Life Sciences Unit Services

Life sciences unit services

Central Laboratory, College of Science, King Saud University

S. Number Type of Analysis Instruments Last user No of lab. Dr. Mukhtar Scanning, Screening, interpretation and Transmission Electron Ahmed 1 photography of Biological and Nano AB 93 Microscopy (TEM) sample Shaban Roshdy Dr. Mukhtar 2 Samples cutting both semi-thin and Ultracut Ultra-microtome Ahmed AB 87/1 Shaban Roshdy Dr. Mukhtar Ahmed 3 Samples orientation and trimming TEM Tissue Trimer AB 87/1 Shaban Roshdy Dr. Mukhtar Making glass knives for both semi-thin and 4 Glass knife maker Ahmed AB 87/1 Ultracut Shaban Roshdy Dr. Mukhtar Ahmed 5 High speed dispersion for Nano samples Probe sonication AB 87/5 Shaban Roshdy Dr. Mukhtar Processed the samples before molding in 6 TEM Tissue Processing Ahmed AB 98 capsules Shaban Roshdy Dr. Mukhtar Staining for Ultracut sections before 7 TEM Strainer Ahmed AB 98 screening by TEM Shaban Roshdy Cutting ultra-sections for TEM Dr. Mukhtar 8 Diamond Knife AB 87/1 (70 -80 nano meter) Ahmed Produces images of a sample by scanning it Scanning Electron Dr. Omar 9 AB 87/4 with a focused beam of electrons. Microscopy (SEM) AbdElkader

Covering samples with Au and Pt To get Gold, Carbon and Dr. Omar 10 AB 87/4 good quality images. platinum Spattering AbdElkader

Analysis of fluorescent labelled samples of Confocal Spinning Dr. Salim 11 AA 63 mammalian and bacterial cells Disk Microscope Manoharadas

Determine concentration of DNA, RNA and Dr. Salim 12 Proteins based on absorbance at 260/280 Nano Drop AA 63 Manoharadas nm Amplifing a single copy or few copies of a Dr. Shaik 13 PCR AA 60 piece of DNA. Althaf Hussain

Detection of biological or physical events of Dr. Shaik 14 ELISA Reader AA 60 samples in microtiter plates. Althaf Hussain

Dr. Shaik 15 Provide Liquid Nitrogen Liquid Nitrogen Generator AA 60 Althaf Hussain

Centrifuge with Maximum rotating speed: Dr. Shaik 16 Ultra-centrifuge AA 60 50,000 rpm (254,345xg). Althaf Hussain

Dr. Shaik 17 Preservation of samples at -80 C Freezer -80 C AA 60 Althaf Hussain Sterilize the laboratory glassware, microbial Dr. Shaik 18 media, surgical instruments and medical Autoclave AA 60 Althaf Hussain waste. Dr. Shaik 19 Provide ice cubes Ice Maker AA 60 Althaf Hussain

Analyses blood cell parameters like WBC Dr. Shaik 20 Blood Cell Counter AA 60 count, RBC count and Platelet Count. Althaf Hussain Measurement of different chemicals and Chemistry Analyzer 21 other characteristics in a number of Dr. Javid Dar AA 60 BS 200 biological samples. Analyses immunoassays, complex genetic 22 Luminex Dr. Javid Dar AA 60 analysis, and enzymatic assays Quantitatively measure the amplification of 23 Real Time PCR Dr. Javid Dar AA 60 DNA using fluorescent probes. Fluorescent western blotting, Gel Doc/Western blotting 24 chemiluminescence detection, and general Dr. Javid Dar AA 60 appartus gel documentation applications. Centrifuge for small tubes (0.2 ml - 2.0 ml) 25 Microcentrifuge Dr. Javid Dar AA 63 with up to 12000 Rpm Resolve separate components of a protein 26 PAGE System Dr. Javid Dar AA 60 mixture based on their size. For Cell lines and Tissue cultures Tissue culture cabinate Dr. Shaik 27 AA 63 experiments and CO2 incubator Althaf Hussain

28 Measuring the pH for solutions pH meter Dr. Javid Dar AA 63

Centrifuging around 5000 rpm at cooling Cooling Centrifuge 29 Dr. Javid Dar AA 63 temperature (Thermo Sci.)

Electrophoresis of nucleic acids and Automated Dr. Salim 30 AA60 proteins Electrophoresis System Manoharadas

Maintain optimal temperature for critical Dr. Salim 31 Water Bath AA 63 enzyme reactions Manoharadas

Long time storage of bacterial/mammalian Dr. Salim 32 Big Freezer -80 C AA 63 cells as glycerol stock Manoharadas

Dr. Salim 33 Generation of solid carbon dioxide Dry Ice Maker AA 63 Manoharadas