Cell Guardian - an Innovative Cryopreservation Medium That Defies Traditional Cell Preservation

Cell Guardian - an innovative cryopreservation medium that defies traditional cell preservation methodology. Simply, resuspend your cells in Cell Guardian then place the cryovial in a test tube rack or cardboard box overnight in a -80°C freezer. One step and done! Cells can be kept at -80°C for long term storage or transferred to liquid nitrogen. Traditional cell freezing protocols for mammalian cells Go use dimethyl sulfoxide (DMSO) or glycerol as a cryoprotectant in culture medium with or without serum. Vials of suspended cells are then slowly cooled at a rate of 1 -3°C per minute in a rate-controlled freezer. There are many other methods to freeze cells using less specialized equipment but all use the same strategy, a slow decrease in temperature over time.

Benefits

➣ 80-95% viable after 1 year storage

➣ Cells placed directly into -80°C freezer

➣ No more step-down freezing process

➣ Only requires cyrovials and a test tube rack ➣ Long term storage at -80°C or liquid nitrogen

➣ High cell viability

➣ Serum-free

➣ No animal-derived components

➣ Low viscosity for easy cell resuspension

➣ Reliable

➣ High quality

➣ Ideal for cell banking

➣ FDA Master Drug File – filing in process

Cell Guardian has been proven to work with over 170 types of cells – adherent or suspension cells, primary cells, stem cells, human, mouse, rat, dog, hamster, monkey, pig, cow, goat, and rabbit cell lines. Cell viability is comparable or better than commercially available cryopreservation medium (figure 1-4).

HeLa HUVEC CHO-K1 THP-1

Cell Guardian Medium with Medium Cell Guardian Medium with Medium Cell Guardian Medium with Medium Cell Guardian Medium with Medium serum without serum serum without serum serum without serum serum without serum Figure 1* Figure 2* Figure 3* Figure 4* * Percent viability determined after cells were stored frozen at -80°C for 1 year.