Cytotoxic Activity of Eumitrin A1 from Lichen Usnea Blepharea Motyka

Cytotoxic Activity of Eumitrin A1 From Lichen Usnea blepharea Motyka

Maulidiyah*1, Herry Cahyana2, Wahyudi Priyono Suwarso2, Muhammad Nurdin1 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Halu Oleo, Jl. H.E.A. Mokodompit Kampus Baru, Kendari 93232 – Southeast Sulawesi, Indonesia 2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok – West Java 16424, Indonesia

*Corresponding Autor. Telp. E-mail: [email protected]

The purpose of this study was to isolate and elucidate structure of secondary metabolite compounds from the lichen Usnea blepharea Motyka that was obtained from Sulawesi – Indonesia, then performed a bioactivity test against Murine Leukemia P388 cells. Powder of lichen has extracted using an acetone solvent and thin layer chromatography (TLC), that continued with separation by using column chromatography (CC) with n-hexane and ethyl acetate solvents in a gradient to produce of pure isolates. The structure was determined by using spectroscopy i.e. UV-Vis, LC-MS, FTIR, 1H-NMR, 13C-NMR, 2D-NMR (HMBC, HMQC, COSY). The results obtained using UV-Vis spectrophotometer gives max wavelength of 229 nm and 334 nm, respectively. From LC-MS data this compound has molecule weight of 680. 1H-NMR, 13C-NMR with DEPT spectrums showed this compound has 34 carbon atoms consisting of 2 methyl groups, 2 methoxy groups, 1 methyl group, 6 metin groups, 3 methylene groups, and 20 C quartener atoms. Supported by using 2D-NMR (HMBC, HMQC, COSY) spectrums the result of analysis can be inferred this compound is a Eumitrin A1 with the formula structure C34H32O15. The result of cytotoxic activity test against Murine Leukemia P388 cells showed very active with IC50 is 4.5 µg/mL.

Biography

Maulidiyah has completed her PhD at the University of Indonesia, Jakarta. She is the Head of Organic Laboratory at the Universitas Halu Oleo, Kendari, Indonesia. She has published more than 15 papers in reputed journals and is working as a Lecturer at Universitas Halu Oleo, Province of Southeast Sulawesi, Indonesia.

Presenting author details Full name : Dr. Hj. Maulidiyah, M.Si. Contact number : +6281388327118 Twitter account : - Linked In account : - Session name/ number: - Category: (Oral presentation)