% Glucose Uptake

Supplementary 1

% Glucose uptake

v1: GLC <-> G6P abcdef <-> abcdef

% EMP-pathway v2: G6P <-> F6P abcdef <-> abcdef

v3: F6P <-> G3P + G3P abcdef <-> cba + def

v4: G3P <-> PEP abc <-> abc

v5: PEP <-> PYRCYT abc <-> abc

% PP-pathway and Phosphoketolase

v6: G6P <-> CO + P5P abcdef <-> a + bcdef

vx1: P5P <-> G3P + ACE abcde <-> abc + de

v7: P5P + P5P <-> S7P + G3P abcde + fghij <-> fgabcde + hij v8: S7P + G3P <-> F6P + E4P abcdefg + hij <-> abchij + defg

v9: P5P + E4P <-> F6P + G3P abcde + fghi <-> abfghi + cde

% Formation of AcCoA in the cytosol

v14: ACE <-> ACCOACYT ab <-> ab

% Anaplerotic reaction (cytosolic)

v15: PYRCYT + CO <-> OAACYT abc + d <-> abcd

% TCA-cycle

v16: PYRMIT <-> ACCOAMIT + CO abc <-> bc + a

v17: OAAMIT + ACCOAMIT <-> ICIT abcd + ef <-> dcbafe

v18: ICIT <-> AKG + CO abcdef <-> abcef + d

v19: AKG <-> FUM + CO abcde <-> bcde + a

% variable scrambling fraction

v20: FUM + FUM <-> OAAMIT + OAAMIT abcd + efgh <-> abcd + hgfe

v21: OAAMIT <-> FUM abcd <-> abcd

% Transports

v22: OAAMIT <-> OAACYT abcd <-> abcd

v24: PYRCYT <-> PYRMIT abc <-> abc

% Threonine / Serine / Glycine metabolism (all enzymes assumed to be cytosolic)

v25: G3P <-> SER abc <-> abc

v26: SER <-> GLY + C1 abc <-> ab + c

v27: OAACYT <-> THR abcd <-> abcd

v28: THR <-> GLY + ACA abcd <-> ab + cd

% Malic enzyme (oxaloacetate decarboxylation, mitochondrial)

v29: OAAMIT <-> PYRMIT + CO abcd <-> abc + d

% Drain of intermediates to macromolecules

v30: G6P <-> G6POUT abcdef <-> abcdef

v31: P5P <-> P5POUT abcde <-> abcde

v32: E4P <-> E4POUT abcd <-> abcd

v33: G3P <-> G3POUT abc <-> abc

v34: PEP <-> PEPOUT abc <-> abc

v35: PYRMIT <-> PYRMITOUT abc <-> abc

v36: PYRCYT <-> PYRCYTOUT abc <-> abc v37: OAACYT <-> OAACYTOUT abcd <-> abcd

v38: AKG <-> AKGOUT abcde <-> abcde

v39: ACCOACYT <-> ACCOACYTOUT ab <-> ab

v40: ACCOAMIT <-> ACCOAMITOUT ab <-> ab

v41: SER <-> SEROUT abc <-> abc

v42: GLY <-> GLYOUT ab <-> ab

v43: C1 <-> C1OUT a <-> a

v44: THR <-> THROUT abcd <-> abcd

net 0.0 200.0; exch = 0.0;

% CO2-evolution

v48: CO2 <-> CO2OUT a <-> a