Altered Expression of Multiple Genes Involved in Retinoic Acid Biosynthesis in Human Colorectal

Altered Expression of Multiple Genes Involved in Retinoic Acid Biosynthesis in Human Colorectal Cancer Pathology and Oncology Research journal Ekaterina S. Kropotova, Olga L. Zinovieva, Alisa F. Zyryanova, Vera I. Dybovaya, Vladimir S. Prasolov, Sergey F. Beresten, Nina Yu. Oparina, Tamara D. Mashkova Corresponding author: Tamara D. Mashkova, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia; E-mail address: [email protected]

Supplementary Table S4 Description of primers

Commonly used gene Official gene Primers (5’- 3’) Product size, NCBI Refseq mRNA symbol symbol b.p. ID

F GGGTGACTACAGTCAAACCAGGTGA ADH1A ADH1A R GGTCCCCTGAGGATTGCTTACATCG 131 NM_000667.3 (class I) F TGGCACCAGCACCTTCTCCC ADH1B (class I) ADH1B R GAGCCTGGGGTGACCTTGGC 156 NM_000668.4

F AAGACAGAATCAATATGAGCACAGCAGG ADH1C (class I) ADH1C R ACCAGGTTGCCACTAACCACATGC 190 NM_000669.3

F TGCAATCAACAATGCCAAGGTCACCC ADH2 ADH4 R CAGTCAGTGGCTCCCAGGGC 174 NM_000670.3 (class II) F GCTGGTGCTTCCCGGATCATTGG ADH3 (class III) ADH5 R GCCTCAAGTGCTGCTCTCATGACC 203 NM_000671.3

F GACCAGCGTGGTTGTAGGAGTTCC ADH4 (class IV) ADH7 R TCCATGTGCGTCCAGTGAAGAGC 83 NM_001166504.1

F TGGAAACTTGGCAGCCAGAA RDHL DHRS9 R CCAGAGACCTTTCTCCCCAA 199 NM_001142271.1

F TTCTCTGACAGCCTGAGGCG RDH5 RDH5 R TGCGCTGTTGCATTTTCAGG 202 NM_001199771.1

F AATAATGCTGGTGTGGTCTCTGG RDH10 RDH10 R CTGGCACAGTAATCCTCAACTCC 209 NM_172037.3

F CACTTGGGTCACTTCCTCCTAAC RDH11 RDH11 R AACGCCAGAGCCTTTTAGTCTC 228 NM_016026.3

F TGGAACGATGCTGGTCACCTTGG RDH12 RDH12 R GATCACCACTACCTTGCCAGGAAGC 139 NM_152443.2

F GACCGGTCCAGTCCAGAGGTC RoDH4 RDH16 R TAGCGAGTACGGGGGTGGCAG 161 NM_003708.3

F TGGATGAAGGGCTGGTGAA AKR1B1 AKR1B1 R GGTGGCACTCAATCTGGTTA 121 NM_001628.2

F GGACCTGTTCATCGTCAGCAA AKR1B10 AKR1B10 R CCCCAGACTTGAATCCCTGTG 145 NM_020299.4

F AACTCCTCTCACTGCTCTCCACG RALDH1 ALDH1A1 R GTCACCCTCTTCAGATTGCTTTTCC 210 NM_000689.4

F CAAGATGTCTGGAAATGGGAGAG RALDH2 ALDH1A2 R CTTTAAGTAAGGACCGTGGCTCA 210 NM_001206897.1

F TCAACTGCTACAACGCCCTCTAT RALDH3 ALDH1A3 R CGCCGTCCGATGTTTGAG 184 NM_000693.2

F GCTGTACCGGGGCATGAAGGC CYP26A1 CYP26A1 R CAGCCGCTCTCCCCTCTCC 130 NM_057157.2

F TAGAGATGAGAGTGGAAGGTGTGGT CRBP1 RBP1 R GGGGTGGCTGGACATTTTTG 153 NM_002899.3

F TATCCAGCGTACTCCAAAGA B2M B2M R GACAAGTCTGAATGCTCCAC 165 NM_004048.2

F CCTTCCTGGGCATGGAGTC ACTB ACTB R CTTGATCTTCATTGTGCTGGGT 191 NM_001101.3 Shown are commonly used gene symbols applied in this paper, official gene symbols, primer sequences, PCR product sizes and NCBI Refseq mRNA IDs. Abbreviations: ADH1A, ADH1B, ADH1C, ADH2, ADH3 and ADH4 alcohol dehydrogenases; RDHL, RDH5, RDH10, RDH11, RDH12, RoDH4 retinol dehydrogenases; AKR1B1 and AKR1B10 aldo-keto reductases; RALDH1, RALDH2 and RALDH3 retinaldehyde dehydrogenases; CYP26A1 cytochrome P450 26A1, CRBP1 cellular retinol-binding protein, ACTB beta-actin, β2M beta2-microglobulin