGTGCGA
GHR GGCGTTCATGACAACTACAAACCTGA 62 1.5 15 617
ATAGCCACACGAGGAGAGGAACT vWF CTTCCTGCTGGACGGCTCCT 62 2 6 846
TTAGGGGCCTGCTTCCGGTC
ADRAB2 GCAGCCGCGGACATCCTAGT 61 1.5 2 808
CGCCAATGACCACTGCCAGC cyt b - cytochrome b, LCAT - lecithin-cholesterol acyltransferase, G6pd - Glucose-6-phosphate dehydrogenase, BRCA1
- Breast cancer 1 gene, GHR - growth hormone receptor, vWF - von Willebrand factor, ADRAB2 - alpha 2B adrenergic receptor, Ta – annealing temperature, [MgCL] - MgCl concentration, Chr - chromosome assignments for Microtus species, Nuc - length of sequence.