Correlation Force Spectroscopy for Single Molecule Measurements

Total Page：16

File Type：pdf, Size：1020Kb

springer.com Chemistry : Spectroscopy / Spectrometry Radiom, Milad Correlation Force Spectroscopy for Single Molecule Measurements Nominated as an outstanding theses by Virginia Tech Introduces the dynamic new technique for correlation force spectroscopy (CFS) Details the significance of CFS in polymer physics and chemistry Explains the measurement capability in the area of high frequency rheology and single molecule dynamic measurements This thesis addresses the development of a new force spectroscopy tool, correlation force Springer spectroscopy (CFS) for the measurement of the properties of very small volumes of material 2015, XXVII, 117 p. 48 (molecular to µm3) at kHz-MHz frequency range. CFS measures the simultaneous thermal 1st illus., 31 illus. in color. edition fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and Printed book micro-rheology. Hardcover Printed book Order online at springer.com/booksellers Hardcover Springer Nature Customer Service Center LLC ISBN 978-3-319-14047-6 233 Spring Street $ 139,99 New York, NY 10013 USA Available T: +1-800-SPRINGER NATURE Discount group (777-4643) or 212-460-1500 Professional Books (2) [email protected] Product category Monograph Series Springer Theses Other renditions Softcover ISBN 978-3-319-38640-9 Softcover ISBN 978-3-319-14049-0 Prices and other details are subject to change without notice. All errors and omissions excepted. Americas: Tax will be added where applicable. Canadian residents please add PST, QST or GST. Please add $5.00 for shipping one book and $ 1.00 for each additional book. Outside the US and Canada add $ 10.00 for first book, $5.00 for each additional book. If an order cannot be fulfilled within 90 days, payment will be refunded upon request. Prices are payable in US currency or its equivalent. ISBN 978-3-319-14047-6 / BIC: PNFS / SPRINGER NATURE: SCC11020 Part of .

Copy Link

Recommended publications

Recent Applications of Advanced Atomic Force Microscopy in Polymer Science: a Review
polymers Review Recent Applications of Advanced Atomic Force Microscopy in Polymer Science: A Review Phuong Nguyen-Tri 1,2,*, Payman Ghassemi 2, Pascal Carriere 3, Sonil Nanda 4 , Aymen Amine Assadi 5 and Dinh Duc Nguyen 6,7 1 Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam 2 Département de Chimie, Biochimie et Physique, Université du Québec à Trois-Rivières (UQTR), Trois-Rivières, QC G8Z 4M3, Canada; [email protected] 3 Laboratoire MAPIEM (EA 4323), Matériaux Polymères Interfaces Environnement Marin, Université de Toulon, CEDEX 9, 83041 Toulon, France; [email protected] 4 Department of Chemical and Biological Engineering, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada; [email protected] 5 ENSCR—Institut des Sciences Chimiques de Rennes (ISCR)—UMR CNRS 6226, Univ Rennes, 35700 Rennes, France; [email protected] 6 Faculty of Environmental and Food Engineering, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City 755414, Vietnam; [email protected] 7 Department of Environmental Energy Engineering, Kyonggi University, Suwon 16227, Korea * Correspondence: [email protected]; Tel.: +819-376-5011 (ext. 4505) Received: 5 March 2020; Accepted: 13 May 2020; Published: 17 May 2020 Abstract: Atomic force microscopy (AFM) has been extensively used for the nanoscale characterization of polymeric materials. The coupling of AFM with infrared spectroscope (AFM-IR) provides another advantage to the chemical analyses and thus helps to shed light upon the study of polymers. This paper reviews some recent progress in the application of AFM and AFM-IR in polymer science.
