Supplemental Table 1. the Effects of CH4 Ventilation on Arterial Ph, Arterial O2 Saturation

Supplemental Table 1. The effects of CH4 ventilation on arterial pH, arterial O2 saturation

(spaO2), partial arterial O2 pressure (paO2) [mmHg], and partial arterial CO2 pressure (paCO2) [mmHg] in rats.

Groups 0 min 60 min 90 min 240 min

Median 7.44 7.47 7.45 7.46 R1 25p; 75p 7.43; 7.44 7.46; 7.47 7.44; 7.47 7.446; 7.482 Median 7.44 7.47 7.46 7.45 R2 25p; 75p 7.40; 7.48 7.45; 7.48 7.45; 7.49 7.43; 7.48 Median 7.45 7.48 7.49 7.46 R3 25p; 75p 7.42; 7.47 7.45; 7.49 7.44; 7.54 7.41; 7.50 Median 7.44 7.45 7.44 7.40 R4 25p; 75p 7.40; 7.47 7.41; 7.48 7.41; 7.46 7.37; 7.43 Median 7.40 7.42 7.42 7.46 R5 25p; 75p 7.37; 7.43 7.40; 7.44 7.38; 7.45 7.44; 7.49 Median 7.4 7.44 7.40 7.49 R6 25p; 75p 7.37; 7.41 7.42; 7.45 7.35; 7.45 7.45; 7.52

paO2 [mmHg]

Median 71.59 58.68 65.93 61.48 R1 25p; 75p 65.00; 73.90 54.10; 63.00 58.58; 69.55 56.95; 72.38 Median 82.60 64.92 63.40 62.05 R2 25p; 75p 76.67; 88.97 59.07; 69.80 63.10; 66.80 54.17; 69.12 Median 81.13 56.82 57.00 48.00 * R3 25p; 75p 78.12; 87.52 50.52; 61.00 51.60; 61.88 45.72; 48.13 Median 86.10 57.20 51.67 51.55 * R4 25p; 75p 78.22; 86.95 52.97; 60.67 48.90; 56.80 46.20; 55.30 Median 81.73 43.35 + 34.10 * # + 39.50 * # + R5 25p; 75p 76.77; 89.12 35.07; 50.10 33.83; 40.55 36.62; 42.72 Median 81.78 43.40 + 37.72 * 40.10 * # + R6 25p; 75p 76.47; 86.40 40.30 51.67 34.80; 50.30 36.35; 42.37

paCO2 [mmHg]

Median 38.65 33.95 33.78 31.40 R1 25p; 75p 37.50; 39.30 32.90 34.70 31.60; 38.53 29.40; 33.00 Median 28.60 # 24.08 # 21.79 # 20.92 * # R2 25p; 75p 25.80; 31.70 22.97; 25.50 20.00; 22.30 19.52; 22.70

1 Median 37.50 + 32.18 + 30.60 * + 28.50 * + R3 25p; 75p 35.32; 39.08 28.57; 36.42 29.99; 30.68 25.90; 32.84 Median 30.23 26.30 27.38 24.20 * R4 25p; 75p 27.85; 31.95 23.90; 29.01 26.00; 28.50 23.40; 26.20 Median 33.20 29.10 29.20 28.02 + R5 25p; 75p 32.07; 38.08 28.85; 29.67 27.13; 31.65 26.35; 30.02 Median 33.32 25.23 * # 26.94 25.82 * R6 25p; 75p 30.05; 36.45 22.82; 27.45 21.90; 28.90 23.85; 27.07

spO2

Median 94.48 91.71 93.4 92.60 R1 25p; 75p 93.50; 95.80 89.60; 93.60 90.93; 94.80 91.10; 94.50 Median 97.25 94.70 95.20 * 93.95 R2 25p; 75p 96.72; 98.15 93.76; 96.62 93.60; 95.50 92.17; 96.02 Median 96.40 90.70 91.90 85.70 * R3 25p; 75p 95.96; 97.17 87.37; 93.45 89.21; 92.90 83.69; 86.27 Median 97.50 92.35 91.19 90.52 * R4 25p; 75p 96.83; 98.17 91.55; 93.75 88.20; 92.30 85.00; 92.80 Median 96.60 81.87 + 73.10 * # + 78.02 * # + R5 25p; 75p 95.27; 97.85 73.80; 90.32 68.35; 81.30 75.75; 80.27 Median 96.92 85.62 + 76.06 * + 77.72 * + R6 25p; 75p 96.50; 97.67 80.92; 90.07 72.70; 89.80 72.57; 85.10

The animals were mechanically ventilated with room air in the first 10 min of the protocol. The respiration was then continued from the gas-bottles containing artificial air with different percentages of O2 with or without 2.5% CH4. Group R1 (n=6) was ventilated with air containing 21% O2; group R2 (n=6) with 21% O2 + 2.5% CH4; group R3 (n=6) air containing

18.5% O2; group R4 (n=6) with 18.5% O2 + 2.5% CH4; group R5 (n=6) with air containing

th th 16.5% O2; group R6 (n=6) with 16.5% O2 + 2.5% CH4. Median values and 75 and 25 percentiles are given. p < 0.05 was considered statistically significant. *p < 0.05 relative to the baseline. #p < 0.05 relative to group R1. +p < 0.05 relative to the group R2. @p < 0.05 relative to group R3.