[Show full text]
Quantum Limit in Subnanometre-Gap Tip-Enhanced Nanoimaging of Few
Quantum limit in subnanometre-gap tip-enhanced nanoimaging of few-layer MoS2 Yingchao Zhang1,2*, Dmitri V. Voronine1,3*#, Shangran Qiu1,2, Alexander M. Sinyukov1, Mary Hamilton3, Alexei V. Sokolov1,3, Zhenrong Zhang3 and Marlan O. Scully1,3,4# 1Texas A&M University, College Station, TX 77843, USA 2Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China 3Baylor University, Waco, TX 76798, USA 4Princeton University, Princeton, New Jersey 08544, USA Two-dimensional (2D) materials beyond graphene such as transition metal dichalcogenides (TMDs) have unique mechanical, optical and electronic properties with promising applications in flexible devices, catalysis and sensing. Optical imaging of TMDs using photoluminescence and Raman spectroscopy can reveal the effects of structure, strain, doping, defects, edge states, grain boundaries and surface functionalization. However, Raman signals are inherently weak and so far have been limited in spatial resolution in TMDs to a few hundred nanometres which is much larger than the intrinsic scale of these effects. Here we overcome the diffraction limit by using resonant tip-enhanced Raman scattering (TERS) of few-layer MoS2, and obtain nanoscale optical images with ~ 20 nm spatial resolution. This becomes possible due to electric field enhancement in an optimized subnanometre-gap resonant tip-substrate configuration. We investigate the limits of signal enhancement by varying the tip-sample gap with sub-Angstrom precision and observe a quantum quenching behavior, as well as a Schottky-Ohmic transition, for subnanometre gaps, which enable surface mapping based on this new contrast mechanism. This quantum regime of plasmonic gap-mode enhancement with a few nanometre thick MoS2 junction may be used for designing new quantum optoelectronic devices and sensors.
[Show full text]
Curved-Mechanical Characteristic Measurements of Transparent Conductive Film-Coated Polymer Substrates Using Common-Path Optical Interferometry
coatings Article Curved-Mechanical Characteristic Measurements of Transparent Conductive Film-Coated Polymer Substrates Using Common-Path Optical Interferometry Bor-Jiunn Wen * and Jui-Jen Hsu Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan; [email protected] * Correspondence: [email protected] Abstract: This study proposes a method for measuring curved-mechanical characteristics based on a whole-folding test for transparent conductive film-coated polymer substrates using common- path optical interferometry. Accordingly, 80-, 160-, and 230-nm indium tin oxide films coated on 40 × 40 mm 125-µm-thick polyethylene terephthalate (PET) substrates, and monolayer graphene films coated on 40 × 40 mm 250-µm-thick PET substrates are inspected and analyzed under the curving conditions of 50-, 30-, 20-, and 10-mm radii before and after an 11,000 whole-folding cycle test based on a 10-mm folding radius. This study utilizes the changes in the phase retardations of transparent conductive film-coated polymer substrates under different curving conditions before and after 11,000 whole-folding cycles to analyze the substrates’ residual stress characteristics that were the direct result of manufacturing process parameters. The results from this study of curved-mechanical characteristic measurements of flexible transparent conductive substrates can provide designers Citation: Wen, B.-J.; Hsu, J.-J. Curved-Mechanical Characteristic with improved product development and can assist manufacturers in improving the manufacturing Measurements of Transparent design of enhanced coating processes. Conductive Film-Coated Polymer Substrates Using Common-Path Keywords: curved-mechanical characteristic measurements; whole-folding test; transparent Optical Interferometry. Coatings 2021, conductive film-coated polymer substrate; common-path optical interferometry 11, 766.
[Show full text]
Polymer Characterization with the Atomic Force Microscope
Chapter 4 Polymer Characterization with the Atomic Force Microscope U. Maver, T. Maver, Z. Peršin, M. Mozetič, A. Vesel, M. Gaberšček and K. Stana-Kleinschek Additional information is available at the end of the chapter http://dx.doi.org/10.5772/51060 1. Introduction 1.1. Atomic force microscopy Atomic force microscopy is a powerful characterization tool for polymer science, capable of revealing surface structures with superior spatial resolution [1]. The universal character of repulsive forces between the tip and the sample, which are employed for surface analysis in AFM, enables examination of even single polymer molecules without disturbance of their integrity [2]. Being initially developed as the analogue of scanning tunneling microscopy (STM) for the high-resolution profiling of non-conducting surfaces, AFM has developed into a multifunctional technique suitable for characterization of topography, adhesion, mechanical, and other properties on scales from tens of microns to nanometers [3]. 1.2. The technique A schematic representation of the basic AFM setup is shown in Figure 1. Using atomic force microscopy (AFM), a tip attached to a flexible cantilever will move across the sample surface to measure the surface morphology on the atomic scale. The forces between the tip and the sample are measured during scanning, by monitoring the deflection of the cantilever [1]. This force is a function of tip sample separation and the material properties of the tip and the sample. Further interactions arising between the tip and the sample can be used to investigate other characteristics of the sample, the tip, or the medium in-between [4]. 1.2.1.
[Show full text]
(ATR–FTIR) Spectroscopy, Micro–Attenuated Total Reflectance Fourier
Organic and Medicinal Chemistry International Journal ISSN 2474-7610 Image Article Organic & Medicinal Chem IJ Volume 6 Issue 1 - March 2018 Copyright © All rights are reserved by Alireza Heidari DOI: 10.19080/OMCIJ.2018.06.555677 Fourier Transform Infrared (FTIR) Spectroscopy, Attenuated Total Reflectance Fourier Transform Infrared (ATR–FTIR) Spectroscopy, Micro–Attenuated Total Reflectance Fourier Transform Infrared (Micro–ATR–FTIR) Spectroscopy, Macro–Attenuated Total Reflectance Fourier Transform Infrared (Macro–ATR–FTIR) Spectroscopy, Two–Dimensional Infrared Correlation Spectroscopy, Linear Two–Dimensional Infrared Spectroscopy, Non–Linear Two– Dimensional Infrared Spectroscopy, Atomic Force Microscopy Based Infrared (AFM–IR) Spectroscopy, Infrared Photodissociation Spectroscopy, Infrared Correlation Table Spectroscopy, Near– Infrared Spectroscopy (NIRS), Mid–Infrared Spectroscopy (MIRS), Nuclear Resonance Vibrational Spectroscopy, Thermal Infrared Spectroscopy and Photothermal Infrared Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time Alireza Heidari* Faculty of Chemistry, California South University, USA Submission: February 26, 2018; Published: March 29, 2018 *Corresponding author: Alireza Heidari, Faculty of Chemistry, California South University, 14731 Comet St. Irvine, CA 92604, USA, Email: Abbreviations: FTIR : Fourier Transform Infrared; ATR-FTIR: Attenuated Total Reflectance Fourier Transform Infrared; Micro-ATR-FTIR: Micro- Attenuated
[Show full text]
Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature
sensors Article Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature Reinier Oropesa-Nuñez 1,† , Andrea Mescola 2,† , Massimo Vassalli 3,* and Claudio Canale 4 1 Department of Materials Science and Engineering, Uppsala University, Ångströmlaboratoriet, Box 35, SE-751 03 Uppsala, Sweden; [email protected] 2 CNR-Nanoscience Institute-S3, Via Campi 213/A, 41125 Modena, Italy; [email protected] 3 James Watt School of Engineering, University of Glasgow, Glasgow G128LT, UK 4 Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy; canale@ﬁsica.unige.it * Correspondence: [email protected] † These authors contributed equally to the paper. Abstract: Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imag- ing resolution, and also offering the possibility to probe interaction forces down to few pN. Recently, this technique has been specialized to study the interaction between single living cells, one on the substrate, and a second being adhered on the cantilever. Cell–cell force spectroscopy offers a unique tool to investigate in ﬁne detail intra-cellular interactions, and it holds great promise to elucidate elusive phenomena in physiology and pathology. Here we present a systematic study of the effect of the main measurement parameters on cell–cell curves, showing the importance of controlling the experimental conditions. Moreover, a simple theoretical interpretation is proposed, based on the number of contacts formed between the two interacting cells. The results show that single cell–cell force spectroscopy experiments carry a wealth of information that can be exploited to understand the inner dynamics of the interaction of living cells at the molecular level.
[Show full text]
AFM-Based Force Spectroscopy Guided by Recognition Imaging: a New Mode for Mapping and Studying Interaction Sites at Low Lateral Density
Protocol AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density Melanie Koehler 1,2 , Anny Fis 1, Hermann J. Gruber 1 and Peter Hinterdorfer 1,* 1 Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria; [email protected] (M.K.); anny.ﬁ[email protected] (A.F.); [email protected] (H.J.G.) 2 Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, 1348 Louvain-La-Neuve, Belgium * Correspondence: [email protected]; Tel.: +43-732-2468-7631 Received: 19 October 2018; Accepted: 3 January 2019; Published: 8 January 2019 Abstract: Ligand binding to receptors is one of the most important regulatory elements in biology as it is the initiating step in signaling pathways and cascades. Thus, precisely localizing binding sites and measuring interaction forces between cognate receptor–ligand pairs leads to new insights into the molecular recognition involved in these processes. Here we present a detailed protocol about applying a technique, which combines atomic force microscopy (AFM)-based recognition imaging and force spectroscopy for studying the interaction between (membrane) receptors and ligands on the single molecule level. This method allows for the selection of a single receptor molecule reconstituted into a supported lipid membrane at low density, with the subsequent quantiﬁcation of the receptor–ligand unbinding force. Based on AFM tapping mode, a cantilever tip carrying a ligand molecule is oscillated across a membrane. Topography and recognition images of reconstituted receptors are recorded simultaneously by analyzing the downward and upward parts of the oscillation, respectively.
[Show full text]
Review Recent Progressive Use of Advanced Atomic Force Microscopy in Polymer Science: a Review
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 5 March 2020 doi:10.20944/preprints202003.0082.v1 Peer-reviewed version available at Polymers 2020, 12, 1142; doi:10.3390/polym12051142 Review Recent progressive use of advanced atomic force microscopy in polymer science: A review Phuong Nguyen-Tri 1*, Payman Ghassemi1, Pascal Carriere2, Aymen Amine Assadi3, Dinh Duc Nguyen4 1. Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières (UQTR), Québec, G8Z 4M3, Canada. Email : [email protected]; [email protected] 2. Laboratoire MAPIEM (EA 4323), Matériaux Polymères Interfaces Environnement Marin, Université de Toulon, Toulon, France. Email : [email protected] 3. Univ Rennes, ENSCR- Institut des Sciences Chimiques de Rennes (ISCR) - UMR CNRS 6226, France. [email protected] 4. Department of Environmental Energy Engineering, Kyonggi University, Suwon, Republic of Korea, email: [email protected] * Correspondence: [email protected]; Tel.: +819-376 5011 (ext. 4505) Abstract: Atomic force microscopy (AFM) has been extensively used for the nanoscale characterization of polymeric materials. The coupling of AFM with infrared spectroscope (AFM-IR) provides another advantage to the chemical analyses and thus helps to shed light upon the study of polymers. In this perspective paper, we review recent progress in the use of AFM-IR in polymer science. We describe first the principle of AFM-IR and the recent improvements to enhance its resolution. We discuss then the last progress in the use of AFM-IR as a super-resolution correlated scanned-probe IR spectroscopy for chemical characterization of polymer materials dealing with polymer composites, polymer blends, multilayers and biopolymers.
[Show full text]
Single-Molecule Chemistry with Surface- and Tip-Enhanced Raman Spectroscopy † ‡ † † † Alyssa B
Review pubs.acs.org/CR Single-Molecule Chemistry with Surface- and Tip-Enhanced Raman Spectroscopy † ‡ † † † Alyssa B. Zrimsek, Naihao Chiang, Michael Mattei, Stephanie Zaleski, Michael O. McAnally, † † † † ‡ § Craig T. Chapman, Anne-Isabelle Henry, George C. Schatz, and Richard P. Van Duyne*, , , † ‡ § Department of Chemistry, Applied Physics Program, and Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States ABSTRACT: Single-molecule (SM) surface-enhanced Raman spectroscopy (SERS) and tip-enhanced Raman spectroscopy (TERS) have emerged as analytical techniques for characterizing molecular systems in nanoscale environments. SERS and TERS use plasmonically enhanced Raman scattering to characterize the chemical information on single molecules. Additionally, TERS can image single molecules with subnanometer spatial resolution. In this review, we cover the development and history of SERS and TERS, including the concept of SERS hot spots and the plasmonic nanostructures necessary for SM detection, the past and current methodologies for verifying SMSERS, and investigations into understanding the signal heterogeneities observed with SMSERS. Moving on to TERS, we cover tip fabrication and the physical origins of the subnanometer spatial resolution. Then, we highlight recent advances of SMSERS and TERS in ﬁelds such as electrochemistry, catalysis, and SM electronics, which all beneﬁt from the vibrational characterization of single molecules. SMSERS and TERS provide new insights on molecular behavior that would otherwise be obscured in an ensemble- averaged measurement. CONTENTS 3. Single-Molecule and High-Resolution TERS P 3.1. Background and Principles P 1. Introduction B 3.2. AFM and STM Tip Fabrication R 1.1. Background B 3.3. Spatial Resolution S 1.2. Scope of Review C 3.3.1.
[Show full text]
Atomic Force Microscopy-Based Characterization and Design of Biointerfaces
REVIEWS Atomic force microscopy-based characterization and design of biointerfaces David Alsteens1, Hermann E. Gaub2, Richard Newton3, Moritz Pfreundschuh3, Christoph Gerber4 and Daniel J. Müller3 Abstract | Atomic force microscopy (AFM)-based methods have matured into a powerful nanoscopic platform, enabling the characterization of a wide range of biological and synthetic biointerfaces ranging from tissues, cells, membranes, proteins, nucleic acids and functional materials. Although the unprecedented signal-to-noise ratio of AFM enables the imaging of biological interfaces from the cellular to the molecular scale, AFM-based force spectroscopy allows their mechanical, chemical, conductive or electrostatic, and biological properties to be probed. The combination of AFM-based imaging and spectroscopy structurally maps these properties and allows their 3D manipulation with molecular precision. In this Review, we survey basic and advanced AFM-related approaches and evaluate their unique advantages and limitations in imaging, sensing, parameterizing and designing biointerfaces. It is anticipated that in the next decade these AFM-related techniques will have a profound influence on the way researchers view, characterize and construct biointerfaces, thereby helping to solve and address fundamental challenges that cannot be addressed with other techniques. Surfaces at which tissues, microorganisms, cells, viruses and multiparametric characterization of biointerfaces or biomolecules make contact with other natural or syn- in their native state or in an environment that simu- thetic materials are termed biointerfaces. Understanding lates physiological conditions, which is of particular and manipulating the sensing and interactions that occur importance to understand how tissues, cells and bio- at biointerfaces is an enterprise common to a host of sci- molecules function.
[Show full text]
Force Spectroscopy with Optical and Magnetic Tweezers
2 Force Spectroscopy with Optical and Magnetic Tweezers Richard Conroy 1 Introduction Micromanipulation of individual cells and molecules is increasingly important for a wide range of biophysical research because, although ensemble biochemical analysis pro- vides excellent qualitative and quantitative descriptions, it seldom describes phenomena at the molecular level. By observing the force spectroscopy of single molecules, the kinetics, mechanics, and variation of structure, function, and interactions can be fully explored to pro- vide a more complete physiological picture. The use of electric and magnetic fields for manipulating particles dates back more than a century, with a rich tapestry of applications in separation, filtering and trapping. Recognizing the non-contact advantages of magnetic manipulation, Crick and Hughes probed the physical properties of a cell’s cytoplasm more than fifty years ago using mag- netic particles [1]. Two decades later, with the development of intense electromagnetic fields from lasers, the manipulation of latex particles with light was experimentally dem- onstrated by Ashkin in 1970 in his “levitation traps” [2]. Ashkin went on to pioneer optical trapping of both atoms and biomolecules, leading to one of the most successful technology transfers from a physics lab to cell biology. For many applications, in particular for characterizing biomolecules and their inter- actions, it is desirable to have a non-contact technique for exerting a force. A non-contact technique allows the behavior of a single molecule under stretching or torsional forces to be measured and manipulated without complicating surface effects or material response limita- tions. Non-contact techniques also benefit from being easier to multiplex into exerting force on multiple sites of the same molecule or multiple heterogeneous molecules, or to collect parallel statistics on homogeneous copies of the same system.
[Show full text]
Abstracts for ICP2019
The 29th International Conference on Photochemistry Boulder, Colorado • July 21 – 26, 2019 ABSTRACTS The 29th International Conference on Photochemistry Boulder, Colorado July 21 – 26, 2019 PLENARY SPEAKER ABSTRACTS PLENARY PRESENTATIONS ICP2019 Code: Plenary Presenter: Hiroshi MASUHARA (TWN) Abstract Title: Optical Manipulation in Chemistry Co-Author(s): Abstract: Photochemistry and spectroscopy study various dynamics and mechanism of molecules and materials induced by their interaction with light. Optical force is another interaction between light and matter, which was experimentally confirmed by Lebedev in late 19th century. Utilizing this force Ashkin proposed as “Optical Tweezers” in 1986 and demonstrated high potential in application to bio-science, which was awarded Nobel Prize Physics last year. Optical force was also expected to control molecular motion in solution and eventually chemical reaction. In 1988 we started to explore new molecular phenomena characteristic of optical force by combining optical trapping with fluorescence, absorption, electrochemical, and ablation methods. We have studied on microparticles, microdroplets, nanoparticles, polymers, supramolecules, micelles, amino acids and proteins in solution and reported their unique behavior. The remarkable achievement was performed in 2007 when the trapping laser was focused at solution surface. Various amino acids are crystalized, giving one single crystal at the position where trapping laser is focused and at the time when irradiated. This optical manipulation at solution surface is extended to dielectric and gold nanoparticles and to glass/solution interface. The initially trapped polystyrene nanoparticles at the focus form their periodical structure and scatter/propagate the trapping laser, shifting trapping site outside of the focus and forming a single large circle assembly.
[Show full text]